WO2016171224A9 - タンパク質の分泌生産法 - Google Patents

タンパク質の分泌生産法 Download PDF

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Publication number
WO2016171224A9
WO2016171224A9 PCT/JP2016/062675 JP2016062675W WO2016171224A9 WO 2016171224 A9 WO2016171224 A9 WO 2016171224A9 JP 2016062675 W JP2016062675 W JP 2016062675W WO 2016171224 A9 WO2016171224 A9 WO 2016171224A9
Authority
WO
WIPO (PCT)
Prior art keywords
secretory production
heterologous protein
protein
coryneform bacterium
secretory
Prior art date
Application number
PCT/JP2016/062675
Other languages
English (en)
French (fr)
Other versions
WO2016171224A1 (ja
Inventor
吉彦 松田
悠美 伊藤
由香里 鹿嶋
奈緒子 山田
典子 鶴井
寛 板屋
Original Assignee
味の素株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 味の素株式会社 filed Critical 味の素株式会社
Priority to KR1020177034151A priority Critical patent/KR102619848B1/ko
Priority to EP16783245.0A priority patent/EP3287523B1/en
Priority to CN201680023752.7A priority patent/CN107532163A/zh
Priority to AU2016253217A priority patent/AU2016253217B2/en
Priority to DK16783245.0T priority patent/DK3287523T3/da
Priority to JP2017514190A priority patent/JP6741000B2/ja
Publication of WO2016171224A1 publication Critical patent/WO2016171224A1/ja
Publication of WO2016171224A9 publication Critical patent/WO2016171224A9/ja
Priority to US15/790,495 priority patent/US10538798B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/13—Protein-histidine kinases (2.7.13)
    • C12Y207/13003—Histidine kinase (2.7.13.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

コリネ型細菌による異種タンパク質の分泌生産を向上させる新規な技術を開発し、異種タンパク質の分泌生産法を提供する。異種タンパク質を分泌生産する能力を有し、且つ、特定の変異を有するphoS遺伝子を保持するように改変されたコリネ型細菌を培養し、異種タンパク質を分泌生産する。
PCT/JP2016/062675 2015-04-24 2016-04-21 タンパク質の分泌生産法 WO2016171224A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1020177034151A KR102619848B1 (ko) 2015-04-24 2016-04-21 단백질의 분비 생산법
EP16783245.0A EP3287523B1 (en) 2015-04-24 2016-04-21 Method for secretory production of protein
CN201680023752.7A CN107532163A (zh) 2015-04-24 2016-04-21 蛋白质的分泌生产方法
AU2016253217A AU2016253217B2 (en) 2015-04-24 2016-04-21 Method for secretory production of protein
DK16783245.0T DK3287523T3 (da) 2015-04-24 2016-04-21 Fremgangsmåde til sekretorisk fremstilling af protein
JP2017514190A JP6741000B2 (ja) 2015-04-24 2016-04-21 タンパク質の分泌生産法
US15/790,495 US10538798B2 (en) 2015-04-24 2017-10-23 Method for secretory production of protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015089046 2015-04-24
JP2015-089046 2015-04-24

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US15/790,495 Continuation US10538798B2 (en) 2015-04-24 2017-10-23 Method for secretory production of protein

Publications (2)

Publication Number Publication Date
WO2016171224A1 WO2016171224A1 (ja) 2016-10-27
WO2016171224A9 true WO2016171224A9 (ja) 2017-09-28

Family

ID=57143158

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2016/062675 WO2016171224A1 (ja) 2015-04-24 2016-04-21 タンパク質の分泌生産法

Country Status (8)

Country Link
US (1) US10538798B2 (ja)
EP (1) EP3287523B1 (ja)
JP (1) JP6741000B2 (ja)
KR (1) KR102619848B1 (ja)
CN (1) CN107532163A (ja)
AU (1) AU2016253217B2 (ja)
DK (1) DK3287523T3 (ja)
WO (1) WO2016171224A1 (ja)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017347016B2 (en) 2016-10-21 2021-09-23 Ajinomoto Co., Inc. Secretory production method for protein
EP3530747A4 (en) 2016-10-21 2020-07-22 Ajinomoto Co., Inc. SECRETORY PROTEIN PRODUCTION PROCESS
WO2019078095A1 (en) 2017-10-16 2019-04-25 Ajinomoto Co., Inc. PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY
CN108330095B (zh) * 2018-03-01 2020-12-29 江南大学 一种积累n-乙酰神经氨酸的重组谷氨酸棒杆菌及其应用
AU2019256065A1 (en) * 2018-04-20 2020-11-26 Ajinomoto Co., Inc. Method for secretory production of protein
CN110511953B (zh) * 2018-08-31 2022-09-30 江南大学 一种适用于谷氨酸棒杆菌的重组表达载体、外源蛋白表达系统、应用和木聚糖酶的制备方法
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
CN112930359A (zh) * 2018-10-25 2021-06-08 味之素株式会社 蛋白质的分泌生产方法
JPWO2020090979A1 (ja) 2018-10-31 2021-09-24 味の素株式会社 抗体に対する親和性物質、切断性部分および反応性基を有する化合物またはその塩
CN111434770B (zh) * 2019-01-11 2022-12-06 华东师范大学 蛋白质谷氨酰胺酶酶原在枯草芽孢杆菌中的表达和自加工
EP4071178A1 (en) 2019-12-06 2022-10-12 Ajinomoto Co., Inc. Method for producing peptide having physiological activity, and peptide comprising short linker
CN117642511A (zh) 2021-07-07 2024-03-01 味之素株式会社 含非天然氨基酸的蛋白质的分泌生产方法
CA3228922A1 (en) * 2021-10-11 2023-04-20 Ajinomoto Co., Inc. Bacterium modified to express heterologous tat protein

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS592689A (ja) * 1982-06-04 1984-01-09 Handai Biseibutsubiyou Kenkyukai 強力な遺伝子発現能を有する新規レプリコンの作成法
US4965197A (en) 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
WO1993003158A1 (fr) 1991-07-30 1993-02-18 Orsan Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries
JPH11169182A (ja) 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
CA2391961C (en) 1999-09-30 2008-12-02 Yoshimi Kikuchi Process for producing transglutaminase
RU2264463C2 (ru) 2001-03-30 2005-11-20 Адзиномото Ко., Инк. Способ секреторной продукции белка
GB0224082D0 (en) * 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
CN100554431C (zh) * 2004-04-20 2009-10-28 味之素株式会社 生产蛋白质的方法
DK1748077T3 (da) 2004-04-20 2013-04-08 Ajinomoto Kk Fremgangsmåde til fremstilling af protein
EP2080769A3 (en) * 2004-07-02 2010-12-01 Metanomics GmbH Process for the production of fine chemicals
CN101675069A (zh) * 2004-07-02 2010-03-17 梅坦诺米克斯有限公司 产生精细化学品的方法
CA2677179C (en) * 2007-01-31 2016-02-16 Dow Global Technologies Inc. Bacterial leader sequences for increased expression
CN103917656B (zh) * 2011-10-25 2016-09-28 味之素株式会社 蛋白质的分泌产生方法
IN2014CN04002A (ja) 2011-11-02 2015-10-23 Ajinomoto Kk
AU2012333451B2 (en) 2011-11-02 2015-05-21 Ajinomoto Co., Inc. Method for secretory production of protein
JPWO2013118544A1 (ja) * 2012-02-08 2015-05-11 味の素株式会社 タンパク質の分泌生産法
US9478749B2 (en) 2013-03-28 2016-10-25 Semiconductor Energy Laboratory Co., Ltd. Anthracene compound, light-emitting element, light-emitting device, electronic appliance, and lighting device

Also Published As

Publication number Publication date
EP3287523A1 (en) 2018-02-28
KR20170140351A (ko) 2017-12-20
CN107532163A (zh) 2018-01-02
AU2016253217A1 (en) 2017-11-23
US10538798B2 (en) 2020-01-21
EP3287523B1 (en) 2022-06-29
AU2016253217B2 (en) 2021-09-02
US20180037918A1 (en) 2018-02-08
WO2016171224A1 (ja) 2016-10-27
DK3287523T3 (da) 2022-07-25
EP3287523A4 (en) 2018-09-05
KR102619848B1 (ko) 2024-01-04
JP6741000B2 (ja) 2020-08-19
JPWO2016171224A1 (ja) 2018-02-22

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