NO320586B1 - Fremgangsmate for kovalent kopling av et antistoffmolekyl, et fragment derav eller et antigenmolekyl til en fast fase. - Google Patents
Fremgangsmate for kovalent kopling av et antistoffmolekyl, et fragment derav eller et antigenmolekyl til en fast fase. Download PDFInfo
- Publication number
- NO320586B1 NO320586B1 NO19992781A NO992781A NO320586B1 NO 320586 B1 NO320586 B1 NO 320586B1 NO 19992781 A NO19992781 A NO 19992781A NO 992781 A NO992781 A NO 992781A NO 320586 B1 NO320586 B1 NO 320586B1
- Authority
- NO
- Norway
- Prior art keywords
- solid phase
- antibody
- antigen
- fragment
- polyvinyl
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 35
- 108091007433 antigens Proteins 0.000 title claims abstract description 34
- 102000036639 antigens Human genes 0.000 title claims abstract description 34
- 238000005859 coupling reaction Methods 0.000 title claims abstract description 25
- 239000007790 solid phase Substances 0.000 title claims abstract description 23
- 238000010168 coupling process Methods 0.000 title claims abstract description 22
- 230000008878 coupling Effects 0.000 title claims abstract description 19
- 239000012634 fragment Substances 0.000 title claims abstract description 19
- 239000003945 anionic surfactant Substances 0.000 claims abstract description 9
- 239000002245 particle Substances 0.000 claims description 40
- 239000000203 mixture Substances 0.000 claims description 15
- 229920000642 polymer Polymers 0.000 claims description 10
- 238000004458 analytical method Methods 0.000 claims description 9
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 6
- 229920002554 vinyl polymer Polymers 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 5
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims description 4
- 229920001577 copolymer Polymers 0.000 claims description 4
- 239000004800 polyvinyl chloride Substances 0.000 claims description 4
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 239000003929 acidic solution Substances 0.000 claims description 3
- 230000002378 acidificating effect Effects 0.000 claims description 3
- 125000004218 chloromethyl group Chemical group [H]C([H])(Cl)* 0.000 claims description 3
- 239000011258 core-shell material Substances 0.000 claims description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- KETWBQOXTBGBBN-UHFFFAOYSA-N hex-1-enylbenzene Chemical compound CCCCC=CC1=CC=CC=C1 KETWBQOXTBGBBN-UHFFFAOYSA-N 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 125000004170 methylsulfonyl group Chemical group [H]C([H])([H])S(*)(=O)=O 0.000 claims description 3
- 229920003227 poly(N-vinyl carbazole) Polymers 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 229920001447 polyvinyl benzene Polymers 0.000 claims description 3
- 229920002102 polyvinyl toluene Polymers 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- 125000002088 tosyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1C([H])([H])[H])S(*)(=O)=O 0.000 claims description 3
- YPDJUUNZSDFFBR-UHFFFAOYSA-N 1-chloroprop-2-enylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC(Cl)C1=CC=CC=C1 YPDJUUNZSDFFBR-UHFFFAOYSA-N 0.000 claims description 2
- 239000004593 Epoxy Substances 0.000 claims description 2
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical group ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 claims description 2
- 229920001519 homopolymer Polymers 0.000 claims description 2
- 238000003317 immunochromatography Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 claims description 2
- 239000003637 basic solution Substances 0.000 claims 2
- 125000000524 functional group Chemical group 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract description 6
- 238000007039 two-step reaction Methods 0.000 abstract description 2
- 239000012071 phase Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 239000000725 suspension Substances 0.000 description 7
- 108010074051 C-Reactive Protein Proteins 0.000 description 6
- 102100032752 C-reactive protein Human genes 0.000 description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 4
- 108010075210 streptolysin O Proteins 0.000 description 4
- 239000004471 Glycine Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 230000008105 immune reaction Effects 0.000 description 3
- 239000004816 latex Substances 0.000 description 3
- 229920000126 latex Polymers 0.000 description 3
- 238000006386 neutralization reaction Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241000194017 Streptococcus Species 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 239000003093 cationic surfactant Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000004848 nephelometry Methods 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- YXMISKNUHHOXFT-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) prop-2-enoate Chemical group C=CC(=O)ON1C(=O)CCC1=O YXMISKNUHHOXFT-UHFFFAOYSA-N 0.000 description 1
- JECYNCQXXKQDJN-UHFFFAOYSA-N 2-(2-methylhexan-2-yloxymethyl)oxirane Chemical compound CCCCC(C)(C)OCC1CO1 JECYNCQXXKQDJN-UHFFFAOYSA-N 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 206010018364 Glomerulonephritis Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 206010034839 Pharyngitis streptococcal Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 208000033809 Suppuration Diseases 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 206010048629 Wound secretion Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229940098197 human immunoglobulin g Drugs 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011221 initial treatment Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000002563 ionic surfactant Substances 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 210000004910 pleural fluid Anatomy 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 201000003068 rheumatic fever Diseases 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000012536 storage buffer Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 239000002888 zwitterionic surfactant Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/823—Immunogenic carrier or carrier per se
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Detergent Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI965066A FI101543B (fi) | 1996-12-17 | 1996-12-17 | Biomolekyylien kytkeminen kiintofaasiin |
| PCT/FI1997/000774 WO1998027429A1 (en) | 1996-12-17 | 1997-12-10 | Method of coupling ligands to a solid phase in acidic solution and anionic surfactant |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO992781D0 NO992781D0 (no) | 1999-06-08 |
| NO992781L NO992781L (no) | 1999-06-08 |
| NO320586B1 true NO320586B1 (no) | 2005-12-27 |
Family
ID=8547295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19992781A NO320586B1 (no) | 1996-12-17 | 1999-06-08 | Fremgangsmate for kovalent kopling av et antistoffmolekyl, et fragment derav eller et antigenmolekyl til en fast fase. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US6358753B1 (fi) |
| EP (1) | EP0946871B1 (fi) |
| JP (1) | JP3845118B2 (fi) |
| AT (1) | ATE221199T1 (fi) |
| AU (1) | AU732232B2 (fi) |
| CA (1) | CA2274956C (fi) |
| DE (1) | DE69714291T2 (fi) |
| DK (1) | DK0946871T3 (fi) |
| ES (1) | ES2179376T3 (fi) |
| FI (1) | FI101543B (fi) |
| IL (1) | IL130459A (fi) |
| NO (1) | NO320586B1 (fi) |
| WO (1) | WO1998027429A1 (fi) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4703018A (en) | 1985-02-20 | 1987-10-27 | E. I. Du Pont De Nemours And Company | High refractive index haloalkyl-functional shell-core polymers and their use in light scattering immunoassays |
| EP0257758B1 (en) | 1986-08-07 | 1993-03-10 | Minnesota Mining And Manufacturing Company | Stable biologically active fluorochemical emulsions |
| US5585278A (en) | 1994-10-27 | 1996-12-17 | Bayer Corporation | Method for coupling antibody to novel latex substrate and its use in immunoassay |
-
1996
- 1996-12-17 FI FI965066A patent/FI101543B/fi not_active IP Right Cessation
-
1997
- 1997-12-10 IL IL13045997A patent/IL130459A/en not_active IP Right Cessation
- 1997-12-10 EP EP97947054A patent/EP0946871B1/en not_active Expired - Lifetime
- 1997-12-10 CA CA002274956A patent/CA2274956C/en not_active Expired - Lifetime
- 1997-12-10 JP JP52736098A patent/JP3845118B2/ja not_active Expired - Lifetime
- 1997-12-10 ES ES97947054T patent/ES2179376T3/es not_active Expired - Lifetime
- 1997-12-10 AT AT97947054T patent/ATE221199T1/de active
- 1997-12-10 WO PCT/FI1997/000774 patent/WO1998027429A1/en active IP Right Grant
- 1997-12-10 DK DK97947054T patent/DK0946871T3/da active
- 1997-12-10 AU AU52240/98A patent/AU732232B2/en not_active Expired
- 1997-12-10 US US09/331,056 patent/US6358753B1/en not_active Expired - Lifetime
- 1997-12-10 DE DE69714291T patent/DE69714291T2/de not_active Expired - Lifetime
-
1999
- 1999-06-08 NO NO19992781A patent/NO320586B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO992781D0 (no) | 1999-06-08 |
| FI101543B1 (fi) | 1998-07-15 |
| JP2001509887A (ja) | 2001-07-24 |
| DE69714291D1 (de) | 2002-08-29 |
| JP3845118B2 (ja) | 2006-11-15 |
| EP0946871A1 (en) | 1999-10-06 |
| WO1998027429A1 (en) | 1998-06-25 |
| AU732232B2 (en) | 2001-04-12 |
| ATE221199T1 (de) | 2002-08-15 |
| CA2274956C (en) | 2007-07-03 |
| CA2274956A1 (en) | 1998-06-25 |
| DK0946871T3 (da) | 2002-11-04 |
| DE69714291T2 (de) | 2003-03-20 |
| FI101543B (fi) | 1998-07-15 |
| IL130459A0 (en) | 2000-06-01 |
| ES2179376T3 (es) | 2003-01-16 |
| NO992781L (no) | 1999-06-08 |
| EP0946871B1 (en) | 2002-07-24 |
| IL130459A (en) | 2002-07-25 |
| US6358753B1 (en) | 2002-03-19 |
| FI965066A0 (fi) | 1996-12-17 |
| AU5224098A (en) | 1998-07-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK1K | Patent expired |