PT2993231T - Estirpe bacteriana para expressão de proteínas recombinantes, apresentando degp deficiente em actividade de protease e retendo actividade chaperone, e genes tsp e ptr knocked out (inactivados) - Google Patents
Estirpe bacteriana para expressão de proteínas recombinantes, apresentando degp deficiente em actividade de protease e retendo actividade chaperone, e genes tsp e ptr knocked out (inactivados)Info
- Publication number
- PT2993231T PT2993231T PT15182886T PT15182886T PT2993231T PT 2993231 T PT2993231 T PT 2993231T PT 15182886 T PT15182886 T PT 15182886T PT 15182886 T PT15182886 T PT 15182886T PT 2993231 T PT2993231 T PT 2993231T
- Authority
- PT
- Portugal
- Prior art keywords
- bacterial strain
- protein expression
- recombinant protein
- knocked out
- chaperone activity
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/244—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0916821A GB0916821D0 (en) | 2009-09-24 | 2009-09-24 | Bacterial host strain |
| GBGB0916822.0A GB0916822D0 (en) | 2009-09-24 | 2009-09-24 | Bacterial host strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT2993231T true PT2993231T (pt) | 2018-11-12 |
Family
ID=43003459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT15182886T PT2993231T (pt) | 2009-09-24 | 2010-09-23 | Estirpe bacteriana para expressão de proteínas recombinantes, apresentando degp deficiente em actividade de protease e retendo actividade chaperone, e genes tsp e ptr knocked out (inactivados) |
Country Status (21)
| Country | Link |
|---|---|
| US (3) | US9109216B2 (enExample) |
| EP (2) | EP2480661A1 (enExample) |
| JP (2) | JP6132551B2 (enExample) |
| KR (1) | KR101758942B1 (enExample) |
| CN (1) | CN102575241B (enExample) |
| AU (2) | AU2010299640B2 (enExample) |
| BR (1) | BR112012006670B8 (enExample) |
| CA (1) | CA2774692C (enExample) |
| CY (1) | CY1120947T1 (enExample) |
| DK (1) | DK2993231T3 (enExample) |
| ES (1) | ES2692811T3 (enExample) |
| HR (1) | HRP20181482T1 (enExample) |
| HU (1) | HUE040306T2 (enExample) |
| IN (1) | IN2012DN02401A (enExample) |
| LT (1) | LT2993231T (enExample) |
| PL (1) | PL2993231T3 (enExample) |
| PT (1) | PT2993231T (enExample) |
| SG (1) | SG10201606462WA (enExample) |
| SI (1) | SI2993231T1 (enExample) |
| SM (1) | SMT201800549T1 (enExample) |
| WO (1) | WO2011036454A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN2012DN02401A (enExample) * | 2009-09-24 | 2015-08-21 | Ucb Pharma Sa | |
| GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201000591D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201012599D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Process for purifying proteins |
| PL2731973T3 (pl) * | 2011-07-13 | 2018-04-30 | Ucb Biopharma Sprl | Bakteryjny szczep gospodarz wykazujący ekspresję dsbc |
| GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
| EP3824906A1 (en) | 2016-12-21 | 2021-05-26 | Amgen Inc. | Anti-tnf alpha antibody formulations |
| EP3774926A1 (en) | 2018-04-05 | 2021-02-17 | Bio-Rad ABD Serotec GmbH | Display systems for proteins of interest |
| WO2020188350A1 (en) | 2019-03-18 | 2020-09-24 | Bio-Rad Abd Serotec Gmbh | Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation |
| CN110616181A (zh) * | 2019-09-27 | 2019-12-27 | 北京理工大学 | 一株基于大肠杆菌改造的工程菌株 |
| CN117965503B (zh) * | 2024-02-02 | 2024-08-09 | 南京迪诺薇华生物科技有限公司 | 一种蛋白酶突变体及其在洗护肤品、化妆品中的应用 |
Family Cites Families (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5264365A (en) | 1990-11-09 | 1993-11-23 | Board Of Regents, The University Of Texas System | Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides |
| US5508192A (en) | 1990-11-09 | 1996-04-16 | Board Of Regents, The University Of Texas System | Bacterial host strains for producing proteolytically sensitive polypeptides |
| GB9215540D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| US6027888A (en) | 1996-04-05 | 2000-02-22 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells |
| US6083715A (en) | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| JP4249832B2 (ja) * | 1998-12-28 | 2009-04-08 | タカラバイオ株式会社 | トリガーファクター発現プラスミド |
| AU767670B2 (en) * | 1999-06-29 | 2003-11-20 | Siga Technologies, Inc. | DEGP periplasmic protease a new anti-infective target and an invitro assay for DEGP protease function |
| GB0006398D0 (en) | 2000-03-16 | 2000-05-03 | Novartis Ag | Organic compounds |
| GB0013810D0 (en) | 2000-06-06 | 2000-07-26 | Celltech Chiroscience Ltd | Biological products |
| AU2001287040A1 (en) | 2000-09-01 | 2002-03-13 | Biogen, Inc. | Methods of designing and producing novel compounds having improved binding affinity for cd154 or other trimeric proteins |
| AU8867501A (en) | 2000-09-01 | 2002-03-13 | Biogen Inc | Co-crystal structure of monoclonal antibody 5c8 and cd154, and use thereof in drug design |
| US7041479B2 (en) | 2000-09-06 | 2006-05-09 | The Board Of Trustess Of The Leland Stanford Junior University | Enhanced in vitro synthesis of active proteins containing disulfide bonds |
| MXPA03005273A (es) | 2000-12-14 | 2003-09-25 | Genentech Inc | Anticuerpos producidos procarioticamente y sus usos. |
| CA2430249A1 (en) | 2000-12-14 | 2002-06-20 | Genentech, Inc. | Bacterial host strains |
| TWI334439B (en) | 2001-08-01 | 2010-12-11 | Centocor Inc | Anti-tnf antibodies, compositions, methods and uses |
| EP1427744B1 (en) | 2001-08-27 | 2007-12-26 | Genentech, Inc. | A system for antibody expression and assembly |
| GB0124317D0 (en) | 2001-10-10 | 2001-11-28 | Celltech R&D Ltd | Biological products |
| IL161988A0 (en) | 2001-11-16 | 2005-11-20 | Corporation Idec Pharmaceutica | Polycistronic expression of antibodies |
| GB0129105D0 (en) | 2001-12-05 | 2002-01-23 | Celltech R&D Ltd | Expression control using variable intergenic sequences |
| EP1517921B1 (en) | 2002-06-28 | 2006-06-07 | Domantis Limited | Dual specific ligands with increased serum half-life |
| AU2003287345A1 (en) | 2002-10-31 | 2004-06-07 | Genentech, Inc. | Methods and compositions for increasing antibody production |
| JP4603894B2 (ja) | 2002-12-03 | 2010-12-22 | ユセベ ファルマ ソシエテ アノニム | 抗体産生細胞を同定するためのアッセイ |
| GB0303337D0 (en) | 2003-02-13 | 2003-03-19 | Celltech R&D Ltd | Biological products |
| KR20120090094A (ko) | 2003-06-13 | 2012-08-16 | 바이오겐 아이덱 엠에이 인코포레이티드 | 아글리코실 항-ｃｄ154(ｃｄ40 리간드) 항체 및 이의 용도 |
| SI1639011T1 (sl) | 2003-06-30 | 2009-04-30 | Domantis Ltd | Pegilirana protitelesa z enojno domeno (dAb) |
| GB0315457D0 (en) | 2003-07-01 | 2003-08-06 | Celltech R&D Ltd | Biological products |
| GB0315450D0 (en) | 2003-07-01 | 2003-08-06 | Celltech R&D Ltd | Biological products |
| NZ580688A (en) | 2003-07-26 | 2012-03-30 | Biogen Idec Inc | Method of designing altered antibodies having improved antigen-binding affinity |
| EP2399936A3 (en) | 2004-07-26 | 2012-02-22 | Biogen Idec MA Inc. | Anti-CD154 antibodies |
| AU2005282700A1 (en) | 2004-09-02 | 2006-03-16 | Genentech, Inc. | Heteromultimeric molecules |
| US7563443B2 (en) | 2004-09-17 | 2009-07-21 | Domantis Limited | Monovalent anti-CD40L antibody polypeptides and compositions thereof |
| GB0425534D0 (en) | 2004-11-19 | 2004-12-22 | Celltech R&D Ltd | Process for obtaining antibodies |
| EP2125894B1 (en) | 2007-03-22 | 2018-12-19 | Biogen MA Inc. | Binding proteins, including antibodies, antibody derivatives and antibody fragments, that specifically bind cd154 and uses thereof |
| WO2008126785A1 (ja) * | 2007-04-06 | 2008-10-23 | Kyowa Hakko Kirin Co., Ltd. | 有用物質の製造法 |
| US7741088B2 (en) * | 2007-10-31 | 2010-06-22 | E.I. Dupont De Nemours And Company | Immobilized microbial nitrilase for production of glycolic acid |
| JP5217780B2 (ja) * | 2008-02-08 | 2013-06-19 | 味の素株式会社 | Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法 |
| US7662587B1 (en) | 2009-03-05 | 2010-02-16 | E. I. Du Pont De Nemours And Company | Gene knockout mutations that increase peptide production |
| IN2012DN02401A (enExample) | 2009-09-24 | 2015-08-21 | Ucb Pharma Sa | |
| US8470552B2 (en) | 2009-10-12 | 2013-06-25 | Keck Graduate Institute | Strategy to reduce lactic acid production and control PH in animal cell culture |
| CN105274170A (zh) | 2009-11-05 | 2016-01-27 | 弗·哈夫曼-拉罗切有限公司 | 分泌异源多肽的方法和组合物 |
| GB201000587D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000591D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial hoist strain |
| GB201000588D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201000590D0 (en) | 2010-01-14 | 2010-03-03 | Ucb Pharma Sa | Bacterial host strain |
| GB201001791D0 (en) | 2010-02-03 | 2010-03-24 | Ucb Pharma Sa | Process for obtaining antibodies |
| GB201012599D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Process for purifying proteins |
| PL2731973T3 (pl) | 2011-07-13 | 2018-04-30 | Ucb Biopharma Sprl | Bakteryjny szczep gospodarz wykazujący ekspresję dsbc |
| EP2546267A1 (en) | 2011-07-13 | 2013-01-16 | UCB Pharma S.A. | Bacterial host strain expressing recombinant DsbC |
| GB201208367D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Biological product |
-
2010
- 2010-09-23 IN IN2401DEN2012 patent/IN2012DN02401A/en unknown
- 2010-09-23 PL PL15182886T patent/PL2993231T3/pl unknown
- 2010-09-23 WO PCT/GB2010/001790 patent/WO2011036454A1/en not_active Ceased
- 2010-09-23 ES ES15182886.0T patent/ES2692811T3/es active Active
- 2010-09-23 CN CN201080047636.1A patent/CN102575241B/zh active Active
- 2010-09-23 PT PT15182886T patent/PT2993231T/pt unknown
- 2010-09-23 BR BR112012006670A patent/BR112012006670B8/pt active IP Right Grant
- 2010-09-23 CA CA2774692A patent/CA2774692C/en active Active
- 2010-09-23 SI SI201031758T patent/SI2993231T1/sl unknown
- 2010-09-23 US US13/497,987 patent/US9109216B2/en active Active
- 2010-09-23 JP JP2012530330A patent/JP6132551B2/ja active Active
- 2010-09-23 EP EP10757621A patent/EP2480661A1/en not_active Ceased
- 2010-09-23 LT LTEP15182886.0T patent/LT2993231T/lt unknown
- 2010-09-23 EP EP15182886.0A patent/EP2993231B1/en active Active
- 2010-09-23 AU AU2010299640A patent/AU2010299640B2/en active Active
- 2010-09-23 KR KR1020127010401A patent/KR101758942B1/ko active Active
- 2010-09-23 SM SM20180549T patent/SMT201800549T1/it unknown
- 2010-09-23 HU HUE15182886A patent/HUE040306T2/hu unknown
- 2010-09-23 SG SG10201606462WA patent/SG10201606462WA/en unknown
- 2010-09-23 DK DK15182886.0T patent/DK2993231T3/en active
-
2015
- 2015-08-17 US US14/827,408 patent/US9315770B2/en active Active
- 2015-12-22 JP JP2015250165A patent/JP2016116517A/ja active Pending
-
2016
- 2016-03-09 AU AU2016201536A patent/AU2016201536A1/en not_active Abandoned
- 2016-03-29 US US15/083,446 patent/US9550973B2/en active Active
-
2018
- 2018-09-18 HR HRP20181482TT patent/HRP20181482T1/hr unknown
- 2018-11-01 CY CY181101144T patent/CY1120947T1/el unknown
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