KR101758942B1 - 박테리아 숙주 균주 - Google Patents

박테리아 숙주 균주 Download PDF

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Publication number
KR101758942B1
KR101758942B1 KR1020127010401A KR20127010401A KR101758942B1 KR 101758942 B1 KR101758942 B1 KR 101758942B1 KR 1020127010401 A KR1020127010401 A KR 1020127010401A KR 20127010401 A KR20127010401 A KR 20127010401A KR 101758942 B1 KR101758942 B1 KR 101758942B1
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gene
cell
protein
mutant
ala
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KR20120083435A (ko
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마크 엘리스
데이비드 폴 험프리즈
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유씨비 파마, 에스.에이.
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Priority claimed from GB0916821A external-priority patent/GB0916821D0/en
Priority claimed from GBGB0916822.0A external-priority patent/GB0916822D0/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020127010401A 2009-09-24 2010-09-23 박테리아 숙주 균주 Active KR101758942B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0916821A GB0916821D0 (en) 2009-09-24 2009-09-24 Bacterial host strain
GB0916821.2 2009-09-24
GB0916822.0 2009-09-24
GBGB0916822.0A GB0916822D0 (en) 2009-09-24 2009-09-24 Bacterial host strain
PCT/GB2010/001790 WO2011036454A1 (en) 2009-09-24 2010-09-23 Bacterial host strain

Publications (2)

Publication Number Publication Date
KR20120083435A KR20120083435A (ko) 2012-07-25
KR101758942B1 true KR101758942B1 (ko) 2017-07-17

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Application Number Title Priority Date Filing Date
KR1020127010401A Active KR101758942B1 (ko) 2009-09-24 2010-09-23 박테리아 숙주 균주

Country Status (21)

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US (3) US9109216B2 (enExample)
EP (2) EP2480661A1 (enExample)
JP (2) JP6132551B2 (enExample)
KR (1) KR101758942B1 (enExample)
CN (1) CN102575241B (enExample)
AU (2) AU2010299640B2 (enExample)
BR (1) BR112012006670B8 (enExample)
CA (1) CA2774692C (enExample)
CY (1) CY1120947T1 (enExample)
DK (1) DK2993231T3 (enExample)
ES (1) ES2692811T3 (enExample)
HR (1) HRP20181482T1 (enExample)
HU (1) HUE040306T2 (enExample)
IN (1) IN2012DN02401A (enExample)
LT (1) LT2993231T (enExample)
PL (1) PL2993231T3 (enExample)
PT (1) PT2993231T (enExample)
SG (1) SG10201606462WA (enExample)
SI (1) SI2993231T1 (enExample)
SM (1) SMT201800549T1 (enExample)
WO (1) WO2011036454A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2012DN02401A (enExample) * 2009-09-24 2015-08-21 Ucb Pharma Sa
GB201000590D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000591D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
PL2731973T3 (pl) * 2011-07-13 2018-04-30 Ucb Biopharma Sprl Bakteryjny szczep gospodarz wykazujący ekspresję dsbc
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
EP3824906A1 (en) 2016-12-21 2021-05-26 Amgen Inc. Anti-tnf alpha antibody formulations
EP3774926A1 (en) 2018-04-05 2021-02-17 Bio-Rad ABD Serotec GmbH Display systems for proteins of interest
WO2020188350A1 (en) 2019-03-18 2020-09-24 Bio-Rad Abd Serotec Gmbh Protection of spytag-containing periplasmic fusion proteins from protease tsp and ompt degradation
CN110616181A (zh) * 2019-09-27 2019-12-27 北京理工大学 一株基于大肠杆菌改造的工程菌株
CN117965503B (zh) * 2024-02-02 2024-08-09 南京迪诺薇华生物科技有限公司 一种蛋白酶突变体及其在洗护肤品、化妆品中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002048376A2 (en) * 2000-12-14 2002-06-20 Genentech, Inc. Bacterial host strains

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264365A (en) 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US5508192A (en) 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
GB9215540D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US6027888A (en) 1996-04-05 2000-02-22 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells
US6083715A (en) 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
JP4249832B2 (ja) * 1998-12-28 2009-04-08 タカラバイオ株式会社 トリガーファクター発現プラスミド
AU767670B2 (en) * 1999-06-29 2003-11-20 Siga Technologies, Inc. DEGP periplasmic protease a new anti-infective target and an invitro assay for DEGP protease function
GB0006398D0 (en) 2000-03-16 2000-05-03 Novartis Ag Organic compounds
GB0013810D0 (en) 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
AU2001287040A1 (en) 2000-09-01 2002-03-13 Biogen, Inc. Methods of designing and producing novel compounds having improved binding affinity for cd154 or other trimeric proteins
AU8867501A (en) 2000-09-01 2002-03-13 Biogen Inc Co-crystal structure of monoclonal antibody 5c8 and cd154, and use thereof in drug design
US7041479B2 (en) 2000-09-06 2006-05-09 The Board Of Trustess Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
MXPA03005273A (es) 2000-12-14 2003-09-25 Genentech Inc Anticuerpos producidos procarioticamente y sus usos.
TWI334439B (en) 2001-08-01 2010-12-11 Centocor Inc Anti-tnf antibodies, compositions, methods and uses
EP1427744B1 (en) 2001-08-27 2007-12-26 Genentech, Inc. A system for antibody expression and assembly
GB0124317D0 (en) 2001-10-10 2001-11-28 Celltech R&D Ltd Biological products
IL161988A0 (en) 2001-11-16 2005-11-20 Corporation Idec Pharmaceutica Polycistronic expression of antibodies
GB0129105D0 (en) 2001-12-05 2002-01-23 Celltech R&D Ltd Expression control using variable intergenic sequences
EP1517921B1 (en) 2002-06-28 2006-06-07 Domantis Limited Dual specific ligands with increased serum half-life
AU2003287345A1 (en) 2002-10-31 2004-06-07 Genentech, Inc. Methods and compositions for increasing antibody production
JP4603894B2 (ja) 2002-12-03 2010-12-22 ユセベ ファルマ ソシエテ アノニム 抗体産生細胞を同定するためのアッセイ
GB0303337D0 (en) 2003-02-13 2003-03-19 Celltech R&D Ltd Biological products
KR20120090094A (ko) 2003-06-13 2012-08-16 바이오겐 아이덱 엠에이 인코포레이티드 아글리코실 항-ｃｄ154(ｃｄ40 리간드) 항체 및 이의 용도
SI1639011T1 (sl) 2003-06-30 2009-04-30 Domantis Ltd Pegilirana protitelesa z enojno domeno (dAb)
GB0315457D0 (en) 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
GB0315450D0 (en) 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
NZ580688A (en) 2003-07-26 2012-03-30 Biogen Idec Inc Method of designing altered antibodies having improved antigen-binding affinity
EP2399936A3 (en) 2004-07-26 2012-02-22 Biogen Idec MA Inc. Anti-CD154 antibodies
AU2005282700A1 (en) 2004-09-02 2006-03-16 Genentech, Inc. Heteromultimeric molecules
US7563443B2 (en) 2004-09-17 2009-07-21 Domantis Limited Monovalent anti-CD40L antibody polypeptides and compositions thereof
GB0425534D0 (en) 2004-11-19 2004-12-22 Celltech R&D Ltd Process for obtaining antibodies
EP2125894B1 (en) 2007-03-22 2018-12-19 Biogen MA Inc. Binding proteins, including antibodies, antibody derivatives and antibody fragments, that specifically bind cd154 and uses thereof
WO2008126785A1 (ja) * 2007-04-06 2008-10-23 Kyowa Hakko Kirin Co., Ltd. 有用物質の製造法
US7741088B2 (en) * 2007-10-31 2010-06-22 E.I. Dupont De Nemours And Company Immobilized microbial nitrilase for production of glycolic acid
JP5217780B2 (ja) * 2008-02-08 2013-06-19 味の素株式会社 Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
US7662587B1 (en) 2009-03-05 2010-02-16 E. I. Du Pont De Nemours And Company Gene knockout mutations that increase peptide production
IN2012DN02401A (enExample) 2009-09-24 2015-08-21 Ucb Pharma Sa
US8470552B2 (en) 2009-10-12 2013-06-25 Keck Graduate Institute Strategy to reduce lactic acid production and control PH in animal cell culture
CN105274170A (zh) 2009-11-05 2016-01-27 弗·哈夫曼-拉罗切有限公司 分泌异源多肽的方法和组合物
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000591D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000588D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000590D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201001791D0 (en) 2010-02-03 2010-03-24 Ucb Pharma Sa Process for obtaining antibodies
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
PL2731973T3 (pl) 2011-07-13 2018-04-30 Ucb Biopharma Sprl Bakteryjny szczep gospodarz wykazujący ekspresję dsbc
EP2546267A1 (en) 2011-07-13 2013-01-16 UCB Pharma S.A. Bacterial host strain expressing recombinant DsbC
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002048376A2 (en) * 2000-12-14 2002-06-20 Genentech, Inc. Bacterial host strains

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US9315770B2 (en) 2016-04-19
HK1172650A1 (zh) 2013-04-26
WO2011036454A1 (en) 2011-03-31
JP2016116517A (ja) 2016-06-30
EP2993231B1 (en) 2018-08-01
HUE040306T2 (hu) 2019-03-28
JP6132551B2 (ja) 2017-05-24
EP2993231A3 (en) 2016-05-11
PL2993231T3 (pl) 2019-01-31
US20160200810A1 (en) 2016-07-14
LT2993231T (lt) 2018-11-12
BR112012006670B8 (pt) 2022-03-03
IN2012DN02401A (enExample) 2015-08-21
DK2993231T3 (en) 2018-10-22
CY1120947T1 (el) 2019-12-11
ES2692811T3 (es) 2018-12-05
CA2774692A1 (en) 2011-03-31
US20150344840A1 (en) 2015-12-03
BR112012006670A2 (pt) 2015-09-08
HRP20181482T1 (hr) 2018-11-02
CN102575241B (zh) 2016-02-24
SI2993231T1 (sl) 2018-10-30
AU2010299640A1 (en) 2012-04-12
SMT201800549T1 (it) 2018-11-09
US9109216B2 (en) 2015-08-18
BR112012006670B1 (pt) 2021-08-03
AU2016201536A1 (en) 2016-03-24
EP2480661A1 (en) 2012-08-01
US20120258492A1 (en) 2012-10-11
CA2774692C (en) 2017-12-19
AU2010299640B2 (en) 2016-02-25
KR20120083435A (ko) 2012-07-25
SG10201606462WA (en) 2016-09-29
US9550973B2 (en) 2017-01-24
CN102575241A (zh) 2012-07-11
EP2993231A2 (en) 2016-03-09
JP2013505714A (ja) 2013-02-21
PT2993231T (pt) 2018-11-12

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