JP6329624B2 - 加ピロリン酸分解式配列決定法 - Google Patents

加ピロリン酸分解式配列決定法 Download PDF

Info

Publication number
JP6329624B2
JP6329624B2 JP2016514422A JP2016514422A JP6329624B2 JP 6329624 B2 JP6329624 B2 JP 6329624B2 JP 2016514422 A JP2016514422 A JP 2016514422A JP 2016514422 A JP2016514422 A JP 2016514422A JP 6329624 B2 JP6329624 B2 JP 6329624B2
Authority
JP
Japan
Prior art keywords
polymerase
nucleic acid
nanopore
pyrophosphate
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2016514422A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016526876A (ja
JP2016526876A5 (enExample
Inventor
ムールマン ウォウター
ムールマン ウォウター
Original Assignee
イルミナ ケンブリッジ リミテッド
イルミナ ケンブリッジ リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イルミナ ケンブリッジ リミテッド, イルミナ ケンブリッジ リミテッド filed Critical イルミナ ケンブリッジ リミテッド
Publication of JP2016526876A publication Critical patent/JP2016526876A/ja
Publication of JP2016526876A5 publication Critical patent/JP2016526876A5/ja
Application granted granted Critical
Publication of JP6329624B2 publication Critical patent/JP6329624B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2016514422A 2013-05-24 2014-05-22 加ピロリン酸分解式配列決定法 Expired - Fee Related JP6329624B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361827175P 2013-05-24 2013-05-24
US61/827,175 2013-05-24
PCT/EP2014/060588 WO2014187924A1 (en) 2013-05-24 2014-05-22 Pyrophosphorolytic sequencing

Publications (3)

Publication Number Publication Date
JP2016526876A JP2016526876A (ja) 2016-09-08
JP2016526876A5 JP2016526876A5 (enExample) 2017-06-22
JP6329624B2 true JP6329624B2 (ja) 2018-05-23

Family

ID=50771301

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016514422A Expired - Fee Related JP6329624B2 (ja) 2013-05-24 2014-05-22 加ピロリン酸分解式配列決定法

Country Status (10)

Country Link
US (4) US20140349300A1 (enExample)
EP (3) EP3004378B1 (enExample)
JP (1) JP6329624B2 (enExample)
CN (1) CN105264093B (enExample)
AU (1) AU2014270410B2 (enExample)
CA (1) CA2907423A1 (enExample)
DK (2) DK3351645T3 (enExample)
ES (3) ES2994071T3 (enExample)
PT (1) PT3004378T (enExample)
WO (1) WO2014187924A1 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
BR112015021788B1 (pt) 2013-03-08 2023-02-28 Oxford Nanopore Technologies Plc Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
ES2994071T3 (en) * 2013-05-24 2025-01-16 Illumina Cambridge Ltd Pyrophosphorolytic sequencing using nanopores
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
EP3137627B1 (en) 2014-05-02 2025-07-30 Oxford Nanopore Technologies plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
EP4122949B1 (en) 2016-04-06 2024-06-05 Oxford Nanopore Technologies plc Mutant pore
EP4026916B1 (en) * 2017-04-19 2023-10-18 Electronic Biosciences Inc. Nanopore/nanowell electrode enabled exonuclease sequencing
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
EP3877547A1 (en) 2018-11-08 2021-09-15 Oxford Nanopore Technologies Limited Pore
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
JP2022529623A (ja) 2019-04-09 2022-06-23 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
CA3165865A1 (en) * 2019-12-23 2021-07-01 Biofidelity Ltd Kits and devices
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
GB202118939D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Plc Pore
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
CN119678047A (zh) 2022-08-09 2025-03-21 牛津纳米孔科技公开有限公司 从头孔
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
CA3262948A1 (en) 2022-08-09 2024-02-15 The Regents Of The University Of California NEW PORE-FORMING MONOMERS AND PORES
JP2026512988A (ja) 2022-10-28 2026-04-22 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔モノマーおよび細孔
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
US20250002985A1 (en) * 2023-06-30 2025-01-02 Illumina, Inc. Using apertures to capture polynucleotides on particles
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202413506D0 (en) 2024-09-13 2024-10-30 Oxford Nanopore Tech Plc Pore monomers and their uses

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
CA2050276C (en) * 1989-04-12 2003-03-11 Stanley Tabor Improved primer extension reactions
US6268146B1 (en) * 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6159693A (en) * 1998-03-13 2000-12-12 Promega Corporation Nucleic acid detection
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US7582490B2 (en) 1999-06-22 2009-09-01 President And Fellows Of Harvard College Controlled fabrication of gaps in electrically conducting structures
AU5631200A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Control of solid state dimensional features
EP2083015B1 (en) 2000-02-11 2016-04-06 The Texas A & M University System Biosensor compositions and methods of use
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
US7444053B2 (en) 2003-06-16 2008-10-28 The Regents Of The University Of California Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity
US8389707B2 (en) * 2004-02-26 2013-03-05 Bio-Rad Laboratories Inc. Oligonucleotides related to lipid membrane attachments
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2007084103A2 (en) 2004-12-21 2007-07-26 The Texas A & M University System High temperature ion channels and pores
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2008030631A2 (en) * 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
US20100196203A1 (en) 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
GB0717150D0 (en) 2007-09-04 2007-10-17 Univ Warwick Apparatus and method
ES2553893T3 (es) 2007-11-28 2015-12-14 F. Hoffmann-La Roche Ag ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
EP2675913B1 (en) * 2011-02-15 2016-12-14 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
EP3633370B1 (en) * 2011-05-27 2024-05-01 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
CN104011866B (zh) * 2011-07-27 2017-06-09 伊利诺伊大学评议会 用于生物分子表征的纳米孔传感器
US9279154B2 (en) * 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
GB201300957D0 (en) 2013-01-18 2013-03-06 Base4 Innovation Ltd Sequencing method
ES2994071T3 (en) * 2013-05-24 2025-01-16 Illumina Cambridge Ltd Pyrophosphorolytic sequencing using nanopores

Also Published As

Publication number Publication date
EP3825415B1 (en) 2024-08-28
HK1261919A1 (en) 2020-01-10
ES2994071T3 (en) 2025-01-16
US20220177960A1 (en) 2022-06-09
ES2660671T3 (es) 2018-03-23
ES2817425T3 (es) 2021-04-07
US20200157619A1 (en) 2020-05-21
JP2016526876A (ja) 2016-09-08
EP3351645A1 (en) 2018-07-25
EP3351645B1 (en) 2020-07-15
US11286521B2 (en) 2022-03-29
US10450605B2 (en) 2019-10-22
DK3351645T3 (da) 2020-09-21
CA2907423A1 (en) 2014-11-27
EP3004378A1 (en) 2016-04-13
US20140349300A1 (en) 2014-11-27
DK3004378T3 (en) 2018-03-26
PT3004378T (pt) 2018-03-06
EP3825415A1 (en) 2021-05-26
WO2014187924A1 (en) 2014-11-27
AU2014270410A1 (en) 2015-10-08
EP3004378B1 (en) 2017-12-20
AU2014270410B2 (en) 2020-07-16
US20180105874A1 (en) 2018-04-19
CN105264093B (zh) 2018-04-10
CN105264093A (zh) 2016-01-20

Similar Documents

Publication Publication Date Title
US20220177960A1 (en) Pyrophosphorolytic sequencing
JP7349972B2 (ja) ポリヌクレオチド配列決定のための組成物及び方法
US20200291452A1 (en) Method
CN109196116B (zh) 一种表征靶多核苷酸的方法
HK40047390A (en) Pyrophosphorolytic sequencing using nanopores
HK40047390B (en) Pyrophosphorolytic sequencing using nanopores
HK1261919B (en) Pyrophosphorolytic sequencing using nanopores

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20151228

RD01 Notification of change of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7426

Effective date: 20151228

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170509

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20170509

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20180322

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180403

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180420

R150 Certificate of patent or registration of utility model

Ref document number: 6329624

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees