NO844848L

NO844848L - Hybridiseringsanalyse med immobilisering av hybrider ved anti-hybrid binding

Hybridiseringsanalyse med immobilisering av hybrider ved anti-hybrid binding

Info

Publication number: NO844848L
Authority: NO; Norway
Prior art keywords: dna; rna; probe; antibody; immobilized
Prior art date: 1983-12-12

Application number

NO844848A

Other languages

English (en)

Norwegian (no)

Inventor

James P Albarella

Leslie H Deriemer Anderson

Robert J Carrico

Original Assignee

Miles Lab

Priority date

1983-12-12

Filing date

1984-12-04

Publication date

1985-06-13

1984-12-04 Application filed by Miles Lab filed Critical Miles Lab

1985-06-13 Publication of NO844848L publication Critical patent/NO844848L/no

Links

238000009396 hybridization Methods 0.000 title claims description 62
238000009739 binding Methods 0.000 title claims description 45
230000027455 binding Effects 0.000 title claims description 44
238000004458 analytical method Methods 0.000 title claims description 23
239000000523 sample Substances 0.000 claims description 132
238000000034 method Methods 0.000 claims description 83
239000003153 chemical reaction reagent Substances 0.000 claims description 75
102000039446 nucleic acids Human genes 0.000 claims description 67
108020004707 nucleic acids Proteins 0.000 claims description 67
150000007523 nucleic acids Chemical class 0.000 claims description 67
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 34
239000000126 substance Substances 0.000 claims description 24
230000000295 complement effect Effects 0.000 claims description 18
102000040430 polynucleotide Human genes 0.000 claims description 18
108091033319 polynucleotide Proteins 0.000 claims description 18
239000002157 polynucleotide Substances 0.000 claims description 18
235000020958 biotin Nutrition 0.000 claims description 17
239000011616 biotin Substances 0.000 claims description 17
229960002685 biotin Drugs 0.000 claims description 17
239000003446 ligand Substances 0.000 claims description 15
238000009830 intercalation Methods 0.000 claims description 9
230000002687 intercalation Effects 0.000 claims description 9
108090001008 Avidin Proteins 0.000 claims description 8
108020004711 Nucleic Acid Probes Proteins 0.000 claims description 4
239000002853 nucleic acid probe Substances 0.000 claims description 4
239000012472 biological sample Substances 0.000 claims 1
230000002349 favourable effect Effects 0.000 claims 1
239000000138 intercalating agent Substances 0.000 claims 1
108091032973 (ribonucleotides)n+m Proteins 0.000 description 61
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 36
239000000243 solution Substances 0.000 description 27
239000000872 buffer Substances 0.000 description 25
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 24
108090000623 proteins and genes Proteins 0.000 description 22
108020004518 RNA Probes Proteins 0.000 description 20
239000003391 RNA probe Substances 0.000 description 20
102000004169 proteins and genes Human genes 0.000 description 20
102000004190 Enzymes Human genes 0.000 description 19
108090000790 Enzymes Proteins 0.000 description 19
229940088598 enzyme Drugs 0.000 description 19
239000000203 mixture Substances 0.000 description 19
238000003556 assay Methods 0.000 description 18
238000006243 chemical reaction Methods 0.000 description 18
239000011780 sodium chloride Substances 0.000 description 18
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 16
239000002585 base Substances 0.000 description 15
238000002372 labelling Methods 0.000 description 15
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 14
QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 14
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 14
239000011541 reaction mixture Substances 0.000 description 14
108020004414 DNA Proteins 0.000 description 11
102000053602 DNA Human genes 0.000 description 11
210000004027 cell Anatomy 0.000 description 11
239000007790 solid phase Substances 0.000 description 11
241000701022 Cytomegalovirus Species 0.000 description 10
101150023114 RNA1 gene Proteins 0.000 description 10
239000002253 acid Substances 0.000 description 10
239000012634 fragment Substances 0.000 description 10
238000002360 preparation method Methods 0.000 description 10
239000007787 solid Substances 0.000 description 10
210000002700 urine Anatomy 0.000 description 10
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
238000001514 detection method Methods 0.000 description 9
108060003951 Immunoglobulin Proteins 0.000 description 8
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 8
RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 8
230000003053 immunization Effects 0.000 description 8
238000002649 immunization Methods 0.000 description 8
230000002163 immunogen Effects 0.000 description 8
102000018358 immunoglobulin Human genes 0.000 description 8
239000002244 precipitate Substances 0.000 description 8
239000000047 product Substances 0.000 description 8
239000013598 vector Substances 0.000 description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 7
IMFJTOBLMOJLDM-UHFFFAOYSA-O 8-azido-5-ethyl-6-phenylphenanthridin-5-ium-3-amine Chemical compound C12=CC(N=[N+]=[N-])=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 IMFJTOBLMOJLDM-UHFFFAOYSA-O 0.000 description 7
239000003298 DNA probe Substances 0.000 description 7
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 7
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 7
239000007995 HEPES buffer Substances 0.000 description 7
238000007899 nucleic acid hybridization Methods 0.000 description 7
239000002773 nucleotide Substances 0.000 description 7
125000003729 nucleotide group Chemical group 0.000 description 7
239000012071 phase Substances 0.000 description 7
-1 phosphorus thiolates Chemical class 0.000 description 7
238000000926 separation method Methods 0.000 description 7
239000012064 sodium phosphate buffer Substances 0.000 description 7
239000000758 substrate Substances 0.000 description 7
230000008878 coupling Effects 0.000 description 6
238000010168 coupling process Methods 0.000 description 6
238000005859 coupling reaction Methods 0.000 description 6
210000004408 hybridoma Anatomy 0.000 description 6
239000000463 material Substances 0.000 description 6
238000001179 sorption measurement Methods 0.000 description 6
239000000725 suspension Substances 0.000 description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
241000588724 Escherichia coli Species 0.000 description 5
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 5
108020004682 Single-Stranded DNA Proteins 0.000 description 5
230000015572 biosynthetic process Effects 0.000 description 5
238000005119 centrifugation Methods 0.000 description 5
238000000605 extraction Methods 0.000 description 5
229940072221 immunoglobulins Drugs 0.000 description 5
239000002609 medium Substances 0.000 description 5
238000012986 modification Methods 0.000 description 5
230000004048 modification Effects 0.000 description 5
238000006303 photolysis reaction Methods 0.000 description 5
239000013612 plasmid Substances 0.000 description 5
241000894007 species Species 0.000 description 5
108091003079 Bovine Serum Albumin Proteins 0.000 description 4
102000004594 DNA Polymerase I Human genes 0.000 description 4
108010017826 DNA Polymerase I Proteins 0.000 description 4
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 4
108010024636 Glutathione Proteins 0.000 description 4
241000699670 Mus sp. Species 0.000 description 4
241000276498 Pollachius virens Species 0.000 description 4
108010090804 Streptavidin Proteins 0.000 description 4
108010034949 Thyroglobulin Proteins 0.000 description 4
102000009843 Thyroglobulin Human genes 0.000 description 4
241000700605 Viruses Species 0.000 description 4
238000002835 absorbance Methods 0.000 description 4
150000007513 acids Chemical class 0.000 description 4
230000003172 anti-dna Effects 0.000 description 4
229940098773 bovine serum albumin Drugs 0.000 description 4
230000001419 dependent effect Effects 0.000 description 4
230000000694 effects Effects 0.000 description 4
229960005542 ethidium bromide Drugs 0.000 description 4
229960003180 glutathione Drugs 0.000 description 4
239000007788 liquid Substances 0.000 description 4
229910001629 magnesium chloride Inorganic materials 0.000 description 4
230000015843 photosynthesis, light reaction Effects 0.000 description 4
108091008146 restriction endonucleases Proteins 0.000 description 4
229960002175 thyroglobulin Drugs 0.000 description 4
WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
108010088751 Albumins Proteins 0.000 description 3
102000009027 Albumins Human genes 0.000 description 3
102000002260 Alkaline Phosphatase Human genes 0.000 description 3
108020004774 Alkaline Phosphatase Proteins 0.000 description 3
241001465754 Metazoa Species 0.000 description 3
ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
229920001213 Polysorbate 20 Polymers 0.000 description 3
108091028664 Ribonucleotide Proteins 0.000 description 3
239000003513 alkali Substances 0.000 description 3
210000004369 blood Anatomy 0.000 description 3
239000008280 blood Substances 0.000 description 3
125000003636 chemical group Chemical group 0.000 description 3
239000003795 chemical substances by application Substances 0.000 description 3
238000007796 conventional method Methods 0.000 description 3
238000013461 design Methods 0.000 description 3
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 3
230000009977 dual effect Effects 0.000 description 3
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 3
239000000499 gel Substances 0.000 description 3
239000011521 glass Substances 0.000 description 3
229920001519 homopolymer Polymers 0.000 description 3
238000011065 in-situ storage Methods 0.000 description 3
238000010348 incorporation Methods 0.000 description 3
238000011534 incubation Methods 0.000 description 3
239000003112 inhibitor Substances 0.000 description 3
238000004519 manufacturing process Methods 0.000 description 3
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 3
230000000704 physical effect Effects 0.000 description 3
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
239000002336 ribonucleotide Substances 0.000 description 3
210000002966 serum Anatomy 0.000 description 3
230000009870 specific binding Effects 0.000 description 3
238000012360 testing method Methods 0.000 description 3
238000013518 transcription Methods 0.000 description 3
230000035897 transcription Effects 0.000 description 3
210000002845 virion Anatomy 0.000 description 3
KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
229920000936 Agarose Polymers 0.000 description 2
206010003445 Ascites Diseases 0.000 description 2
241000972773 Aulopiformes Species 0.000 description 2
238000011725 BALB/c mouse Methods 0.000 description 2
108020003215 DNA Probes Proteins 0.000 description 2
230000006820 DNA synthesis Effects 0.000 description 2
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
108010058683 Immobilized Proteins Proteins 0.000 description 2
UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 2
241000699666 Mus <mouse, genus> Species 0.000 description 2
239000000020 Nitrocellulose Substances 0.000 description 2
102000003992 Peroxidases Human genes 0.000 description 2
206010035226 Plasma cell myeloma Diseases 0.000 description 2
108020004511 Recombinant DNA Proteins 0.000 description 2
102000006382 Ribonucleases Human genes 0.000 description 2
108010083644 Ribonucleases Proteins 0.000 description 2
241001468001 Salmonella virus SP6 Species 0.000 description 2
229920005654 Sephadex Polymers 0.000 description 2
239000012507 Sephadex™ Substances 0.000 description 2
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
239000002671 adjuvant Substances 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
239000000427 antigen Substances 0.000 description 2
108091007433 antigens Proteins 0.000 description 2
102000036639 antigens Human genes 0.000 description 2
239000011324 bead Substances 0.000 description 2
230000008901 benefit Effects 0.000 description 2
150000001720 carbohydrates Chemical class 0.000 description 2
235000014633 carbohydrates Nutrition 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
230000015556 catabolic process Effects 0.000 description 2
238000007385 chemical modification Methods 0.000 description 2
238000004587 chromatography analysis Methods 0.000 description 2
239000005515 coenzyme Substances 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
230000000875 corresponding effect Effects 0.000 description 2
ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
238000006731 degradation reaction Methods 0.000 description 2
238000004925 denaturation Methods 0.000 description 2
230000036425 denaturation Effects 0.000 description 2
150000002118 epoxides Chemical class 0.000 description 2
230000005284 excitation Effects 0.000 description 2
239000012530 fluid Substances 0.000 description 2
UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
229910052739 hydrogen Inorganic materials 0.000 description 2
239000001257 hydrogen Substances 0.000 description 2
238000003018 immunoassay Methods 0.000 description 2
239000011859 microparticle Substances 0.000 description 2
201000000050 myeloid neoplasm Diseases 0.000 description 2
229920001220 nitrocellulos Polymers 0.000 description 2
239000002245 particle Substances 0.000 description 2
108040007629 peroxidase activity proteins Proteins 0.000 description 2
230000000644 propagated effect Effects 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
230000035484 reaction time Effects 0.000 description 2
238000011160 research Methods 0.000 description 2
239000011347 resin Substances 0.000 description 2
229920005989 resin Polymers 0.000 description 2
230000004044 response Effects 0.000 description 2
125000002652 ribonucleotide group Chemical group 0.000 description 2
235000019515 salmon Nutrition 0.000 description 2
229920006395 saturated elastomer Polymers 0.000 description 2
239000001509 sodium citrate Substances 0.000 description 2
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 2
210000004989 spleen cell Anatomy 0.000 description 2
239000007858 starting material Substances 0.000 description 2
230000000638 stimulation Effects 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
238000003786 synthesis reaction Methods 0.000 description 2
238000013519 translation Methods 0.000 description 2
239000001226 triphosphate Substances 0.000 description 2
235000011178 triphosphate Nutrition 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
150000003923 2,5-pyrrolediones Chemical class 0.000 description 1
NHXWOZNNPSXUKN-UHFFFAOYSA-N 2-azido-9h-fluorene Chemical compound C1=CC=C2C3=CC=C(N=[N+]=[N-])C=C3CC2=C1 NHXWOZNNPSXUKN-UHFFFAOYSA-N 0.000 description 1
GZFVOFMKXXTWQE-UHFFFAOYSA-N 3,8-diazido-5-ethyl-6-phenylphenanthridin-5-ium Chemical class C12=CC(N=[N+]=[N-])=CC=C2C2=CC=C(N=[N+]=[N-])C=C2[N+](CC)=C1C1=CC=CC=C1 GZFVOFMKXXTWQE-UHFFFAOYSA-N 0.000 description 1
FAPUKIBZMFELIY-UHFFFAOYSA-N 3-azido-5-ethyl-6-phenylphenanthridin-5-ium-8-amine Chemical class C12=CC(N)=CC=C2C2=CC=C(N=[N+]=[N-])C=C2[N+](CC)=C1C1=CC=CC=C1 FAPUKIBZMFELIY-UHFFFAOYSA-N 0.000 description 1
MDQUSQOIBBZWLQ-UHFFFAOYSA-N 3-azidoacridine Chemical compound C1=CC=CC2=NC3=CC(N=[N+]=[N-])=CC=C3C=C21 MDQUSQOIBBZWLQ-UHFFFAOYSA-N 0.000 description 1
YFHYLFGKDYAHBW-UHFFFAOYSA-N 4-azido-7-chloroquinoline Chemical compound [N-]=[N+]=NC1=CC=NC2=CC(Cl)=CC=C21 YFHYLFGKDYAHBW-UHFFFAOYSA-N 0.000 description 1
ZLOIGESWDJYCTF-XVFCMESISA-N 4-thiouridine Chemical group O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=S)C=C1 ZLOIGESWDJYCTF-XVFCMESISA-N 0.000 description 1
GHUXAYLZEGLXDA-UHFFFAOYSA-N 8-azido-5-ethyl-6-phenylphenanthridin-5-ium-3-amine;bromide Chemical compound [Br-].C12=CC(N=[N+]=[N-])=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 GHUXAYLZEGLXDA-UHFFFAOYSA-N 0.000 description 1
BFOMVRGJRFLFCJ-UHFFFAOYSA-N 9-azidoacridine Chemical compound C1=CC=C2C(N=[N+]=[N-])=C(C=CC=C3)C3=NC2=C1 BFOMVRGJRFLFCJ-UHFFFAOYSA-N 0.000 description 1
108091092742 A-DNA Proteins 0.000 description 1
229930195730 Aflatoxin Natural products 0.000 description 1
IVRMZWNICZWHMI-UHFFFAOYSA-N Azide Chemical class [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
101000800130 Bos taurus Thyroglobulin Proteins 0.000 description 1
241000283707 Capra Species 0.000 description 1
239000004215 Carbon black (E152) Substances 0.000 description 1
102000014914 Carrier Proteins Human genes 0.000 description 1
108010078791 Carrier Proteins Proteins 0.000 description 1
241000700199 Cavia porcellus Species 0.000 description 1
102000018832 Cytochromes Human genes 0.000 description 1
108010052832 Cytochromes Proteins 0.000 description 1
229920002271 DEAE-Sepharose Polymers 0.000 description 1
108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 1
102000016911 Deoxyribonucleases Human genes 0.000 description 1
108010053770 Deoxyribonucleases Proteins 0.000 description 1
AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
108010067770 Endopeptidase K Proteins 0.000 description 1
241001302129 Fiji disease virus Species 0.000 description 1
239000004366 Glucose oxidase Substances 0.000 description 1
108010015776 Glucose oxidase Proteins 0.000 description 1
241000644555 Guppy reovirus Species 0.000 description 1
102000006947 Histones Human genes 0.000 description 1
108010033040 Histones Proteins 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
208000026350 Inborn Genetic disease Diseases 0.000 description 1
108090001090 Lectins Proteins 0.000 description 1
102000004856 Lectins Human genes 0.000 description 1
241001082241 Lythrum hyssopifolia Species 0.000 description 1
229930192392 Mitomycin Natural products 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
108090000284 Pepsin A Proteins 0.000 description 1
102000057297 Pepsin A Human genes 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
108010065868 RNA polymerase SP6 Proteins 0.000 description 1
239000013614 RNA sample Substances 0.000 description 1
JQYMGXZJTCOARG-UHFFFAOYSA-N Reactive blue 2 Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC(S(O)(=O)=O)=C1 JQYMGXZJTCOARG-UHFFFAOYSA-N 0.000 description 1
241000405383 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Species 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
206010043395 Thalassaemia sickle cell Diseases 0.000 description 1
102000004357 Transferases Human genes 0.000 description 1
108090000992 Transferases Proteins 0.000 description 1
108090000901 Transferrin Proteins 0.000 description 1
102000004338 Transferrin Human genes 0.000 description 1
108020005202 Viral DNA Proteins 0.000 description 1
238000005299 abrasion Methods 0.000 description 1
238000011481 absorbance measurement Methods 0.000 description 1
239000000999 acridine dye Substances 0.000 description 1
DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
150000001253 acrylic acids Chemical class 0.000 description 1
239000003463 adsorbent Substances 0.000 description 1
239000005409 aflatoxin Substances 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
PPQRONHOSHZGFQ-LMVFSUKVSA-N aldehydo-D-ribose 5-phosphate Chemical group OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PPQRONHOSHZGFQ-LMVFSUKVSA-N 0.000 description 1
239000002168 alkylating agent Substances 0.000 description 1
229940100198 alkylating agent Drugs 0.000 description 1
239000012805 animal sample Substances 0.000 description 1
125000000129 anionic group Chemical group 0.000 description 1
229940111121 antirheumatic drug quinolines Drugs 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
239000003125 aqueous solvent Substances 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
150000001541 aziridines Chemical class 0.000 description 1
230000004888 barrier function Effects 0.000 description 1
DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
102000005936 beta-Galactosidase Human genes 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
230000008275 binding mechanism Effects 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
210000001124 body fluid Anatomy 0.000 description 1
239000010839 body fluid Substances 0.000 description 1
238000009835 boiling Methods 0.000 description 1
229910021538 borax Inorganic materials 0.000 description 1
244000309464 bull Species 0.000 description 1
229910052792 caesium Inorganic materials 0.000 description 1
TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
244000309466 calf Species 0.000 description 1
201000011510 cancer Diseases 0.000 description 1
125000002091 cationic group Chemical group 0.000 description 1
108091092356 cellular DNA Proteins 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
210000001175 cerebrospinal fluid Anatomy 0.000 description 1
238000002144 chemical decomposition reaction Methods 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
239000012459 cleaning agent Substances 0.000 description 1
230000021615 conjugation Effects 0.000 description 1
230000002596 correlated effect Effects 0.000 description 1
229960000956 coumarin Drugs 0.000 description 1
235000001671 coumarin Nutrition 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
238000006352 cycloaddition reaction Methods 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
238000000432 density-gradient centrifugation Methods 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
MXHRCPNRJAMMIM-UHFFFAOYSA-N desoxyuridine Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-UHFFFAOYSA-N 0.000 description 1
239000003599 detergent Substances 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
238000009792 diffusion process Methods 0.000 description 1
230000029087 digestion Effects 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
MWEQTWJABOLLOS-UHFFFAOYSA-L disodium;[[[5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-oxidophosphoryl] hydrogen phosphate;trihydrate Chemical compound O.O.O.[Na+].[Na+].C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)C(O)C1O MWEQTWJABOLLOS-UHFFFAOYSA-L 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
239000003480 eluent Substances 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
239000002532 enzyme inhibitor Substances 0.000 description 1
CCIVGXIOQKPBKL-UHFFFAOYSA-M ethanesulfonate Chemical compound CCS([O-])(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-M 0.000 description 1
238000002270 exclusion chromatography Methods 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
210000000416 exudates and transudate Anatomy 0.000 description 1
210000003608 fece Anatomy 0.000 description 1
238000000799 fluorescence microscopy Methods 0.000 description 1
238000012757 fluorescence staining Methods 0.000 description 1
235000013305 food Nutrition 0.000 description 1
238000007710 freezing Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
208000016361 genetic disease Diseases 0.000 description 1
210000004392 genitalia Anatomy 0.000 description 1
229940116332 glucose oxidase Drugs 0.000 description 1
235000019420 glucose oxidase Nutrition 0.000 description 1
230000036541 health Effects 0.000 description 1
229930195733 hydrocarbon Natural products 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000003100 immobilizing effect Effects 0.000 description 1
230000016784 immunoglobulin production Effects 0.000 description 1
239000003547 immunosorbent Substances 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
238000013383 initial experiment Methods 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
238000002955 isolation Methods 0.000 description 1
239000002523 lectin Substances 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
239000002502 liposome Substances 0.000 description 1
210000004072 lung Anatomy 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
230000008018 melting Effects 0.000 description 1
238000002844 melting Methods 0.000 description 1
239000012528 membrane Substances 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
108010077055 methylated bovine serum albumin Proteins 0.000 description 1
108091005592 methylated proteins Proteins 0.000 description 1
YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical class CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
239000003094 microcapsule Substances 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
239000008267 milk Substances 0.000 description 1
229960004857 mitomycin Drugs 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
239000003068 molecular probe Substances 0.000 description 1
230000009871 nonspecific binding Effects 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
229940046166 oligodeoxynucleotide Drugs 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
210000000056 organ Anatomy 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
239000008188 pellet Substances 0.000 description 1
229940111202 pepsin Drugs 0.000 description 1
150000005053 phenanthridines Chemical class 0.000 description 1
150000002988 phenazines Chemical class 0.000 description 1
125000001484 phenothiazinyl group Chemical class C1(=CC=CC=2SC3=CC=CC=C3NC12)* 0.000 description 1
RHDXSLLGPJSSGW-VEGRVEBRSA-N phosphoric acid;(2r,3r,4r)-2,3,4,5-tetrahydroxypentanal Chemical group OP(O)(O)=O.OC[C@@H](O)[C@@H](O)[C@@H](O)C=O RHDXSLLGPJSSGW-VEGRVEBRSA-N 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
239000011574 phosphorus Substances 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
125000003367 polycyclic group Chemical group 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
229920000136 polysorbate Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
229920000915 polyvinyl chloride Polymers 0.000 description 1
239000004800 polyvinyl chloride Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
230000008569 process Effects 0.000 description 1
238000012545 processing Methods 0.000 description 1
125000000714 pyrimidinyl group Chemical group 0.000 description 1
150000003248 quinolines Chemical class 0.000 description 1
230000005855 radiation Effects 0.000 description 1
239000012429 reaction media Substances 0.000 description 1
238000005215 recombination Methods 0.000 description 1
230000006798 recombination Effects 0.000 description 1
230000009467 reduction Effects 0.000 description 1
230000010076 replication Effects 0.000 description 1
238000006798 ring closing metathesis reaction Methods 0.000 description 1
210000003296 saliva Anatomy 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000009738 saturating Methods 0.000 description 1
239000006152 selective media Substances 0.000 description 1
230000035939 shock Effects 0.000 description 1
208000007056 sickle cell anemia Diseases 0.000 description 1
239000007974 sodium acetate buffer Substances 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
210000001082 somatic cell Anatomy 0.000 description 1
229940063675 spermine Drugs 0.000 description 1
238000010189 synthetic method Methods 0.000 description 1
238000010257 thawing Methods 0.000 description 1
125000003396 thiol group Chemical group [H]S* 0.000 description 1
150000003573 thiols Chemical class 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
238000004448 titration Methods 0.000 description 1
239000012581 transferrin Substances 0.000 description 1
230000009466 transformation Effects 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
238000005406 washing Methods 0.000 description 1