BR112013025629A2 - padrão quantitativo para espectrometria de massa de proteínas - Google Patents
padrão quantitativo para espectrometria de massa de proteínasInfo
- Publication number
- BR112013025629A2 BR112013025629A2 BR112013025629A BR112013025629A BR112013025629A2 BR 112013025629 A2 BR112013025629 A2 BR 112013025629A2 BR 112013025629 A BR112013025629 A BR 112013025629A BR 112013025629 A BR112013025629 A BR 112013025629A BR 112013025629 A2 BR112013025629 A2 BR 112013025629A2
- Authority
- BR
- Brazil
- Prior art keywords
- polypeptide
- hand
- absolute amount
- target
- sample
- Prior art date
Links
- 238000004949 mass spectrometry Methods 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 16
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 16
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 16
- 230000004927 fusion Effects 0.000 abstract 5
- 238000002372 labelling Methods 0.000 abstract 3
- 230000029087 digestion Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000002797 proteolythic effect Effects 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000001819 mass spectrum Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/05—Isotopically modified compounds, e.g. labelled
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
resumo patente de invenção: "padrão quantitativo para espectrometria de massa de proteínas". a presente invenção refere-se a um método de determinação da quantidade absoluta de um polipeptídeo alvo em uma amostra, o referido método compreendendo as seguintes etapas: (a) adicionar (aa) um polipeptídeo de fusão à referida amostra, o referido polipeptídeo de fusão compreendendo (i) pelo menos um sequência de marcação e (ii) uma subsequência do polipeptídeo alvo; e (ab) uma quantidade absoluta conhecida de um polipeptídeo de marcação que compreende ou consiste na referida sequência de marcação de acordo com (aa) à referida amostra, em que o referido polipeptídeo de fusão tem, por um lado, massa alterada quando comparado com o referido polipeptídeo alvo e o referido polipeptídeo de marcação, por outro lado, por exemplo, o referido polipeptídeo de fusão por um lado e o referido polipeptídeo alvo e o referido polipeptídeo de marcação por outro lado, são marcados isotopicamente de maneira diferente; (b) realizar uma digestão proteolítica da mistura obtida na etapa (a); (c) sujeitar o resultado da digestão proteolítica da etapa (b), opcionalmente após cromatografia, à análise por espectrometria de massa; e (d) determinar a quantidade absoluta do referido polipeptídeo alvo a partir (i) das intensidades de pico no espectro de massa adquirido na etapa (c) do referido polipeptídeo de fusão, do referido polipeptídeo de marcação e do referido polipeptídeo alvo e (ii) da referida quantidade absoluta conhecida do referido polipeptídeo de marcação.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161471528P | 2011-04-04 | 2011-04-04 | |
| US201161471534P | 2011-04-04 | 2011-04-04 | |
| EP11002794A EP2508537A1 (en) | 2011-04-04 | 2011-04-04 | Quantitative standard for mass spectrometry of proteins |
| PCT/EP2012/056234 WO2012136737A1 (en) | 2011-04-04 | 2012-04-04 | Quantitative standard for mass spectrometry of proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112013025629A2 true BR112013025629A2 (pt) | 2016-11-29 |
Family
ID=44484002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112013025629A BR112013025629A2 (pt) | 2011-04-04 | 2012-04-04 | padrão quantitativo para espectrometria de massa de proteínas |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9063149B2 (pt) |
| EP (3) | EP2508537A1 (pt) |
| JP (1) | JP2014520247A (pt) |
| CN (1) | CN103582652A (pt) |
| AU (1) | AU2012238641A1 (pt) |
| BR (1) | BR112013025629A2 (pt) |
| CA (1) | CA2831998A1 (pt) |
| DK (2) | DK2694556T3 (pt) |
| ES (2) | ES2602033T3 (pt) |
| PL (2) | PL2767834T3 (pt) |
| WO (1) | WO2012136737A1 (pt) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2956768B1 (en) * | 2013-02-13 | 2023-10-18 | Promega Corporation | Method for assessing performance of an instrument with liquid chromatography and mass spectrometry functionalities |
| CN106033087B (zh) * | 2015-03-18 | 2018-05-18 | 王峥 | 内置性标准曲线检测物质分子数之方法系统 |
| EP3109639A1 (en) * | 2015-06-26 | 2016-12-28 | Atlas Antibodies AB | Mass spectrometry quantification |
| US11698378B2 (en) * | 2015-09-25 | 2023-07-11 | Children's Medical Center Corporation | Methods and compositions for tauopathy diagnosis and treatment |
| CN108473529B (zh) * | 2015-11-10 | 2023-04-18 | 伦斯勒中心转化研究公司 | 检测和治疗肺高压的方法 |
| RU2729116C2 (ru) | 2015-12-16 | 2020-08-04 | Гритстоун Онколоджи, Инк. | Идентификация, производство и применение неоантигенов |
| SG11201805536VA (en) * | 2015-12-29 | 2018-07-30 | Sanofi Sa | Methods for characterizing compositions comprising peanut antigens |
| CN106841368B (zh) * | 2016-10-19 | 2019-05-24 | 苏州大学 | 一种活细胞内蛋白质棕榈酰化率的变化率的测定方法 |
| AU2017359284A1 (en) * | 2016-11-14 | 2019-05-30 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Low energy cleavable mass tag for quantitative proteomics |
| KR20200087143A (ko) | 2017-10-10 | 2020-07-20 | 그릿스톤 온콜로지, 인코포레이티드 | 핫스팟을 이용한 신생항원 동정 |
| JP2021503897A (ja) | 2017-11-22 | 2021-02-15 | グリットストーン オンコロジー インコーポレイテッド | 新生抗原のためのジャンクションエピトープ提示の低減 |
| EP3811081A1 (en) * | 2018-06-21 | 2021-04-28 | Universiteit Utrecht Holding B.V. | Method for monitoring kinase activity in a sample |
| EP3736574A1 (en) | 2019-05-07 | 2020-11-11 | Atlas Antibodies AB | A formulation comprising an isotope labeled fusion polypeptide |
| CN113774074A (zh) * | 2020-06-05 | 2021-12-10 | 复旦大学 | 一种基于肽段的靶向蛋白质组精确定量方法 |
| CN112786105B (zh) * | 2020-12-07 | 2024-05-07 | 中山大学附属第五医院 | 一种宏蛋白质组挖掘方法及其在获取肠道微生物蛋白水解特征中的应用 |
| CN117912591B (zh) * | 2024-03-19 | 2024-05-31 | 鲁东大学 | 一种基于深度对比学习的激酶药物相互作用预测方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2726471B1 (fr) * | 1994-11-07 | 1997-01-31 | Pf Medicament | Procede pour ameliorer l'immunogenicite d'un compose immunogene ou d'un haptene et application a la preparation de vaccins |
| US20090087878A9 (en) * | 1999-05-06 | 2009-04-02 | La Rosa Thomas J | Nucleic acid molecules associated with plants |
| US7166436B2 (en) * | 2001-01-26 | 2007-01-23 | Syngenta Participations, Ag | Differential labeling for quantitative analysis of complex protein mixtures |
| DE60239962D1 (de) * | 2001-08-14 | 2011-06-16 | Harvard College | Absolute quantifizierung von proteinen und modifizierten formen davon mittels mehrstufiger massenspektrometrie |
| US7655433B2 (en) * | 2002-06-04 | 2010-02-02 | The Institute For Systems Biology | Methods for high-throughput and quantitative proteome analysis |
| EP1736480B9 (en) * | 2005-06-02 | 2009-03-04 | Polyquant GmbH | Artificial protein, method for absolute quantification of proteins and uses thereof |
| JP2010530962A (ja) * | 2007-06-12 | 2010-09-16 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー | 血漿においてタンパク質を検出する方法 |
-
2011
- 2011-04-04 EP EP11002794A patent/EP2508537A1/en not_active Withdrawn
-
2012
- 2012-04-04 PL PL14157821.1T patent/PL2767834T3/pl unknown
- 2012-04-04 PL PL12711680T patent/PL2694556T3/pl unknown
- 2012-04-04 CA CA2831998A patent/CA2831998A1/en not_active Abandoned
- 2012-04-04 BR BR112013025629A patent/BR112013025629A2/pt not_active IP Right Cessation
- 2012-04-04 US US14/009,801 patent/US9063149B2/en active Active
- 2012-04-04 WO PCT/EP2012/056234 patent/WO2012136737A1/en active Application Filing
- 2012-04-04 JP JP2014503142A patent/JP2014520247A/ja active Pending
- 2012-04-04 EP EP14157821.1A patent/EP2767834B1/en active Active
- 2012-04-04 ES ES12711680.4T patent/ES2602033T3/es active Active
- 2012-04-04 ES ES14157821.1T patent/ES2589530T3/es active Active
- 2012-04-04 EP EP12711680.4A patent/EP2694556B1/en active Active
- 2012-04-04 DK DK12711680.4T patent/DK2694556T3/en active
- 2012-04-04 AU AU2012238641A patent/AU2012238641A1/en not_active Abandoned
- 2012-04-04 CN CN201280027258.XA patent/CN103582652A/zh active Pending
- 2012-04-04 DK DK14157821.1T patent/DK2767834T3/en active
Also Published As
| Publication number | Publication date |
|---|---|
| PL2767834T3 (pl) | 2016-12-30 |
| DK2694556T3 (en) | 2016-11-28 |
| EP2508537A1 (en) | 2012-10-10 |
| EP2767834A3 (en) | 2014-10-01 |
| EP2767834A2 (en) | 2014-08-20 |
| EP2694556A1 (en) | 2014-02-12 |
| ES2589530T3 (es) | 2016-11-15 |
| DK2767834T3 (en) | 2016-09-05 |
| CA2831998A1 (en) | 2012-10-11 |
| AU2012238641A1 (en) | 2013-10-10 |
| PL2694556T3 (pl) | 2017-03-31 |
| CN103582652A (zh) | 2014-02-12 |
| ES2602033T3 (es) | 2017-02-17 |
| US20140072991A1 (en) | 2014-03-13 |
| EP2694556B1 (en) | 2016-08-24 |
| US9063149B2 (en) | 2015-06-23 |
| WO2012136737A1 (en) | 2012-10-11 |
| JP2014520247A (ja) | 2014-08-21 |
| EP2767834B1 (en) | 2016-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112013025629A2 (pt) | padrão quantitativo para espectrometria de massa de proteínas | |
| BR112013031284A2 (pt) | composições, métodos, e kits para quantificar analitos alvo em uma amostra | |
| Simpson et al. | Acetone precipitation of proteins and the modification of peptides | |
| Mora et al. | Proteomic identification of antioxidant peptides from 400 to 2500 Da generated in Spanish dry-cured ham contained in a size-exclusion chromatography fraction | |
| Cole et al. | Electron transfer dissociation (ETD) of peptides containing intrachain disulfide bonds | |
| BR112012008537A2 (pt) | processo de caracterização de pelo menos um micro-organismo por espectrometria de massa | |
| Park et al. | Mass spectrometry-based N-linked glycomic profiling as a means for tracking pancreatic cancer metastasis | |
| BR112017017025A2 (pt) | métodos e composições para analizar componentes celulares | |
| BR112014010955A2 (pt) | sistema e método para processar amostras | |
| EP4249605A3 (en) | Methods for analyte detection | |
| BRPI0516674A (pt) | método de análise de mal de alzheimer e reagente de diagnóstico | |
| WO2018072862A8 (de) | Verfahren zum auswerten von daten einer massenspektrometrie und massenspektrometrisches verfahren sowie ein maldi tof massenspektrometer | |
| SG127861A1 (en) | in situ biomarker identification | |
| Yuan et al. | Thiol metabolomics of endothelial cells using capillary liquid chromatography mass spectrometry with isotope coded affinity tags | |
| BR112019024602A2 (pt) | ensaio para doença associada à célula de plasma | |
| BR112015005887A2 (pt) | quantificação de tiroglobulina por espectroscopia de massa | |
| BRPI0907656A2 (pt) | composto para testar a pureza de cisatracúrio, método para testar a pureza de uma amostra de besilato de cisatracúrio, uso de um composto para testar a pureza de cisatracúrio e processo para preparar compostos para testar a pureza de cisatracúrio | |
| MX2018009339A (es) | Métodos para la identificación y análisis de secuencias de aminoácidos de las proteínas. | |
| Jia et al. | A multi-scale strategy for discovery of novel endogenous neuropeptides in the crustacean nervous system | |
| BR112016024730A2 (pt) | dispositivo, método e conjunto de análise imunocromatográfica | |
| BR112022008217A2 (pt) | Métodos e dispositivos para sequenciamento | |
| BR112014012208A2 (pt) | ensaio de fibrinogênio | |
| Ossola et al. | Biomarker validation in blood specimens by selected reaction monitoring mass spectrometry of N-glycosites | |
| BR112013027041A2 (pt) | Método de detecção de um analito e kit | |
| Arntzen et al. | Quantitative proteome analysis using isobaric peptide termini labeling (IPTL) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application fees: dismissal - article 86 of industrial property law |
Free format text: REFERENTE AS 4A E 5A ANUIDADES. |
|
| B08K | Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87) |