RO84708B - Procedeu pentru prepararea 5'-ribonucleotidelor - Google Patents

Procedeu pentru prepararea 5'-ribonucleotidelor

Info

Publication number
RO84708B
RO84708B RO108636A RO10863682A RO84708B RO 84708 B RO84708 B RO 84708B RO 108636 A RO108636 A RO 108636A RO 10863682 A RO10863682 A RO 10863682A RO 84708 B RO84708 B RO 84708B
Authority
RO
Romania
Prior art keywords
mixture
crude
ribonucleotides
phosphodiesterase
methacrylamide
Prior art date
Application number
RO108636A
Other languages
English (en)
Other versions
RO84708A (ro
Inventor
Reinhold Keller
Merten Schlingmann
Original Assignee
Hoechst Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Aktiengesellschaft filed Critical Hoechst Aktiengesellschaft
Publication of RO84708A publication Critical patent/RO84708A/ro
Publication of RO84708B publication Critical patent/RO84708B/ro

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C12N11/12—Cellulose or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Inventia se refera la un procedeu pentru prepararea 5'-ribonucleotitelor din acizi nucleici cu 5'-fosfodiesteraza constînd din aceea cî acizii ribonucleici dintr-o solutie de acid nucleic brut care contine acidul dezoxibonucleic cu greutate moleculara mai mare de 200.000 si acid ribonucleic cu greutati moleculare de 10.000 pîna la 50.000, obtinuta din microorganisme prin extractia lipidelor cu amoniac si alcanoli inferiori urmata de extractie apoasa se hidrolizeaza selectiv prin imobilizarea 5'-fosfodiesterazei pe un suport macroporos de copolimer format din metacrilat de glicil, eter alilglicidil, metacrilamida cu canale cu diametrul de maximum 100 nm si un volum al porilor de 2-3 ml/g dupa care 5'-ribonucleotidele prin metode în sine cunoscute.
RO108636A 1981-09-17 1982-09-15 Procedeu pentru prepararea 5'-ribonucleotidelor RO84708B (ro)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19813136940 DE3136940A1 (de) 1981-09-17 1981-09-17 "verfahren zur herstellung von 5'-ribonucleotiden"

Publications (2)

Publication Number Publication Date
RO84708A RO84708A (ro) 1984-07-17
RO84708B true RO84708B (ro) 1984-09-30

Family

ID=6141916

Family Applications (1)

Application Number Title Priority Date Filing Date
RO108636A RO84708B (ro) 1981-09-17 1982-09-15 Procedeu pentru prepararea 5'-ribonucleotidelor

Country Status (12)

Country Link
US (1) US4649111A (ro)
EP (1) EP0075262B1 (ro)
JP (1) JPS5867192A (ro)
KR (1) KR840001593A (ro)
AT (1) ATE24019T1 (ro)
AU (1) AU551838B2 (ro)
CA (1) CA1191102A (ro)
DE (2) DE3136940A1 (ro)
ES (1) ES8306163A1 (ro)
RO (1) RO84708B (ro)
SU (1) SU1435158A3 (ro)
ZA (1) ZA826796B (ro)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL90600A0 (en) * 1988-06-16 1990-01-18 Du Pont Polynucleotide phosphorylase immobilized on epoxy-activated beads
WO1991000346A1 (en) * 1989-07-03 1991-01-10 E.I. Du Pont De Nemours And Company Polynucleotide phosphorylase immobilized on tris(hydroxymethyl)methylacrylamide polymer beads
US6277562B1 (en) * 1993-01-26 2001-08-21 Robert-A. Ollar Method for paraffinophilic isolation and identification from a body specimen
EP1158047B1 (en) * 1999-03-05 2009-05-20 Mitsubishi Rayon Co., Ltd. Microarrays having biological substance
DE602004025199D1 (de) * 2003-01-27 2010-03-11 Dsm Ip Assets Bv Herstellung von 5'-ribonukleotiden
WO2004078342A1 (ja) 2003-03-04 2004-09-16 Manac Inc. アニオン性置換基を有する物質の捕捉剤
AU2003902303A0 (en) * 2003-05-14 2003-05-29 Protech Research Pty Ltd Extracting and purifying enzymes
US20100056671A1 (en) * 2007-04-12 2010-03-04 Designer Molecules, Inc. Polyfunctional epoxy oligomers
ITUA20161550A1 (it) * 2016-03-10 2017-09-10 Prosol S P A Procedimento per la produzione di RNA
EP4166665A1 (en) * 2021-10-12 2023-04-19 Prosol S.p.A. Process for the production of rna and 5'-ribonucleotides with a high degree of purity

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3139385A (en) * 1960-12-20 1964-06-30 Takeda Chemical Industries Ltd Method for the production of 5'-nucleotides
GB940660A (en) * 1961-02-04 1963-10-30 Waldhof Zellstoff Fab Improvements in and relating to the production of 5-nucleotides
US4044196A (en) * 1972-03-30 1977-08-23 Bayer Aktiengesellschaft Crosslinked copolymers of α,β-olefinically unsaturated dicarboxylic anhydrides
DE2215539C2 (de) * 1972-03-30 1984-08-02 Bayer Ag, 5090 Leverkusen Neue wasserunlösliche Enzym-, insbesondere Penicillinacylase-oder Enzyminhibitor-Präparate
FR2308636A1 (fr) * 1975-04-23 1976-11-19 Choay Sa Matrices portant simultanement plusieurs fonctions enzymatiques differentes, leur procede de production, et procede de preparation d'oligonucleotides et de polynucleotides a terminaisons specifiques, a l'aide desdites matrices
US4206243A (en) * 1976-07-27 1980-06-03 Hoechst Aktiengesellschaft Process for reducing the contents of lipids and nucleic acid in microbial cell masses
US4208309A (en) * 1977-05-20 1980-06-17 Rohm Gmbh Pearl polymer containing hollow pearls
DE2722751C2 (de) * 1977-05-20 1990-06-21 Röhm GmbH, 6100 Darmstadt Perlpolymerisat mit einem Teilchendurchmesser von 5 bis 1000 μm und innerem Hohlraum
US4342833A (en) * 1977-05-31 1982-08-03 Bethesda Research Laboratory Immobilized restriction endonucleases
DE2732301C3 (de) * 1977-07-16 1980-12-11 Roehm Gmbh, 6100 Darmstadt Verfahren zur Herstellung von stabilisierten trägergebundenen Proteinen
JPS5592672A (en) * 1979-01-05 1980-07-14 Ajinomoto Co Inc Preparation of yeast extract

Also Published As

Publication number Publication date
ES515775A0 (es) 1983-05-01
KR840001593A (ko) 1984-05-07
CA1191102A (en) 1985-07-30
EP0075262A2 (de) 1983-03-30
US4649111A (en) 1987-03-10
AU8844782A (en) 1983-03-24
EP0075262A3 (en) 1984-10-10
JPS5867192A (ja) 1983-04-21
ATE24019T1 (de) 1986-12-15
ZA826796B (en) 1983-07-27
ES8306163A1 (es) 1983-05-01
DE3136940A1 (de) 1983-03-31
AU551838B2 (en) 1986-05-15
SU1435158A3 (ru) 1988-10-30
DE3274560D1 (en) 1987-01-15
RO84708A (ro) 1984-07-17
EP0075262B1 (de) 1986-12-03

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