PE20020374A1 - Genes y enzimas de via portadora del metabolismo del carbono metanotrofico - Google Patents

Genes y enzimas de via portadora del metabolismo del carbono metanotrofico

Info

Publication number
PE20020374A1
PE20020374A1 PE2001000877A PE2001000877A PE20020374A1 PE 20020374 A1 PE20020374 A1 PE 20020374A1 PE 2001000877 A PE2001000877 A PE 2001000877A PE 2001000877 A PE2001000877 A PE 2001000877A PE 20020374 A1 PE20020374 A1 PE 20020374A1
Authority
PE
Peru
Prior art keywords
utilized
dna
ssc
refers
sds
Prior art date
Application number
PE2001000877A
Other languages
English (en)
Inventor
Mattheos Koffas
James M Odom
Kelley C Norton
Rick W Ye
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of PE20020374A1 publication Critical patent/PE20020374A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE REFIERE A ADN AISLADO QUE CODIFICA ENZIMAS COMO 2-CETO-3-DEOXI-6-FOSFOGLUCONATO, FRUCTOSA BIFOSFATO ALDOLASA EN UNA CEPA DE METHYLOMONAS SP QUE ESTA INVOLUCRADA EN LA CONVERSION DE AZUCARES HEXOSAS EN METABOLITOS DE 3 CARBONOS QUE UTILIZAN METANO COMO UNICA FUENTE DE CARBONO Y ENERGIA; SIENDO: a)ADN QUE CODIFICA SECUENCIAS DE 328, 160, 335, 358, 212, 545, 592, 437, 618, 501 AMINOACIDOS; b)ADN AISLADO QUE SE HIBRIDIZA EN CONDICIONES DE 0,1X SSC, 0,1% SDS A 65°C LAVADA CON 2X SSC, 0,1% SDS SEGUIDA POR 0,1X SSC, 0,1% SDS; c)ADN COMPLEMENTARIA; TAMBIEN SE REFIERE ADN QUE COMPRENDE UNA PRIMERA SECUENCIA NUCLEOTIDICA QUE CODIFICA UN POLIPEPTIDO DE 328 AMINOACIDOS QUE TIENE 78% DE IDENTIDAD EN BASE AL METODO DE ALINEAMIENTO DE SMITH-WATERMAN CUANDO SE COMPARA CON UN POLIPEPTIDO QUE TIENE LA SECUENCIA O EL COMPLEMENTO DE LA MISMA. LA CELULA TRANSFORMADA EN BACTERIAS METANOTROFICAS COMO ASPERGILUS, SACCHAROMYCES, PICHIA, CANDIDA, HANSENULA, SALMONELLA, BACCILLUS, ACINETOBACTER, RHODOCOCCUS, STREPTOMYCES, ESCHERICHIA, PSEUDOMONAS, METHYLOMONAS, METHYLOCOCUS, METHYLOBACTER. TAMBIEN SE REFIERE A UN METODO PARA OBTENER UN FRAGMENTO DE ADN QUE CODIFICA LA ENZIMA. O SOBREEXPRESANDO EL GEN DE FLUJO DE CARBONO. LA BIOTRANSFORMACION DEL METANO PUEDE SER POTENCIALMENTE MAS VALIOSA QUE LA ACTIVACION DE METANO A TRAVES DE MEDIOS QUIMICOS EL CUAL ES UN PROCESO TERMODINAMICAMENTE DIFICIL DE LOGRAR
PE2001000877A 2000-09-01 2001-09-03 Genes y enzimas de via portadora del metabolismo del carbono metanotrofico PE20020374A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US22990600P 2000-09-01 2000-09-01

Publications (1)

Publication Number Publication Date
PE20020374A1 true PE20020374A1 (es) 2002-06-20

Family

ID=22863154

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2001000877A PE20020374A1 (es) 2000-09-01 2001-09-03 Genes y enzimas de via portadora del metabolismo del carbono metanotrofico

Country Status (8)

Country Link
US (4) US6555353B2 (es)
EP (1) EP1313845A2 (es)
AR (1) AR033565A1 (es)
AU (1) AU2001285314A1 (es)
CA (1) CA2416936A1 (es)
NO (1) NO20030963L (es)
PE (1) PE20020374A1 (es)
WO (1) WO2002020796A2 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6689601B2 (en) * 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain
JP2004166595A (ja) 2002-11-20 2004-06-17 Ajinomoto Co Inc メチロトローフを用いたｌ−アミノ酸の製造法
GB0315783D0 (en) * 2003-07-04 2003-08-13 Norferm Da Use
US20050214838A1 (en) * 2004-03-10 2005-09-29 Waclaw Szybalski Methods for mapping and sequencing nucleic acids
BRPI0515994A (pt) * 2004-10-12 2008-08-19 Univ Michigan State biossìntese de floroglucinol e preparo de 1, 3-dihidróxibenzeno a partir do mesmo
WO2006065967A2 (en) * 2004-12-15 2006-06-22 General Atomics Allosteric enzyme coupled immunoassay (aecia)
WO2008153676A2 (en) * 2007-05-21 2008-12-18 The Regents Of The University Of California Pentose phosphate pathway and fermentation enzyme-encoding nucleotide sequences with refined translational kinetics and methods of making same
WO2012003461A2 (en) 2010-07-02 2012-01-05 Draths Corporation Phloroglucinol synthases and methods of making and using the same
WO2012006245A1 (en) 2010-07-06 2012-01-12 Draths Corporation Phloroglucinol reductase and methods of making and using the same
WO2012006244A1 (en) 2010-07-06 2012-01-12 Draths Corporation Method for producing phloroglucinol and dihydrophloroglucinol
WO2014066670A1 (en) 2012-10-24 2014-05-01 Calysta Energy, Inc. Engineering of multi-carbon substrate utilization pathways in methanotrophic bacteria
US9267158B2 (en) 2013-03-14 2016-02-23 Intrexon Corporation Biological production of multi-carbon compounds from methane
WO2015109257A1 (en) * 2014-01-16 2015-07-23 Calysta, Inc. Carbohydrate-enriched recombinant microorganisms
MY180352A (en) * 2014-04-11 2020-11-28 String Bio Private Ltd Recombinant methanotrophic bacterium and a method of production of succinic acid from methane or biogas thereof
CN109486690A (zh) * 2018-12-13 2019-03-19 江苏鸣生物科技有限公司 一种提高毕赤酵母发酵脂肪酶酶活力及甲醇蛋白产量的制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5908774A (en) 1995-04-24 1999-06-01 Shaw; Edward A. Remediation of pesticide-contaminated soil
US6689601B2 (en) * 2000-09-01 2004-02-10 E. I. Du Pont De Nemours And Company High growth methanotropic bacterial strain

Also Published As

Publication number Publication date
CA2416936A1 (en) 2002-03-14
US20040115657A1 (en) 2004-06-17
US20020110885A1 (en) 2002-08-15
NO20030963D0 (no) 2003-02-28
EP1313845A2 (en) 2003-05-28
AU2001285314A1 (en) 2002-03-22
US6555353B2 (en) 2003-04-29
US6773905B2 (en) 2004-08-10
US20030129721A1 (en) 2003-07-10
NO20030963L (no) 2003-04-28
US6908992B2 (en) 2005-06-21
AR033565A1 (es) 2003-12-26
US20030138909A1 (en) 2003-07-24
WO2002020796A2 (en) 2002-03-14
WO2002020796A3 (en) 2003-01-23
US6767744B2 (en) 2004-07-27

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