SG11201706617SA - Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells - Google Patents

Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells

Info

Publication number
SG11201706617SA
SG11201706617SA SG11201706617SA SG11201706617SA SG11201706617SA SG 11201706617S A SG11201706617S A SG 11201706617SA SG 11201706617S A SG11201706617S A SG 11201706617SA SG 11201706617S A SG11201706617S A SG 11201706617SA SG 11201706617S A SG11201706617S A SG 11201706617SA
Authority
SG
Singapore
Prior art keywords
vitamins
proteins
mammalian cells
recombinant protein
protein production
Prior art date
Application number
SG11201706617SA
Inventor
Nicolas Mermod
Lucille Pourcel
Pierre-Alain Girod
Fourn Valérie Le
Original Assignee
Selexis Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Selexis Sa filed Critical Selexis Sa
Publication of SG11201706617SA publication Critical patent/SG11201706617SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
SG11201706617SA 2015-04-03 2016-04-01 Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells SG11201706617SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562142516P 2015-04-03 2015-04-03
PCT/EP2016/057228 WO2016156574A1 (en) 2015-04-03 2016-04-01 Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells

Publications (1)

Publication Number Publication Date
SG11201706617SA true SG11201706617SA (en) 2017-09-28

Family

ID=55862725

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201706617SA SG11201706617SA (en) 2015-04-03 2016-04-01 Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells

Country Status (19)

Country Link
US (1) US20180066268A1 (en)
EP (1) EP3277818B1 (en)
JP (1) JP6767991B2 (en)
KR (1) KR102622657B1 (en)
CN (1) CN107624133A (en)
AU (1) AU2016240211B2 (en)
CA (1) CA2976723A1 (en)
DK (1) DK3277818T3 (en)
ES (1) ES2773529T3 (en)
HK (1) HK1244029A1 (en)
HU (1) HUE047656T2 (en)
IL (1) IL254799B (en)
LT (1) LT3277818T (en)
PL (1) PL3277818T3 (en)
PT (1) PT3277818T (en)
RU (1) RU2736705C2 (en)
SG (1) SG11201706617SA (en)
SI (1) SI3277818T1 (en)
WO (1) WO2016156574A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3068685A1 (en) * 2017-06-27 2019-01-03 Ajinomoto Co., Inc. Riboflavin derivative-containing medium
EP3870708A1 (en) 2018-10-24 2021-09-01 Selexis S.A. Expression systems, recombinant cells and uses thereof
JP7123168B2 (en) * 2018-12-11 2022-08-22 富士フイルム株式会社 Animal cell, method for producing animal cell, and method for producing target protein

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2310686C2 (en) * 1999-06-25 2007-11-20 Басф Акциенгезелльшафт Corynebacterium glutamicum genes encoding proteins participating in carbon metabolism and energy producing
AU2002240312A1 (en) * 2001-02-08 2002-08-19 Pankaj Paranjp Enhanced oral and transcompartmental delivery of therapeutic or diagnostic agents using polymer conjugates
PT2227549E (en) * 2007-12-21 2015-10-08 Novartis Ag Selection system for eukaryotic cell culture based on a membrane-bound folate receptor gene
DK2401383T3 (en) * 2009-02-27 2013-12-16 Novartis Ag Methods for selecting eukaryotic cells expressing a heterologous protein
JP5801207B2 (en) * 2009-02-27 2015-10-28 ノバルティス アーゲー Expression vector system containing two types of selectable markers

Also Published As

Publication number Publication date
EP3277818B1 (en) 2020-01-08
KR102622657B1 (en) 2024-01-10
HUE047656T2 (en) 2020-05-28
JP6767991B2 (en) 2020-10-14
RU2017128122A3 (en) 2020-03-19
PT3277818T (en) 2020-03-03
WO2016156574A1 (en) 2016-10-06
ES2773529T3 (en) 2020-07-13
EP3277818A1 (en) 2018-02-07
CA2976723A1 (en) 2016-10-06
DK3277818T3 (en) 2020-02-24
HK1244029A1 (en) 2018-07-27
LT3277818T (en) 2020-02-25
RU2736705C2 (en) 2020-11-19
US20180066268A1 (en) 2018-03-08
IL254799A0 (en) 2017-12-31
AU2016240211A1 (en) 2017-08-10
JP2018509904A (en) 2018-04-12
RU2017128122A (en) 2019-05-06
SI3277818T1 (en) 2020-04-30
CN107624133A (en) 2018-01-23
PL3277818T3 (en) 2020-06-15
KR20170133327A (en) 2017-12-05
AU2016240211B2 (en) 2022-03-31
IL254799B (en) 2021-04-29

Similar Documents

Publication Publication Date Title
HK1248755A1 (en) Protein delivery in primary hematopoietic cells
HK1222662A1 (en) In vitro production of red blood cells with sortaggable proteins
HK1250021A1 (en) Recombinant promoters and vectors for protein expression in liver and use thereof
HK1215299A1 (en) Means and methods for the determination of the biological activity of neurotoxin polypeptides in cells
PL3310805T3 (en) Pd-1-cd28 fusion proteins and their use in medicine
IL248361A0 (en) Recombinant host cell for expressing proteins of interest
PL3036320T3 (en) Control of protein glycosylation by culture medium supplementation and cell culture process parameters
HK1244029A1 (en) Use of vitamins and vitamin metabolic genes and proteins for recombinant protein production in mammalian cells
EP3180463A4 (en) Detecting residual host cell proteins in recombinant protein preparations
HUE063273T2 (en) Production of heteromultimeric proteins using mammalian cells
MY194277A (en) Omega-hydroxylase-related fusion polypeptide variants with improved properties
EP3215604A4 (en) Methods of recombinant protein expression in a cell comprising reduced udp-galactose transporter activity
SG11201600682PA (en) Expression vector for production of recombinant proteins in prokaryotic host cells
HK1219979A1 (en) Artificial dna sequence with optimized leader function in 5 (5-utr) for the over-expression of recombinant proteins in plants and method for the production of recombinant proteins in plants 5(5-utr) dna
EP3322800A4 (en) Methods of making red blood cells and platelets in vitro and uses thereof
IL254732A0 (en) In vitro preservation of therapeutic cells
IL284803A (en) Methods of producing multimeric proteins in eukaryotic host cells
EP3256578B8 (en) Polypeptides for engineering integrase chimeric proteins and their use in gene therapy
SI3218514T1 (en) Predicting genetically stable recombinant protein production in early cell line development