NO820610L - Fremgangsmaate for aa oeke produksjonen av protein i vertsorganismer og dna-plasmid for utoevelse av fremgangsmaaten - Google Patents
Fremgangsmaate for aa oeke produksjonen av protein i vertsorganismer og dna-plasmid for utoevelse av fremgangsmaatenInfo
- Publication number
- NO820610L NO820610L NO820610A NO820610A NO820610L NO 820610 L NO820610 L NO 820610L NO 820610 A NO820610 A NO 820610A NO 820610 A NO820610 A NO 820610A NO 820610 L NO820610 L NO 820610L
- Authority
- NO
- Norway
- Prior art keywords
- plasmid
- gene
- site
- copy number
- repressor
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims description 65
- 108090000623 proteins and genes Proteins 0.000 title claims description 47
- 238000000034 method Methods 0.000 title claims description 23
- 102000004169 proteins and genes Human genes 0.000 title claims description 22
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- 230000010076 replication Effects 0.000 claims description 28
- 230000000977 initiatory effect Effects 0.000 claims description 13
- 238000013518 transcription Methods 0.000 claims description 12
- 230000035897 transcription Effects 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000013599 cloning vector Substances 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- 230000002103 transcriptional effect Effects 0.000 claims description 5
- 238000010367 cloning Methods 0.000 claims description 4
- 239000013598 vector Substances 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 238000013519 translation Methods 0.000 claims description 3
- 230000002950 deficient Effects 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 25
- 210000004027 cell Anatomy 0.000 description 14
- 239000013615 primer Substances 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
- 238000012217 deletion Methods 0.000 description 6
- 230000037430 deletion Effects 0.000 description 6
- 230000014616 translation Effects 0.000 description 6
- 210000000349 chromosome Anatomy 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 239000013616 RNA primer Substances 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
- 241000702374 Enterobacteria phage fd Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108091029865 Exogenous DNA Proteins 0.000 description 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
- 108010054278 Lac Repressors Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 229940123573 Protein synthesis inhibitor Drugs 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000007 protein synthesis inhibitor Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23916581A | 1981-02-27 | 1981-02-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO820610L true NO820610L (no) | 1982-08-30 |
Family
ID=22900899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO820610A NO820610L (no) | 1981-02-27 | 1982-02-26 | Fremgangsmaate for aa oeke produksjonen av protein i vertsorganismer og dna-plasmid for utoevelse av fremgangsmaaten |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0060045A3 (ja) |
| JP (1) | JPS57172000A (ja) |
| AU (1) | AU554700B2 (ja) |
| BR (1) | BR8201015A (ja) |
| CA (1) | CA1198067A (ja) |
| DK (1) | DK85682A (ja) |
| ES (1) | ES8305415A1 (ja) |
| FI (1) | FI820688L (ja) |
| IL (1) | IL65061A (ja) |
| NO (1) | NO820610L (ja) |
| WO (1) | WO1982002901A1 (ja) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1198069A (en) * | 1982-06-08 | 1985-12-17 | David H. Gelfand | Temperature sensitive multicopy plasmids |
| HU201116B (en) * | 1982-09-16 | 1990-09-28 | Benzon As Alfred | Process for producing plasmids having uncontrolled replication conduct under given circumstances |
| CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
| NL8203716A (nl) * | 1982-09-24 | 1984-04-16 | Tno | Dna en plasmiden. |
| GB8308236D0 (en) * | 1983-03-25 | 1983-05-05 | Celltech Ltd | Dna vectors |
| US5670371A (en) * | 1983-07-15 | 1997-09-23 | Bio-Technology General Corp. | Bacterial expression of superoxide dismutase |
| US4710473A (en) * | 1983-08-10 | 1987-12-01 | Amgen, Inc. | DNA plasmids |
| HU194307B (en) * | 1983-09-16 | 1988-01-28 | Richter Gedeon Vegyeszet | Process for producing plasmidvectors of high copy number |
| DE3585170D1 (de) | 1984-03-06 | 1992-02-27 | Lilly Co Eli | Expressionsvektoren fuer rinderwachstumshormonderivate. |
| US4753886A (en) * | 1984-08-10 | 1988-06-28 | Eli Lilly And Company | Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes |
| GB2166743B (en) * | 1984-11-13 | 1989-01-05 | Solvay | Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression |
| US7998732B2 (en) | 2004-02-04 | 2011-08-16 | Merck Sharp & Dohme Corp. | Process for large scale production of plasmid DNA by E. coli fermentation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4322499A (en) * | 1978-12-22 | 1982-03-30 | The Regents Of The University Of California | Adrenocorticotropin-lipotropin precursor gene |
| FI793884A (fi) * | 1978-12-26 | 1980-06-27 | Cetus Corp | Stabila plasmider med stort kopieantal |
-
1982
- 1982-02-15 CA CA000396246A patent/CA1198067A/en not_active Expired
- 1982-02-19 IL IL65061A patent/IL65061A/xx unknown
- 1982-02-22 EP EP82300865A patent/EP0060045A3/en not_active Withdrawn
- 1982-02-26 DK DK85682A patent/DK85682A/da not_active Application Discontinuation
- 1982-02-26 FI FI820688A patent/FI820688L/fi not_active Application Discontinuation
- 1982-02-26 BR BR8201015A patent/BR8201015A/pt unknown
- 1982-02-26 JP JP57030440A patent/JPS57172000A/ja active Pending
- 1982-02-26 ES ES509946A patent/ES8305415A1/es not_active Expired
- 1982-02-26 NO NO820610A patent/NO820610L/no unknown
- 1982-03-01 WO PCT/US1982/000246 patent/WO1982002901A1/en unknown
- 1982-03-01 AU AU82747/82A patent/AU554700B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO1982002901A1 (en) | 1982-09-02 |
| IL65061A (en) | 1986-01-31 |
| EP0060045A3 (en) | 1983-08-24 |
| BR8201015A (pt) | 1983-01-04 |
| AU554700B2 (en) | 1986-08-28 |
| FI820688L (fi) | 1982-08-28 |
| ES509946A0 (es) | 1983-04-01 |
| DK85682A (da) | 1982-08-28 |
| IL65061A0 (en) | 1982-04-30 |
| EP0060045A2 (en) | 1982-09-15 |
| AU8274782A (en) | 1982-09-14 |
| JPS57172000A (en) | 1982-10-22 |
| CA1198067A (en) | 1985-12-17 |
| ES8305415A1 (es) | 1983-04-01 |
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