PL3359666T3 - Wczesna izolacja komórek po transfekcji (epic) do wytwarzania środków biologicznych - Google Patents

Wczesna izolacja komórek po transfekcji (epic) do wytwarzania środków biologicznych

Info

Publication number
PL3359666T3
PL3359666T3 PL16788603.5T PL16788603T PL3359666T3 PL 3359666 T3 PL3359666 T3 PL 3359666T3 PL 16788603 T PL16788603 T PL 16788603T PL 3359666 T3 PL3359666 T3 PL 3359666T3
Authority
PL
Poland
Prior art keywords
epic
cells
early post
biologics production
transfection isolation
Prior art date
Application number
PL16788603.5T
Other languages
English (en)
Inventor
Victor R. Cairns
Christine Demaria
Jason VITKO
Original Assignee
Genzyme Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corporation filed Critical Genzyme Corporation
Publication of PL3359666T3 publication Critical patent/PL3359666T3/pl

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/149Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70592CD52

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Epoxy Compounds (AREA)
PL16788603.5T 2015-10-09 2016-10-07 Wczesna izolacja komórek po transfekcji (epic) do wytwarzania środków biologicznych PL3359666T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562239515P 2015-10-09 2015-10-09
PCT/US2016/055918 WO2017062724A1 (en) 2015-10-09 2016-10-07 Early post-transfection isolation of cells (epic) for biologics production

Publications (1)

Publication Number Publication Date
PL3359666T3 true PL3359666T3 (pl) 2025-06-23

Family

ID=57211576

Family Applications (2)

Application Number Title Priority Date Filing Date
PL16788602.7T PL3359665T3 (pl) 2015-10-09 2016-10-07 Ulepszona technologia flare (atenuowana ekspresja reportera z zastosowaniem cytometrii przepływowej) do sortowania z szybkim grupowaniem
PL16788603.5T PL3359666T3 (pl) 2015-10-09 2016-10-07 Wczesna izolacja komórek po transfekcji (epic) do wytwarzania środków biologicznych

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PL16788602.7T PL3359665T3 (pl) 2015-10-09 2016-10-07 Ulepszona technologia flare (atenuowana ekspresja reportera z zastosowaniem cytometrii przepływowej) do sortowania z szybkim grupowaniem

Country Status (12)

Country Link
US (4) US10317329B2 (pl)
EP (4) EP4567425A3 (pl)
CN (3) CN108368505B (pl)
AU (3) AU2016335699A1 (pl)
CA (2) CA3001238C (pl)
ES (2) ES3024209T3 (pl)
HU (2) HUE069884T2 (pl)
IL (4) IL297805A (pl)
MX (1) MX2018004366A (pl)
PL (2) PL3359665T3 (pl)
SG (3) SG11201802823VA (pl)
WO (2) WO2017062724A1 (pl)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10577627B2 (en) 2014-06-09 2020-03-03 Voyager Therapeutics, Inc. Chimeric capsids
CA2966620A1 (en) 2014-11-05 2016-05-12 Voyager Therapeutics, Inc. Aadc polynucleotides for the treatment of parkinson's disease
CA2967367C (en) 2014-11-14 2023-05-16 Voyager Therapeutics, Inc. Compositions and methods of treating amyotrophic lateral sclerosis (als)
CA2975583A1 (en) 2014-11-14 2016-05-19 Voyager Therapeutics, Inc. Modulatory polynucleotides
HK1245326A1 (zh) 2014-12-12 2018-08-24 Voyager Therapeutics, Inc. 用於生产scaav的组合物和方法
HUE069884T2 (hu) 2015-10-09 2025-04-28 Genzyme Corp Javított flare (áramlási citometriával csillapított reporter expresszió) technológia gyors tömeges válogatáshoz
WO2017189959A1 (en) 2016-04-29 2017-11-02 Voyager Therapeutics, Inc. Compositions for the treatment of disease
US11299751B2 (en) 2016-04-29 2022-04-12 Voyager Therapeutics, Inc. Compositions for the treatment of disease
KR20220108216A (ko) 2016-05-18 2022-08-02 보이저 테라퓨틱스, 인크. 헌팅톤 질환을 치료하기 위한 조성물 및 방법
KR20240056729A (ko) 2016-05-18 2024-04-30 보이저 테라퓨틱스, 인크. 조절성 폴리뉴클레오티드
CL2016001661A1 (es) * 2016-06-29 2017-03-03 Univ Chile Promotor hibrido de ß-actina (de cricetulus griseus) y citomegalovirus (cmv), con región rica en dinucleótidos, citosina-guanina, vectores, líneas celulares, procedimiento para producir proteínas recombinantes.
AU2017321488B2 (en) 2016-08-30 2022-10-20 The Regents Of The University Of California Methods for biomedical targeting and delivery and devices and systems for practicing the same
CN110050065B (zh) 2016-10-07 2024-05-07 建新公司 用于生物制品生产的细胞的早期转染后分离(epic)
SG11201909868YA (en) 2017-05-05 2019-11-28 Voyager Therapeutics Inc Compositions and methods of treating huntington's disease
EP3618839A4 (en) 2017-05-05 2021-06-09 Voyager Therapeutics, Inc. Compositions and methods of treating amyotrophic lateral sclerosis (als)
JOP20190269A1 (ar) 2017-06-15 2019-11-20 Voyager Therapeutics Inc بولي نوكليوتيدات aadc لعلاج مرض باركنسون
WO2019018342A1 (en) 2017-07-17 2019-01-24 Voyager Therapeutics, Inc. NETWORK EQUIPMENT TRACK GUIDE SYSTEM
AU2018338728B2 (en) 2017-09-29 2025-01-02 Centre National De La Recherche Scientifique (Cnrs) Rescue of central and peripheral neurological phenotype of Friedreich's Ataxia by intravenous delivery
EP3697908A1 (en) 2017-10-16 2020-08-26 Voyager Therapeutics, Inc. Treatment of amyotrophic lateral sclerosis (als)
WO2019079240A1 (en) 2017-10-16 2019-04-25 Voyager Therapeutics, Inc. TREATMENT OF AMYOTROPHIC LATERAL SCLEROSIS (ALS)
MX2020012077A (es) 2018-05-15 2021-03-09 Voyager Therapeutics Inc Composiciones y metodos para el tratamiento de la enfermedad de parkinson.
EP3856762A1 (en) 2018-09-28 2021-08-04 Voyager Therapeutics, Inc. Frataxin expression constructs having engineered promoters and methods of use thereof
CA3199001A1 (en) 2020-11-19 2022-05-27 Victor R. Cairns Flow cytometry attenuated reporter expression (flare) multiple reporter system and methods of use thereof
US20250281605A1 (en) 2024-03-08 2025-09-11 Sanofi Antigenic Epstein Barr Virus Polypeptides

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
EP0484411A4 (en) 1989-07-24 1992-06-17 Seragen, Inc. Prevention of internal initiation
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
WO1994026087A2 (en) 1993-05-14 1994-11-24 Connor Kim C O Recombinant protein production and insect cell culture and process
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US7252989B1 (en) 1994-04-04 2007-08-07 Board Of Regents, The University Of Texas System Adenovirus supervector system
US5955349A (en) 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5736383A (en) 1996-08-26 1998-04-07 Zymogenetics, Inc. Preparation of Pichia methanolica auxotrophic mutants
WO1998037189A1 (en) 1997-02-25 1998-08-27 Qbi Enterprises Ltd. Ires sequences with high translational efficiency and expression vectors containing the sequence
US7001733B1 (en) * 1998-05-12 2006-02-21 Rigel Pharmaceuticals, Inc. Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
CA2385102A1 (en) * 1999-07-12 2001-01-18 Genentech, Inc. Expression vectors and methods
US20050005310A1 (en) * 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
AU2001236624A1 (en) 2000-02-02 2001-08-14 Iconix Pharmaceuticals, Inc. Rapid, parallel identification of cell lines
WO2002070710A1 (en) 2001-03-01 2002-09-12 University Of California Method to identify ires elements
DE10143238A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
WO2003099996A2 (en) 2002-05-22 2003-12-04 Biogen Idec Ma Inc. Detection of secreted polypeptides
US7119187B2 (en) 2002-07-09 2006-10-10 National Health Research Institutes Internal ribosome entry site of the labial gene for protein expression
GB0216648D0 (en) 2002-07-18 2002-08-28 Lonza Biologics Plc Method of expressing recombinant protein in CHO cells
WO2004009788A2 (en) * 2002-07-19 2004-01-29 Diversa Corporation Fluorescent proteins, nucleic acids encoding them and methods for making and using them
US7384744B2 (en) * 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
US7344886B2 (en) 2002-11-29 2008-03-18 Boehringer Ingelheim Pharma Gmbh & Co., Kg Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product
AU2003300932A1 (en) 2002-12-16 2004-07-29 Wayne State University Bioactive peptides and unique ires elements from myelin proteolipid protein plp/dm20
EP3346010A1 (en) 2003-06-24 2018-07-11 Genzyme Corporation Novel beta-actin and rps21 promoters and uses thereof
US7776584B2 (en) 2003-08-01 2010-08-17 Genetix Limited Animal cell colony picking apparatus and method
WO2005094886A1 (ja) 2004-03-31 2005-10-13 Kirin Beer Kabushiki Kaisha Gpiアンカー蛋白質アゴニストによる調節性t細胞分化誘導・増殖方法およびそのための医薬組成物
US7078179B2 (en) 2004-05-04 2006-07-18 Newlink Genetics Corporation Selectable gene marker system based on expression of N-acetyllactosaminide 3-α galactosyltransferase
WO2006023248A2 (en) * 2004-07-28 2006-03-02 The Trustees Of Columbia University In The City Of New York Processes for producing and crystallizing g-protein coupled receptors
EP1809750B1 (en) 2004-11-08 2012-03-21 ChromaGenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
CA2588936A1 (en) 2004-12-14 2006-06-22 F. Hoffmann-La Roche Ag Method for improved selection of rnai transfectants
CA2648778A1 (en) 2006-04-10 2007-10-18 The Regents Of The University Of California Method for culturing cells on removable pallets for subsequent cell expansion and analysis
PL2064337T3 (pl) * 2006-09-20 2012-12-31 Genzyme Corp Oparty na FACS i białku reporterowym układ do wysokowydajnego opracowywania białek terapeutycznych
EP2644199B1 (en) 2007-03-05 2017-10-11 NewSouth Innovations Pty Limited Methods for detecting and modulating the sensitivity of tumour cells to anti-mitotic agents
US9534246B2 (en) * 2010-07-01 2017-01-03 Glaxo Group Limited Method for selecting high producing cell lines
EP3520821A1 (en) * 2012-04-02 2019-08-07 Moderna Therapeutics, Inc. Modified polynucleotides for the production of biologics and proteins associated with human disease
EP2700713B1 (en) * 2012-08-21 2016-07-13 Miltenyi Biotec GmbH Screening and enrichment system for protein expression in eukaryotic cells using a tricistronic expression cassette
WO2014141037A1 (en) 2013-03-11 2014-09-18 Novartis Ag Method of screening cell clones
LT3027646T (lt) * 2013-07-31 2018-09-25 Novartis Ag Nauji atrankos vektoriai ir eukariotinių ląstelių šeimininkių atrankos būdai
HUE069884T2 (hu) 2015-10-09 2025-04-28 Genzyme Corp Javított flare (áramlási citometriával csillapított reporter expresszió) technológia gyors tömeges válogatáshoz
CN110050065B (zh) 2016-10-07 2024-05-07 建新公司 用于生物制品生产的细胞的早期转染后分离(epic)

Also Published As

Publication number Publication date
HUE069884T2 (hu) 2025-04-28
AU2016335699A1 (en) 2018-05-24
WO2017062722A2 (en) 2017-04-13
IL258538B2 (en) 2023-04-01
EP3359665A2 (en) 2018-08-15
SG11201802829YA (en) 2018-05-30
CA3001364A1 (en) 2017-04-13
CA3001238A1 (en) 2017-04-13
CN108368504A (zh) 2018-08-03
EP3359666B1 (en) 2025-02-19
EP3359665C0 (en) 2024-11-13
IL258537B2 (en) 2024-05-01
EP3359666C0 (en) 2025-02-19
IL258538B (en) 2022-12-01
AU2022275507B2 (en) 2025-06-05
US20170227441A1 (en) 2017-08-10
IL297805A (en) 2022-12-01
EP4530350A3 (en) 2025-06-25
US20230304913A1 (en) 2023-09-28
ES3024209T3 (en) 2025-06-04
US20200018682A1 (en) 2020-01-16
IL309289A (en) 2024-02-01
MX2018004366A (es) 2018-08-01
AU2022275507A1 (en) 2023-02-02
CN108368504B (zh) 2022-07-19
US10317329B2 (en) 2019-06-11
HK1259493A1 (en) 2019-11-29
EP4567425A3 (en) 2025-10-08
ES3008383T3 (en) 2025-03-24
HUE071193T2 (hu) 2025-08-28
CN108368505A (zh) 2018-08-03
IL258537A (en) 2018-05-31
WO2017062724A1 (en) 2017-04-13
EP3359666A1 (en) 2018-08-15
US11635363B2 (en) 2023-04-25
CN116660127A (zh) 2023-08-29
IL258538A (en) 2018-05-31
IL258537B1 (en) 2024-01-01
EP4567425A2 (en) 2025-06-11
US20170121734A1 (en) 2017-05-04
WO2017062722A3 (en) 2017-05-26
EP4530350A2 (en) 2025-04-02
EP3359665B1 (en) 2024-11-13
CN108368505B (zh) 2023-02-21
AU2025226783A1 (en) 2025-09-25
CA3001238C (en) 2024-06-25
SG11201802823VA (en) 2018-05-30
SG10202111233SA (en) 2021-11-29
PL3359665T3 (pl) 2025-04-14

Similar Documents

Publication Publication Date Title
IL258538A (en) Early isolation after the infection of cells (epic) for the production of biological material
EP3345990A4 (en) SYSTEM FOR THE PRODUCTION OF PLURIPOTENTAL STEM CELLS
SG11201706636PA (en) Single-cell nucleic acids for high-throughput studies
EP3177904A4 (en) Platelet-targeted microfluidic isolation of cells
LT3365107T (lt) Ląstelių kultūra
PL3119575T3 (pl) Sposób wytwarzania izolacyjnego środnika
EP3140425A4 (en) Methods of linearly amplifying whole genome of a single cell
ZA201707477B (en) Methods for assesing the purity of a mesenchymal stem cells preparation
PL3140392T3 (pl) Produkcja białek heteromultimerycznych z wykorzystaniem komórek ssaków
GB2525949B (en) Process for the production of dialkyl succinate
IL261999A (en) Cell culture process
GB2525950B (en) Process for the production of dialkyl succinate
GB201420428D0 (en) Methods for nucleic acid isolation
EP3233814A4 (en) Novel process for the preparation of ranolazine
EP3196304A4 (en) Method for improved high secretory production of proteins
PT3362405T (pt) Processo e unidade de produção para a produção de metanol
GB201504459D0 (en) Isolation of DNA
SG10202103545PA (en) Early post-transfection isolation of cells (epic) for biologics production
SG11201705939RA (en) A contractile cellular construct for cell culture
IL257814A (en) Method for making praziquantal
SG11201705095VA (en) Process for the manufacture of solar cells
GB201408753D0 (en) Isolation of adipose derived cells
SG11201609269TA (en) Method of culturing cells
EP3378945A4 (en) Method for distinguishing particular cells from mixture of heterogeneous cells
HK1238333A1 (en) Platelet-targeted microfluidic isolation of cells