RU2006105626A - Определение последовательности полинуклеотида - Google Patents
Определение последовательности полинуклеотида Download PDFInfo
- Publication number
- RU2006105626A RU2006105626A RU2006105626/13A RU2006105626A RU2006105626A RU 2006105626 A RU2006105626 A RU 2006105626A RU 2006105626/13 A RU2006105626/13 A RU 2006105626/13A RU 2006105626 A RU2006105626 A RU 2006105626A RU 2006105626 A RU2006105626 A RU 2006105626A
- Authority
- RU
- Russia
- Prior art keywords
- target polynucleotide
- polymerase
- measuring
- sequence
- nucleotides
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/113—Time
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Claims (13)
1. Способ идентификации последовательности целевого полинуклеотида, предусматривающий
(i) приведение в контакт целевого полинуклеотида с полимеразным ферментом и одним из нуклеотидов А, Т(U), G и С в условиях, подходящих для прохождения полимеразной реакции,
(ii) измерение времени, затраченного полимеразой на то, чтобы она связалась и затем отделилась от целевого полинуклеотида для того, чтобы таким образом определить, включила ли полимераза нуклеотид в целевой полинуклеотид,
(iii) необязательно повторение этапов (i) и (ii) с дополнительными нуклеотидами для того, чтобы таким образом идентифицировать последовательность целевого полинуклеотида.
2. Способ идентификации мутации в целевом полинуклеотиде, включающий в себя этапы, на которых
(i) целевой полинуклеотид приводят в контакт с полимеразным ферментом и одним из нуклеотидов А, Т(U), G и С в условиях, подходящих для прохождения полимеразной реакции, и
(ii) измеряют время, затраченное полимеразой на связывание и последующее отделение от целевого полинуклеотида, чтобы таким образом идентифицировать, включила ли полимераза нуклеотид в целевой полинуклеотид, и исходя из природной последовательности мишени определить, существует ли мутация.
3. Способ по п.1 или 2, в котором этапы (i)-(ii) выполняют по очереди с каждым из различных нуклеотидов до тех пор, пока не обнаружат включение.
4. Способ по п.1 или 2, в котором целевой полинуклеотид иммобилизован на материале подложки.
5. Способ по п.1 или 2, в котором множество целевых полинуклеотидов иммобилизованы на материале подложки.
6. Способ по п.1 или 2, в котором этап (ii) выполняют путем измерения приложенного излучения.
7. Способ по п.1 или 2, в котором этап (ii) выполняют путем измерения комбинационного рассеяния.
8. Способ по п.1 или 2, в котором этап (ii) выполняют путем приложения поверхностной электромагнитной волны.
9. Способ по п.8, в котором поверхностная электромагнитная волна представляет собой поверхностную плазменную волну.
10. Способ по п.1 или 2, в котором обнаружение выполняют путем измерения поверхностной электромагнитной волны.
11. Способ по п.1 или 2, в котором полимераза содержит детектируемую метку, прикрепленную к ней.
12. Способ по п.11, в котором метка представляет собой флуорофор.
13. Способ по п.11 в той части, в которой он зависит от любого из пп.1-6, в котором полимераза дополнительно содержит метку в виде донора энергии или метку в виде акцептора энергии и в котором этап (ii) выполняют путем измерения переноса энергии между флуорофором и донором или акцептором энергии.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0317343.2A GB0317343D0 (en) | 2003-07-24 | 2003-07-24 | Polynucleotide sequencing |
| GB0317343.2 | 2003-07-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2006105626A true RU2006105626A (ru) | 2006-08-10 |
Family
ID=27772590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2006105626/13A RU2006105626A (ru) | 2003-07-24 | 2004-07-26 | Определение последовательности полинуклеотида |
Country Status (21)
| Country | Link |
|---|---|
| US (3) | US7604963B2 (ru) |
| EP (1) | EP1649051B1 (ru) |
| JP (1) | JP4394684B2 (ru) |
| KR (1) | KR20060052863A (ru) |
| CN (1) | CN1852991A (ru) |
| AT (1) | ATE388243T1 (ru) |
| AU (1) | AU2004259893A1 (ru) |
| BR (1) | BRPI0412813A (ru) |
| CA (2) | CA2780958C (ru) |
| DE (1) | DE602004012273T2 (ru) |
| DK (1) | DK1649051T3 (ru) |
| ES (1) | ES2303083T3 (ru) |
| GB (1) | GB0317343D0 (ru) |
| IL (1) | IL173314A0 (ru) |
| IS (1) | IS8314A (ru) |
| MX (1) | MXPA06000962A (ru) |
| PL (1) | PL1649051T3 (ru) |
| PT (1) | PT1649051E (ru) |
| RU (1) | RU2006105626A (ru) |
| SI (1) | SI1649051T1 (ru) |
| WO (1) | WO2005010210A2 (ru) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7008766B1 (en) * | 1997-07-28 | 2006-03-07 | Medical Biosystems, Ltd. | Nucleic acid sequence analysis |
| GB9923644D0 (en) | 1999-10-06 | 1999-12-08 | Medical Biosystems Ltd | DNA sequencing |
| GB0112238D0 (en) * | 2001-05-18 | 2001-07-11 | Medical Biosystems Ltd | Sequencing method |
| JP2004347621A (ja) * | 2003-04-24 | 2004-12-09 | Dainippon Printing Co Ltd | 透過型スクリーン |
| JP4431549B2 (ja) | 2006-05-31 | 2010-03-17 | 株式会社日立ハイテクノロジーズ | 蛍光分析装置 |
| GB0721340D0 (en) * | 2007-10-30 | 2007-12-12 | Isis Innovation | Polymerase-based single-molecule DNA sequencing |
| JP4982523B2 (ja) * | 2009-05-11 | 2012-07-25 | 株式会社日立ハイテクノロジーズ | 蛍光分析方法，蛍光分析装置及び画像検出方法 |
| US9353412B2 (en) | 2010-06-18 | 2016-05-31 | Illumina, Inc. | Conformational probes and methods for sequencing nucleic acids |
| US9279154B2 (en) * | 2011-12-21 | 2016-03-08 | Illumina, Inc. | Apparatus and methods for kinetic analysis and determination of nucleic acid sequences |
| US9593371B2 (en) * | 2013-12-27 | 2017-03-14 | Intel Corporation | Integrated photonic electronic sensor arrays for nucleic acid sequencing |
| US9146248B2 (en) | 2013-03-14 | 2015-09-29 | Intelligent Bio-Systems, Inc. | Apparatus and methods for purging flow cells in nucleic acid sequencing instruments |
| US9591268B2 (en) | 2013-03-15 | 2017-03-07 | Qiagen Waltham, Inc. | Flow cell alignment methods and systems |
| US10077470B2 (en) | 2015-07-21 | 2018-09-18 | Omniome, Inc. | Nucleic acid sequencing methods and systems |
| CN109562142B (zh) * | 2016-04-04 | 2022-09-13 | 纽约哥伦比亚大学董事会 | 使用核苷酸类似物和拉曼检测的dna合成测序 |
| CA3021769C (en) | 2016-04-29 | 2021-11-23 | Omniome, Inc. | Sequencing method employing ternary complex destabilization to identify cognate nucleotides |
| EP4039824A1 (en) | 2016-08-15 | 2022-08-10 | Pacific Biosciences of California, Inc. | Method and system for sequencing nucleic acids |
| WO2018034780A1 (en) | 2016-08-15 | 2018-02-22 | Omniome, Inc. | Sequencing method for rapid identification and processing of cognate nucleotide pairs |
| AU2017386515B2 (en) | 2016-12-30 | 2021-08-19 | Pacific Biosciences Of California, Inc. | Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides |
| AU2018210944B2 (en) | 2017-01-20 | 2021-07-22 | Pacific Biosciences Of California, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
| EP3571313B1 (en) | 2017-01-20 | 2020-12-23 | Omniome, Inc. | Allele-specific capture of nucleic acids |
| US9932631B1 (en) | 2017-09-11 | 2018-04-03 | Omniome, Inc. | Genotyping by polymerase binding |
| US10161003B2 (en) | 2017-04-25 | 2018-12-25 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
| US9951385B1 (en) | 2017-04-25 | 2018-04-24 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
| CA3079411C (en) | 2017-10-19 | 2023-12-05 | Omniome, Inc. | Simultaneous background reduction and complex stabilization in binding assay workflows |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6416952B1 (en) * | 1989-06-07 | 2002-07-09 | Affymetrix, Inc. | Photolithographic and other means for manufacturing arrays |
| CA2658290C (en) * | 1996-06-04 | 2012-04-10 | University Of Utah Research Foundation | Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna |
| US7008766B1 (en) | 1997-07-28 | 2006-03-07 | Medical Biosystems, Ltd. | Nucleic acid sequence analysis |
| CN1245218A (zh) | 1998-08-19 | 2000-02-23 | 中国人民解放军军事医学科学院放射医学研究所 | 一种固相逐个碱基核酸分析方法和仪器 |
| DK1159453T3 (da) | 1999-03-10 | 2008-10-06 | Asm Scient Inc | Fremgangsmåde til direkte nukleinsyresekventering |
| US7056661B2 (en) * | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| AU7086800A (en) | 1999-08-30 | 2001-03-26 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | High speed parallel molecular nucleic acid sequencing |
| US6908736B1 (en) | 1999-10-06 | 2005-06-21 | Medical Biosystems, Ltd. | DNA sequencing method |
| GB9923644D0 (en) | 1999-10-06 | 1999-12-08 | Medical Biosystems Ltd | DNA sequencing |
| GB0112238D0 (en) | 2001-05-18 | 2001-07-11 | Medical Biosystems Ltd | Sequencing method |
| US6982165B2 (en) * | 2001-09-24 | 2006-01-03 | Intel Corporation | Nucleic acid sequencing by raman monitoring of molecular deconstruction |
| GB0413082D0 (en) | 2004-06-11 | 2004-07-14 | Medical Biosystems Ltd | Method |
-
2003
- 2003-07-24 GB GBGB0317343.2A patent/GB0317343D0/en not_active Ceased
-
2004
- 2004-07-26 SI SI200430736T patent/SI1649051T1/sl unknown
- 2004-07-26 DK DK04743561T patent/DK1649051T3/da active
- 2004-07-26 AU AU2004259893A patent/AU2004259893A1/en not_active Abandoned
- 2004-07-26 DE DE602004012273T patent/DE602004012273T2/de not_active Expired - Lifetime
- 2004-07-26 WO PCT/GB2004/003232 patent/WO2005010210A2/en active IP Right Grant
- 2004-07-26 PT PT04743561T patent/PT1649051E/pt unknown
- 2004-07-26 AT AT04743561T patent/ATE388243T1/de not_active IP Right Cessation
- 2004-07-26 KR KR1020067001539A patent/KR20060052863A/ko not_active Application Discontinuation
- 2004-07-26 JP JP2006520906A patent/JP4394684B2/ja not_active Expired - Fee Related
- 2004-07-26 MX MXPA06000962A patent/MXPA06000962A/es active IP Right Grant
- 2004-07-26 US US10/565,750 patent/US7604963B2/en not_active Expired - Lifetime
- 2004-07-26 CN CNA2004800269313A patent/CN1852991A/zh active Pending
- 2004-07-26 RU RU2006105626/13A patent/RU2006105626A/ru not_active Application Discontinuation
- 2004-07-26 EP EP04743561A patent/EP1649051B1/en not_active Expired - Lifetime
- 2004-07-26 ES ES04743561T patent/ES2303083T3/es not_active Expired - Lifetime
- 2004-07-26 PL PL04743561T patent/PL1649051T3/pl unknown
- 2004-07-26 CA CA2780958A patent/CA2780958C/en not_active Expired - Fee Related
- 2004-07-26 BR BRPI0412813-3A patent/BRPI0412813A/pt not_active IP Right Cessation
- 2004-07-26 CA CA2533579A patent/CA2533579C/en not_active Expired - Fee Related
-
2006
- 2006-01-23 IL IL173314A patent/IL173314A0/en unknown
- 2006-02-20 IS IS8314A patent/IS8314A/is unknown
-
2009
- 2009-10-16 US US12/580,596 patent/US7888073B2/en not_active Expired - Lifetime
-
2011
- 2011-01-05 US US12/984,822 patent/US8592183B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US7888073B2 (en) | 2011-02-15 |
| US7604963B2 (en) | 2009-10-20 |
| DE602004012273D1 (de) | 2008-04-17 |
| JP4394684B2 (ja) | 2010-01-06 |
| US20080070236A1 (en) | 2008-03-20 |
| US8592183B2 (en) | 2013-11-26 |
| CA2533579A1 (en) | 2005-02-03 |
| CA2780958C (en) | 2016-10-25 |
| JP2006528485A (ja) | 2006-12-21 |
| WO2005010210A2 (en) | 2005-02-03 |
| EP1649051B1 (en) | 2008-03-05 |
| ATE388243T1 (de) | 2008-03-15 |
| KR20060052863A (ko) | 2006-05-19 |
| US20110097729A1 (en) | 2011-04-28 |
| PT1649051E (pt) | 2008-05-26 |
| BRPI0412813A (pt) | 2006-09-26 |
| US20100279288A1 (en) | 2010-11-04 |
| WO2005010210A3 (en) | 2005-03-24 |
| CN1852991A (zh) | 2006-10-25 |
| DE602004012273T2 (de) | 2009-04-30 |
| IL173314A0 (en) | 2006-06-11 |
| IS8314A (is) | 2006-02-20 |
| PL1649051T3 (pl) | 2008-12-31 |
| ES2303083T3 (es) | 2008-08-01 |
| DK1649051T3 (da) | 2008-07-07 |
| CA2780958A1 (en) | 2005-02-03 |
| GB0317343D0 (en) | 2003-08-27 |
| EP1649051A2 (en) | 2006-04-26 |
| MXPA06000962A (es) | 2006-03-30 |
| SI1649051T1 (sl) | 2008-10-31 |
| AU2004259893A1 (en) | 2005-02-03 |
| CA2533579C (en) | 2012-09-11 |
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Legal Events
| Date | Code | Title | Description |
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| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20070627 |
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| FZ9A | Application not withdrawn (correction of the notice of withdrawal) |
Effective date: 20080305 |
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| FA94 | Acknowledgement of application withdrawn (non-payment of fees) |
Effective date: 20100118 |