RU2002133463A - Способ клеточной адгезиии и заживления ран - Google Patents
Способ клеточной адгезиии и заживления ранInfo
- Publication number
- RU2002133463A RU2002133463A RU2002133463/15A RU2002133463A RU2002133463A RU 2002133463 A RU2002133463 A RU 2002133463A RU 2002133463/15 A RU2002133463/15 A RU 2002133463/15A RU 2002133463 A RU2002133463 A RU 2002133463A RU 2002133463 A RU2002133463 A RU 2002133463A
- Authority
- RU
- Russia
- Prior art keywords
- βig
- domain
- fas
- recombinant protein
- copies
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 8
- 230000021164 cell adhesion Effects 0.000 title abstract 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract 9
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract 9
- 230000029663 wound healing Effects 0.000 claims abstract 4
- 101000802895 Dendroaspis angusticeps Fasciculin-1 Proteins 0.000 claims 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims 6
- 239000013604 expression vector Substances 0.000 claims 6
- 241000588724 Escherichia coli Species 0.000 claims 3
- 241000672609 Escherichia coli BL21 Species 0.000 claims 3
- 239000007787 solid Substances 0.000 claims 2
- 102000014914 Carrier Proteins Human genes 0.000 claims 1
- 108010078791 Carrier Proteins Proteins 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 206010052428 Wound Diseases 0.000 claims 1
- 208000027418 Wounds and injury Diseases 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 abstract 1
- 235000003704 aspartic acid Nutrition 0.000 abstract 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 102000006495 integrins Human genes 0.000 abstract 1
- 108010044426 integrins Proteins 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 229960000310 isoleucine Drugs 0.000 abstract 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 abstract 1
- 239000003357 wound healing promoting agent Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Claims (11)
1. Способ прикрепления клетки млекопитающего к рекомбинантному белку, включающему одну или более копий 2-го и/или 4-го домена fas-1 βig-h3, включающий следующие стадии: 1) получение рекомбинантного белка, включающего одну или более копий 2-го и/или 4-го домена fas-1 βig-h3, 2) покрытие рекомбинантным белком твердого носителя, и 3) нанесение клеток на покрытый белком твердый носитель.
2. Способ по п.1, отличающийся тем, что 2-й домен fas-1 βig-h3 включает аминокислотную последовательность 237-377 βig-h3.
3. Способ по п.1, отличающийся тем, что 4-й домен fas-1 βig-h3 включает аминокислотную последовательность 498-637 βig-h3.
4. Способ по п.1, отличающийся тем, что рекомбинантный белок включает аминокислотную последовательность 498-637 βig-h3 в четырех копиях.
5. Экспрессирующий вектор pβig-h3 D-II, кодирующий аминокислотную последовательность 237-377 βig-h3 и способный экспрессировать 2-й домен fas-1 βig-h3.
6. Штамм E.coli, трансформированный экспрессирующим вектором по п.5, обозначенный как E.coli BL21/Hisβ-g (регистрационный номер КСТС 0905ВР).
7. Экспрессирующий вектор рβig-h3 D-IV, кодирующий аминокислотную последовательность 498-637 βig-h3 и способный экспрессировать 4-й домен fas-1 βig-h3.
8. Штамм E.coli, трансформированный экспрессирующим вектором по п.7, обозначенный как E.coli BL21/Hisβ-е (регистрационный номер КСТС 0904ВР).
9. Экспрессирующий вектор pβig-h3 D-IV 4Х, экспрессирующий рекомбинантный белок, включающий аминокислотную последовательность 498-637 βig-h3 в четырех копиях.
10. Штамм E.coli, трансформированный экспрессирующим вектором по п.9, обозначенный как E.coli BL21/Hisβ-е4х (регистрационный номер КСТС 0909ВР).
11. Способ заживления ран у млекопитающего с помощью рекомбинантного белка, включающего одну или более копий 2-го и/или 4-го домена fas-1 βig-h3, включающий следующие стадии: 1) получение рекомбинантного белка, включающего одну или более копий 2-го и/или 4-го домена fas-1 βig-h3, 2) смешивание рекомбинантного белка с основанием, и 3) нанесение смеси на раны.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR2000/25662 | 2000-05-13 | ||
| KR10-2000-0025662A KR100385293B1 (ko) | 2000-05-13 | 2000-05-13 | βｉｇ-ｈ3의 일부 도메인을 이용한 세포 부착 및 창상치유 방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2002133463A true RU2002133463A (ru) | 2004-03-27 |
| RU2234937C2 RU2234937C2 (ru) | 2004-08-27 |
Family
ID=19668565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2002133463/15A RU2234937C2 (ru) | 2000-05-13 | 2000-12-08 | Способ клеточной адгезии и заживления ран |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7396682B2 (ru) |
| EP (1) | EP1282434B1 (ru) |
| JP (1) | JP3795805B2 (ru) |
| KR (1) | KR100385293B1 (ru) |
| CN (1) | CN1209165C (ru) |
| AT (1) | ATE446765T1 (ru) |
| AU (2) | AU2027001A (ru) |
| CA (1) | CA2409506A1 (ru) |
| DE (1) | DE60043238D1 (ru) |
| IL (2) | IL152410A0 (ru) |
| RU (1) | RU2234937C2 (ru) |
| WO (1) | WO2001087327A1 (ru) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8258260B2 (en) * | 1970-02-11 | 2012-09-04 | Immatics Biotechnologies Gmbh | Tumor-associated peptides binding promiscuously to human leukocyte antigen (HLA) class II molecules |
| KR100378949B1 (ko) * | 2000-05-13 | 2003-04-08 | 주식회사 리젠 바이오텍 | 세포 부착, 확산 및 탈착 활성을 나타내는 펩타이드 및그의 유도체 |
| JP2005527813A (ja) * | 2002-04-19 | 2005-09-15 | リージェン バイオテック インコーポレーテッド | βｉｇ−ｈ３蛋白質の定量方法及びこれを利用した診断キット |
| WO2005099743A1 (en) * | 2004-04-13 | 2005-10-27 | Kyungpook National University Industry-Academic Cooperation Foundation | Novel use of a polypeptide comprising fas-1 domain |
| KR100635540B1 (ko) * | 2004-10-12 | 2006-10-18 | 경북대학교 산학협력단 | 신규한 염증성 질환의 예방 또는 치료용 약학적 조성물 |
| AU2007342353B2 (en) * | 2007-01-05 | 2012-06-28 | Helix Biomedix Inc. | Short bio-active peptides for cellular and immunological modulation |
| JP2012012344A (ja) * | 2010-07-01 | 2012-01-19 | Japan Health Science Foundation | 創傷治癒剤 |
| CN108586584A (zh) * | 2018-05-03 | 2018-09-28 | 杭州史迪姆生物科技有限公司 | 功能多肽及其用途 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4983581A (en) * | 1988-05-20 | 1991-01-08 | Institute Of Molecular Biology, Inc. | Wound healing composition of IGF-I and TGF-β |
| ES2178633T3 (es) * | 1990-05-30 | 2003-01-01 | Harvard College | Utilizacion concomitante del factor -i de crecimiento similar a insulina (en lo sucesivo "fci-i") y el factor de crecimiento transformante beta (en la sucesivo "fct-beta") para la fabricacion de un medicamento para la regeneracion de huesos en mamiferos. |
| HUT67319A (en) * | 1991-08-30 | 1995-03-28 | Life Medical Sciences Inc | Compositions for treating wounds |
| US5444164A (en) * | 1992-02-05 | 1995-08-22 | Bristol-Myers Squibb Company | TGF-β induced gene |
| US5599788A (en) * | 1994-07-01 | 1997-02-04 | Advanced Tissue Sciences | Method for accelerating skin wound healing with H3 protein |
| KR100378949B1 (ko) * | 2000-05-13 | 2003-04-08 | 주식회사 리젠 바이오텍 | 세포 부착, 확산 및 탈착 활성을 나타내는 펩타이드 및그의 유도체 |
-
2000
- 2000-05-13 KR KR10-2000-0025662A patent/KR100385293B1/ko not_active IP Right Cessation
- 2000-12-08 RU RU2002133463/15A patent/RU2234937C2/ru not_active IP Right Cessation
- 2000-12-08 CA CA002409506A patent/CA2409506A1/en not_active Abandoned
- 2000-12-08 IL IL15241000A patent/IL152410A0/xx unknown
- 2000-12-08 AU AU2027001A patent/AU2027001A/xx not_active Withdrawn
- 2000-12-08 EP EP00983525A patent/EP1282434B1/en not_active Expired - Lifetime
- 2000-12-08 CN CNB008195307A patent/CN1209165C/zh not_active Expired - Fee Related
- 2000-12-08 US US10/276,479 patent/US7396682B2/en not_active Expired - Fee Related
- 2000-12-08 AT AT00983525T patent/ATE446765T1/de not_active IP Right Cessation
- 2000-12-08 DE DE60043238T patent/DE60043238D1/de not_active Expired - Lifetime
- 2000-12-08 AU AU2001220270A patent/AU2001220270B2/en not_active Ceased
- 2000-12-08 JP JP2001583794A patent/JP3795805B2/ja not_active Expired - Fee Related
- 2000-12-08 WO PCT/KR2000/001428 patent/WO2001087327A1/en active Application Filing
-
2008
- 2008-03-31 US US12/060,056 patent/US20100331253A9/en not_active Abandoned
-
2009
- 2009-07-29 IL IL200132A patent/IL200132A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1282434A1 (en) | 2003-02-12 |
| US7396682B2 (en) | 2008-07-08 |
| RU2234937C2 (ru) | 2004-08-27 |
| US20040052767A1 (en) | 2004-03-18 |
| ATE446765T1 (de) | 2009-11-15 |
| EP1282434A4 (en) | 2004-12-08 |
| AU2001220270B2 (en) | 2004-09-30 |
| IL152410A0 (en) | 2003-05-29 |
| DE60043238D1 (de) | 2009-12-10 |
| CN1452493A (zh) | 2003-10-29 |
| JP3795805B2 (ja) | 2006-07-12 |
| JP2004521605A (ja) | 2004-07-22 |
| US20100331253A9 (en) | 2010-12-30 |
| CA2409506A1 (en) | 2001-11-22 |
| KR100385293B1 (ko) | 2003-05-23 |
| WO2001087327A1 (en) | 2001-11-22 |
| KR20010104176A (ko) | 2001-11-24 |
| CN1209165C (zh) | 2005-07-06 |
| AU2027001A (en) | 2001-11-26 |
| EP1282434B1 (en) | 2009-10-28 |
| IL200132A0 (en) | 2010-04-15 |
| US20080214463A1 (en) | 2008-09-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20101209 |