RU175863U1 - BOTTLE FOR CULTIVATION OF MICRO-ORGANISMS WITH REMOVABLE COVER - Google Patents

Abstract

The utility model relates to the field of medicine and biotechnology and can be used to cultivate rapidly and slowly growing microorganisms in solid nutrient media. The proposed version of the bottle is a container consisting of three parts (the bottom and top are glued during production). The bottle has the shape of a trapezoid. It has a rectangular hole for filling media and subsequent work with material and grown crops. On the back of the bottle there are sides for the possibility of vertical installation. The front wall, under the sampling hole, is located at an angle of 26 °. The upper part of the microorganism cultivation bottle with a removable cap is connected to the bottom using hot or cold cured adhesives. The removable cover has the shape of a trapezoid, on the front of which there is a handle in the form of a rectangular parallelepiped. All parts of the bottle are made of high grade polypropylene, polystyrene or styrene copolymer. A glidant has been added to the base material to facilitate movement of the lid.

The utility model allows expanding the possibilities of cultivating microorganisms on plate agar, prevents dehydration of the medium, provides access to the selection of colonies, and reduces the risk of contamination of crops.

Description

The utility model relates to the field of medicine and biotechnology and can be used for the cultivation of rapidly and slowly growing microorganisms in solid nutrient media.

The possibilities of modern microbiology make it possible to cultivate and identify whimsical and slowly growing microorganisms, such as non-tuberculous mycobacteria, nocardia, rhodococcus, and gordonium. The cultivation of such microorganisms using conventional Petri dishes significantly limits the possibilities of working with them, because in this case, a sufficiently rapid dehydration of the medium occurs, which can significantly affect its growth qualities. In vitro cultivation virtually eliminates the isolation of a pure culture of microorganisms during primary culture or requires laborious procedures for diluting the primary material.

At the same time, some microorganisms (non-tuberculous mycobacteria, nocardia, rhodococcus, fungi) give visible growth on solid nutrient media after 14 or more days. The use of standard Petri dishes for cultivation also increases the risk of contamination of the crop with extraneous microflora from the environment, due to the large area of the cup itself.

Thus, it is necessary to create conditions that, on the one hand, reduce the rate of dehydration of a dense medium, and on the other hand, provide the ability to work with grown colonies with maximum access for their selection and minimal risk of contamination.

The purpose of creating a utility model is to develop a bottle for the cultivation of microorganisms with a removable cap, which will expand the possibilities of cultivating slowly growing microorganisms on plate agar without premature dehydration of the medium. This goal is achieved as follows.

Brief description of the drawing. The accompanying description of the drawings shows the design of the bottle for the cultivation of microorganisms with a removable cap.

Implementation of a utility model. The proposed version of the bottle for the cultivation of microorganisms with a removable lid is a container consisting of three parts (the bottom and top are glued during production). The microorganism cultivation bottle with a removable lid has a trapezoid shape with a lower base in the form of a square with a side from 82.96 mm to 83.04 mm and an upper base in the form of a square with a side from 88.5 mm to 88.71 mm and an angle of inclination lower base 84 ° 27 '(Figure No. 1). It has a rectangular hole for filling media and subsequent work with material and grown crops with sides from 32.98 mm to 33.15 mm and sides from 73.9 mm to 74 mm. The microorganism cultivation bottle with a removable lid has a wall thickness in all sections, including sides for sliding the lid from 1.5 mm to 1.55 mm. The wall thickness sets the plane for gluing the upper part (Figure No. 2). The height of the sides for sliding from 2.9 mm to 2.97 mm. On the back of the vial for cultivating microorganisms with a removable cap (Figure No. 1) there are sides with a width of 1.96 mm to 2 mm for vertical installation. The front wall, under the sampling hole, is located at an angle of 26 °.

The upper part of the microorganism cultivation bottle with a removable cap has the shape of a trapezoid with a lower base in the form of a square with a side from 88.4 mm to 88.52 mm and an upper base in the form of a square with a side from 93.89 mm to 93.95 mm and an angle tilt at lower base 84 ° 27 '. The height of the sides for sliding from 2.9 mm to 2.97 mm, the thickness of the sides from 1.5 mm to 1.55 mm. For bonding of Figure No. 1 and Figure No. 2, hot curing adhesives can be used. When cold curing, apply the following types of adhesives: PU-2, L-4, K-153. LOXAMID OA SPEZIAL sliding additive is introduced into the main material in an amount of 0.2-0.5% of the total polymer mass to facilitate the movement of parts relative to each other. The removable cover (Figure No. 3) has a trapezoid shape with a lower base from 88.9 mm to 88.83 mm and an upper base from 93.59 mm to 93.64 mm and an inclination angle at the lower base of 84 ° 27 '. The radii at the junctions of the ribs are 4 mm. The width of the removable cover is from 2.22 mm to 2.3 mm. The radii at the junctions of the faces are 1 mm. On the front part is a handle in the form of a rectangular parallelepiped, having the following geometric parameters 9-9.05 × 2.95-3.05 × 5-5.05 mm.

All parts of the microorganism cultivation bottle with a removable lid are made of polypropylene 21060-16 grade GOST 26996-86, polystyrene GOST 20281-86 or styrene copolymer MSN-L GOST 12271-76.

The utility model allows expanding the possibilities of cultivation of slowly growing microorganisms on lamellar agar without premature dehydration of the medium.

INFORMATION SOURCES

1. Clinical laboratory diagnostics: national leadership: in 2 volumes - T.P. / Ed. V.V. Dolgova, V.V. Menshikov. - M.: GEOTAR-Media, 2013 .-- 808 p.

2. Guide to medical microbiology. General and sanitary microbiology. Book I / Coll. authors // Ed. A.S. Labinskaya, E.G. Volina. - M.: Publishing house BINOM, 2008. - 1080 p.

3. Methods of general bacterology: Per. from English / Ed. F. Gerhardt et al. - Moscow: Mir, 1983 .-- 536 p.

Claims (1)

A vial for cultivating microorganisms with a removable lid, characterized in that it is a container consisting of a glued bottom with an upper part and a removable lid, has a trapezoid shape, where the upper base is larger than the size of the lower base, with a rectangular opening on the upper base for bottling and subsequent work, on the back of the bottle there are sides for vertical installation of the bottle, the front wall under the hole is at an angle of 26 °, the upper part of the bottle it is connected to the bottom with a removable cap using hot or cold curing adhesives, the removable cap is also made in the form of a trapezoid, where the handle in the form of a rectangular parallelepiped is located on the front of the cap, while all parts of the bottle are made of the highest grade of polypropylene, polystyrene or styrene copolymer , a glidant additive in an amount of 0.2-0.5% of the total polymer mass was also introduced into the base material to facilitate the movement of the lid.

Images