PL439656A1 - Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K - Google Patents
Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy KInfo
- Publication number
- PL439656A1 PL439656A1 PL439656A PL43965621A PL439656A1 PL 439656 A1 PL439656 A1 PL 439656A1 PL 439656 A PL439656 A PL 439656A PL 43965621 A PL43965621 A PL 43965621A PL 439656 A1 PL439656 A1 PL 439656A1
- Authority
- PL
- Poland
- Prior art keywords
- proteinase
- mutant
- zymogen
- preparing
- expression plasmid
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21064—Peptidase K (3.4.21.64)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/102—Plasmid DNA for yeast
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
W niniejszym zgłoszeniu ujawniono mutanta zymogenu proteinazy K z Tritirachium album zawierającego mutację zwiększającą wydajność ekspresji w komórkach drożdżowych oraz ułatwiającą dezaktywację dojrzałej proteinazy K w warunkach stresowych oraz sposób jego otrzymywania.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL439656A PL244825B1 (pl) | 2021-11-26 | 2021-11-26 | Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K |
PCT/PL2022/050084 WO2023096513A1 (en) | 2021-11-26 | 2022-11-25 | Tritirachium album proteinase k mutant and its zymogen, expression plasmid, recombinant pichia pastoris strain and method of producing the mature form of proteinase k mutant |
EP22823168.4A EP4437115A1 (en) | 2021-11-26 | 2022-11-25 | Tritirachium album proteinase k mutant and its zymogen, expression plasmid, recombinant pichia pastoris strain and method of producing the mature form of proteinase k mutant |
CN202280077956.4A CN118302531A (zh) | 2021-11-26 | 2022-11-25 | 白色念球菌蛋白酶k突变体及其酶原、表达质粒、重组巴斯德毕赤酵母菌株以及产生蛋白酶k突变体成熟形式的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL439656A PL244825B1 (pl) | 2021-11-26 | 2021-11-26 | Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K |
Publications (2)
Publication Number | Publication Date |
---|---|
PL439656A1 true PL439656A1 (pl) | 2023-05-29 |
PL244825B1 PL244825B1 (pl) | 2024-03-11 |
Family
ID=84520096
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL439656A PL244825B1 (pl) | 2021-11-26 | 2021-11-26 | Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP4437115A1 (pl) |
CN (1) | CN118302531A (pl) |
PL (1) | PL244825B1 (pl) |
WO (1) | WO2023096513A1 (pl) |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2803907C3 (de) | 1978-01-30 | 1982-09-23 | Gewerkschaft Eisenhütte Westfalia, 4670 Lünen | Lösbare Gelenkverbindung für den untertägigen Bergbau und ihre Verwendung an einem Strebausbaugestell |
DE10105911A1 (de) | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe |
PL213045B1 (pl) * | 2008-02-08 | 2013-01-31 | Kur Jozef Sachadyn Pawel Dna Gdansk Ii Spolka Cywilna | Mutant proteinazy K, sposób jego otrzymywania oraz sekwencje i szczepy wykorzystywane w tym sposobie |
CN102839165B (zh) * | 2012-09-26 | 2014-12-10 | 金普诺安生物科技(苏州)有限公司 | 基因突变型重组蛋白酶k及其工业化生产方法 |
GB201803654D0 (en) | 2018-03-07 | 2018-04-25 | Arcticzymes As | Thermolabile proteinases |
CN109280656B (zh) * | 2018-10-23 | 2021-06-15 | 大连博格林生物科技有限公司 | 重组布氏白僵菌蛋白酶k突变体pk-m1及制备方法 |
US20200199565A1 (en) | 2018-12-20 | 2020-06-25 | New England Biolabs, Inc. | Proteinases with Improved Properties |
CN113215138B (zh) * | 2021-06-02 | 2022-11-22 | 武汉瀚海新酶生物科技有限公司 | 一种热稳定性提高的蛋白酶k突变体 |
-
2021
- 2021-11-26 PL PL439656A patent/PL244825B1/pl unknown
-
2022
- 2022-11-25 CN CN202280077956.4A patent/CN118302531A/zh active Pending
- 2022-11-25 WO PCT/PL2022/050084 patent/WO2023096513A1/en active Application Filing
- 2022-11-25 EP EP22823168.4A patent/EP4437115A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
PL244825B1 (pl) | 2024-03-11 |
EP4437115A1 (en) | 2024-10-02 |
WO2023096513A1 (en) | 2023-06-01 |
CN118302531A (zh) | 2024-07-05 |
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