OA11419A - HIV-1tat, or derivatives thereof for prophylactic and therapeutic vaccination. - Google Patents
HIV-1tat, or derivatives thereof for prophylactic and therapeutic vaccination. Download PDFInfo
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- OA11419A OA11419A OA1200000159A OA1200000159A OA11419A OA 11419 A OA11419 A OA 11419A OA 1200000159 A OA1200000159 A OA 1200000159A OA 1200000159 A OA1200000159 A OA 1200000159A OA 11419 A OA11419 A OA 11419A
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- tat
- dna
- aids
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- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16041—Use of virus, viral particle or viral elements as a vector
- C12N2710/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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IT97RM000743A IT1297090B1 (it) | 1997-12-01 | 1997-12-01 | Tat di hiv-1 o suoi derivati, da soli od in combinazione, a scopo vaccinale, profilattico e terapeutico, contro l'aids i tumori e le |
Publications (1)
Publication Number | Publication Date |
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OA11419A true OA11419A (en) | 2004-04-21 |
Family
ID=11405378
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
OA1200000159A OA11419A (en) | 1997-12-01 | 2000-05-30 | HIV-1tat, or derivatives thereof for prophylactic and therapeutic vaccination. |
Country Status (23)
Country | Link |
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US (2) | US7744896B1 (el) |
EP (2) | EP1698347A3 (el) |
JP (1) | JP2001524531A (el) |
CN (1) | CN1283121A (el) |
AP (1) | AP2000001828A0 (el) |
AT (1) | ATE374041T1 (el) |
AU (1) | AU762893B2 (el) |
BR (1) | BR9814725A (el) |
CA (1) | CA2311647C (el) |
CU (1) | CU20000126A7 (el) |
CY (1) | CY1107818T1 (el) |
DE (1) | DE69838486T2 (el) |
DK (1) | DK1035865T3 (el) |
EA (1) | EA004330B1 (el) |
ES (1) | ES2293700T3 (el) |
IL (1) | IL136372A0 (el) |
IT (1) | IT1297090B1 (el) |
NZ (1) | NZ505373A (el) |
OA (1) | OA11419A (el) |
PL (1) | PL341818A1 (el) |
PT (1) | PT1035865E (el) |
TR (1) | TR200001553T2 (el) |
WO (1) | WO1999027958A2 (el) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150359878A1 (en) * | 2013-01-22 | 2015-12-17 | Vaxxit Srl | Use of replication deficient hsv-1 as a vaccine vector for the deli vary of hiv-1 tat antigen |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1297090B1 (it) | 1997-12-01 | 1999-08-03 | Barbara Ensoli | Tat di hiv-1 o suoi derivati, da soli od in combinazione, a scopo vaccinale, profilattico e terapeutico, contro l'aids i tumori e le |
US20030194800A1 (en) * | 2001-08-31 | 2003-10-16 | Megede Jan Zur | Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides and uses thereof |
US20010008627A1 (en) * | 1999-01-22 | 2001-07-19 | Soll David R. | Hiv-encoded chemoattractant |
CA2367692A1 (en) * | 1999-03-15 | 2000-09-21 | Introgen Therapeutics, Inc. | Dendritic cells transduced with a wild-type self gene elicit potent antitumor immune responses |
AU4082300A (en) * | 1999-06-21 | 2001-01-09 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Hiv tat peptides and multiple peptide conjugate system |
KR100379577B1 (ko) * | 2000-07-26 | 2003-04-08 | 최수영 | 세포침투성 티에이티 - 인간 글루탐산 탈수소효소 융합단백질, 이 융합단백질을 코딩하는 재조합폴리뉴클레오타이드, 이 융합단백질의 발현벡터 및 이융합단백질을 세포 내로 도입하는 방법 |
NO314587B1 (no) * | 2000-09-04 | 2003-04-14 | Bionor Immuno As | HIV regulatoriske- og hjelpepeptider, antigener, vaksinepreparater, immunoassay testsett og en metode for påvisning av antistoffer fremkaltav HIV |
EP1279404A1 (en) * | 2001-07-26 | 2003-01-29 | Istituto Superiore di Sanità | Use of HIV-1 tat, fragments or derivatives thereof, to target or to activate antigen-presenting cells, to deliver cargo molecules for vaccination or to treat other diseases |
AU2002358181A1 (en) * | 2001-12-11 | 2003-07-09 | Aventis Pasteur | Mutated hiv tat |
FR2834717B1 (fr) * | 2002-01-11 | 2004-12-10 | Bio Merieux | Mutants de la proteine tat du virus vih-1 |
UA85373C2 (ru) | 2002-05-16 | 2009-01-26 | Бавариан Нордик А/С | Слитый протеин регуляторных/аксессорных hіv протеинов |
AU2003268688A1 (en) * | 2002-09-30 | 2004-04-19 | Yamanouchi Pharmaceutical Co., Ltd. | Method of inducing immune responses |
CA2506695A1 (en) * | 2002-11-19 | 2004-06-03 | The Trustees Of The University Of Pennsylvania | Genetic constructs and compositions comprising rre and cte and uses thereof |
AU2003292136A1 (en) * | 2002-11-26 | 2004-06-18 | Eurocine Ab | Vaccines against viruses with cationic substances as adjuvants |
CA2510309C (en) | 2002-12-16 | 2014-09-16 | Liyange P. Perera | Recombinant vaccine viruses expressing il-15 and methods of using the same |
GB0323840D0 (en) * | 2003-10-10 | 2003-11-12 | Ist Superiore Sanita | Vaccines |
US20090123468A1 (en) | 2003-10-24 | 2009-05-14 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
US8507277B2 (en) | 2003-10-24 | 2013-08-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides |
US8133733B2 (en) | 2003-10-24 | 2012-03-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to target tissues |
US8062891B2 (en) | 2003-10-24 | 2011-11-22 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to plants |
ES2411962T3 (es) | 2003-10-24 | 2013-07-09 | Gencia Corporation | Métodos y composiciones para suministrar polinucleótidos |
GB2407501A (en) * | 2003-11-03 | 2005-05-04 | Ist Superiore Sanita | Nanoparticles for delivery of a pharmacologically active agent, comprising water insoluble (co)polymer core & hydrophilic acrylate-based (co)polymer shell |
US20050208482A1 (en) | 2004-03-16 | 2005-09-22 | Cohen David I | Tat-based immunomodulatory compositions and methods for their discovery and use |
FR2868318B1 (fr) * | 2004-04-01 | 2012-11-16 | Commissariat Energie Atomique | Antigene tat stabilise et ses applications pour la vaccination anti-vih |
TW200613554A (en) * | 2004-06-17 | 2006-05-01 | Wyeth Corp | Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV |
AU2006249199A1 (en) | 2005-05-12 | 2006-11-23 | Introgen Therapeutics, Inc. | P53 vaccines for the treatment of cancers |
US7943140B2 (en) | 2006-08-14 | 2011-05-17 | Thymon, Llc | Compositions and methods for the treatment and prophylaxis of multiple strains and subtypes of HIV-1 |
GB0802224D0 (en) * | 2008-02-06 | 2008-03-12 | Inst Superiore Di Sanito | Process for the production of vaccine components |
WO2009139004A2 (en) * | 2008-05-14 | 2009-11-19 | Jawaharlal Nehru Centre For Advanced Scientific Research | Tat dna sequences, gene constructs, vaccine and processes thereof |
BRPI1009215B1 (pt) | 2009-03-23 | 2019-10-29 | Nanirx Inc | composição farmacêutica compreendendo uma sequência de aminoácidos modificada da proteína trans-ativadora de transcrição (tat) do vírus da imunodeficiência humana (hiv) e uso de um polipeptídeo derivado de tat para tratar câncer |
CN102153657A (zh) * | 2011-01-18 | 2011-08-17 | 陕西师范大学 | IL-24-Tat PTD融合蛋白及其构建方法和应用 |
WO2014151771A1 (en) * | 2013-03-14 | 2014-09-25 | The J. David Gladstone Institutes | Compositions and methods for treating an immunodeficiency virus infection |
US9663556B2 (en) | 2013-10-04 | 2017-05-30 | Pin Pharma, Inc. | Treatment of cancers with immunostimulatory HIV tat derivative polypeptides |
CN103936863B (zh) * | 2014-04-23 | 2017-01-04 | 西安交通大学 | 一种调控stat3磷酸化的融合肽及其应用 |
CN104001155B (zh) * | 2014-06-12 | 2016-04-13 | 中山大学 | 一种Tat蛋白及其制备方法和应用 |
EP2982693A1 (en) * | 2014-08-07 | 2016-02-10 | Affimed Therapeutics AG | CD3 binding domain |
WO2018217897A1 (en) * | 2017-05-23 | 2018-11-29 | David Weiner | Compositions and method for inducing an immune response |
CN109943590B (zh) * | 2019-04-18 | 2022-11-18 | 西南大学 | 一种复制缺陷型猪圆环病毒、其制备方法及应用 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PT83682B (en) * | 1985-11-06 | 1988-12-05 | Smithkline Beckman Corp | Process for the expression of an aids virus gene |
US5674980A (en) * | 1989-12-21 | 1997-10-07 | Biogen Inc | Fusion protein comprising tat-derived transport moiety |
US5646120A (en) * | 1990-10-24 | 1997-07-08 | Allelix Biopharmaceuticals, Inc. | Peptide-based inhibitors of HIV replication |
EP0491218A1 (en) * | 1990-12-17 | 1992-06-24 | F. Hoffmann-La Roche Ag | Benzodiazepinones |
US6107062A (en) | 1992-07-30 | 2000-08-22 | Inpax, Inc. | Antisense viruses and antisense-ribozyme viruses |
WO1994015634A1 (en) * | 1992-12-30 | 1994-07-21 | Matthias Rath | Tat and rev oligopeptides in hiv treatment |
EP0767678A1 (en) | 1994-05-23 | 1997-04-16 | Gideon Goldstein | Compositions of transactivating proteins of human immunodeficiency virus |
US6024965A (en) | 1996-10-18 | 2000-02-15 | Erasums University Rotterdam | Induction of REV and TAT specific cytotoxic T-cells for prevention and treatment of human immunodeficiency virus (HIV) infection |
IT1297090B1 (it) | 1997-12-01 | 1999-08-03 | Barbara Ensoli | Tat di hiv-1 o suoi derivati, da soli od in combinazione, a scopo vaccinale, profilattico e terapeutico, contro l'aids i tumori e le |
OA12168A (en) * | 2000-01-31 | 2006-05-08 | Smithkline Beecham Biolog | Vaccine for the prophylactic or therapeutic immunization against HIV. |
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1997
- 1997-12-01 IT IT97RM000743A patent/IT1297090B1/it active IP Right Grant
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1998
- 1998-11-30 EA EA200000596A patent/EA004330B1/ru not_active IP Right Cessation
- 1998-11-30 IL IL13637298A patent/IL136372A0/xx not_active IP Right Cessation
- 1998-11-30 PL PL98341818A patent/PL341818A1/xx unknown
- 1998-11-30 AT AT98966601T patent/ATE374041T1/de active
- 1998-11-30 BR BR9814725-0A patent/BR9814725A/pt not_active IP Right Cessation
- 1998-11-30 AP APAP/P/2000/001828A patent/AP2000001828A0/en unknown
- 1998-11-30 AU AU24126/99A patent/AU762893B2/en not_active Ceased
- 1998-11-30 PT PT98966601T patent/PT1035865E/pt unknown
- 1998-11-30 CA CA2311647A patent/CA2311647C/en not_active Expired - Fee Related
- 1998-11-30 US US09/555,534 patent/US7744896B1/en not_active Expired - Fee Related
- 1998-11-30 CN CN98812496A patent/CN1283121A/zh active Pending
- 1998-11-30 JP JP2000522943A patent/JP2001524531A/ja active Pending
- 1998-11-30 NZ NZ505373A patent/NZ505373A/en not_active IP Right Cessation
- 1998-11-30 DE DE69838486T patent/DE69838486T2/de not_active Expired - Lifetime
- 1998-11-30 EP EP05026908A patent/EP1698347A3/en not_active Withdrawn
- 1998-11-30 ES ES98966601T patent/ES2293700T3/es not_active Expired - Lifetime
- 1998-11-30 EP EP98966601A patent/EP1035865B1/en not_active Expired - Lifetime
- 1998-11-30 DK DK98966601T patent/DK1035865T3/da active
- 1998-11-30 WO PCT/EP1998/007721 patent/WO1999027958A2/en active IP Right Grant
- 1998-11-30 TR TR2000/01553T patent/TR200001553T2/xx unknown
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2000
- 2000-05-30 OA OA1200000159A patent/OA11419A/en unknown
- 2000-05-31 CU CU20000126A patent/CU20000126A7/es unknown
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2007
- 2007-11-29 CY CY20071101530T patent/CY1107818T1/el unknown
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2009
- 2009-05-06 US US12/436,119 patent/US8197820B2/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150359878A1 (en) * | 2013-01-22 | 2015-12-17 | Vaxxit Srl | Use of replication deficient hsv-1 as a vaccine vector for the deli vary of hiv-1 tat antigen |
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