NO323157B1 - Oligonukleotidprimerpar, sett av oligonukleotidprimere, sett for a pavise HIV-1 nukleinsyre samt fremgangsmate for a amplifisere HIV-1 nukleinsyre - Google Patents
Oligonukleotidprimerpar, sett av oligonukleotidprimere, sett for a pavise HIV-1 nukleinsyre samt fremgangsmate for a amplifisere HIV-1 nukleinsyre Download PDFInfo
- Publication number
- NO323157B1 NO323157B1 NO19980212A NO980212A NO323157B1 NO 323157 B1 NO323157 B1 NO 323157B1 NO 19980212 A NO19980212 A NO 19980212A NO 980212 A NO980212 A NO 980212A NO 323157 B1 NO323157 B1 NO 323157B1
- Authority
- NO
- Norway
- Prior art keywords
- hiv
- seq
- nucleic acid
- primers
- primer
- Prior art date
Links
- 241000713772 Human immunodeficiency virus 1 Species 0.000 title claims abstract description 95
- 238000000034 method Methods 0.000 title claims abstract description 48
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 45
- 239000013615 primer Substances 0.000 title claims description 97
- 108020004707 nucleic acids Proteins 0.000 title claims description 41
- 102000039446 nucleic acids Human genes 0.000 title claims description 41
- 108091034117 Oligonucleotide Proteins 0.000 title claims description 18
- 239000003155 DNA primer Substances 0.000 title claims description 16
- 238000010298 pulverizing process Methods 0.000 title 1
- 208000031886 HIV Infections Diseases 0.000 claims abstract description 82
- 238000003752 polymerase chain reaction Methods 0.000 claims abstract description 27
- 230000003321 amplification Effects 0.000 claims abstract description 25
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 25
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 241000560067 HIV-1 group M Species 0.000 abstract description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 5
- 108700004026 gag Genes Proteins 0.000 abstract description 3
- 101150098622 gag gene Proteins 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 38
- 238000006243 chemical reaction Methods 0.000 description 33
- 238000012360 testing method Methods 0.000 description 28
- 239000000047 product Substances 0.000 description 25
- 238000009396 hybridization Methods 0.000 description 23
- 108020004414 DNA Proteins 0.000 description 22
- 239000003153 chemical reaction reagent Substances 0.000 description 14
- 230000000295 complement effect Effects 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 230000027455 binding Effects 0.000 description 9
- 239000011541 reaction mixture Substances 0.000 description 8
- 238000011144 upstream manufacturing Methods 0.000 description 8
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
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- 239000012528 membrane Substances 0.000 description 5
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- -1 ids Species 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
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- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 230000006820 DNA synthesis Effects 0.000 description 3
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 3
- 238000002820 assay format Methods 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 239000002751 oligonucleotide probe Substances 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000001226 triphosphate Substances 0.000 description 3
- 235000011178 triphosphate Nutrition 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 108091033380 Coding strand Proteins 0.000 description 2
- 230000004568 DNA-binding Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 241000276498 Pollachius virens Species 0.000 description 2
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 238000000099 in vitro assay Methods 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001566 pro-viral effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 208000030507 AIDS Diseases 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 240000002834 Paulownia tomentosa Species 0.000 description 1
- 235000010678 Paulownia tomentosa Nutrition 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- KDCGOANMDULRCW-UHFFFAOYSA-N Purine Natural products N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- SWPYNTWPIAZGLT-UHFFFAOYSA-N [amino(ethoxy)phosphanyl]oxyethane Chemical compound CCOP(N)OCC SWPYNTWPIAZGLT-UHFFFAOYSA-N 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 239000007998 bicine buffer Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
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- 238000010893 electron trap Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
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- 238000002372 labelling Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
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- 238000005457 optimization Methods 0.000 description 1
- 101150078841 pan gene Proteins 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- IGFXRKMLLMBKSA-UHFFFAOYSA-N purine Chemical compound N1=C[N]C2=NC=NC2=C1 IGFXRKMLLMBKSA-UHFFFAOYSA-N 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Virology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- AIDS & HIV (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Dental Preparations (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3774497P | 1997-01-17 | 1997-01-17 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO980212D0 NO980212D0 (no) | 1998-01-16 |
| NO980212L NO980212L (no) | 1998-07-20 |
| NO323157B1 true NO323157B1 (no) | 2007-01-08 |
Family
ID=21896067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19980212A NO323157B1 (no) | 1997-01-17 | 1998-01-16 | Oligonukleotidprimerpar, sett av oligonukleotidprimere, sett for a pavise HIV-1 nukleinsyre samt fremgangsmate for a amplifisere HIV-1 nukleinsyre |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5908743A (zh) |
| EP (1) | EP0854197B1 (zh) |
| JP (1) | JP2838084B2 (zh) |
| KR (1) | KR100257438B1 (zh) |
| CN (1) | CN1208469C (zh) |
| AT (1) | ATE345400T1 (zh) |
| AU (1) | AU693066B1 (zh) |
| BR (1) | BRPI9800337B8 (zh) |
| CA (1) | CA2222769C (zh) |
| CZ (1) | CZ288064B6 (zh) |
| DE (1) | DE69836395T2 (zh) |
| DK (1) | DK0854197T3 (zh) |
| ES (1) | ES2276436T3 (zh) |
| HK (1) | HK1010896A1 (zh) |
| HU (1) | HU223384B1 (zh) |
| IL (1) | IL122906A (zh) |
| NO (1) | NO323157B1 (zh) |
| PL (1) | PL191989B1 (zh) |
| RU (1) | RU2186112C2 (zh) |
| TW (1) | TW400386B (zh) |
| UA (1) | UA47432C2 (zh) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19850186A1 (de) * | 1998-10-30 | 2000-05-25 | Roche Diagnostics Gmbh | Neue Primer und Sonden zum Nachweis von HIV |
| DE19900511C2 (de) | 1999-01-08 | 2001-03-15 | Deutsches Krebsforsch | Molekularbiologische Marker für die analytische Elektronenmikroskopie |
| US6245511B1 (en) * | 1999-02-22 | 2001-06-12 | Vialogy Corp | Method and apparatus for exponentially convergent therapy effectiveness monitoring using DNA microarray based viral load measurements |
| US6582920B2 (en) | 2000-09-01 | 2003-06-24 | Gen-Probe Incorporated | Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations |
| ATE414794T1 (de) * | 2000-09-01 | 2008-12-15 | Gen Probe Inc | Amplifizierung von hiv-1 sequenzen zur detektion von sequenzen, die mit wirkstoff-resistenz mutationen einhergehen |
| US6770752B2 (en) | 2001-01-09 | 2004-08-03 | Becton, Dickinson And Company | Sequences for detection of HIV-1 |
| EP1317960A1 (en) * | 2001-12-06 | 2003-06-11 | PamGene B.V. | System and method for analyzing a blood sample and disposable cartridge for use in this system or method |
| WO2004094986A2 (en) * | 2003-04-16 | 2004-11-04 | Handylab, Inc. | System and method for electrochemical detection of biological compounds |
| WO2006130165A2 (en) | 2004-09-02 | 2006-12-07 | Vialogy Corp. | Detecting events of interest using quantum resonance interferometry |
| EP2742951B1 (en) | 2004-09-14 | 2016-02-03 | Argos Therapeutics, Inc. | Strain independent amplification of HIV polynucleotides |
| CN100340673C (zh) * | 2005-02-03 | 2007-10-03 | 上海科华生物工程股份有限公司 | 人类免疫缺陷病毒hiv－1核酸扩增－荧光定量检测试剂盒 |
| CN101144771B (zh) * | 2006-09-11 | 2012-05-30 | 中山大学达安基因股份有限公司 | 检测人类免疫缺陷病毒的试剂盒 |
| EP2574681B1 (en) | 2007-03-28 | 2016-03-23 | Signal Diagnostics | System and method for high resolution analysis of nucleic acids to detect sequence variations |
| WO2009017743A2 (en) | 2007-07-30 | 2009-02-05 | Argos Therapeutics, Inc. | Improved primers and probes for the amplification and detection of hiv gag, rev and nef polynucleotides |
| US10669574B2 (en) | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
| US10113206B2 (en) * | 2010-02-24 | 2018-10-30 | Grifols Therapeutics Inc. | Methods, compositions, and kits for determining human immunodeficiency virus (HIV) |
| RU2584573C2 (ru) * | 2011-06-15 | 2016-05-20 | Грайфолз Терепьютикс Инк. | Способы, композиции и наборы для определения вируса иммунодефицита человека (вич) |
| RU2016117929A (ru) | 2013-10-09 | 2017-11-15 | Флюоресентрик, Инк. | Мультиплексные зонды |
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| US5386022A (en) * | 1985-03-28 | 1995-01-31 | Hoffman-La Roche Inc. | Primes and probes for the amplification and detection of aids associated nucleic acids |
| US5389512A (en) * | 1988-10-07 | 1995-02-14 | Hoffman-La Roche Inc. | Method for determining the relative amount of a viral nucleic acid segment in a sample by the polymerase chain reaction |
| US5695926A (en) * | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
| JP4108118B2 (ja) * | 1993-03-26 | 2008-06-25 | ジェン−プローブ・インコーポレイテッド | ヒト・免疫不全ウイルス１型の検出 |
| AU689789B2 (en) * | 1993-07-23 | 1998-04-09 | Gen-Probe Incorporated | Methods for enhancing nucleic acid amplification |
| WO1995014109A1 (en) * | 1993-11-19 | 1995-05-26 | U.S. Department Of The Army | High through-put quantitative polymerase chain reaction for hiv clinical specimens |
| US5665545A (en) * | 1994-11-28 | 1997-09-09 | Akzo Nobel N.V. | Terminal repeat amplification method |
| US5599662A (en) * | 1995-02-17 | 1997-02-04 | Hoffmann-La Roche Inc. | Oliconucleotide primers and probes for the detection of HIV-1 |
| US5702926A (en) * | 1996-08-22 | 1997-12-30 | Becton, Dickinson And Company | Nicking of DNA using boronated nucleotides |
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