RO84708B - Procedeu pentru prepararea 5'-ribonucleotidelor - Google Patents
Procedeu pentru prepararea 5'-ribonucleotidelorInfo
- Publication number
- RO84708B RO84708B RO108636A RO10863682A RO84708B RO 84708 B RO84708 B RO 84708B RO 108636 A RO108636 A RO 108636A RO 10863682 A RO10863682 A RO 10863682A RO 84708 B RO84708 B RO 84708B
- Authority
- RO
- Romania
- Prior art keywords
- mixture
- crude
- ribonucleotides
- phosphodiesterase
- methacrylamide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/087—Acrylic polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Inventia se refera la un procedeu pentru prepararea 5'-ribonucleotitelor din acizi nucleici cu 5'-fosfodiesteraza constînd din aceea cî acizii ribonucleici dintr-o solutie de acid nucleic brut care contine acidul dezoxibonucleic cu greutate moleculara mai mare de 200.000 si acid ribonucleic cu greutati moleculare de 10.000 pîna la 50.000, obtinuta din microorganisme prin extractia lipidelor cu amoniac si alcanoli inferiori urmata de extractie apoasa se hidrolizeaza selectiv prin imobilizarea 5'-fosfodiesterazei pe un suport macroporos de copolimer format din metacrilat de glicil, eter alilglicidil, metacrilamida cu canale cu diametrul de maximum 100 nm si un volum al porilor de 2-3 ml/g dupa care 5'-ribonucleotidele prin metode în sine cunoscute.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19813136940 DE3136940A1 (de) | 1981-09-17 | 1981-09-17 | "verfahren zur herstellung von 5'-ribonucleotiden" |
Publications (2)
Publication Number | Publication Date |
---|---|
RO84708A RO84708A (ro) | 1984-07-17 |
RO84708B true RO84708B (ro) | 1984-09-30 |
Family
ID=6141916
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RO108636A RO84708B (ro) | 1981-09-17 | 1982-09-15 | Procedeu pentru prepararea 5'-ribonucleotidelor |
Country Status (12)
Country | Link |
---|---|
US (1) | US4649111A (ro) |
EP (1) | EP0075262B1 (ro) |
JP (1) | JPS5867192A (ro) |
KR (1) | KR840001593A (ro) |
AT (1) | ATE24019T1 (ro) |
AU (1) | AU551838B2 (ro) |
CA (1) | CA1191102A (ro) |
DE (2) | DE3136940A1 (ro) |
ES (1) | ES515775A0 (ro) |
RO (1) | RO84708B (ro) |
SU (1) | SU1435158A3 (ro) |
ZA (1) | ZA826796B (ro) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL90600A0 (en) * | 1988-06-16 | 1990-01-18 | Du Pont | Polynucleotide phosphorylase immobilized on epoxy-activated beads |
AU5943690A (en) * | 1989-07-03 | 1991-01-17 | E.I. Du Pont De Nemours And Company | Polynucleotide phosphorylase immobilized on tris(hydroxymethyl)methylacrylamide polymer beads |
US6277562B1 (en) * | 1993-01-26 | 2001-08-21 | Robert-A. Ollar | Method for paraffinophilic isolation and identification from a body specimen |
KR100538502B1 (ko) * | 1999-03-05 | 2005-12-23 | 미쯔비시 레이온 가부시끼가이샤 | 생체 관련 물질 함유 담체 |
ATE455859T1 (de) * | 2003-01-27 | 2010-02-15 | Dsm Ip Assets Bv | Herstellung von 5'-ribonukleotiden |
WO2004078342A1 (ja) | 2003-03-04 | 2004-09-16 | Manac Inc. | アニオン性置換基を有する物質の捕捉剤 |
AU2003902303A0 (en) * | 2003-05-14 | 2003-05-29 | Protech Research Pty Ltd | Extracting and purifying enzymes |
WO2008128209A1 (en) * | 2007-04-12 | 2008-10-23 | Designer Molecules, Inc. | Polyfunctional epoxy oligomers |
ITUA20161550A1 (it) * | 2016-03-10 | 2017-09-10 | Prosol S P A | Procedimento per la produzione di RNA |
EP4166665A1 (en) * | 2021-10-12 | 2023-04-19 | Prosol S.p.A. | Process for the production of rna and 5'-ribonucleotides with a high degree of purity |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3139385A (en) * | 1960-12-20 | 1964-06-30 | Takeda Chemical Industries Ltd | Method for the production of 5'-nucleotides |
GB940660A (en) * | 1961-02-04 | 1963-10-30 | Waldhof Zellstoff Fab | Improvements in and relating to the production of 5-nucleotides |
US4044196A (en) * | 1972-03-30 | 1977-08-23 | Bayer Aktiengesellschaft | Crosslinked copolymers of α,β-olefinically unsaturated dicarboxylic anhydrides |
DE2215539C2 (de) * | 1972-03-30 | 1984-08-02 | Bayer Ag, 5090 Leverkusen | Neue wasserunlösliche Enzym-, insbesondere Penicillinacylase-oder Enzyminhibitor-Präparate |
FR2308636A1 (fr) * | 1975-04-23 | 1976-11-19 | Choay Sa | Matrices portant simultanement plusieurs fonctions enzymatiques differentes, leur procede de production, et procede de preparation d'oligonucleotides et de polynucleotides a terminaisons specifiques, a l'aide desdites matrices |
JPS5218892A (en) * | 1975-08-04 | 1977-02-12 | Kyowa Hakko Kogyo Co Ltd | Process for preparing 5'-ribonucleotides |
US4206243A (en) * | 1976-07-27 | 1980-06-03 | Hoechst Aktiengesellschaft | Process for reducing the contents of lipids and nucleic acid in microbial cell masses |
US4208309A (en) * | 1977-05-20 | 1980-06-17 | Rohm Gmbh | Pearl polymer containing hollow pearls |
DE2722751C2 (de) * | 1977-05-20 | 1990-06-21 | Röhm GmbH, 6100 Darmstadt | Perlpolymerisat mit einem Teilchendurchmesser von 5 bis 1000 μm und innerem Hohlraum |
US4342833A (en) * | 1977-05-31 | 1982-08-03 | Bethesda Research Laboratory | Immobilized restriction endonucleases |
DE2732301C3 (de) * | 1977-07-16 | 1980-12-11 | Roehm Gmbh, 6100 Darmstadt | Verfahren zur Herstellung von stabilisierten trägergebundenen Proteinen |
JPS5592672A (en) * | 1979-01-05 | 1980-07-14 | Ajinomoto Co Inc | Preparation of yeast extract |
-
1981
- 1981-09-17 DE DE19813136940 patent/DE3136940A1/de not_active Withdrawn
-
1982
- 1982-09-15 DE DE8282108494T patent/DE3274560D1/de not_active Expired
- 1982-09-15 RO RO108636A patent/RO84708B/ro unknown
- 1982-09-15 EP EP82108494A patent/EP0075262B1/de not_active Expired
- 1982-09-15 AT AT82108494T patent/ATE24019T1/de not_active IP Right Cessation
- 1982-09-15 KR KR1019820004171A patent/KR840001593A/ko unknown
- 1982-09-16 CA CA000411629A patent/CA1191102A/en not_active Expired
- 1982-09-16 AU AU88447/82A patent/AU551838B2/en not_active Ceased
- 1982-09-16 SU SU823494005A patent/SU1435158A3/ru active
- 1982-09-16 JP JP57159744A patent/JPS5867192A/ja active Pending
- 1982-09-16 ZA ZA826796A patent/ZA826796B/xx unknown
- 1982-09-17 ES ES515775A patent/ES515775A0/es active Granted
-
1985
- 1985-08-12 US US06/764,967 patent/US4649111A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA1191102A (en) | 1985-07-30 |
DE3274560D1 (en) | 1987-01-15 |
DE3136940A1 (de) | 1983-03-31 |
US4649111A (en) | 1987-03-10 |
ATE24019T1 (de) | 1986-12-15 |
RO84708A (ro) | 1984-07-17 |
EP0075262B1 (de) | 1986-12-03 |
ZA826796B (en) | 1983-07-27 |
AU551838B2 (en) | 1986-05-15 |
KR840001593A (ko) | 1984-05-07 |
AU8844782A (en) | 1983-03-24 |
ES8306163A1 (es) | 1983-05-01 |
EP0075262A2 (de) | 1983-03-30 |
JPS5867192A (ja) | 1983-04-21 |
SU1435158A3 (ru) | 1988-10-30 |
ES515775A0 (es) | 1983-05-01 |
EP0075262A3 (en) | 1984-10-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JPS57115186A (en) | Preparation of l-lysine by fermentation | |
RO84708B (ro) | Procedeu pentru prepararea 5'-ribonucleotidelor | |
DE69734901T2 (de) | Verfahren zur Herstellung von Carbonsäuren | |
EP0071023A3 (en) | Method for producing l-isoleucine by fermentation | |
ES8503692A1 (es) | Metodo de fabricacion de polivinilbutirol para vidrieras intercaladas. | |
US5077212A (en) | Process for the production of acylamide amidohydrolase by methylophilus methylotrophus | |
EP0464325A3 (en) | Process for the preparation of l-ornithine salts | |
JPS55144889A (en) | Continuous preparation of acrylamide or methacrylamide by microorganism | |
GB1377313A (en) | Process for purifying phenylalanine derivatives | |
ES8607978A1 (es) | Un procedimiento para preparar alcoholes manosacaridos | |
EP0272026A2 (en) | Process for the decomposition of acrylamide | |
JPS561888A (en) | Preparation of concentrated aqueous solution of acrylamide with microorganism | |
DE3576522D1 (de) | Verfahren zur enzymatischen hydrolyse von d-alpha-aminosaeureamiden. | |
Keller et al. | Process for the preparation of 5-ribonucleotides. | |
ATE35826T1 (de) | Verfahren zur herstellung von l-alphaaminos|uren. | |
Chibata et al. | Continuous enzyme reactions by immobilized microbial cells | |
JPS55108294A (en) | Production of purine nucleoside through fermentation process | |
FR2313394A1 (fr) | Procede de preparation d'un produit doue d'activite enzymatique | |
Walker | [24] ATP: Inosamine phosphotransferase (s) | |
JPS57163487A (en) | Production of l-arginine through fermentation process | |
GB1209711A (en) | A process for production of inosine and 5'-guanylic acid nucleotides | |
GB1469358A (en) | Enzyme product | |
JPS57115185A (en) | Preparation of l-lysine by fermentation | |
JPS57138386A (en) | Aqueous nutrition culture medium for culturing microorganism | |
ATE56955T1 (de) | Verfahren fuer (1h>-isoindolin-1-on-3-carbonsaeure. |