PH12021552730A1 - Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof - Google Patents
Kaurenoic acid 13-hydroxylase (kah) variants and uses thereofInfo
- Publication number
- PH12021552730A1 PH12021552730A1 PH1/2021/552730A PH12021552730A PH12021552730A1 PH 12021552730 A1 PH12021552730 A1 PH 12021552730A1 PH 12021552730 A PH12021552730 A PH 12021552730A PH 12021552730 A1 PH12021552730 A1 PH 12021552730A1
- Authority
- PH
- Philippines
- Prior art keywords
- kah
- rebaudioside
- steviol glycosides
- host cells
- variants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962842810P | 2019-05-03 | 2019-05-03 | |
| PCT/US2020/031171 WO2020227148A1 (en) | 2019-05-03 | 2020-05-01 | Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PH12021552730A1 true PH12021552730A1 (en) | 2022-11-14 |
Family
ID=73051309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PH1/2021/552730A PH12021552730A1 (en) | 2019-05-03 | 2020-05-01 | Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US12467039B2 (enExample) |
| EP (1) | EP3962931A4 (enExample) |
| JP (1) | JP2022530986A (enExample) |
| KR (1) | KR20220005021A (enExample) |
| CN (1) | CN113906045A (enExample) |
| AU (1) | AU2020268897A1 (enExample) |
| BR (1) | BR112021022010A2 (enExample) |
| CA (1) | CA3134729A1 (enExample) |
| MX (1) | MX2021013124A (enExample) |
| PH (1) | PH12021552730A1 (enExample) |
| SG (1) | SG11202111698SA (enExample) |
| WO (1) | WO2020227148A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN122214168B (zh) * | 2026-05-18 | 2026-07-21 | 四川盈嘉合生科技有限公司 | 一种全发酵生产甜菊糖苷的酵母工程菌及其应用 |
| CN122188954B (zh) * | 2026-05-18 | 2026-07-17 | 四川盈嘉合生科技有限公司 | 贝壳杉烯酸13-羟化酶及在全发酵产甜菊糖苷中的应用 |
| CN122405577A (zh) * | 2026-06-16 | 2026-07-17 | 深圳希吉亚生物技术有限公司 | 二氢叶酸还原酶突变体及其在合成5-甲基四氢叶酸5-mthf中的应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2742142B2 (en) | 2011-08-08 | 2020-03-25 | Evolva SA | Recombinant production of steviol glycosides |
| US10017804B2 (en) | 2013-02-11 | 2018-07-10 | Evolva Sa | Efficient production of steviol glycosides in recombinant hosts |
| CN104928263B (zh) | 2014-03-20 | 2018-03-23 | 中国科学院上海生命科学研究院 | 一种新的贝壳烯酸‑13α‑羟化酶、其编码基因及其应用 |
| JP2017528134A (ja) | 2014-09-09 | 2017-09-28 | エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． | 組み換え宿主におけるステビオールグリコシドの生産 |
| US10463062B2 (en) | 2014-11-05 | 2019-11-05 | Manus Bio Inc. | Microbial production of steviol glycosides |
| BR112017016338A2 (en) | 2015-01-30 | 2018-03-27 | Evolva Sa | production of steviol glycosides in recombinant hosts |
| CN107614677A (zh) | 2015-05-29 | 2018-01-19 | 嘉吉公司 | 用于使用多阶段进料生产甜菊醇糖苷的发酵方法 |
| US10760085B2 (en) | 2015-10-05 | 2020-09-01 | Dsm Ip Assets B.V. | Kaurenoic acid hydroxylases |
| US20190062796A1 (en) | 2015-12-10 | 2019-02-28 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| WO2018031955A2 (en) | 2016-08-12 | 2018-02-15 | Amyris, Inc. | Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides |
| WO2018211032A1 (en) | 2017-05-17 | 2018-11-22 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CA3114953A1 (en) | 2018-10-15 | 2020-04-23 | Amyris, Inc. | Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides |
-
2020
- 2020-05-01 SG SG11202111698SA patent/SG11202111698SA/en unknown
- 2020-05-01 CA CA3134729A patent/CA3134729A1/en active Pending
- 2020-05-01 BR BR112021022010A patent/BR112021022010A2/pt unknown
- 2020-05-01 MX MX2021013124A patent/MX2021013124A/es unknown
- 2020-05-01 PH PH1/2021/552730A patent/PH12021552730A1/en unknown
- 2020-05-01 US US17/608,437 patent/US12467039B2/en active Active
- 2020-05-01 KR KR1020217037649A patent/KR20220005021A/ko not_active Ceased
- 2020-05-01 EP EP20802800.1A patent/EP3962931A4/en active Pending
- 2020-05-01 WO PCT/US2020/031171 patent/WO2020227148A1/en not_active Ceased
- 2020-05-01 JP JP2021564945A patent/JP2022530986A/ja active Pending
- 2020-05-01 AU AU2020268897A patent/AU2020268897A1/en not_active Abandoned
- 2020-05-01 CN CN202080040694.5A patent/CN113906045A/zh active Pending
-
2025
- 2025-06-30 US US19/254,544 patent/US20260062683A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| BR112021022010A2 (pt) | 2021-12-21 |
| AU2020268897A1 (en) | 2021-11-18 |
| CN113906045A (zh) | 2022-01-07 |
| KR20220005021A (ko) | 2022-01-12 |
| WO2020227148A9 (en) | 2021-07-29 |
| WO2020227148A1 (en) | 2020-11-12 |
| JP2022530986A (ja) | 2022-07-05 |
| MX2021013124A (es) | 2022-01-24 |
| EP3962931A1 (en) | 2022-03-09 |
| EP3962931A4 (en) | 2023-06-14 |
| US12467039B2 (en) | 2025-11-11 |
| US20260062683A1 (en) | 2026-03-05 |
| US20220282228A1 (en) | 2022-09-08 |
| SG11202111698SA (en) | 2021-11-29 |
| CA3134729A1 (en) | 2020-11-12 |
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