PT3004378T - Resumo - Google Patents
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- PT3004378T PT3004378T PT147254981T PT14725498T PT3004378T PT 3004378 T PT3004378 T PT 3004378T PT 147254981 T PT147254981 T PT 147254981T PT 14725498 T PT14725498 T PT 14725498T PT 3004378 T PT3004378 T PT 3004378T
- Authority
- PT
- Portugal
- Prior art keywords
- polymerase
- nucleic acid
- nanopore
- nucleotide
- pyrophosphorolysis
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
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- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Kinetics & Catalysis (AREA)
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| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| EP3004378B1 (en) * | 2013-05-24 | 2017-12-20 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing using nanopores |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| CN119899249A (zh) | 2014-09-01 | 2025-04-29 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| KR102222191B1 (ko) | 2016-03-02 | 2021-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 기공 |
| CN118272512A (zh) | 2016-04-06 | 2024-07-02 | 牛津纳米孔科技公开有限公司 | 突变体孔 |
| WO2018195222A1 (en) * | 2017-04-19 | 2018-10-25 | Electronic Biosciences, Inc. | Nanopore/nanowell electrode enabled exonuclease sequencing |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| EP3645552B1 (en) * | 2017-06-30 | 2023-06-28 | Vib Vzw | Novel protein pores |
| JP7499761B2 (ja) | 2018-11-08 | 2024-06-14 | オックスフォード ナノポール テクノロジーズ ピーエルシー | 細孔 |
| GB201818216D0 (en) | 2018-11-08 | 2018-12-26 | Oxford Nanopore Tech Ltd | Pore |
| CN113677693B (zh) | 2019-04-09 | 2025-10-21 | 牛津纳米孔科技公开有限公司 | 孔 |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| GB201919186D0 (en) | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Simplified polynucleotide sequence detection method |
| IL293936A (en) * | 2019-12-23 | 2022-08-01 | Biofidelity Ltd | kits and devices |
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| EP4168582B1 (en) | 2020-06-18 | 2025-11-19 | Oxford Nanopore Technologies plc | A method of selectively characterising a polynucleotide using a detector |
| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| GB202118939D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Plc | Pore |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
| AU2023322679A1 (en) | 2022-08-09 | 2025-01-23 | Oxford Nanopore Technologies Plc | De novo pores |
| GB202211607D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| CA3262948A1 (en) | 2022-08-09 | 2024-02-15 | Oxford Nanopore Technologies Plc | NEW PORE-FORMING MONOMERS AND PORES |
| GB202211602D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| CN120303290A (zh) | 2022-10-28 | 2025-07-11 | 牛津纳米孔科技公开有限公司 | 孔单体和孔 |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
| WO2025006749A1 (en) * | 2023-06-30 | 2025-01-02 | Illumina, Inc. | Using apertures to capture polynucleotides on particles |
| GB202315935D0 (en) | 2023-10-18 | 2023-11-29 | Oxford Nanopore Tech Ltd | Novel enzymes |
| GB202405960D0 (en) | 2024-04-29 | 2024-06-12 | Oxford Nanopore Tech Plc | Photo-active conjugates and their uses |
| GB202413506D0 (en) | 2024-09-13 | 2024-10-30 | Oxford Nanopore Tech Plc | Pore monomers and their uses |
| WO2026120129A1 (en) | 2024-12-06 | 2026-06-11 | Oxford Nanopore Technologies plc. | Pore constructs and their uses |
| GB202500688D0 (en) | 2025-01-17 | 2025-03-05 | Oxford Nanopore Tech Plc | Pore monomers and their uses |
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| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US6107023A (en) | 1988-06-17 | 2000-08-22 | Genelabs Technologies, Inc. | DNA amplification and subtraction techniques |
| US5498523A (en) | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| US4962020A (en) | 1988-07-12 | 1990-10-09 | President And Fellows Of Harvard College | DNA sequencing |
| KR920700294A (ko) * | 1989-04-12 | 1992-02-19 | 원본미기재 | 개선된 프라이머 연장반응 |
| US6268146B1 (en) * | 1998-03-13 | 2001-07-31 | Promega Corporation | Analytical methods and materials for nucleic acid detection |
| US7090975B2 (en) | 1998-03-13 | 2006-08-15 | Promega Corporation | Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection |
| US6159693A (en) * | 1998-03-13 | 2000-12-12 | Promega Corporation | Nucleic acid detection |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| EP1192103A1 (en) | 1999-06-22 | 2002-04-03 | President And Fellows of Harvard College | Control of solid state dimensional features |
| US7582490B2 (en) | 1999-06-22 | 2009-09-01 | President And Fellows Of Harvard College | Controlled fabrication of gaps in electrically conducting structures |
| US6916665B2 (en) | 2000-02-11 | 2005-07-12 | The Texas A&M University System | Biosensor compositions and methods of use |
| US7033763B2 (en) | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| WO2003080861A1 (en) * | 2002-03-22 | 2003-10-02 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Single molecule sequencing using phosphate labeled nucleotides |
| US7444053B2 (en) | 2003-06-16 | 2008-10-28 | The Regents Of The University Of California | Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity |
| WO2005082922A1 (en) * | 2004-02-26 | 2005-09-09 | Layerlab Aktiebolag | Oligonucleotides related to lipid membrane attachments |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US7867716B2 (en) | 2004-12-21 | 2011-01-11 | The Texas A&M University System | High temperature ion channels and pores |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| GB0523282D0 (en) * | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| JP5063616B2 (ja) * | 2006-02-03 | 2012-10-31 | インテジェニックス インコーポレイテッド | マイクロ流体デバイス |
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| GB0717150D0 (en) | 2007-09-04 | 2007-10-17 | Univ Warwick | Apparatus and method |
| WO2009068268A1 (en) | 2007-11-28 | 2009-06-04 | Roche Diagnostics Gmbh | Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability |
| US20110229877A1 (en) | 2008-07-07 | 2011-09-22 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| DE202009019021U1 (de) | 2008-09-22 | 2015-09-24 | University Of Washington | Msp-nanoporen |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
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| GB201300957D0 (en) | 2013-01-18 | 2013-03-06 | Base4 Innovation Ltd | Sequencing method |
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2014
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| CA2907423A1 (en) | 2014-11-27 |
| EP3351645A1 (en) | 2018-07-25 |
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| US20220177960A1 (en) | 2022-06-09 |
| EP3825415B1 (en) | 2024-08-28 |
| WO2014187924A1 (en) | 2014-11-27 |
| EP3004378B1 (en) | 2017-12-20 |
| EP3004378A1 (en) | 2016-04-13 |
| DK3004378T3 (en) | 2018-03-26 |
| US20140349300A1 (en) | 2014-11-27 |
| US11286521B2 (en) | 2022-03-29 |
| US10450605B2 (en) | 2019-10-22 |
| CN105264093B (zh) | 2018-04-10 |
| JP6329624B2 (ja) | 2018-05-23 |
| US20200157619A1 (en) | 2020-05-21 |
| US20180105874A1 (en) | 2018-04-19 |
| CN105264093A (zh) | 2016-01-20 |
| AU2014270410A1 (en) | 2015-10-08 |
| ES2994071T3 (en) | 2025-01-16 |
| ES2817425T3 (es) | 2021-04-07 |
| ES2660671T3 (es) | 2018-03-23 |
| AU2014270410B2 (en) | 2020-07-16 |
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