PE20220564A1 - Metodo para la caracterizacion de polipeptidos que se unen a complejos de peptido.mhc - Google Patents

Metodo para la caracterizacion de polipeptidos que se unen a complejos de peptido.mhc

Info

Publication number
PE20220564A1
PE20220564A1 PE2022000231A PE2022000231A PE20220564A1 PE 20220564 A1 PE20220564 A1 PE 20220564A1 PE 2022000231 A PE2022000231 A PE 2022000231A PE 2022000231 A PE2022000231 A PE 2022000231A PE 20220564 A1 PE20220564 A1 PE 20220564A1
Authority
PE
Peru
Prior art keywords
peptide
mhc
binding
bind
complex
Prior art date
Application number
PE2022000231A
Other languages
English (en)
Inventor
Heiko Schuster
Meike Hutt
Toni Weinschenk
Sebastian Bunk
Oliver Schoor
Linus Backert
Martin Hofmann
Jens Fritsche
Felix Unverdorben
Gisela Schimmack
Florian Schworer
Original Assignee
Immatics Biotechnologies Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immatics Biotechnologies Gmbh filed Critical Immatics Biotechnologies Gmbh
Publication of PE20220564A1 publication Critical patent/PE20220564A1/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in eptitope analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70539—MHC-molecules, e.g. HLA-molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invencion concierne a un metodo para la caracterizacion de polipeptidos que se unen a complejos de peptido:MHC, p. ej. mediante espectrometria de masas y un analisis del espacio peptidico reconocido, es decir, con el fin de identificar peptidos que puedan ser unidos en el contexto de su presentacion por el MHC, y aquellos que no puedan serlo. Tambien se refiere a un metodo para caracterizar la union de una molecula polipeptidica, que comprende al menos un dominio definido de union a peptido ("polipeptido de union a pMHC") a un peptido ("peptido diana") de un complejo de peptido:MHC, el cual comprende: a) facilitar una muestra que comprenda al menos un complejo de peptido:MHC que ha de ser analizada, b) la puesta en contacto de dicha muestra con dicha molecula polipeptidica, ("polipeptido de union a pMHC") y posibilitar que al menos un citado dominio de union a peptido de dicha molecula polipeptidica se una a como minimo un citado complejo de peptido:MHC, c) aislamiento como minimo de un citado complejo de peptido:MHC unido a, como minimo, un citado dominio de union a peptido, y d) identificacion de dicho peptido que esta formando parte de como minimo un complejo de peptido:MHC citado que se ha aislado en el paso c), y de ese modo identificacion de la union de la citada molecula polipeptidica a dicho peptido de como minimo un complejo de peptido:MHC citado
PE2022000231A 2019-08-13 2020-08-12 Metodo para la caracterizacion de polipeptidos que se unen a complejos de peptido.mhc PE20220564A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962886225P 2019-08-13 2019-08-13
DE102019121834 2019-08-13
PCT/EP2020/072674 WO2021028503A1 (en) 2019-08-13 2020-08-12 Method for the characterization of peptide:mhc binding polypeptides

Publications (1)

Publication Number Publication Date
PE20220564A1 true PE20220564A1 (es) 2022-04-13

Family

ID=72088088

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2022000231A PE20220564A1 (es) 2019-08-13 2020-08-12 Metodo para la caracterizacion de polipeptidos que se unen a complejos de peptido.mhc

Country Status (16)

Country Link
US (1) US20210048442A1 (es)
EP (1) EP4014043A1 (es)
JP (1) JP2022547794A (es)
KR (1) KR20220044485A (es)
CN (1) CN114375397A (es)
AU (1) AU2020330717A1 (es)
BR (1) BR112022002699A2 (es)
CA (1) CA3150546A1 (es)
CL (1) CL2022000352A1 (es)
CO (1) CO2022001531A2 (es)
CR (1) CR20220065A (es)
IL (1) IL289209A (es)
MX (1) MX2022001869A (es)
PE (1) PE20220564A1 (es)
TW (1) TW202120925A (es)
WO (1) WO2021028503A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102375218B1 (ko) 2016-12-08 2022-03-17 이매틱스 바이오테크놀로지스 게엠베하 T 세포 수용체 및 이를 사용하는 면역 요법
WO2024033332A1 (en) 2022-08-08 2024-02-15 Immatics Biotechnologies Gmbh Guided positional scanning method

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1247095B1 (en) * 1999-12-06 2008-10-15 Board Of Trustees Of The University Of Illinois High affinity tcr proteins and methods
EP1714157A2 (en) 2004-01-28 2006-10-25 Immatics Biotechnologies GmbH Method for identifying and quantifying of tumour-associated peptides
GB201006360D0 (en) 2010-04-16 2010-06-02 Immatics Biotechnologies Gmbh Method for differentially quantifying naturally processed HLA-restricted peptides for cancer, autoimmune and infectious diseases immunotherapy development
EA201390011A1 (ru) 2010-07-28 2013-07-30 Иммьюнокор Лтд. Т-клеточные рецепторы
WO2012104843A1 (en) 2011-02-06 2012-08-09 Yeda Research And Development Co.Ltd. At The Weizmann Institute Of Science Affinity maturated t cell receptors and use thereof
GB201309469D0 (en) * 2013-05-28 2013-07-10 Imcyse Sa Detection of CD4+ T lymphocytes
GB201506423D0 (en) 2015-04-15 2015-05-27 Tc Biopharm Ltd Gamma delta T cells and uses thereof
GB201423361D0 (en) 2014-12-30 2015-02-11 Immatics Biotechnologies Gmbh Method for the absolute Quantification of naturally processed HLA-Restricted cancer peptides
NL2014935B1 (en) 2015-06-08 2017-02-03 Applied Immune Tech Ltd T cell receptor like antibodies having fine specificity.
GB201520592D0 (en) * 2015-11-23 2016-01-06 Immunocore Ltd & Adaptimmune Ltd Peptides
WO2017115798A1 (ja) * 2015-12-28 2017-07-06 北海道公立大学法人 札幌医科大学 腫瘍抗原ペプチド
CN116239669A (zh) 2016-04-08 2023-06-09 艾达普特免疫有限公司 T细胞受体
WO2018053374A2 (en) * 2016-09-16 2018-03-22 Memorial Sloan Kettering Cancer Center Methods and compositions for t-cell epitope screening
DE102018122546B3 (de) * 2018-09-14 2019-12-05 Immatics Biotechnologies Gmbh Verfahren zum Hochdurchsatz-Peptid-MHC-Affinitätsscreening für TCR-Liganden

Also Published As

Publication number Publication date
JP2022547794A (ja) 2022-11-16
IL289209A (en) 2022-02-01
EP4014043A1 (en) 2022-06-22
WO2021028503A8 (en) 2022-04-07
MX2022001869A (es) 2022-03-11
KR20220044485A (ko) 2022-04-08
BR112022002699A2 (pt) 2022-05-31
CO2022001531A2 (es) 2022-06-21
CN114375397A (zh) 2022-04-19
TW202120925A (zh) 2021-06-01
CA3150546A1 (en) 2021-02-18
WO2021028503A1 (en) 2021-02-18
US20210048442A1 (en) 2021-02-18
AU2020330717A1 (en) 2022-02-03
CR20220065A (es) 2022-05-04
CL2022000352A1 (es) 2022-09-23

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