PT2401383E - Métodos para seleccionar células eucarióticas que expressam uma proteína heteróloga - Google Patents
Métodos para seleccionar células eucarióticas que expressam uma proteína heterólogaInfo
- Publication number
- PT2401383E PT2401383E PT107096877T PT10709687T PT2401383E PT 2401383 E PT2401383 E PT 2401383E PT 107096877 T PT107096877 T PT 107096877T PT 10709687 T PT10709687 T PT 10709687T PT 2401383 E PT2401383 E PT 2401383E
- Authority
- PT
- Portugal
- Prior art keywords
- host cells
- interest
- product
- methods
- selecting
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09154000 | 2009-02-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT2401383E true PT2401383E (pt) | 2013-12-16 |
Family
ID=40451117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT107096877T PT2401383E (pt) | 2009-02-27 | 2010-02-26 | Métodos para seleccionar células eucarióticas que expressam uma proteína heteróloga |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US8962274B2 (pt) |
| EP (1) | EP2401383B1 (pt) |
| JP (2) | JP5745431B2 (pt) |
| KR (1) | KR101706399B1 (pt) |
| CN (1) | CN102333875B (pt) |
| AU (1) | AU2010219088B2 (pt) |
| BR (1) | BRPI1008762B8 (pt) |
| CA (1) | CA2753813C (pt) |
| DK (1) | DK2401383T3 (pt) |
| ES (1) | ES2440321T3 (pt) |
| IL (1) | IL214620A (pt) |
| MX (1) | MX2011009023A (pt) |
| PL (1) | PL2401383T3 (pt) |
| PT (1) | PT2401383E (pt) |
| RU (1) | RU2551229C2 (pt) |
| SG (1) | SG173457A1 (pt) |
| WO (1) | WO2010097239A1 (pt) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2011009025A (es) | 2009-02-27 | 2011-09-28 | Novartis Ag | Sistema de vector de expresion que comprende dos marcadores de seleccion. |
| EP2401383B1 (en) * | 2009-02-27 | 2013-09-18 | Novartis AG | Methods for selecting eukaryotic cells expressing a heterologous protein |
| WO2013041487A1 (en) * | 2011-09-21 | 2013-03-28 | F. Hoffmann-La Roche Ag | Co2 profile cultivation |
| DK3412684T3 (da) | 2013-07-31 | 2022-07-04 | Novartis Ag | Nye selektionsvektorer og fremgangsmåder til selektering af eukaryotiske værtsceller |
| SG11201706617SA (en) * | 2015-04-03 | 2017-09-28 | Selexis Sa | Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells |
| UY37758A (es) | 2017-06-12 | 2019-01-31 | Novartis Ag | Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2585532B2 (ja) * | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| WO1991003554A1 (en) | 1989-09-01 | 1991-03-21 | Genentech, Inc. | Cells transfected with nucleic acid encoding gtp |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| AR008077A1 (es) | 1996-07-26 | 1999-12-09 | Talarico Salinas Laura Beatriz | Un polipeptido de fusion o una sal del mismo, su uso, un proceso para prepararlos, una composicion farmaceutica que los comprende, y un vector. |
| JP4031154B2 (ja) * | 1999-07-30 | 2008-01-09 | 独立行政法人科学技術振興機構 | 遺伝子増幅細胞の迅速選択法 |
| EP1349873B1 (en) | 2000-09-14 | 2009-04-01 | Beth Israel Deaconess Medical Center, Inc. | Modulation of il-2- and il-15-mediated t cell responses |
| EP1453948A2 (en) | 2001-11-28 | 2004-09-08 | Sandoz Gmbh | Cell culture process |
| US7384744B2 (en) * | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| TW200513351A (en) * | 2003-07-17 | 2005-04-16 | Availvs Corp | High pressure water jet surface cutting device and cutting method |
| EP1533617A1 (en) | 2003-11-19 | 2005-05-25 | RMF Dictagene S.A. | Angiogenesis inhibiting molecules, their selection, production and their use in the treatment and diagnosis of cancer |
| EP1953222A1 (en) | 2007-01-24 | 2008-08-06 | Boehringer Ingelheim Pharma GmbH & Co. KG | Improvement of cell growth |
| SG182953A1 (en) * | 2007-03-30 | 2012-08-30 | Abbott Lab | Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells |
| EP2401383B1 (en) * | 2009-02-27 | 2013-09-18 | Novartis AG | Methods for selecting eukaryotic cells expressing a heterologous protein |
-
2010
- 2010-02-26 EP EP10709687.7A patent/EP2401383B1/en active Active
- 2010-02-26 CA CA2753813A patent/CA2753813C/en active Active
- 2010-02-26 BR BRPI1008762A patent/BRPI1008762B8/pt active IP Right Grant
- 2010-02-26 PT PT107096877T patent/PT2401383E/pt unknown
- 2010-02-26 RU RU2011139183/10A patent/RU2551229C2/ru active
- 2010-02-26 DK DK10709687.7T patent/DK2401383T3/da active
- 2010-02-26 JP JP2011551443A patent/JP5745431B2/ja active Active
- 2010-02-26 AU AU2010219088A patent/AU2010219088B2/en active Active
- 2010-02-26 MX MX2011009023A patent/MX2011009023A/es active IP Right Grant
- 2010-02-26 PL PL10709687T patent/PL2401383T3/pl unknown
- 2010-02-26 ES ES10709687.7T patent/ES2440321T3/es active Active
- 2010-02-26 SG SG2011054087A patent/SG173457A1/en unknown
- 2010-02-26 KR KR1020117022540A patent/KR101706399B1/ko active IP Right Grant
- 2010-02-26 WO PCT/EP2010/001223 patent/WO2010097239A1/en active Application Filing
- 2010-02-26 US US13/203,546 patent/US8962274B2/en active Active
- 2010-02-26 CN CN201080009517.7A patent/CN102333875B/zh active Active
-
2011
- 2011-08-11 IL IL214620A patent/IL214620A/en active IP Right Grant
-
2014
- 2014-02-06 US US14/174,228 patent/US9315844B2/en active Active
- 2014-12-24 JP JP2014261016A patent/JP2015107120A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| KR20110123783A (ko) | 2011-11-15 |
| AU2010219088B2 (en) | 2012-11-01 |
| WO2010097239A1 (en) | 2010-09-02 |
| US8962274B2 (en) | 2015-02-24 |
| CA2753813C (en) | 2018-12-11 |
| BRPI1008762B1 (pt) | 2020-10-27 |
| PL2401383T3 (pl) | 2014-02-28 |
| CN102333875B (zh) | 2014-04-16 |
| BRPI1008762A2 (pt) | 2015-08-25 |
| US20110306095A1 (en) | 2011-12-15 |
| US20140154739A1 (en) | 2014-06-05 |
| EP2401383A1 (en) | 2012-01-04 |
| RU2551229C2 (ru) | 2015-05-20 |
| JP2012518991A (ja) | 2012-08-23 |
| AU2010219088A1 (en) | 2011-08-18 |
| BRPI1008762B8 (pt) | 2021-05-25 |
| IL214620A (en) | 2016-02-29 |
| DK2401383T3 (da) | 2013-12-16 |
| KR101706399B1 (ko) | 2017-02-13 |
| MX2011009023A (es) | 2011-09-28 |
| SG173457A1 (en) | 2011-09-29 |
| CA2753813A1 (en) | 2010-09-02 |
| IL214620A0 (en) | 2011-09-27 |
| JP2015107120A (ja) | 2015-06-11 |
| RU2011139183A (ru) | 2013-04-10 |
| EP2401383B1 (en) | 2013-09-18 |
| CN102333875A (zh) | 2012-01-25 |
| ES2440321T3 (es) | 2014-01-28 |
| US9315844B2 (en) | 2016-04-19 |
| JP5745431B2 (ja) | 2015-07-08 |
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