RU175134U1 - BOTTLE FOR CULTIVATION OF MICRO-ORGANISMS - Google Patents

Abstract

The utility model relates to the field of medicine and biotechnology and can be used for the cultivation of rapidly and slowly growing microorganisms in solid nutrient media. The proposed version of the bottle for the cultivation of microorganisms consists of two parts (cup and body). The casing is a rectangular parallelepiped with a through hole of a rectangular section in size corresponding to the cup, which is placed in the casing. On the upper and lower walls there are sides for stacking bottles. The cup has the shape of a rectangular parallelepiped. All parts of the microorganism cultivation vial are made of polypropylene, the highest grade, polystyrene or styrene copolymer. A glidant additive is introduced into the main material in an amount of 0.2-0.5% of the total polymer mass to facilitate the movement of parts relative to each other. A useful model allows expanding the possibilities of cultivating slowly growing microorganisms on lamellar agar, preventing premature dehydration of the medium and providing access to selection colonies without the need to fully open the lid.

Description

The utility model relates to the field of medicine and biotechnology and can be used for the cultivation of rapidly and slowly growing microorganisms in solid nutrient media.

New identification methods allow the cultivation of slowly growing microorganisms (non-tuberculous mycobacteria, nocardia, rhodococci) in modern microbiological laboratories. However, the cultivation of such microorganisms using conventional Petri dishes and test tubes significantly limits the possibilities of working with them, because in this case, a sufficiently rapid dehydration of the medium occurs, which can significantly affect its growth qualities. In vitro cultivation virtually eliminates the isolation of a pure culture of microorganisms in the initial culture, or requires laborious procedures for diluting the primary material.

It should also be noted that some microorganisms (non-tuberculous mycobacteria, nocardia, rhodococcus, fungi) grow on solid nutrient media in the form of R-colonies with partial growth into the medium. The use of standard Petri dishes for cultivation significantly complicates the work with them due to the inability to use both hands to a doctor or laboratory assistant for the selection of colonies, because the lid of the Petri dish should be fixed in the hand above the bottom to reduce the risk of contamination.

Thus, it is necessary to create conditions that allow expanding the possibilities of cultivation of slowly growing microorganisms on plate agar without premature dehydration of the medium

The purpose of creating a useful model is to develop a bottle for the cultivation of microorganisms, which allows you to expand the possibilities of cultivating slowly growing microorganisms on lamellar agar without premature dehydration of the medium. This goal is achieved as follows.

Brief description of the drawing. On the drawings attached to the description is a diagram of the design of the bottle for the cultivation of microorganisms.

Implementation of a utility model. The proposed version of the bottle for the cultivation of microorganisms consists of two parts (cup and body). The case (figure No. 1) is a rectangular parallelepiped with a through hole of rectangular cross section and has the following geometric parameters: 86.96-87.04 × 86.96-87.04 × 42.94 mm. The through hole of a rectangular section has the following geometric parameters: 81.05-81.1 × 37.05-37.1 mm. The thickness of the side walls is 2.93 mm. The thickness of the upper wall is from 1.86 mm to 1.95 mm. The thickness of the bottom wall is from 1.98 mm to 2 mm. On the upper and lower walls there are sides for stacking bottles with a radius of 1.5 mm. The cup (figure No. 2) has the shape of a rectangular parallelepiped. Geometrical parameters of the cup: 81.22-81.3 × 81.22-81.3 × 37.22-37.3 mm. The wall thickness is 1.5 mm. The radii at the junctions of faces and edges are 0.6 mm.

All parts of the bottle for the cultivation of microorganisms are made of polypropylene 21060-16 high grade GOST 26996-86, polystyrene GOST 20281-86 or styrene copolymer MSN-L GOST 12271-76. LOXAMID OA SPEZIAL sliding additive is introduced into the main material in an amount of 0.2-0.5% of the total polymer mass to facilitate the movement of parts relative to each other.

The utility model allows expanding the possibilities of cultivation of slowly growing microorganisms on lamellar agar without premature dehydration of the medium.

INFORMATION SOURCES:

1. Clinical laboratory diagnostics: national leadership: in 2 volumes - T.P. / Ed. V.V. Dolgova, V.V. Menshikov. - M.: GEOTAR-Media, 2013 .-- 808 p.

2. Guide to medical microbiology. General and sanitary microbiology. Book I / Coll. authors // Ed. A.S. Labinskaya, E.G. Volina. - M.: Publishing house BINOM, 2008. - 1080 p.

3. Methods of general bacterology: Per. from English / Ed. F. Gerhardt et al. - Moscow: Mir, 1983 .-- 536 p.

Claims (1)

A bottle for the cultivation of microorganisms, characterized in that it consists of a cup and a body, where the body is a rectangular parallelepiped with a through hole of rectangular cross section in size corresponding to the cup that is placed in the body, on the upper and lower walls of the body there are bumpers with a radius of 1, 5 mm, the cup is also a rectangular parallelepiped, while all parts of the bottle are made of the highest grade of polypropylene, polystyrene or styrene copolymer, the base material is also introduced a sliding additive in an amount of 0.2-0.5% of the total polymer mass to facilitate the movement of the cup.

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