NZ516894A - Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis - Google Patents
Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesisInfo
- Publication number
- NZ516894A NZ516894A NZ516894A NZ51689400A NZ516894A NZ 516894 A NZ516894 A NZ 516894A NZ 516894 A NZ516894 A NZ 516894A NZ 51689400 A NZ51689400 A NZ 51689400A NZ 516894 A NZ516894 A NZ 516894A
- Authority
- NZ
- New Zealand
- Prior art keywords
- nucleic acid
- oligonucleotide
- polymerase
- inhibitor
- synthesis
- Prior art date
Links
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/09—Recombinant DNA-technology
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- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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| US14207299P | 1999-07-02 | 1999-07-02 | |
| PCT/US2000/018256 WO2001002559A1 (en) | 1999-07-02 | 2000-06-30 | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
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| NZ516894A NZ516894A (en) | 1999-07-02 | 2000-06-30 | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
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| CN100351393C (zh) | 2001-02-15 | 2007-11-28 | 宝生物工程株式会社 | 核苷酸多态性的检测方法 |
| WO2004024919A1 (en) * | 2002-09-13 | 2004-03-25 | Replicor, Inc. | Non-sequence complementary antiviral oligonucleotides |
| CN101076608B (zh) * | 2004-10-18 | 2013-04-17 | 布兰迪斯大学 | 在pcr扩增中提高再现性和降低引物错导的试剂和方法 |
| US8530194B2 (en) | 2005-09-26 | 2013-09-10 | Allelogic Biosciences Corporation | Oligonucleotides as temperature-sensitive inhibitors for DNA polymerases |
| EP2458011B1 (en) * | 2005-10-03 | 2013-07-24 | Life Technologies Corporation | Compositions, methods, and kits for amplifying nucleic acids |
| US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
| SG190377A1 (en) | 2010-11-24 | 2013-06-28 | Kaneka Corp | Amplified nucleic acid detection method and detection device |
| JP6490576B2 (ja) * | 2012-04-12 | 2019-03-27 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | 重合反応のための合成核酸 |
| WO2013162026A1 (ja) * | 2012-04-27 | 2013-10-31 | 株式会社カネカ | 核酸の増幅方法、および、増幅核酸の検出方法 |
| US10392652B2 (en) | 2013-11-22 | 2019-08-27 | Kaneka Corporation | Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end |
| US11970731B2 (en) | 2018-01-05 | 2024-04-30 | Roche Sequencing Solutions, Inc. | Enhancement of nucleic acid polymerization by aromatic compounds |
| KR102275038B1 (ko) | 2019-12-11 | 2021-07-08 | (주) 제노텍 | 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트 |
| CN115135664A (zh) * | 2020-06-22 | 2022-09-30 | 苏州新海生物科技股份有限公司 | 一种核酸配体及其应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5763173A (en) * | 1990-06-11 | 1998-06-09 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| DE69638318D1 (de) * | 1995-06-07 | 2011-02-17 | Gilead Sciences Inc | Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren |
| US5760012A (en) * | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
-
2000
- 2000-06-30 JP JP2001508332A patent/JP2003504018A/ja active Pending
- 2000-06-30 DE DE60029225T patent/DE60029225T2/de not_active Expired - Lifetime
- 2000-06-30 WO PCT/US2000/018256 patent/WO2001002559A1/en not_active Ceased
- 2000-06-30 EP EP00943373A patent/EP1196559B1/en not_active Expired - Lifetime
- 2000-06-30 AU AU57850/00A patent/AU782555B2/en not_active Ceased
- 2000-06-30 AT AT00943373T patent/ATE332371T1/de not_active IP Right Cessation
- 2000-06-30 CA CA002377780A patent/CA2377780A1/en not_active Abandoned
- 2000-06-30 NZ NZ516894A patent/NZ516894A/en unknown
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2010
- 2010-10-15 JP JP2010232252A patent/JP2011041572A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ATE332371T1 (de) | 2006-07-15 |
| JP2003504018A (ja) | 2003-02-04 |
| JP2011041572A (ja) | 2011-03-03 |
| EP1196559B1 (en) | 2006-07-05 |
| WO2001002559A9 (en) | 2002-07-25 |
| DE60029225T2 (de) | 2007-07-05 |
| AU5785000A (en) | 2001-01-22 |
| DE60029225D1 (de) | 2006-08-17 |
| CA2377780A1 (en) | 2001-01-11 |
| AU782555B2 (en) | 2005-08-11 |
| EP1196559A1 (en) | 2002-04-17 |
| WO2001002559A1 (en) | 2001-01-11 |
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