CA2907423A1 - Pyrophosphorolytic sequencing - Google Patents

Pyrophosphorolytic sequencing

Info

Publication number
CA2907423A1
CA2907423A1 CA2907423A CA2907423A CA2907423A1 CA 2907423 A1 CA2907423 A1 CA 2907423A1 CA 2907423 A CA2907423 A CA 2907423A CA 2907423 A CA2907423 A CA 2907423A CA 2907423 A1 CA2907423 A1 CA 2907423A1
Authority
CA
Canada
Prior art keywords
nucleic acid
nanopore
polymerase
target nucleic
pyrophosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2907423A
Other languages
English (en)
French (fr)
Inventor
Wouter Meuleman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Cambridge Ltd
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of CA2907423A1 publication Critical patent/CA2907423A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2907423A 2013-05-24 2014-05-22 Pyrophosphorolytic sequencing Pending CA2907423A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361827175P 2013-05-24 2013-05-24
US61/827,175 2013-05-24
PCT/EP2014/060588 WO2014187924A1 (en) 2013-05-24 2014-05-22 Pyrophosphorolytic sequencing

Publications (1)

Publication Number Publication Date
CA2907423A1 true CA2907423A1 (en) 2014-11-27

Family

ID=50771301

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2907423A Pending CA2907423A1 (en) 2013-05-24 2014-05-22 Pyrophosphorolytic sequencing

Country Status (10)

Country Link
US (4) US20140349300A1 (enExample)
EP (3) EP3004378B1 (enExample)
JP (1) JP6329624B2 (enExample)
CN (1) CN105264093B (enExample)
AU (1) AU2014270410B2 (enExample)
CA (1) CA2907423A1 (enExample)
DK (2) DK3004378T3 (enExample)
ES (3) ES2817425T3 (enExample)
PT (1) PT3004378T (enExample)
WO (1) WO2014187924A1 (enExample)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CA2907423A1 (en) * 2013-05-24 2014-11-27 Illumina Cambridge Limited Pyrophosphorolytic sequencing
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
CA3020203A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
EP4026916B1 (en) * 2017-04-19 2023-10-18 Electronic Biosciences Inc. Nanopore/nanowell electrode enabled exonuclease sequencing
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
CN113195736A (zh) 2018-11-08 2021-07-30 牛津纳米孔科技公司 孔
AU2020272997A1 (en) 2019-04-09 2021-10-21 Oxford Nanopore Technologies Plc Pore
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
EP4081660A1 (en) * 2019-12-23 2022-11-02 Biofidelity Ltd Kits and devices
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
GB202118939D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Plc Pore
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
EP4569331A1 (en) 2022-08-09 2025-06-18 Oxford Nanopore Technologies PLC De novo pores
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024033443A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
CN120303290A (zh) 2022-10-28 2025-07-11 牛津纳米孔科技公开有限公司 孔单体和孔
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
US20250002985A1 (en) * 2023-06-30 2025-01-02 Illumina, Inc. Using apertures to capture polynucleotides on particles
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
CA2050276C (en) * 1989-04-12 2003-03-11 Stanley Tabor Improved primer extension reactions
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6159693A (en) * 1998-03-13 2000-12-12 Promega Corporation Nucleic acid detection
US6268146B1 (en) * 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
AU5763000A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Molecular and atomic scale evaluation of biopolymers
US7582490B2 (en) 1999-06-22 2009-09-01 President And Fellows Of Harvard College Controlled fabrication of gaps in electrically conducting structures
US6916665B2 (en) 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
US7444053B2 (en) 2003-06-16 2008-10-28 The Regents Of The University Of California Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity
WO2005082922A1 (en) * 2004-02-26 2005-09-09 Layerlab Aktiebolag Oligonucleotides related to lipid membrane attachments
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0523282D0 (en) * 2005-11-15 2005-12-21 Isis Innovation Methods using pores
CA2641271A1 (en) * 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB0717150D0 (en) 2007-09-04 2007-10-17 Univ Warwick Apparatus and method
ES2553893T3 (es) 2007-11-28 2015-12-14 F. Hoffmann-La Roche Ag ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
ES2576114T3 (es) 2008-09-22 2016-07-05 University Of Washington Nanoporos MSP y procedimientos relacionados
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US20120208193A1 (en) * 2011-02-15 2012-08-16 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
EP3848706B1 (en) * 2011-05-27 2023-07-19 Oxford Nanopore Technologies PLC Coupling method
JP2014521956A (ja) * 2011-07-27 2014-08-28 ザ ボード オブ トラスティーズ オブ ザ ユニヴァーシティー オブ イリノイ 生体分子を特徴付けるためのナノ細孔センサー
US9279154B2 (en) * 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
GB201300957D0 (en) 2013-01-18 2013-03-06 Base4 Innovation Ltd Sequencing method
CA2907423A1 (en) * 2013-05-24 2014-11-27 Illumina Cambridge Limited Pyrophosphorolytic sequencing

Also Published As

Publication number Publication date
JP6329624B2 (ja) 2018-05-23
EP3351645A1 (en) 2018-07-25
CN105264093B (zh) 2018-04-10
EP3351645B1 (en) 2020-07-15
DK3004378T3 (en) 2018-03-26
ES2994071T3 (en) 2025-01-16
WO2014187924A1 (en) 2014-11-27
DK3351645T3 (da) 2020-09-21
AU2014270410B2 (en) 2020-07-16
PT3004378T (pt) 2018-03-06
AU2014270410A1 (en) 2015-10-08
CN105264093A (zh) 2016-01-20
US20200157619A1 (en) 2020-05-21
US20220177960A1 (en) 2022-06-09
EP3004378A1 (en) 2016-04-13
US20140349300A1 (en) 2014-11-27
EP3004378B1 (en) 2017-12-20
US10450605B2 (en) 2019-10-22
EP3825415A1 (en) 2021-05-26
US20180105874A1 (en) 2018-04-19
HK1261919A1 (en) 2020-01-10
ES2660671T3 (es) 2018-03-23
EP3825415B1 (en) 2024-08-28
ES2817425T3 (es) 2021-04-07
US11286521B2 (en) 2022-03-29
JP2016526876A (ja) 2016-09-08

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