NO309153B1 - Fremgangsmåte for ökning ved sekresjon av disulfidbrodannede proteiner - Google Patents
Fremgangsmåte for ökning ved sekresjon av disulfidbrodannede proteiner Download PDFInfo
- Publication number
- NO309153B1 NO309153B1 NO921574A NO921574A NO309153B1 NO 309153 B1 NO309153 B1 NO 309153B1 NO 921574 A NO921574 A NO 921574A NO 921574 A NO921574 A NO 921574A NO 309153 B1 NO309153 B1 NO 309153B1
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- Norway
- Prior art keywords
- protein
- thiol reagents
- recombinant dna
- expression
- nutrient medium
- Prior art date
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 64
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 42
- 230000028327 secretion Effects 0.000 title claims abstract description 13
- 230000001965 increasing effect Effects 0.000 title claims description 6
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 title description 3
- 239000003774 sulfhydryl reagent Substances 0.000 claims abstract description 36
- 230000014509 gene expression Effects 0.000 claims abstract description 26
- 108020004511 Recombinant DNA Proteins 0.000 claims abstract description 25
- 235000015097 nutrients Nutrition 0.000 claims abstract description 25
- 235000018102 proteins Nutrition 0.000 claims description 49
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims description 43
- 108020003519 protein disulfide isomerase Proteins 0.000 claims description 25
- 241000588724 Escherichia coli Species 0.000 claims description 19
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 18
- 229960003180 glutathione Drugs 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 14
- 108010024636 Glutathione Proteins 0.000 claims description 13
- 230000015572 biosynthetic process Effects 0.000 claims description 12
- 108010079246 OMPA outer membrane proteins Proteins 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 108020004414 DNA Proteins 0.000 claims description 6
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims description 6
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- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 claims description 2
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- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims description 2
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- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
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- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
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- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
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- 241000702208 Shigella phage SfX Species 0.000 description 1
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- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000003392 amylase inhibitor Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000006664 bond formation reaction Methods 0.000 description 1
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- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
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- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
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- 229940053128 nerve growth factor Drugs 0.000 description 1
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- 125000003729 nucleotide group Chemical group 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4113750A DE4113750A1 (de) | 1991-04-26 | 1991-04-26 | Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO921574D0 NO921574D0 (no) | 1992-04-23 |
| NO921574L NO921574L (no) | 1992-10-27 |
| NO309153B1 true NO309153B1 (no) | 2000-12-18 |
Family
ID=6430469
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO921574A NO309153B1 (no) | 1991-04-26 | 1992-04-23 | Fremgangsmåte for ökning ved sekresjon av disulfidbrodannede proteiner |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US6333175B1 (ja) |
| EP (1) | EP0510658B1 (ja) |
| JP (1) | JPH0753118B2 (ja) |
| KR (1) | KR960002869B1 (ja) |
| AT (1) | ATE109205T1 (ja) |
| AU (1) | AU636537B2 (ja) |
| CA (1) | CA2066370C (ja) |
| DE (2) | DE4113750A1 (ja) |
| DK (1) | DK0510658T3 (ja) |
| ES (1) | ES2057944T3 (ja) |
| FI (1) | FI104262B1 (ja) |
| IE (1) | IE65792B1 (ja) |
| IL (1) | IL101686A (ja) |
| NO (1) | NO309153B1 (ja) |
| NZ (1) | NZ242492A (ja) |
| ZA (1) | ZA922971B (ja) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0547102T3 (da) * | 1990-09-05 | 1999-04-19 | Natinco Nv | Opløseliggørelse af proteiner på aktiv form |
| US6291205B1 (en) * | 1992-06-12 | 2001-09-18 | Merck & Co., Inc. | Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae |
| US5639635A (en) * | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5789199A (en) * | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US6027888A (en) * | 1996-04-05 | 2000-02-22 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells |
| US6083715A (en) * | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| US20080076706A1 (en) | 1997-07-14 | 2008-03-27 | Bolder Biotechnology, Inc. | Derivatives of Growth Hormone and Related Proteins, and Methods of Use Thereof |
| US6753165B1 (en) | 1999-01-14 | 2004-06-22 | Bolder Biotechnology, Inc. | Methods for making proteins containing free cysteine residues |
| EP1881005B1 (en) | 1997-07-14 | 2013-04-03 | Bolder Biotechnology, Inc. | Derivatives of G-CSF and related proteins |
| US7153943B2 (en) | 1997-07-14 | 2006-12-26 | Bolder Biotechnology, Inc. | Derivatives of growth hormone and related proteins, and methods of use thereof |
| US7495087B2 (en) | 1997-07-14 | 2009-02-24 | Bolder Biotechnology, Inc. | Cysteine muteins in the C-D loop of human interleukin-11 |
| US8288126B2 (en) | 1999-01-14 | 2012-10-16 | Bolder Biotechnology, Inc. | Methods for making proteins containing free cysteine residues |
| BR0008759B1 (pt) * | 1999-01-14 | 2014-03-11 | Bolder Biotechnology Inc | Métodos para a produção de proteinas contendo resíduos de cisteina livre |
| EP1048732A1 (de) * | 1999-04-26 | 2000-11-02 | F. Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen |
| EP1077263A1 (de) | 1999-07-29 | 2001-02-21 | F.Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen |
| US20040203132A1 (en) | 2003-02-28 | 2004-10-14 | Cognetix, Inc. | Conus protein disulfide isomerase |
| US7244616B2 (en) | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| KR20050070512A (ko) * | 2003-12-30 | 2005-07-07 | 삼성정밀화학 주식회사 | 재조합 단백질의 생산성을 증가시키는 무혈청 배지 및동물세포 배양방법 |
| EP1805320B1 (en) | 2004-10-22 | 2014-09-03 | Amgen Inc. | Methods for refolding of recombinant antibodies |
| EP2004804A2 (en) * | 2006-04-07 | 2008-12-24 | Dow Global Technologies Inc. | Processes for improved disulfide bond formation in recombinant systems |
| KR100743973B1 (ko) * | 2006-04-27 | 2007-07-30 | 한국과학기술원 | 목적단백질 및 코펙터를 재생시키는 효소를 동시에 세포표면에 표면발현시키는 방법 |
| GB201101794D0 (en) | 2011-02-02 | 2011-03-16 | Fermentas Uab | Protein production |
| US9944960B2 (en) * | 2015-11-17 | 2018-04-17 | Premier Research Labs, Lp | Process for microbial production of dihydrolipoic acid and extraction of dihydrolipoic acid with edible oils |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3537708A1 (de) * | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
| US4904602A (en) * | 1985-11-27 | 1990-02-27 | Repligen Corporation | Thioredoxin shufflease and use thereof |
| IL86455A0 (en) * | 1987-06-01 | 1988-11-15 | Takeda Chemical Industries Ltd | Polypeptide and production thereof |
| DE3851685D1 (de) * | 1987-10-13 | 1994-11-03 | Thomae Gmbh Dr K | Verfahren zur Herstellung von Proteinen. |
| DE3813107A1 (de) | 1988-04-19 | 1989-11-02 | Consortium Elektrochem Ind | Sekretormutante von escherichia coli |
| DE69213714T2 (de) * | 1991-01-21 | 1997-01-23 | Genzyme Corp., Cambridge, Mass. | Herstellung enzymatischer aktiver glukocerebrosidase von rekombinanten zellen |
-
1991
- 1991-04-26 DE DE4113750A patent/DE4113750A1/de not_active Withdrawn
-
1992
- 1992-04-16 CA CA002066370A patent/CA2066370C/en not_active Expired - Lifetime
- 1992-04-22 IE IE921291A patent/IE65792B1/en not_active IP Right Cessation
- 1992-04-23 IL IL10168692A patent/IL101686A/xx unknown
- 1992-04-23 DK DK92106978.7T patent/DK0510658T3/da active
- 1992-04-23 DE DE59200312T patent/DE59200312D1/de not_active Expired - Lifetime
- 1992-04-23 AT AT92106978T patent/ATE109205T1/de not_active IP Right Cessation
- 1992-04-23 ES ES92106978T patent/ES2057944T3/es not_active Expired - Lifetime
- 1992-04-23 NO NO921574A patent/NO309153B1/no unknown
- 1992-04-23 EP EP92106978A patent/EP0510658B1/de not_active Expired - Lifetime
- 1992-04-24 AU AU15159/92A patent/AU636537B2/en not_active Ceased
- 1992-04-24 NZ NZ242492A patent/NZ242492A/xx not_active IP Right Cessation
- 1992-04-24 FI FI921838A patent/FI104262B1/fi not_active IP Right Cessation
- 1992-04-24 ZA ZA922971A patent/ZA922971B/xx unknown
- 1992-04-25 KR KR1019920007039A patent/KR960002869B1/ko not_active IP Right Cessation
- 1992-04-27 JP JP4107284A patent/JPH0753118B2/ja not_active Expired - Lifetime
-
1993
- 1993-07-27 US US08/097,621 patent/US6333175B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| IL101686A (en) | 2001-08-26 |
| ES2057944T3 (es) | 1994-10-16 |
| CA2066370C (en) | 1996-04-02 |
| FI921838A (fi) | 1992-10-27 |
| FI104262B (fi) | 1999-12-15 |
| EP0510658B1 (de) | 1994-07-27 |
| DE59200312D1 (de) | 1994-09-01 |
| US6333175B1 (en) | 2001-12-25 |
| KR920019818A (ko) | 1992-11-20 |
| ATE109205T1 (de) | 1994-08-15 |
| IE921291A1 (en) | 1992-11-04 |
| JPH05268983A (ja) | 1993-10-19 |
| DK0510658T3 (da) | 1994-11-28 |
| IL101686A0 (en) | 1992-12-30 |
| AU1515992A (en) | 1992-11-19 |
| IE65792B1 (en) | 1995-11-29 |
| NZ242492A (en) | 1993-09-27 |
| CA2066370A1 (en) | 1992-10-27 |
| FI921838A0 (fi) | 1992-04-24 |
| NO921574L (no) | 1992-10-27 |
| NO921574D0 (no) | 1992-04-23 |
| EP0510658A2 (de) | 1992-10-28 |
| EP0510658A3 (en) | 1993-05-26 |
| JPH0753118B2 (ja) | 1995-06-07 |
| KR960002869B1 (ko) | 1996-02-27 |
| AU636537B2 (en) | 1993-04-29 |
| DE4113750A1 (de) | 1992-10-29 |
| ZA922971B (en) | 1992-12-30 |
| FI104262B1 (fi) | 1999-12-15 |
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