NO332092B1 - Fremgangsmate for proteinekspresjon, anvendelse av neomycinresistensgenprodukt, samt cellekultur med hoy tetthet - Google Patents

Fremgangsmate for proteinekspresjon, anvendelse av neomycinresistensgenprodukt, samt cellekultur med hoy tetthet Download PDF

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Publication number
NO332092B1
NO332092B1 NO20041856A NO20041856A NO332092B1 NO 332092 B1 NO332092 B1 NO 332092B1 NO 20041856 A NO20041856 A NO 20041856A NO 20041856 A NO20041856 A NO 20041856A NO 332092 B1 NO332092 B1 NO 332092B1
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NO
Norway
Prior art keywords
cells
cell
density
cell culture
cell line
Prior art date
Application number
NO20041856A
Other languages
English (en)
Norwegian (no)
Other versions
NO20041856L (no
NO20041856D0 (no
Inventor
Angelo Perani
Andy Racher
John Birch
Mohamed Al-Rubeai
Original Assignee
Lonza Biologics Plc
Mohamed Al-Rubeai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Biologics Plc, Mohamed Al-Rubeai filed Critical Lonza Biologics Plc
Publication of NO20041856D0 publication Critical patent/NO20041856D0/no
Publication of NO20041856L publication Critical patent/NO20041856L/no
Publication of NO332092B1 publication Critical patent/NO332092B1/no

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/10Plasmid DNA
    • C12N2800/108Plasmid DNA episomal vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/38Vector systems having a special element relevant for transcription being a stuffer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
NO20041856A 2001-09-26 2004-04-23 Fremgangsmate for proteinekspresjon, anvendelse av neomycinresistensgenprodukt, samt cellekultur med hoy tetthet NO332092B1 (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0123098.6A GB0123098D0 (en) 2001-09-26 2001-09-26 Use of aminoglycoside resistance gene
US38759502P 2002-06-16 2002-06-16
PCT/GB2002/004522 WO2003027300A2 (en) 2001-09-26 2002-09-26 Use of aminoglycoside resistance gene

Publications (3)

Publication Number Publication Date
NO20041856D0 NO20041856D0 (no) 2004-04-23
NO20041856L NO20041856L (no) 2004-06-22
NO332092B1 true NO332092B1 (no) 2012-06-18

Family

ID=26246578

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20041856A NO332092B1 (no) 2001-09-26 2004-04-23 Fremgangsmate for proteinekspresjon, anvendelse av neomycinresistensgenprodukt, samt cellekultur med hoy tetthet

Country Status (13)

Country Link
US (1) US7439037B2 (da)
EP (1) EP1438411B1 (da)
JP (1) JP4267450B2 (da)
KR (1) KR100982153B1 (da)
CN (1) CN100402653C (da)
AT (1) ATE334215T1 (da)
AU (1) AU2002334102A1 (da)
DE (1) DE60213441T2 (da)
DK (1) DK1438411T3 (da)
ES (1) ES2269807T3 (da)
GB (1) GB0123098D0 (da)
NO (1) NO332092B1 (da)
WO (1) WO2003027300A2 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2343362T4 (da) * 2006-07-14 2024-07-08 Patheon Holdings I B V Forbedret fremgangsmåde til dyrkning af celler
WO2016176275A1 (en) * 2015-04-27 2016-11-03 Momenta Pharmaceuticals, Inc. Methods of manufacturing therapeutic proteins

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024947A (en) 1987-07-24 1991-06-18 Cetus Corporation Serum free media for the growth on insect cells and expression of products thereby
US5077214A (en) * 1989-07-07 1991-12-31 The Texas A&M University System Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
WO1994026087A2 (en) * 1993-05-14 1994-11-24 Connor Kim C O Recombinant protein production and insect cell culture and process
FR2708284B1 (fr) * 1993-07-26 1995-10-20 Agronomique Inst Nat Rech Séquences nucléotidiques permettant la réplication et le maintien d'une information génétique dans les cellules animales.
GB2289946B (en) 1994-05-26 1998-09-23 Food Industry Res & Dev Inst Method
KR20010069066A (ko) * 2000-01-12 2001-07-23 이종원 저산소 농도하에서 생존이 가능하도록 하는 동물세포의배양방법

Also Published As

Publication number Publication date
WO2003027300A2 (en) 2003-04-03
EP1438411A2 (en) 2004-07-21
KR20040054698A (ko) 2004-06-25
CN100402653C (zh) 2008-07-16
CN1705749A (zh) 2005-12-07
DE60213441D1 (de) 2006-09-07
US7439037B2 (en) 2008-10-21
US20050112722A1 (en) 2005-05-26
JP2005518189A (ja) 2005-06-23
NO20041856L (no) 2004-06-22
JP4267450B2 (ja) 2009-05-27
WO2003027300A3 (en) 2003-08-21
ATE334215T1 (de) 2006-08-15
AU2002334102A1 (en) 2003-04-07
KR100982153B1 (ko) 2010-09-14
EP1438411B1 (en) 2006-07-26
NO20041856D0 (no) 2004-04-23
ES2269807T3 (es) 2007-04-01
DE60213441T2 (de) 2007-12-13
GB0123098D0 (en) 2001-11-14
DK1438411T3 (da) 2006-11-20

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NO332092B1 (no) Fremgangsmate for proteinekspresjon, anvendelse av neomycinresistensgenprodukt, samt cellekultur med hoy tetthet
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