NL9301272A - Chicken Anemia Virus mutanten en vaccins en toepassingen gebaseerd op de virale eiwitten VP1, VP2 en VP3 of daarvoor coderende sequenties van dat virus. - Google Patents
Chicken Anemia Virus mutanten en vaccins en toepassingen gebaseerd op de virale eiwitten VP1, VP2 en VP3 of daarvoor coderende sequenties van dat virus. Download PDFInfo
- Publication number
- NL9301272A NL9301272A NL9301272A NL9301272A NL9301272A NL 9301272 A NL9301272 A NL 9301272A NL 9301272 A NL9301272 A NL 9301272A NL 9301272 A NL9301272 A NL 9301272A NL 9301272 A NL9301272 A NL 9301272A
- Authority
- NL
- Netherlands
- Prior art keywords
- cav
- pbs
- dna
- recombinant
- virus
- Prior art date
Links
- 241000725585 Chicken anemia virus Species 0.000 title claims abstract description 239
- 241000700605 Viruses Species 0.000 title claims abstract description 40
- 229960005486 vaccine Drugs 0.000 title abstract description 13
- 210000004027 cell Anatomy 0.000 claims abstract description 124
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 82
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 78
- 108020004414 DNA Proteins 0.000 claims abstract description 72
- 208000015181 infectious disease Diseases 0.000 claims abstract description 57
- 241001465754 Metazoa Species 0.000 claims abstract description 45
- 230000003472 neutralizing effect Effects 0.000 claims abstract description 41
- 230000006698 induction Effects 0.000 claims abstract description 18
- 239000000203 mixture Substances 0.000 claims abstract description 12
- 210000004881 tumor cell Anatomy 0.000 claims abstract description 11
- 230000030833 cell death Effects 0.000 claims abstract description 9
- 238000001514 detection method Methods 0.000 claims abstract description 9
- 108020004511 Recombinant DNA Proteins 0.000 claims abstract description 6
- 241000287828 Gallus gallus Species 0.000 claims description 54
- 241000701447 unidentified baculovirus Species 0.000 claims description 53
- 239000013598 vector Substances 0.000 claims description 33
- 239000012634 fragment Substances 0.000 claims description 28
- 230000003053 immunization Effects 0.000 claims description 24
- 238000002649 immunization Methods 0.000 claims description 24
- 239000006166 lysate Substances 0.000 claims description 22
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 22
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 18
- 229920001184 polypeptide Polymers 0.000 claims description 17
- 206010028980 Neoplasm Diseases 0.000 claims description 16
- 241000238631 Hexapoda Species 0.000 claims description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 15
- 239000002671 adjuvant Substances 0.000 claims description 15
- 230000006907 apoptotic process Effects 0.000 claims description 13
- 239000013592 cell lysate Substances 0.000 claims description 13
- 238000005534 hematocrit Methods 0.000 claims description 13
- 210000001541 thymus gland Anatomy 0.000 claims description 12
- 235000013601 eggs Nutrition 0.000 claims description 11
- 230000002829 reductive effect Effects 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 8
- 102000053602 DNA Human genes 0.000 claims description 7
- 210000002969 egg yolk Anatomy 0.000 claims description 6
- 244000144977 poultry Species 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 5
- 238000011282 treatment Methods 0.000 claims description 5
- 108010067390 Viral Proteins Proteins 0.000 claims description 4
- 230000035772 mutation Effects 0.000 claims description 4
- 238000006386 neutralization reaction Methods 0.000 claims description 4
- 230000002265 prevention Effects 0.000 claims description 4
- 230000002238 attenuated effect Effects 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 235000013345 egg yolk Nutrition 0.000 claims description 3
- 102000005962 receptors Human genes 0.000 claims description 3
- 108020003175 receptors Proteins 0.000 claims description 3
- 230000009385 viral infection Effects 0.000 claims description 3
- 239000013603 viral vector Substances 0.000 claims description 3
- 230000003612 virological effect Effects 0.000 claims description 3
- 125000001433 C-terminal amino-acid group Chemical group 0.000 claims description 2
- 102000037865 fusion proteins Human genes 0.000 claims description 2
- 108020001507 fusion proteins Proteins 0.000 claims description 2
- 239000002502 liposome Substances 0.000 claims description 2
- 230000001177 retroviral effect Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 101100191768 Caenorhabditis elegans pbs-4 gene Proteins 0.000 claims 3
- 230000010773 Antigen Neutralization Effects 0.000 claims 2
- 101100298998 Caenorhabditis elegans pbs-3 gene Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical group 0.000 claims 2
- 230000007423 decrease Effects 0.000 claims 1
- 238000004520 electroporation Methods 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- 239000000427 antigen Substances 0.000 abstract description 14
- 102000036639 antigens Human genes 0.000 abstract description 14
- 108091007433 antigens Proteins 0.000 abstract description 14
- 230000006882 induction of apoptosis Effects 0.000 abstract description 6
- 230000002163 immunogen Effects 0.000 abstract description 5
- 230000001717 pathogenic effect Effects 0.000 abstract description 5
- 230000002458 infectious effect Effects 0.000 abstract description 4
- 230000003302 anti-idiotype Effects 0.000 abstract description 2
- 238000001415 gene therapy Methods 0.000 abstract description 2
- 230000005847 immunogenicity Effects 0.000 abstract description 2
- 238000003149 assay kit Methods 0.000 abstract 1
- 101000805768 Banna virus (strain Indonesia/JKT-6423/1980) mRNA (guanine-N(7))-methyltransferase Proteins 0.000 description 114
- 101000686790 Chaetoceros protobacilladnavirus 2 Replication-associated protein Proteins 0.000 description 114
- 101000864475 Chlamydia phage 1 Internal scaffolding protein VP3 Proteins 0.000 description 114
- 101000803553 Eumenes pomiformis Venom peptide 3 Proteins 0.000 description 114
- 101000583961 Halorubrum pleomorphic virus 1 Matrix protein Proteins 0.000 description 114
- 101710081079 Minor spike protein H Proteins 0.000 description 75
- 235000013330 chicken meat Nutrition 0.000 description 52
- 101710118046 RNA-directed RNA polymerase Proteins 0.000 description 48
- 101710132601 Capsid protein Proteins 0.000 description 47
- 101710197658 Capsid protein VP1 Proteins 0.000 description 47
- 101710108545 Viral protein 1 Proteins 0.000 description 47
- 239000013612 plasmid Substances 0.000 description 14
- 238000002360 preparation method Methods 0.000 description 14
- 238000001890 transfection Methods 0.000 description 14
- 239000003446 ligand Substances 0.000 description 13
- 230000028993 immune response Effects 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 11
- 210000004940 nucleus Anatomy 0.000 description 11
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 11
- 238000012546 transfer Methods 0.000 description 11
- 108091026890 Coding region Proteins 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 230000008774 maternal effect Effects 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 8
- 238000010166 immunofluorescence Methods 0.000 description 7
- 101150066555 lacZ gene Proteins 0.000 description 7
- 210000001744 T-lymphocyte Anatomy 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 230000001681 protective effect Effects 0.000 description 6
- 238000010186 staining Methods 0.000 description 6
- 108020004705 Codon Proteins 0.000 description 5
- 108091081024 Start codon Proteins 0.000 description 5
- 208000007502 anemia Diseases 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 238000002965 ELISA Methods 0.000 description 4
- 108700026244 Open Reading Frames Proteins 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 101710121537 mRNA (guanine-N(7))-methyltransferase Proteins 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 235000013594 poultry meat Nutrition 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000002992 thymic effect Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 3
- 108091092724 Noncoding DNA Proteins 0.000 description 3
- 101710182846 Polyhedrin Proteins 0.000 description 3
- 230000001640 apoptogenic effect Effects 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 3
- 108091092356 cellular DNA Proteins 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000000120 cytopathologic effect Effects 0.000 description 3
- 238000002405 diagnostic procedure Methods 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 229960002897 heparin Drugs 0.000 description 3
- 229920000669 heparin Polymers 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 229940031626 subunit vaccine Drugs 0.000 description 3
- 101710154545 16 kDa protein Proteins 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 102100026189 Beta-galactosidase Human genes 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 2
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 208000003322 Coinfection Diseases 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- 241000701047 Gallid alphaherpesvirus 2 Species 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000037444 atrophy Effects 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000003271 compound fluorescence assay Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009585 enzyme analysis Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 229940031551 inactivated vaccine Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000004989 spleen cell Anatomy 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000711404 Avian avulavirus 1 Species 0.000 description 1
- 241000212384 Bifora Species 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 206010006811 Bursitis Diseases 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000010789 Interleukin-2 Receptors Human genes 0.000 description 1
- 108010038453 Interleukin-2 Receptors Proteins 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 208000006758 Marek Disease Diseases 0.000 description 1
- 241001502481 Meleagrid alphaherpesvirus 1 Species 0.000 description 1
- 239000012901 Milli-Q water Substances 0.000 description 1
- 208000009869 Neu-Laxova syndrome Diseases 0.000 description 1
- 208000010359 Newcastle Disease Diseases 0.000 description 1
- 108010077850 Nuclear Localization Signals Proteins 0.000 description 1
- 241000702244 Orthoreovirus Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 101710136297 Protein VP2 Proteins 0.000 description 1
- 241000714474 Rous sarcoma virus Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000256251 Spodoptera frugiperda Species 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 206010058874 Viraemia Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 101150061458 cav gene Proteins 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012412 chemical coupling Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 231100001021 decreased hematocrit Toxicity 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 206010025135 lupus erythematosus Diseases 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 230000008884 pinocytosis Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000001995 reticulocyte Anatomy 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 230000005740 tumor formation Effects 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 230000005570 vertical transmission Effects 0.000 description 1
- 210000000605 viral structure Anatomy 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/10011—Circoviridae
- C12N2750/10021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/10011—Circoviridae
- C12N2750/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Oncology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (26)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL9301272A NL9301272A (nl) | 1993-07-20 | 1993-07-20 | Chicken Anemia Virus mutanten en vaccins en toepassingen gebaseerd op de virale eiwitten VP1, VP2 en VP3 of daarvoor coderende sequenties van dat virus. |
| US08/454,121 US6071520A (en) | 1990-09-12 | 1994-07-19 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor |
| DE69435117T DE69435117D1 (de) | 1993-07-20 | 1994-07-19 | Hühner-Anaemie-Virus Mutante, Impfstoffe, und Verwendungen basiert auf Virusprotein VP3, oder dafür kodierende Virus-Sequenzen |
| PT01205103T PT1253201E (pt) | 1993-07-20 | 1994-07-19 | Mutantes e vacinas do virus da anemia da galinha e utilizações à base de proteínas virais, pv1, pv2 e pv3 ou das sequências deste vírus que asseguram a sua codificação |
| EP01205103A EP1253201B1 (en) | 1993-07-20 | 1994-07-19 | Chicken anemia virus mutants and vaccines based on the viral protein VP3 or sequences of that virus coding therefor |
| PCT/NL1994/000168 WO1995003414A2 (en) | 1993-07-20 | 1994-07-19 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins vp1, vp2 and vp3 or sequences of that virus coding therefor |
| DK01205103T DK1253201T3 (da) | 1993-07-20 | 1994-07-19 | Kyllingeanæmivirusmutanter og vacciner baseret på det virale protein VP3 eller sekvenser af dette virus kodende derfor |
| ES94925638T ES2196032T3 (es) | 1993-07-20 | 1994-07-19 | Mutantes y vacunas del virus de la anemia infecciosa de los pollos y utilizaciones basadas sobre las proteinas virales vp1, vp2 y vp3 o secuencias de dicho virus que las codifican. |
| PT94925638T PT784685E (pt) | 1993-07-20 | 1994-07-19 | Mutantes e vacinas do virus da anemia infecciosa da galinha e utilizacoes a base de proteinas viricas vp1, vp2 e vp3 ou das sequencias deste virus que asseguram a sua codificacao |
| ES01205103T ES2311005T3 (es) | 1993-07-20 | 1994-07-19 | Mutantes y vacunas del virus de la anemia infecciosa de los pollos basadas sobre la proteina viral vp3 o secuencias de dicho virus que las codifica. |
| AT94925638T ATE236980T1 (de) | 1993-07-20 | 1994-07-19 | Huehner-anaemie-virus mutante, impfstoffe, und verwendung basiert auf virusproteinen vp1, vp2 und vp3, oder dafuer kodierende virus-sequenzen |
| AT01205103T ATE401406T1 (de) | 1993-07-20 | 1994-07-19 | Hühner-anaemie-virus mutante, impfstoffe, und verwendungen basiert auf virusprotein vp3, oder dafür kodierende virus-sequenzen |
| CA002167578A CA2167578C (en) | 1993-07-20 | 1994-07-19 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins vp1, vp2 and vp3 or sequences of that virus coding therefor |
| ZA945275A ZA945275B (en) | 1993-07-20 | 1994-07-19 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2, and VP3 or sequences of that virus coding therefor |
| DK94925638T DK0784685T3 (da) | 1993-07-20 | 1994-07-19 | Hønse-anæmi-virusmutanter og vacciner og anvendelser baseret på virusproteinerne VP1, VP2 og VP3 eller derfor kodende virussekvenser |
| DE69432486T DE69432486T2 (de) | 1993-07-20 | 1994-07-19 | Huehner-anaemie-virus mutante, impfstoffe, und verwendung basiert auf virusproteinen vp1, vp2 und vp3, oder dafuer kodierende virus-sequenzen |
| AU75473/94A AU703187B2 (en) | 1993-07-20 | 1994-07-19 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor |
| JP50507295A JP3829290B2 (ja) | 1993-07-20 | 1994-07-19 | ニワトリ貧血症ウイルス変異体およびワクチン、ならびにウイルスタンパク質ｖｐ１、ｖｐ２およびｖｐ３またはウイルスをコードする配列に基づくそれらのための用途 |
| EP94925638A EP0784685B1 (en) | 1993-07-20 | 1994-07-19 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins vp1, vp2 and vp3 or sequences of that virus coding therefor |
| US08/489,666 US5922600A (en) | 1990-09-12 | 1995-06-07 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1 VP2 and VP3 or sequences of that virus coding therefor |
| US08/485,001 US5981502A (en) | 1990-09-12 | 1995-06-07 | Methods and compositions for inducing apoptosis in tumor cells |
| US08/482,161 US6162461A (en) | 1990-09-12 | 1995-06-07 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor |
| US08/911,092 US5952002A (en) | 1993-07-20 | 1997-08-14 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor |
| US09/057,963 US6217870B1 (en) | 1990-09-12 | 1998-04-09 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1 VP2 and VP3 or sequences of that virus coding therefor |
| US09/740,676 US7427658B2 (en) | 1990-09-12 | 2000-12-18 | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor |
| US11/888,039 US20080234466A1 (en) | 1990-09-12 | 2007-07-30 | Delivery method for the tumor-specific apoptosis-inducing activity of apoptin |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL9301272A NL9301272A (nl) | 1993-07-20 | 1993-07-20 | Chicken Anemia Virus mutanten en vaccins en toepassingen gebaseerd op de virale eiwitten VP1, VP2 en VP3 of daarvoor coderende sequenties van dat virus. |
| NL9301272 | 1993-07-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL9301272A true NL9301272A (nl) | 1995-02-16 |
Family
ID=19862681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL9301272A NL9301272A (nl) | 1990-09-12 | 1993-07-20 | Chicken Anemia Virus mutanten en vaccins en toepassingen gebaseerd op de virale eiwitten VP1, VP2 en VP3 of daarvoor coderende sequenties van dat virus. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5952002A (ja) |
| EP (2) | EP1253201B1 (ja) |
| JP (1) | JP3829290B2 (ja) |
| AT (2) | ATE401406T1 (ja) |
| AU (1) | AU703187B2 (ja) |
| CA (1) | CA2167578C (ja) |
| DE (2) | DE69435117D1 (ja) |
| DK (2) | DK0784685T3 (ja) |
| ES (2) | ES2311005T3 (ja) |
| NL (1) | NL9301272A (ja) |
| PT (2) | PT1253201E (ja) |
| WO (1) | WO1995003414A2 (ja) |
| ZA (1) | ZA945275B (ja) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5922600A (en) * | 1990-09-12 | 1999-07-13 | Leadd Bv | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1 VP2 and VP3 or sequences of that virus coding therefor |
| US20080234466A1 (en) * | 1990-09-12 | 2008-09-25 | Noteborn Mathieu H M | Delivery method for the tumor-specific apoptosis-inducing activity of apoptin |
| EP0872552A1 (en) * | 1997-04-15 | 1998-10-21 | Leadd B.V. | A gene delivery vehicle expressing the apoptosis-inducing proteins VP2 and/or Apoptin |
| EP0878546A1 (en) * | 1997-04-15 | 1998-11-18 | Leadd B.V. | A gene delivery vehicle expressing the apoptosis-inducing proteins VP2 and/or apoptin |
| US5789567A (en) * | 1994-07-06 | 1998-08-04 | Cornell Research Foundation, Inc. | Chicken infectious anemia virus vaccine |
| GB9414888D0 (en) * | 1994-07-23 | 1994-09-14 | Univ Belfast | Method and composition |
| EP0832242A1 (en) * | 1995-06-07 | 1998-04-01 | Aesculaap B.V. | Neutralizing conformational epitopes of chicken anemia virus |
| WO1996041191A1 (en) * | 1995-06-07 | 1996-12-19 | Aesculaap B.V. | Methods and uses for apoptin |
| EP0927757A1 (en) * | 1997-12-03 | 1999-07-07 | Leadd B.V. | Methods and means for inducing apoptosis by interfering with Bip-like proteins |
| US7361730B1 (en) * | 1999-03-15 | 2008-04-22 | University Of Maryland | Surface localized colligin/Hsp47 in carcinoma cells |
| EP1199363A1 (en) * | 2000-10-20 | 2002-04-24 | Leadd B.V. | Phosphorylation modifications of apoptin |
| AUPR567401A0 (en) | 2001-06-14 | 2001-07-12 | University Of Melbourne, The | Circovirus vaccines |
| DK2371383T3 (en) | 2005-12-29 | 2015-11-30 | Boehringer Ingelheim Vetmed | Use of a PCV2 immunogenic composition for reduction of clinical symptoms in pigs |
| MY149398A (en) | 2005-12-29 | 2013-08-30 | Boehringer Ingelheim Vetmed | Multivalent pcv2 immunogenic compositions and methods of producing such compositions |
| CN102311928B (zh) * | 2011-08-25 | 2013-04-24 | 中国农业科学院哈尔滨兽医研究所 | 鸡贫血病毒vp1、vp2蛋白共表达的重组酵母工程菌、其构建方法及应用 |
| WO2017066772A1 (en) | 2015-10-16 | 2017-04-20 | Kansas State University Research Foundation | Porcine circovirus type 3 immunogenic compositions and methods of making and using the same |
| MA43503B2 (fr) * | 2016-04-26 | 2021-02-26 | London School Of Hyghiene & Tropical Medicine | Arn viral simple brin isole (ssrna) derive du genome du virus de la peste equine (ahsv) pour la vaccination d'animaux contre le ahsv |
| CN113980146B (zh) * | 2021-11-11 | 2022-09-27 | 扬州优邦生物药品有限公司 | 三聚体化鸭黄病毒E蛋白domainIII、其制备方法和运用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992004446A1 (en) * | 1990-09-12 | 1992-03-19 | Aesculaap B.V. | Cloning of chicken anaemia dna |
| EP0483911A2 (en) * | 1990-10-31 | 1992-05-06 | Akzo N.V. | Chicken anemia virus vaccine and diagnostic reagent |
| EP0533294A1 (en) * | 1991-09-20 | 1993-03-24 | Akzo Nobel N.V. | Chicken anaemia agent vaccine |
-
1993
- 1993-07-20 NL NL9301272A patent/NL9301272A/nl not_active Application Discontinuation
-
1994
- 1994-07-19 AU AU75473/94A patent/AU703187B2/en not_active Ceased
- 1994-07-19 DK DK94925638T patent/DK0784685T3/da active
- 1994-07-19 DK DK01205103T patent/DK1253201T3/da active
- 1994-07-19 WO PCT/NL1994/000168 patent/WO1995003414A2/en active IP Right Grant
- 1994-07-19 DE DE69435117T patent/DE69435117D1/de not_active Expired - Fee Related
- 1994-07-19 JP JP50507295A patent/JP3829290B2/ja not_active Expired - Lifetime
- 1994-07-19 AT AT01205103T patent/ATE401406T1/de not_active IP Right Cessation
- 1994-07-19 DE DE69432486T patent/DE69432486T2/de not_active Expired - Fee Related
- 1994-07-19 ZA ZA945275A patent/ZA945275B/xx unknown
- 1994-07-19 PT PT01205103T patent/PT1253201E/pt unknown
- 1994-07-19 ES ES01205103T patent/ES2311005T3/es not_active Expired - Lifetime
- 1994-07-19 AT AT94925638T patent/ATE236980T1/de not_active IP Right Cessation
- 1994-07-19 PT PT94925638T patent/PT784685E/pt unknown
- 1994-07-19 EP EP01205103A patent/EP1253201B1/en not_active Expired - Lifetime
- 1994-07-19 ES ES94925638T patent/ES2196032T3/es not_active Expired - Lifetime
- 1994-07-19 CA CA002167578A patent/CA2167578C/en not_active Expired - Fee Related
- 1994-07-19 EP EP94925638A patent/EP0784685B1/en not_active Expired - Lifetime
-
1997
- 1997-08-14 US US08/911,092 patent/US5952002A/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992004446A1 (en) * | 1990-09-12 | 1992-03-19 | Aesculaap B.V. | Cloning of chicken anaemia dna |
| EP0483911A2 (en) * | 1990-10-31 | 1992-05-06 | Akzo N.V. | Chicken anemia virus vaccine and diagnostic reagent |
| EP0533294A1 (en) * | 1991-09-20 | 1993-03-24 | Akzo Nobel N.V. | Chicken anaemia agent vaccine |
Non-Patent Citations (1)
| Title |
|---|
| NOTEBORN ET AL.: "Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle", JOURNAL OF VIROLOGY, vol. 65, no. 6, June 1991 (1991-06-01), pages 3131 - 3139 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU703187B2 (en) | 1999-03-18 |
| ES2196032T3 (es) | 2003-12-16 |
| US5952002A (en) | 1999-09-14 |
| EP1253201A2 (en) | 2002-10-30 |
| AU7547394A (en) | 1995-02-20 |
| ATE236980T1 (de) | 2003-04-15 |
| CA2167578C (en) | 2007-03-13 |
| DE69432486D1 (de) | 2003-05-15 |
| EP0784685B1 (en) | 2003-04-09 |
| EP1253201A3 (en) | 2003-01-08 |
| DE69432486T2 (de) | 2004-03-04 |
| ZA945275B (en) | 1995-02-24 |
| ATE401406T1 (de) | 2008-08-15 |
| JP3829290B2 (ja) | 2006-10-04 |
| WO1995003414A2 (en) | 1995-02-02 |
| EP0784685A2 (en) | 1997-07-23 |
| DE69435117D1 (de) | 2008-08-28 |
| JPH09504941A (ja) | 1997-05-20 |
| PT1253201E (pt) | 2008-10-27 |
| ES2311005T3 (es) | 2009-02-01 |
| PT784685E (pt) | 2003-08-29 |
| EP1253201B1 (en) | 2008-07-16 |
| DK0784685T3 (da) | 2003-08-04 |
| WO1995003414A3 (en) | 1995-03-02 |
| CA2167578A1 (en) | 1995-02-02 |
| DK1253201T3 (da) | 2008-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Koch et al. | Immunogenic and protective properties of chicken anaemia virus proteins expressed by baculovirus | |
| M. Noteborn et al. | Chicken anaemia virus infection: molecular basis of pathogenicity | |
| NL9301272A (nl) | Chicken Anemia Virus mutanten en vaccins en toepassingen gebaseerd op de virale eiwitten VP1, VP2 en VP3 of daarvoor coderende sequenties van dat virus. | |
| CA2620727C (en) | Porcine circovirus type 2 vaccine | |
| AU696656B2 (en) | Chimeric infectious bursal disease virus cDNA clones, expression products and vaccines based thereon | |
| DE69222840T2 (de) | Verfahren zur herstellung von untereinheisimpfstoff gegen schweinparvovirus | |
| JP2846116B2 (ja) | 鶏貧血症ウィルス（ｃａｖ）組換え核酸、それを含むｃａｖ診断キット、および原核細胞または真核細胞、ならびにｃａｖ２本鎖ｄｎａの製造方法 | |
| US6071520A (en) | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor | |
| AU720640B2 (en) | Neutralizing conformational epitopes of chicken anemia virus | |
| US7427658B2 (en) | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor | |
| KR19990028766A (ko) | 마렉 질환 바이러스 유전자 및 마렉 질환 예방용 백신에서그의 용도 | |
| Coombes et al. | Chicken anaemia virus: a short review | |
| US6217870B1 (en) | Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1 VP2 and VP3 or sequences of that virus coding therefor | |
| JP2905483B2 (ja) | 弱毒化ヘルペスウイルス及び外来性ｄｎａを含むヘルペスウイルス | |
| JPH10501964A (ja) | 試験管内でｇＤ遺伝子を発現するように形質転換されたヘルペスウィルス | |
| CN114107227B (zh) | 一种同时表达经典株和变异株传染性法氏囊病毒vp2蛋白的重组火鸡疱疹病毒活载体疫苗 | |
| US20060073160A1 (en) | Cloning of chicken anemia virus DNA | |
| US5993822A (en) | Modified live avian polymavirus vaccine in psittacine birds | |
| TWI361695B (en) | Pcv-2 vaccine | |
| Hulse | Recombinant DNA molecules and herpesviruses as potential immunogens against infectious bursal disease and Marek's disease viruses | |
| MXPA97009851A (en) | Epithopes conformational neutralizers of poem anemia viruses |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A1B | A search report has been drawn up | ||
| BV | The patent application has lapsed |