AR033565A1 - Genes y enzimas de las vias del metabolismo metanotrofico del carbono - Google Patents
Genes y enzimas de las vias del metabolismo metanotrofico del carbonoInfo
- Publication number
- AR033565A1 AR033565A1 ARP010104175A ARP010104175A AR033565A1 AR 033565 A1 AR033565 A1 AR 033565A1 AR P010104175 A ARP010104175 A AR P010104175A AR P010104175 A ARP010104175 A AR P010104175A AR 033565 A1 AR033565 A1 AR 033565A1
- Authority
- AR
- Argentina
- Prior art keywords
- genes
- nucleic acid
- carbon
- encoding
- acid molecule
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se aislaron genes de Methylomonas sp que codifican enzimas involucradas en la vía de flujo del carbono. Los genes codifican un 2-ceto-3-desoxi-6-fosfogluconato(KDPGA9) y una 2-ceto-3-deoxi-6-fosfogluconato aldolasa (FFBPA) así como numerosos otros genes. Los genes serán utiles en microorganismos que metabolizan C1 para la manipulacion de la vía de flujo del carbono. La molécula aislada de ácido nucleico que codifica una enzima del flujo de carbono de Methylomonas sp, se selecciona del grupo formado por: (a) una molécula aislada de ácido nucleico que codifica una secuencia de aminoácidos seleccionada del grupo formado por las SEQ ID N''; 2, 4, 6, 8, 10, 12, 14, 16, 18 y 20; (b) una molécula aislada de ácido nucleico que se hibridiza con (a) y se lava con SSC 0.I X. SDS 0.1%. 65s y (c) una molécula aislada de ácido nucleico que es complementaria de (a) o (b). Se proveen además los genes quiméricos que comprenden dichas moléculas de ácido nucleico y los polipéptidos codificados por dichos genes como así también células huésped transformadas que expresan dichos genes. Un método para obtener un fragmento de ácido nucleico codificador de una enzima del flujo de carbono y un método para alterar el flujo de carbono a través de una bacteria metanotrofica y un gen mutado codificador de una enzima del flujo de carbono también se describen. Genes codificadores de una vía de flujo de carbono más eficiente en cuanto a la energía permiten que una cepa bacteriana metanotrofica pueda servir como plataforma para la produccion de materiales que contengan carbono y proteínas.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22990600P | 2000-09-01 | 2000-09-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR033565A1 true AR033565A1 (es) | 2003-12-26 |
Family
ID=22863154
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP010104175A AR033565A1 (es) | 2000-09-01 | 2001-08-31 | Genes y enzimas de las vias del metabolismo metanotrofico del carbono |
Country Status (8)
Country | Link |
---|---|
US (4) | US6555353B2 (es) |
EP (1) | EP1313845A2 (es) |
AR (1) | AR033565A1 (es) |
AU (1) | AU2001285314A1 (es) |
CA (1) | CA2416936A1 (es) |
NO (1) | NO20030963L (es) |
PE (1) | PE20020374A1 (es) |
WO (1) | WO2002020796A2 (es) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6689601B2 (en) * | 2000-09-01 | 2004-02-10 | E. I. Du Pont De Nemours And Company | High growth methanotropic bacterial strain |
JP2004166595A (ja) | 2002-11-20 | 2004-06-17 | Ajinomoto Co Inc | メチロトローフを用いたl−アミノ酸の製造法 |
GB0315783D0 (en) * | 2003-07-04 | 2003-08-13 | Norferm Da | Use |
US20050214838A1 (en) * | 2004-03-10 | 2005-09-29 | Waclaw Szybalski | Methods for mapping and sequencing nucleic acids |
BRPI0515994A (pt) * | 2004-10-12 | 2008-08-19 | Univ Michigan State | biossìntese de floroglucinol e preparo de 1, 3-dihidróxibenzeno a partir do mesmo |
WO2006065967A2 (en) * | 2004-12-15 | 2006-06-22 | General Atomics | Allosteric enzyme coupled immunoassay (aecia) |
WO2008153676A2 (en) * | 2007-05-21 | 2008-12-18 | The Regents Of The University Of California | Pentose phosphate pathway and fermentation enzyme-encoding nucleotide sequences with refined translational kinetics and methods of making same |
WO2012003461A2 (en) | 2010-07-02 | 2012-01-05 | Draths Corporation | Phloroglucinol synthases and methods of making and using the same |
WO2012006245A1 (en) | 2010-07-06 | 2012-01-12 | Draths Corporation | Phloroglucinol reductase and methods of making and using the same |
WO2012006244A1 (en) | 2010-07-06 | 2012-01-12 | Draths Corporation | Method for producing phloroglucinol and dihydrophloroglucinol |
WO2014066670A1 (en) | 2012-10-24 | 2014-05-01 | Calysta Energy, Inc. | Engineering of multi-carbon substrate utilization pathways in methanotrophic bacteria |
US9267158B2 (en) | 2013-03-14 | 2016-02-23 | Intrexon Corporation | Biological production of multi-carbon compounds from methane |
WO2015109257A1 (en) * | 2014-01-16 | 2015-07-23 | Calysta, Inc. | Carbohydrate-enriched recombinant microorganisms |
MY180352A (en) * | 2014-04-11 | 2020-11-28 | String Bio Private Ltd | Recombinant methanotrophic bacterium and a method of production of succinic acid from methane or biogas thereof |
CN109486690A (zh) * | 2018-12-13 | 2019-03-19 | 江苏鸣生物科技有限公司 | 一种提高毕赤酵母发酵脂肪酶酶活力及甲醇蛋白产量的制备方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5908774A (en) | 1995-04-24 | 1999-06-01 | Shaw; Edward A. | Remediation of pesticide-contaminated soil |
US6689601B2 (en) * | 2000-09-01 | 2004-02-10 | E. I. Du Pont De Nemours And Company | High growth methanotropic bacterial strain |
-
2001
- 2001-08-22 US US09/934,901 patent/US6555353B2/en not_active Expired - Fee Related
- 2001-08-28 AU AU2001285314A patent/AU2001285314A1/en not_active Abandoned
- 2001-08-28 CA CA002416936A patent/CA2416936A1/en not_active Abandoned
- 2001-08-28 WO PCT/US2001/026730 patent/WO2002020796A2/en not_active Application Discontinuation
- 2001-08-28 EP EP01964466A patent/EP1313845A2/en not_active Withdrawn
- 2001-08-31 AR ARP010104175A patent/AR033565A1/es unknown
- 2001-09-03 PE PE2001000877A patent/PE20020374A1/es not_active Application Discontinuation
-
2002
- 2002-12-17 US US10/321,210 patent/US6767744B2/en not_active Expired - Fee Related
- 2002-12-17 US US10/320,874 patent/US6773905B2/en not_active Expired - Fee Related
- 2002-12-17 US US10/320,924 patent/US6908992B2/en not_active Expired - Fee Related
-
2003
- 2003-02-28 NO NO20030963A patent/NO20030963L/no not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CA2416936A1 (en) | 2002-03-14 |
US20040115657A1 (en) | 2004-06-17 |
US20020110885A1 (en) | 2002-08-15 |
NO20030963D0 (no) | 2003-02-28 |
EP1313845A2 (en) | 2003-05-28 |
AU2001285314A1 (en) | 2002-03-22 |
US6555353B2 (en) | 2003-04-29 |
US6773905B2 (en) | 2004-08-10 |
US20030129721A1 (en) | 2003-07-10 |
NO20030963L (no) | 2003-04-28 |
US6908992B2 (en) | 2005-06-21 |
US20030138909A1 (en) | 2003-07-24 |
WO2002020796A2 (en) | 2002-03-14 |
WO2002020796A3 (en) | 2003-01-23 |
US6767744B2 (en) | 2004-07-27 |
PE20020374A1 (es) | 2002-06-20 |
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