RU2014120465A - Функционализированные наночастицы для внутриклеточной доставки биологически активных молекул - Google Patents
Функционализированные наночастицы для внутриклеточной доставки биологически активных молекул Download PDFInfo
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- 239000002105 nanoparticle Substances 0.000 title claims abstract 24
- 230000001413 cellular effect Effects 0.000 claims abstract 13
- 210000004027 cell Anatomy 0.000 claims abstract 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 12
- 210000004962 mammalian cell Anatomy 0.000 claims abstract 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract 10
- 102000004169 proteins and genes Human genes 0.000 claims abstract 10
- 238000000034 method Methods 0.000 claims abstract 7
- 230000000975 bioactive effect Effects 0.000 claims abstract 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract 4
- 108091023040 Transcription factor Proteins 0.000 claims abstract 4
- 102000040945 Transcription factor Human genes 0.000 claims abstract 4
- 150000001413 amino acids Chemical class 0.000 claims abstract 4
- 239000011248 coating agent Substances 0.000 claims abstract 4
- 238000000576 coating method Methods 0.000 claims abstract 4
- 125000000524 functional group Chemical group 0.000 claims abstract 4
- 239000012528 membrane Substances 0.000 claims abstract 4
- 229920000642 polymer Polymers 0.000 claims abstract 4
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 claims abstract 2
- 230000003915 cell function Effects 0.000 claims abstract 2
- 229910052742 iron Inorganic materials 0.000 claims abstract 2
- 101150111214 lin-28 gene Proteins 0.000 claims abstract 2
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 claims 1
- 230000000877 morphologic effect Effects 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 claims 1
- 230000002062 proliferating effect Effects 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- 230000004083 survival effect Effects 0.000 claims 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
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- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
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- B60R22/34—Belt retractors, e.g. reels
- B60R22/341—Belt retractors, e.g. reels comprising energy-absorbing means
- B60R22/3413—Belt retractors, e.g. reels comprising energy-absorbing means operating between belt reel and retractor frame
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K38/00—Medicinal preparations containing peptides
- A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
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- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
- A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
- A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
- A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
- A61K47/6931—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle the material constituting the nanoparticle being a polymer
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- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
- A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
- A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
- A61K47/6931—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle the material constituting the nanoparticle being a polymer
- A61K47/6939—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle the material constituting the nanoparticle being a polymer the polymer being a polysaccharide, e.g. starch, chitosan, chitin, cellulose or pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
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- B60—VEHICLES IN GENERAL
- B60R—VEHICLES, VEHICLE FITTINGS, OR VEHICLE PARTS, NOT OTHERWISE PROVIDED FOR
- B60R22/00—Safety belts or body harnesses in vehicles
- B60R22/28—Safety belts or body harnesses in vehicles incorporating energy-absorbing devices
- B60R2022/286—Safety belts or body harnesses in vehicles incorporating energy-absorbing devices using deformation of material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B60—VEHICLES IN GENERAL
- B60R—VEHICLES, VEHICLE FITTINGS, OR VEHICLE PARTS, NOT OTHERWISE PROVIDED FOR
- B60R22/00—Safety belts or body harnesses in vehicles
- B60R22/28—Safety belts or body harnesses in vehicles incorporating energy-absorbing devices
- B60R2022/289—Energy-absorption curves
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
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- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
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Abstract
1. Функционализированная биосовместимая наночастица, способная проникать сквозь мембрану клетки млекопитающего и доставлять множество биоактивных молекул для модулирования клеточной функции, включающая:центральную наночастицу с размером в интервале от 5 до 50 нм, которая имеет полимерное покрытие,множество функциональных групп, ковалентно присоединенных к полимерному покрытию, где множество биоактивных молекул присоединено к множеству функциональных групп, и где множество биоактивных молекул включает, по меньшей мере, пептид или белок, и где пептид способен проникать сквозь мембрану клетки млекопитающего и проникать в клетку, и где белок способен обеспечивать новую функциональность внутри клетки2. Функционализированная биосовместимая наночастица по п. 1, где наночастица включает железо.3. Функционализированная биосовместимая наночастица по п. 2, где пептид присоединен к белку.4. Функционализированная биосовместимая наночастица по п. 3, где пептид и белок каждый соединен с наночастицей посредством одной или нескольких вставленных линкерных молекул.5. Функционализированная биосовместимая наночастица по п. 1, где пептид включает от пяти до девяти основных аминокислот.6. Функционализированная биосовместимая наночастица по п. 1, где пептид включает девять или более основных аминокислот.7. Функционализированная биосовместимая наночастица по п. 5, где белок представляет собой транскрипционный фактор.8. Функционализированная биосовместимая наночастица по п. 7, где транскрипционный фактор выбран из группы, состоящей из Oct4, Sox2, Nanog, Lin28, сМус и Klf4.9. Способ изменения клеточной функциональности внутри клетки млекоп
Claims (14)
1. Функционализированная биосовместимая наночастица, способная проникать сквозь мембрану клетки млекопитающего и доставлять множество биоактивных молекул для модулирования клеточной функции, включающая:
центральную наночастицу с размером в интервале от 5 до 50 нм, которая имеет полимерное покрытие,
множество функциональных групп, ковалентно присоединенных к полимерному покрытию, где множество биоактивных молекул присоединено к множеству функциональных групп, и где множество биоактивных молекул включает, по меньшей мере, пептид или белок, и где пептид способен проникать сквозь мембрану клетки млекопитающего и проникать в клетку, и где белок способен обеспечивать новую функциональность внутри клетки
2. Функционализированная биосовместимая наночастица по п. 1, где наночастица включает железо.
3. Функционализированная биосовместимая наночастица по п. 2, где пептид присоединен к белку.
4. Функционализированная биосовместимая наночастица по п. 3, где пептид и белок каждый соединен с наночастицей посредством одной или нескольких вставленных линкерных молекул.
5. Функционализированная биосовместимая наночастица по п. 1, где пептид включает от пяти до девяти основных аминокислот.
6. Функционализированная биосовместимая наночастица по п. 1, где пептид включает девять или более основных аминокислот.
7. Функционализированная биосовместимая наночастица по п. 5, где белок представляет собой транскрипционный фактор.
8. Функционализированная биосовместимая наночастица по п. 7, где транскрипционный фактор выбран из группы, состоящей из Oct4, Sox2, Nanog, Lin28, сМус и Klf4.
9. Способ изменения клеточной функциональности внутри клетки млекопитающего, включающий введение эффективного количества функционализированной биосовместимой наночастицы по п. 1 в клетку и изменение клеточной функциональности внутри клетки.
10. Способ изменения клеточной функциональности внутри клетки млекопитающего по п. 9, где изменение клеточной функциональности включает изменение физико-химического свойства клетки.
11. Способ изменения клеточной функциональности внутри клетки млекопитающего по п. 9, где изменение клеточной функциональности включает изменение пролиферативного свойства клетки.
12. Способ изменения клеточной функциональности внутри клетки млекопитающего по п. 9, где изменение клеточной функциональности включает изменение способности выживаемости клетки.
13. Способ изменения клеточной функциональности внутри клетки млекопитающего по п. 9, где изменение клеточной функциональности включает изменение морфологического фенотипического свойства клетки.
14. Способ изменения клеточной функциональности внутри клетки млекопитающего по п.9, где изменение клеточной функциональности включает приобретенную способность клетки создавать новый клеточный тип, включающий стволовую клетку или более специализированный клеточный тип.
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US201161550213P | 2011-10-21 | 2011-10-21 | |
US61/550,213 | 2011-10-21 | ||
PCT/US2012/061391 WO2013059831A1 (en) | 2011-10-21 | 2012-10-22 | Functionalized nanoparticles for intracellular delivery of biologically active molecules |
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RU2014120465/15A RU2014120465A (ru) | 2011-10-21 | 2012-10-22 | Функционализированные наночастицы для внутриклеточной доставки биологически активных молекул |
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EP (2) | EP2769217A4 (ru) |
JP (3) | JP2014532628A (ru) |
KR (3) | KR20200040924A (ru) |
CN (2) | CN104094119A (ru) |
AU (3) | AU2012325723A1 (ru) |
BR (1) | BR112014009753B1 (ru) |
CA (2) | CA2853128C (ru) |
HK (1) | HK1201089A1 (ru) |
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RU (2) | RU2018135567A (ru) |
SG (2) | SG11201401658SA (ru) |
WO (1) | WO2013059831A1 (ru) |
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KR20200040924A (ko) * | 2011-10-21 | 2020-04-20 | 스템제닉스 인코포레이티드 | 생물학적 활성 분자의 세포내 전달을 위한 작용화 나노입자 |
CN105517533A (zh) | 2013-03-01 | 2016-04-20 | 康德生物医疗技术公司 | 治疗线粒体疾病的方法 |
DK2961420T3 (da) * | 2013-03-01 | 2019-10-07 | Stealth Biotherapeutics Corp | Fremgangsmåder og sammensætninger til forebyggelsen eller behandlingen af barth syndrom |
CA2916977A1 (en) | 2013-06-26 | 2014-12-31 | Stealth Biotherapeutics Corp | Methods and compositions for detecting and diagnosing diseases and conditions |
US11306326B2 (en) | 2013-08-23 | 2022-04-19 | Rutgers, The State University Of New Jersey | Biologically active synthetic nanoparticle constructs and methods of use thereof |
US10100332B2 (en) | 2013-08-23 | 2018-10-16 | Rutgers, The State University Of New Jersey | Biologically active synthetic nanoparticle constructs and methods of use thereof |
US20170015975A1 (en) * | 2014-02-10 | 2017-01-19 | Nvigen, Inc. | Cell modulation nanocomposition, and methods of use |
CN105440112A (zh) * | 2015-12-07 | 2016-03-30 | 国家纳米科学中心 | 多肽-白蛋白偶联药物、其制备方法及应用 |
CN114634908A (zh) * | 2016-06-03 | 2022-06-17 | 斯特姆詹尼克斯公司 | 用功能化纳米颗粒将人类体细胞直接重编程为选定的(预定的)分化细胞 |
WO2017210666A2 (en) * | 2016-06-03 | 2017-12-07 | Stemgenics, Inc. | Functionalized nanoparticles for the intracellular delivery of biologically active molecules and methods for their manufacture and use |
US20190233820A1 (en) * | 2016-10-11 | 2019-08-01 | Stemgenics, Inc. | Nanoparticles functionalized with gene editing tools and related methods |
US11491114B2 (en) | 2016-10-12 | 2022-11-08 | Curioralrx, Llc | Formulations for enteric delivery of therapeutic agents |
JOP20190248A1 (ar) | 2017-04-21 | 2019-10-20 | Amgen Inc | بروتينات ربط مولد ضد trem2 واستخداماته |
US11701433B2 (en) | 2017-06-05 | 2023-07-18 | The Regents Of The University Of Michigan | Complexes for delivery of antigenic peptides |
CN108287235B (zh) * | 2018-02-07 | 2021-03-09 | 常州天地人和生物科技有限公司 | 一种高效、稳定的磁性免疫微球的制备和应用 |
US20200326325A1 (en) | 2019-04-12 | 2020-10-15 | Lisa Diamond | Nanosensor chip with compound nanopores |
CN110642876A (zh) * | 2019-10-10 | 2020-01-03 | 南京市口腔医院 | 半胱氨酸修饰金纳米颗粒和制备方法、应用及促进骨组织再生产品 |
CN112472685B (zh) * | 2020-12-10 | 2023-03-24 | 哈尔滨工业大学 | 一种杂化中性粒细胞机器人的制备方法 |
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EP1216060A2 (de) * | 1999-09-14 | 2002-06-26 | Biomedical Apherese Systeme GmbH | Magnetische nanoteilchen mit biochemischer wirksamkeit und verfahren zu ihrer herstellung sowie ihre verwendung |
US7329638B2 (en) * | 2003-04-30 | 2008-02-12 | The Regents Of The University Of Michigan | Drug delivery compositions |
WO2007024323A2 (en) * | 2005-06-17 | 2007-03-01 | The University Of North Carolina At Chapel Hill | Nanoparticle fabrication methods, systems, and materials |
CN101389314A (zh) * | 2005-03-14 | 2009-03-18 | 得克萨斯大学体系董事会 | 生物活性fus1肽和纳米颗粒-多肽复合物 |
JP2009501237A (ja) * | 2005-03-14 | 2009-01-15 | ボード オブ リージェンツ オブ ザ ユニバーシティー オブ テキサス システム | 生物活性fus1ペプチドおよびナノ粒子−ポリペプチド複合体 |
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KR100925689B1 (ko) | 2007-07-25 | 2009-11-10 | 한국생명공학연구원 | 항체와 나노입자를 세포 내로 동시에 전달할 수 있는다기능성 단백질 |
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KR100951719B1 (ko) | 2007-10-02 | 2010-04-07 | 재단법인서울대학교산학협력재단 | 세포투과성 펩타이드와 형광표지 자성나노입자의 복합체 및그 용도 |
US20110190729A1 (en) * | 2007-11-30 | 2011-08-04 | Cytomatrix Pty Ltd | Methods of inducing pluripotency involving sox2 protein |
ES2951646T3 (es) | 2008-02-21 | 2023-10-24 | Burnham Institute For Medical Res | Métodos y composiciones relacionados con péptidos y proteínas con elementos C-terminales |
AU2009329893A1 (en) * | 2008-12-23 | 2011-06-30 | Vivoscript, Inc. | Compositions and methods for re-programming cells without genetic modification |
KR101701534B1 (ko) * | 2009-04-07 | 2017-02-01 | 다우 아그로사이언시즈 엘엘씨 | 서열 특이적 뉴클레아제의 나노입자 매개 전달 |
KR20200040924A (ko) * | 2011-10-21 | 2020-04-20 | 스템제닉스 인코포레이티드 | 생물학적 활성 분자의 세포내 전달을 위한 작용화 나노입자 |
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2012
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- 2012-10-22 CN CN201280063870.2A patent/CN104094119A/zh active Pending
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- 2012-10-22 BR BR112014009753-4A patent/BR112014009753B1/pt active IP Right Grant
- 2012-10-22 JP JP2014537369A patent/JP2014532628A/ja not_active Withdrawn
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- 2012-10-22 WO PCT/US2012/061391 patent/WO2013059831A1/en active Application Filing
- 2012-10-22 RU RU2018135567A patent/RU2018135567A/ru not_active Application Discontinuation
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- 2012-10-22 EP EP18160010.7A patent/EP3400956A1/en not_active Withdrawn
- 2012-10-22 AU AU2012325723A patent/AU2012325723A1/en not_active Abandoned
- 2012-10-22 US US14/353,280 patent/US9675708B2/en active Active - Reinstated
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2015
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Also Published As
Publication number | Publication date |
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JP2014532628A (ja) | 2014-12-08 |
KR20150001711A (ko) | 2015-01-06 |
KR20190077124A (ko) | 2019-07-02 |
RU2018135567A (ru) | 2018-11-15 |
WO2013059831A1 (en) | 2013-04-25 |
JP2018184485A (ja) | 2018-11-22 |
MX367656B (es) | 2019-08-29 |
HK1201089A1 (en) | 2015-08-21 |
AU2012325723A1 (en) | 2014-05-15 |
MX2018010696A (es) | 2020-09-02 |
SG11201401658SA (en) | 2014-07-30 |
EP3400956A1 (en) | 2018-11-14 |
US20140342004A1 (en) | 2014-11-20 |
EP2769217A4 (en) | 2015-06-03 |
CA2938661A1 (en) | 2013-04-25 |
SG10201601746TA (en) | 2016-04-28 |
CN106822868A (zh) | 2017-06-13 |
JP6560302B2 (ja) | 2019-08-14 |
MX2014004778A (es) | 2014-10-17 |
BR112014009753A2 (pt) | 2017-04-25 |
CN104094119A (zh) | 2014-10-08 |
JP2017165781A (ja) | 2017-09-21 |
AU2018203848A1 (en) | 2018-06-21 |
AU2020223737A1 (en) | 2020-09-17 |
US9675708B2 (en) | 2017-06-13 |
EP2769217A1 (en) | 2014-08-27 |
CA2853128C (en) | 2016-09-27 |
CA2853128A1 (en) | 2013-04-25 |
IN2014DN03224A (ru) | 2015-05-22 |
BR112014009753B1 (pt) | 2020-09-15 |
KR20200040924A (ko) | 2020-04-20 |
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