NL2033159B1 - Rapid seed propagation method of actinidia arguta - Google Patents

Rapid seed propagation method of actinidia arguta Download PDF

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Publication number
NL2033159B1
NL2033159B1 NL2033159A NL2033159A NL2033159B1 NL 2033159 B1 NL2033159 B1 NL 2033159B1 NL 2033159 A NL2033159 A NL 2033159A NL 2033159 A NL2033159 A NL 2033159A NL 2033159 B1 NL2033159 B1 NL 2033159B1
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seeds
actinidia arguta
sand
wet sand
sand storage
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NL2033159A
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Dutch (nl)
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Cheng Beibei
Bai Bing
Zhang Guojun
Zhang Jijun
Wang Dongsheng
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Univ Hebei Normal Sci & Tech
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention provides a rapid seed propagation method of Actinidia arguta, including the following steps: collecting Actinidia arguta seeds, performing seed soaking, sand storage and pregermination, and sowing the seeds; the sand storage includes 5 mixing the Actinidia arguta seeds with wet sand, and placing the mixture at 2—4°C for 80—90 d; the pregermination includes applying a layer of wet sand on seeds after sand storage, and applying a thin film for pregermination until cracks appear. According to the present invention, the seed dormancy of Actinidia arguta is broken 10 and the germination time is greatly shortened through seed soaking, sand storage and pregermination. Seeds harvested in autumn are generally sown in April of the following year, while the seeds of the present invention can be sown in January of the following year, thereby improving the seed propagation efficiency 15 of Actinidia arguta.

Description

RAPID SEED PROPAGATION METHCD OF ACTINIDIA ARGUTA
TECHNICAL FIELD
The present invention belongs to the technical field of crop cultivation, and particularly relates to a rapid seed propagation method of Actinidia arguta.
BACKGROUND ART
Actinidia arguta is a kind of perennial deciduous vine plant under Actinidiaceae of Actinidia Lindl, prefers coolness and is distributed very widely. With smooth and hairless skins and green- yellow or green fleshes, the Actinidia arguta fruits are sweet and sour, full of juice and rich in vitamins, amino acids and other nutrients. The fruits can be eaten fresh or processed into fruit
Juice and fruit wine, and the economic value is high.
At present, Actinidia arguta is mostly propagated from cuttage and grafting, but seeding is still an indispensable link in the seed selection and crossbreeding works of new varieties.
However, there are still some difficulties in sowing and seedling of Actinidia arguta. Firstly, Actinidia arguta seeds are relatively small, with a thousand seed weight of only about 1.5 g.
Secondly, in northern China, the interval from acquisition of
Actinidia arguta seeds to seed germination in the following year for sowing may be up to 6 months, which is a long time and seriously restricting the progress of follow-up works.
SUMMARY
In view of this, the present invention is intended to provide a rapid seed propagation method of Actinidia arguta, which breaks seed dormancy, promotes early seed germination, and shortens the time for seeding propagation.
In order to achieve the above-mentioned purpose, the present invention provides the following technical solutions:
A rapid seed propagation method of Actinidia arguta includes the following steps: collecting Actinidia arguta seeds, performing seed soaking, sand storage and pregermination and sowing; the sand storage includes mixing the Actinidia arguta seeds with wet sand, and placing the mixture at 2-4°C for 80-90 d; the pregermination includes applying a layer of wet sand on seeds after sand storage, and applying thin films for pregermination until cracks appear.
Preferably, the Actinidia arguta seeds are collected from mature fruits of Actinidia arguta, and the fully mature fruits are put in a mesh bag with a diameter of 1-2 mm, kneaded and washed to remove impurities to obtain the seeds.
More preferably, the obtained seeds are dried in shade for 3- 5d.
Preferably, the seed soaking includes soaking seeds in water for 24 h.
Preferably, during the sand storage or pregermination, a moisture content of the wet sand is 55-65%.
Preferably, during the sand storage, the seeds are mixed with wet sand at a volume ratio of 1:5-10.
More preferably, during the sand storage, a thickness of the wet sand at the bottom of the seeds is more than 3 cm, and a thickness of the wet sand at the top is more than 1 cm.
More preferably, during the sand storage, the seeds are turned over every 20-30 d, and water is replenished to maintain the moisture content of the wet sand.
Preferably, during the pregermination, a thickness of the wet sand at the bottom of the seeds is more than 5 cm, and a thickness of the wet sand at the top is more than 0.5 cm.
Preferably, the sowing includes sowing seeds in a greenhouse and applying films.
Compared with the prior art, the present invention has the following advantages:
The present invention provides a rapid seed propagation method of Actinidia arguta. The method is relatively simple and effective, can greatly save time compared with the traditional sowing propagation of Actinidia arguta, and is beneficial to break the seed dormancy and shorten the germination time.
At present, Actinidia arguta seeds are generally collected in
October and sown in April of the following year; in the present invention, Actinidia arguta seeds can be sown in January of the following year through seed soaking, sand storage and pregermination, and the Actinidia arguta plants can reach a height of more than 25 cm in April - May, making its propagation efficiency significantly better than the prior art.
Moreover, the present invention is of important significance to enrichment of the Actinidia arguta seedling cultivation process, and especially to the rapid propagation and promotion of excellent family and provenance of Actinidia arguta.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1: Actinidia arguta seedling emergence in a rapid seed propagation method of example 1 of the present invention on
January 25.
FIG. 2: Actinidia arguta seedling growth in a rapid seed propagation method of example 1 of the present invention on May 12.
DETAILED DESCRIPTION OF THE EMBODIMENTS
The present invention provides a rapid seed propagation method of Actinidia arguta, which can effectively break the seed dormancy of Actinidia arguta and shorten the germination time. The seed propagation of the present invention includes the following steps: Actinidia arguta seeds are collected, and seed soaking, sand storage, pregermination and sowing are performed; the sand storage includes mixing the Actinidia arguta seeds with wet sand, and placing the mixture at 2-4°C for 80-90 d; the pregermination includes applying a layer of wet sand on seeds after sand storage, and applying thin films for pregermination until cracks appear.
In the present invention, preferably, the Actinidia arguta seeds are collected from mature fruits of Actinidia arguta, and the fully mature fruits are put in a mesh bag with a diameter of 1-2 mm, kneaded and washed to remove impurities to obtain seeds; further preferably, the Actinidia arguta seeds are collected in early October.
In the present invention, preferably, the seeds are dried in shade after the seeds are collected; further preferably, the time of the drying in shade is 3-5 d; more preferably, the time is 4 d.
In the present invention, preferably, the seed scaking includes soaking seeds in water for 18-36 h; further preferably, the time of the seed soaking is 24 h. The seed scaking allows the seeds to absorb sufficient water, and avoid the problem of loss of vitality of the seeds due to water consumption during sand storage.
In the present invention, preferably, during sand storage or pregermination, a moisture content of the wet sand is 55-65%; further preferably, a moisture content is 58-62%; more preferably, a moisture content is 60%. From a sensory point, the wet sand is appropriate in a state of being gathered when squeezed by hand and being dispersed when released by hand.
In the present invention, preferably during sand storage, the seeds are mixed with the wet sand at a volume ratio of 1:5-10; further preferably, the seeds are mixed with the wet sand at a volume ratio of 1:6-9; more preferably, the seeds are mixed with the wet sand at a volume ratio of 1:7. Preferably, during sand storage, a thickness of the wet sand at the bottom of the seeds is more than 3 cm, and a thickness of the wet sand at the top is more than 1 cm. As an implementable mode, a square container is selected, the seeds are mixed with the wet sand at a volume ratio of 1:7, a 3 cm-thick layer of wet sand is spread at the bottom, then seeds are placed and a 1 cm-chick layer of wet sand is applied on the top, the container is covered with a lid and stored at 2-4°C for sand storage.
In the present invention, preferably, the days of the sand storage is 82-88 d; more preferably, the days of the sand storage is 85 d. Further preferably, during sand storage, the seeds are turned over every 20-30 d, and water is replenished to maintain the moisture content of the wet sand; more preferably, the seeds are turned over every 25 d.
In the present invention, preferably, during pregermination, a thickness of the wet sand at the bottom of the seeds is more than 5 cm, and a thickness of the wet sand at the top is more than 0.5 cm; further preferably, during pregermination, water is sprayed once a day to maintain the moisture content of the wet sand. As an implementable mode, ceramic pots are used as pregermination containers, a 5 cm-thick layer of wet sand is spread at the bottom, then seeds are placed, a 0.5 cm-thick layer of wet sand is applied on the top, the pots are covered with 5 plastic films for heat and moisture preservation, water is sprayed once a day, and finally the pots are placed in a solar greenhouse.
In the present invention, pregermination takes about 10 d, and cracks appear in most of the Actinidia arguta seeds.
In the present invention, preferably, the sowing includes: sowing seeds in a greenhouse and applying the films; further preferably, Actinidia arguta seeds and the fine sand in pregermination are evenly broadcast and covered with films; more preferably, during broadcasting, a 0.5 cm-thick layer of fine soil is applied above the seeds. As an implementable mode, the land is prepared into a field and watered thoroughly in the greenhouse, and the Actinidia arguta seeds and the fine sand from the pots for pregermination are distributed evenly and thinly on the field, and the fine soil is sieved through a sieve with a diameter of 0.5 cm and applied on the seeds thinly (a thickness of 0.5 cm), and films are applied; after most of the seeds break through the soil and germinate, the films are removed, and shade nets.
The technical solutions provided by the present invention will be explained below in detail in combination with the embodiments, but these embodiments should not be understood as a limitation to the protection scope of the present invention.
Example 1
A rapid seed propagation method of Actinidia arguta included the following steps: (1) Seed collection: Actinidia arguta fruits from Zushan
Mountain were collected on October 2, 2020. (2) Seed cleaning: on October 5, 2020, Actinidia arguta fruits were put in a mesh bag with a diameter of 1-2 mm, fleshes were gently kneaded by hand, and seeds were cleaned to remove impurities. (3) Drying in shade: from October 6, 2020 to October 10, 2020, Actinidia arguta seeds were dried in shade indoors and protected from sun exposure.
(4) Seed soaking: on October 10, 2020, the seeds dried in shade were soaked in water for 24 h. (5) Sand storage: Actinidia arguta seeds were divided into 2 parts, and a 3 cm-thick layer of fine sand was placed in a 20 cmx10 cmx6 cm (L x W x H) crisper on October 11, 2020, then the
Actinidia arguta seeds and wet sand were spread evenly at a ratio of 1:7, the wet sand was appropriate in a state of being gathered when squeezed by hand and being dispersed when released by hand, a 1 cm-thick layer of wet sand was placed on the top, and a lid was put on the plastic crisper; finally, the plastic crisper was put in a refrigerator at 2-4°C and subjected to sand storage for 80 d, the seeds were turned over every 20 d, water was replenished, and then the seeds were removed on December 30, 2020. (6) Pregermination: from December 31, 2020 to January 2, 2021, ceramic pots with a height of 7-8 cm were chosen, a 5 cm- thick layer of fine sand was spread, the Actinidia arguta seeds stored in sand in the refrigerator and the wet sand were distributed as thinly as possible on the fine sand in the pots; then a 0.5 cm-thick layer of fine sand was placed on the top, wa- ter was sprayed, and plastic films were applied for heat and moisture preservation; water was sprayed once a day, and finally the pots were placed in a solar greenhouse. (7) Sowing: on January 12, 2021, most of the Actinidia arguta seeds were cracked. The land was prepared into a field and watered thoroughly in the greenhouse, and the Actinidia arguta seeds from the pots and the fine sand were distributed evenly and thinly on the field, and the fine soil was sieved through a sieve with a diameter of 0.5 cm and applied on the seeds thinly (a thickness of 0.5 cm), and finally films were applied; after most of the seeds broke through the soil and germinated, the films were removed, and shade nets were applied on January 20, 2021.
Example 2
A rapid seed propagation method of Actinidia arguta included the following steps: (1) Seed collection: Actinidia arguta fruits were collected in early October. (2) Seed cleaning: Actinidia arguta fruits were put in a mesh bag with a diameter of 1-2 mm, fleshes were gently kneaded by hand, and seeds were cleaned to remove impurities. (3) Drying in shade: Actinidia arguta seeds were dried in shade indoors for 3 d and protected from sun exposure. (4) Seed soaking: seeds dried in shade were soaked in water for 24 h. (5) Sand storage: a 3 cm-thick layer of fine sand was placed in a 20 cmx10 cmx6 cm (L x W x H) crisper, then the Actinidia arguta seeds and wet sand were spread evenly at a ratio of 1:5, the wet sand was appropriate in a state of being gathered when squeezed by hand and being dispersed when released by hand, a 1 cm-thick layer of wet sand was placed on the top, and a lid was put on the plastic crisper; finally, the plastic crisper was put in a refrigerator at 2-4°C and subjected to sand storage for 85 d, the seeds were turned over every 25 d, and water was replenished. (6) Pregermination: the seeds were taken cut after sand storage; ceramic pots with a height of 7-8 cm were chosen, a 5 cm- thick layer of fine sand was spread, the Actinidia arguta seeds stored in sand in the refrigerator and the wet sand were distributed as thinly as possible on the fine sand in the pots; then a 0.5 cm-thick layer of fine sand was placed on the top, water was sprayed, and plastic films were applied for heat and moisture preservation ; water was sprayed once a day, and finally the pots were placed in a solar greenhouse. {7) Sowing: around 10 d, most of the Actinidia arguta seeds were cracked. The land was prepared into a field and watered thoroughly in the greenhouse, and the Actinidia arguta seeds from the pots and the fine sand were distributed evenly and thinly on the field, and the fine soil was sieved through a sieve with a diameter of 0.5 cm and applied on the seeds thinly (a thickness of 0.5 cm), and finnaly films were applied; after most of the seeds broke through the soil and germinated, the films were removed, and shade nets were applied.
Example 3
A rapid seed propagation method of Actinidia arguta included the following steps: (1) Seed collection: Actinidia arguta fruits were collected in early October. (2) Seed cleaning: Actinidia arguta fruits were put in a mesh bag with a diameter of 1-2 mm, fleshes were gently kneaded by hand, and seeds were cleaned to remove impurities. (3) Drying in shade: Actinidia arguta seeds were dried in shade indoors for 5 d and protected from sun exposure. (4) Seed soaking: seeds dried in shade were soaked in water for 24 h. (5) Sand storage: a 3 cm-thick layer of fine sand was placed in a 20 cmx10 cmx6 cm (L x W x H) crisper, then the Actinidia arguta seeds and wet sand were spread evenly at a ratio of 1:10, the wet sand was appropriate in a state of being gathered when squeezed by hand and being dispersed when released by hand, a 1 cm-thick layer of wet sand was placed on the top, and a lid was put on the plastic crisper; finally, the plastic crisper was put in a refrigerator at 2-4°C and subjected to sand storage for 90 d, seeds were turned over every 30 d, and water was replenished. (6) Pregermination: the seeds were taken out after sand storage; ceramic pots with a height of 7-8 cm were chosen, and a 5 cm-thick layer of fine sand was spread, the Actinidia arguta seeds stored in sand in the refrigerator and the wet sand were distributed as thinly as possible on the fine sand in the pots; then a 0.5 cm-thick layer of fine sand was placed on the top, wa- ter was sprayed, and plastic films were applied for heat and moisture preservation; water was sprayed once a day, and finally the pots were placed in a solar greenhouse. (7) Sowing: around 10 d, most of the Actinidia arguta seeds were cracked. The land was prepared into a field and watered thoroughly in the greenhouse, and the Actinidia arguta seeds from the pots and the fine sand were distributed evenly and thinly on the field, and the fine soil was sieved through a sieve with a diameter of 0.5 cm and applied on the seeds thinly (a thickness of 0.5 cm), and finally films were applied; after most of the seeds broke through the soil and germinated, the films were removed, and shade nets were applied.
The above-mentioned contents are only the preferred embodiments of the present invention. It should be noted that some improvements and modifications may be made by those of ordinary skill in the art without deviating from the principle of the present invention, and these improvements and modifications should be also regarded to fall into the protection scope of the present invention.

Claims (1)

CONCLUSIESCONCLUSIONS 1. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta, die de volgende stappen omvat: het verzamelen van Actinidia arguta-zaden en het uitvoeren van zaadweek, zandopslag en voorkieming en zaaien; waarbij de zandopslag omvat het mengen van de Actinidia arguta- zaden met nat zand, en het plaatsen van het mengsel op 2 tot 4 °C gedurende 80 tot 90 dagen; waarbij de voorkieming omvat het aanbrengen van een laag nat zand op zaden na zandopslag, en het aanbrengen van dunne films voor voorkieming totdat er barsten ontstaan.1. Method of rapid seed multiplication of Actinidia arguta, which includes the following steps: collecting Actinidia arguta seeds and carrying out seed soaking, sand storage and pre-germination and sowing; wherein the sand storage involves mixing the Actinidia arguta seeds with wet sand, and placing the mixture at 2 to 4°C for 80 to 90 days; where pre-germination involves applying a layer of wet sand to seeds after sand storage, and applying thin films for pre-germination until cracks develop. 2. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1, waarbij de Actinidia arguta-zaden worden verzameld uit rijpe vruchten van Actinidia arguta, en de volledig rijpe vruchten in een netzak met een diameter van 1 tot 2 mm worden gedaan, gekneed en gewassen om onzuiverheden te verwijderen om de zaden te verkrijgen.A method for rapid seed multiplication of Actinidia arguta according to claim 1, wherein the Actinidia arguta seeds are collected from ripe fruits of Actinidia arguta, and the fully ripe fruits are placed in a mesh bag of 1 to 2 mm in diameter, kneaded and washed to remove impurities to obtain the seeds. 3. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 2, waarbij de verkregen zaden 3 tot 5 dagen in de schaduw worden gedroogd.A method for rapid seed propagation of Actinidia arguta according to claim 2, wherein the obtained seeds are dried in the shade for 3 to 5 days. 4. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1, waarbij het weken van de zaden het weken van zaden in water gedurende 24 uur omvat.The method for rapid seed multiplication of Actinidia arguta according to claim 1, wherein soaking the seeds includes soaking seeds in water for 24 hours. 5. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1, waarbij tijdens de zandopslag of voorkieming, een vochtgehalte van het natte zand 55 tot 65% is.A method for rapid seed multiplication of Actinidia arguta according to claim 1, wherein during sand storage or pre-germination, a moisture content of the wet sand is 55 to 65%. 6. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1, waarbij tijdens de zandopslag, de zaden worden gemengd met nat zand in een volumeverhouding van 1:(5 totA method for rapid seed multiplication of Actinidia arguta according to claim 1, wherein during sand storage, the seeds are mixed with wet sand in a volume ratio of 1:(5 to 10).10). 7. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1 of 6, waarbij tijdens de zandopslag, een dikte van het natte zand aan de onderkant van de zaden meer dan 3 cm is, en een dikte van het natte zand aan de bovenkant meer dan 1 cm is.A method for rapid seed multiplication of Actinidia arguta according to claim 1 or 6, wherein during sand storage, a thickness of the wet sand at the bottom of the seeds is more than 3 cm, and a thickness of the wet sand at the top is more than 1 cm. 8. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1 of 6, waarbij tijdens de zandopslag, de zaden elke 20 tot 30 dagen worden omgedraaid, en water wordt aangevuld om het vochtgehalte van het natte zand te handhaven.A method for rapid seed multiplication of Actinidia arguta according to claim 1 or 6, wherein during sand storage, the seeds are turned every 20 to 30 days, and water is replenished to maintain the moisture content of the wet sand. 9. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1, waarbij tijdens de voorkieming, een dikte van het natte zand aan de onderkant van de zaden meer dan 5 cm is, en een dikte van het natte zand aan de bovenkant meer dan 0,5 cm is.The method for rapid seed multiplication of Actinidia arguta according to claim 1, wherein during pre-germination, a thickness of the wet sand at the bottom of the seeds is more than 5 cm, and a thickness of the wet sand at the top is more than 0. 5 cm. 10. Werkwijze voor snelle zaadvermeerdering van Actinidia arguta volgens conclusie 1, waarbij het zaaien het zaaien van zaden in een kas en het aanbrengen van films omvat.A method for rapid seed propagation of Actinidia arguta according to claim 1, wherein the sowing comprises sowing seeds in a greenhouse and applying films.
NL2033159A 2022-07-30 2022-09-27 Rapid seed propagation method of actinidia arguta NL2033159B1 (en)

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CN104429764A (en) * 2014-11-07 2015-03-25 重庆茂升林业开发有限公司 Kiwi fruit planting method
CN106258773A (en) * 2016-10-14 2017-01-04 苏州名夫果园生态农业有限公司 A kind of cultural method of red kiwifruit
CN108781965A (en) * 2018-05-24 2018-11-13 道真自治县精品水果科技开发有限公司 Kiwi berry method for culturing seedlings

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