WO2016171224A9 - タンパク質の分泌生産法 - Google Patents
タンパク質の分泌生産法 Download PDFInfo
- Publication number
- WO2016171224A9 WO2016171224A9 PCT/JP2016/062675 JP2016062675W WO2016171224A9 WO 2016171224 A9 WO2016171224 A9 WO 2016171224A9 JP 2016062675 W JP2016062675 W JP 2016062675W WO 2016171224 A9 WO2016171224 A9 WO 2016171224A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- secretory production
- heterologous protein
- protein
- coryneform bacterium
- secretory
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/13—Protein-histidine kinases (2.7.13)
- C12Y207/13003—Histidine kinase (2.7.13.3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020177034151A KR102619848B1 (ko) | 2015-04-24 | 2016-04-21 | 단백질의 분비 생산법 |
EP16783245.0A EP3287523B1 (en) | 2015-04-24 | 2016-04-21 | Method for secretory production of protein |
CN201680023752.7A CN107532163A (zh) | 2015-04-24 | 2016-04-21 | 蛋白质的分泌生产方法 |
AU2016253217A AU2016253217B2 (en) | 2015-04-24 | 2016-04-21 | Method for secretory production of protein |
DK16783245.0T DK3287523T3 (da) | 2015-04-24 | 2016-04-21 | Fremgangsmåde til sekretorisk fremstilling af protein |
JP2017514190A JP6741000B2 (ja) | 2015-04-24 | 2016-04-21 | タンパク質の分泌生産法 |
US15/790,495 US10538798B2 (en) | 2015-04-24 | 2017-10-23 | Method for secretory production of protein |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015089046 | 2015-04-24 | ||
JP2015-089046 | 2015-04-24 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/790,495 Continuation US10538798B2 (en) | 2015-04-24 | 2017-10-23 | Method for secretory production of protein |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2016171224A1 WO2016171224A1 (ja) | 2016-10-27 |
WO2016171224A9 true WO2016171224A9 (ja) | 2017-09-28 |
Family
ID=57143158
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2016/062675 WO2016171224A1 (ja) | 2015-04-24 | 2016-04-21 | タンパク質の分泌生産法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US10538798B2 (ja) |
EP (1) | EP3287523B1 (ja) |
JP (1) | JP6741000B2 (ja) |
KR (1) | KR102619848B1 (ja) |
CN (1) | CN107532163A (ja) |
AU (1) | AU2016253217B2 (ja) |
DK (1) | DK3287523T3 (ja) |
WO (1) | WO2016171224A1 (ja) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2017347016B2 (en) | 2016-10-21 | 2021-09-23 | Ajinomoto Co., Inc. | Secretory production method for protein |
EP3530747A4 (en) | 2016-10-21 | 2020-07-22 | Ajinomoto Co., Inc. | SECRETORY PROTEIN PRODUCTION PROCESS |
WO2019078095A1 (en) | 2017-10-16 | 2019-04-25 | Ajinomoto Co., Inc. | PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY |
CN108330095B (zh) * | 2018-03-01 | 2020-12-29 | 江南大学 | 一种积累n-乙酰神经氨酸的重组谷氨酸棒杆菌及其应用 |
AU2019256065A1 (en) * | 2018-04-20 | 2020-11-26 | Ajinomoto Co., Inc. | Method for secretory production of protein |
CN110511953B (zh) * | 2018-08-31 | 2022-09-30 | 江南大学 | 一种适用于谷氨酸棒杆菌的重组表达载体、外源蛋白表达系统、应用和木聚糖酶的制备方法 |
WO2020071538A1 (en) | 2018-10-05 | 2020-04-09 | Ajinomoto Co., Inc. | Method for producing target substance by bacterial fermentation |
CN112930359A (zh) * | 2018-10-25 | 2021-06-08 | 味之素株式会社 | 蛋白质的分泌生产方法 |
JPWO2020090979A1 (ja) | 2018-10-31 | 2021-09-24 | 味の素株式会社 | 抗体に対する親和性物質、切断性部分および反応性基を有する化合物またはその塩 |
CN111434770B (zh) * | 2019-01-11 | 2022-12-06 | 华东师范大学 | 蛋白质谷氨酰胺酶酶原在枯草芽孢杆菌中的表达和自加工 |
EP4071178A1 (en) | 2019-12-06 | 2022-10-12 | Ajinomoto Co., Inc. | Method for producing peptide having physiological activity, and peptide comprising short linker |
CN117642511A (zh) | 2021-07-07 | 2024-03-01 | 味之素株式会社 | 含非天然氨基酸的蛋白质的分泌生产方法 |
CA3228922A1 (en) * | 2021-10-11 | 2023-04-20 | Ajinomoto Co., Inc. | Bacterium modified to express heterologous tat protein |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS592689A (ja) * | 1982-06-04 | 1984-01-09 | Handai Biseibutsubiyou Kenkyukai | 強力な遺伝子発現能を有する新規レプリコンの作成法 |
US4965197A (en) | 1987-06-12 | 1990-10-23 | Massachusetts Institute Of Technology | Coryneform expression and secretion system |
WO1993003158A1 (fr) | 1991-07-30 | 1993-02-18 | Orsan | Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries |
JPH11169182A (ja) | 1997-12-10 | 1999-06-29 | Mitsubishi Chemical Corp | コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子 |
CA2391961C (en) | 1999-09-30 | 2008-12-02 | Yoshimi Kikuchi | Process for producing transglutaminase |
RU2264463C2 (ru) | 2001-03-30 | 2005-11-20 | Адзиномото Ко., Инк. | Способ секреторной продукции белка |
GB0224082D0 (en) * | 2002-10-16 | 2002-11-27 | Celltech R&D Ltd | Biological products |
CN100554431C (zh) * | 2004-04-20 | 2009-10-28 | 味之素株式会社 | 生产蛋白质的方法 |
DK1748077T3 (da) | 2004-04-20 | 2013-04-08 | Ajinomoto Kk | Fremgangsmåde til fremstilling af protein |
EP2080769A3 (en) * | 2004-07-02 | 2010-12-01 | Metanomics GmbH | Process for the production of fine chemicals |
CN101675069A (zh) * | 2004-07-02 | 2010-03-17 | 梅坦诺米克斯有限公司 | 产生精细化学品的方法 |
CA2677179C (en) * | 2007-01-31 | 2016-02-16 | Dow Global Technologies Inc. | Bacterial leader sequences for increased expression |
CN103917656B (zh) * | 2011-10-25 | 2016-09-28 | 味之素株式会社 | 蛋白质的分泌产生方法 |
IN2014CN04002A (ja) | 2011-11-02 | 2015-10-23 | Ajinomoto Kk | |
AU2012333451B2 (en) | 2011-11-02 | 2015-05-21 | Ajinomoto Co., Inc. | Method for secretory production of protein |
JPWO2013118544A1 (ja) * | 2012-02-08 | 2015-05-11 | 味の素株式会社 | タンパク質の分泌生産法 |
US9478749B2 (en) | 2013-03-28 | 2016-10-25 | Semiconductor Energy Laboratory Co., Ltd. | Anthracene compound, light-emitting element, light-emitting device, electronic appliance, and lighting device |
-
2016
- 2016-04-21 KR KR1020177034151A patent/KR102619848B1/ko active IP Right Grant
- 2016-04-21 EP EP16783245.0A patent/EP3287523B1/en active Active
- 2016-04-21 AU AU2016253217A patent/AU2016253217B2/en active Active
- 2016-04-21 WO PCT/JP2016/062675 patent/WO2016171224A1/ja unknown
- 2016-04-21 CN CN201680023752.7A patent/CN107532163A/zh active Pending
- 2016-04-21 JP JP2017514190A patent/JP6741000B2/ja active Active
- 2016-04-21 DK DK16783245.0T patent/DK3287523T3/da active
-
2017
- 2017-10-23 US US15/790,495 patent/US10538798B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP3287523A1 (en) | 2018-02-28 |
KR20170140351A (ko) | 2017-12-20 |
CN107532163A (zh) | 2018-01-02 |
AU2016253217A1 (en) | 2017-11-23 |
US10538798B2 (en) | 2020-01-21 |
EP3287523B1 (en) | 2022-06-29 |
AU2016253217B2 (en) | 2021-09-02 |
US20180037918A1 (en) | 2018-02-08 |
WO2016171224A1 (ja) | 2016-10-27 |
DK3287523T3 (da) | 2022-07-25 |
EP3287523A4 (en) | 2018-09-05 |
KR102619848B1 (ko) | 2024-01-04 |
JP6741000B2 (ja) | 2020-08-19 |
JPWO2016171224A1 (ja) | 2018-02-22 |
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