PT2291540E - Dispositivo microfluídico funcionalizado para imunofluorescência - Google Patents

Dispositivo microfluídico funcionalizado para imunofluorescência Download PDF

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Publication number
PT2291540E
PT2291540E PT107059859T PT10705985T PT2291540E PT 2291540 E PT2291540 E PT 2291540E PT 107059859 T PT107059859 T PT 107059859T PT 10705985 T PT10705985 T PT 10705985T PT 2291540 E PT2291540 E PT 2291540E
Authority
PT
Portugal
Prior art keywords
immunofluorescence
microfluidic device
functionalized microfluidic
functionalized
microfluidic
Prior art date
Application number
PT107059859T
Other languages
English (en)
Inventor
Roberta Carbone
Emanuele Barborini
Dario Bandiera
Original Assignee
Tethis S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tethis S P A filed Critical Tethis S P A
Publication of PT2291540E publication Critical patent/PT2291540E/pt

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G01N33/5304—Reaction vessels, e.g. agglutination plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Inorganic Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
PT107059859T 2009-01-26 2010-01-26 Dispositivo microfluídico funcionalizado para imunofluorescência PT2291540E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2009/000465 WO2010083852A1 (en) 2009-01-26 2009-01-26 Functionalized microfluidic device for immunofluorescence

Publications (1)

Publication Number Publication Date
PT2291540E true PT2291540E (pt) 2014-03-03

Family

ID=40566192

Family Applications (1)

Application Number Title Priority Date Filing Date
PT107059859T PT2291540E (pt) 2009-01-26 2010-01-26 Dispositivo microfluídico funcionalizado para imunofluorescência

Country Status (15)

Country Link
US (1) US9364830B2 (pt)
EP (1) EP2291540B1 (pt)
JP (1) JP5719313B2 (pt)
CN (1) CN102308005B (pt)
BR (1) BRPI1007011A2 (pt)
CA (1) CA2750682C (pt)
DK (1) DK2291540T3 (pt)
ES (1) ES2446277T3 (pt)
HK (1) HK1154637A1 (pt)
HR (1) HRP20140042T1 (pt)
IL (1) IL214238A (pt)
MX (1) MX2011007643A (pt)
PL (1) PL2291540T3 (pt)
PT (1) PT2291540E (pt)
WO (2) WO2010083852A1 (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011034678A1 (en) * 2009-09-21 2011-03-24 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Use of superhydrophobic surfaces for liquid agglutination assays
WO2012061818A1 (en) * 2010-11-05 2012-05-10 Life Technologies Corporation Flowcells and flowcell reaction chambers
JP6084370B2 (ja) * 2011-05-10 2017-02-22 国立大学法人 東京大学 組織化学用自動反応装置
JP6004403B2 (ja) * 2012-02-23 2016-10-05 国立大学法人島根大学 マイクロ流路チップ
CN104777101A (zh) * 2015-04-24 2015-07-15 王丹凤 一种观察细胞的方法
IT201600099710A1 (it) * 2016-10-05 2018-04-05 Tethis S P A Portacampioni per analisi di spettrometria di massa in modalità maldi, produzione ed uso del portacampioni
IT201700085439A1 (it) * 2017-07-26 2019-01-26 Tethis S P A Metodo di immobilizzazione di campioni biologici a scopo analitico e diagnostico
KR101952443B1 (ko) * 2018-08-23 2019-02-26 주식회사 아스트로젠 신규한 마그네슘­세리네이트 화합물 및 이의 용도
CN109060688A (zh) * 2018-08-27 2018-12-21 南京采薇且歌信息科技有限公司 一种高精度纳米传感器及其应用
JP7347931B2 (ja) * 2018-12-26 2023-09-20 スリーエム イノベイティブ プロパティズ カンパニー マイクロ流体デバイス用フィルム、マイクロ流体デバイス及びその製造方法
JP7356241B2 (ja) * 2019-03-18 2023-10-04 フコク物産株式会社 マイクロ流体デバイス
TWI762811B (zh) * 2019-08-30 2022-05-01 國立臺灣科技大學 負載有多孔隙奈米顆粒結構之多孔隙基材及其負載製程
CN110553888B (zh) * 2019-09-16 2022-07-12 中国农业科学院特产研究所 一种卵母细胞免疫荧光染色后快速压片的方法
CN110951834A (zh) * 2019-12-10 2020-04-03 中国科学院深圳先进技术研究院 一种基于微流控芯片的细胞荧光原位杂交方法及其应用

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EP0752099A1 (en) * 1994-02-09 1997-01-08 Abbott Laboratories Diagnostic flow cell device
US6613560B1 (en) * 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
WO1998055651A1 (en) * 1997-06-05 1998-12-10 University Of Massachusetts Method for determining rate of movement of a molecule in a living cell
JP2002531470A (ja) * 1998-12-01 2002-09-24 シントリクス バイオチップ， インコーポレイテッド 支持体表面上で化学反応のアレイを行うための方法および組成物
IT1310029B1 (it) 1999-02-26 2002-02-05 Ist Naz Fisica Della Materia Vaporizzatore a microplasma pulsato.
JP3993372B2 (ja) * 2000-09-13 2007-10-17 独立行政法人理化学研究所 リアクタの製造方法
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FR2818287B1 (fr) * 2000-12-14 2003-01-17 Commissariat Energie Atomique Support solide pour l'immobilisation d'oligonucleotides
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Also Published As

Publication number Publication date
CN102308005A (zh) 2012-01-04
AU2010207677A1 (en) 2011-09-08
US20110281267A1 (en) 2011-11-17
CN102308005B (zh) 2015-07-15
PL2291540T3 (pl) 2014-05-30
IL214238A (en) 2017-11-30
CA2750682C (en) 2016-10-25
MX2011007643A (es) 2011-10-28
WO2010084196A1 (en) 2010-07-29
EP2291540B1 (en) 2013-11-20
WO2010083852A1 (en) 2010-07-29
CA2750682A1 (en) 2010-07-29
US9364830B2 (en) 2016-06-14
JP2012515908A (ja) 2012-07-12
EP2291540A1 (en) 2011-03-09
HK1154637A1 (en) 2012-04-27
DK2291540T3 (en) 2014-02-17
IL214238A0 (en) 2011-09-27
BRPI1007011A2 (pt) 2016-03-29
JP5719313B2 (ja) 2015-05-13
AU2010207677A2 (en) 2011-09-08
ES2446277T3 (es) 2014-03-06
HRP20140042T1 (hr) 2014-02-28

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