NL2032828B1 - Microbial inoculant for composting livestock and poultry manure and preparation method thereof - Google Patents

Microbial inoculant for composting livestock and poultry manure and preparation method thereof Download PDF

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Publication number
NL2032828B1
NL2032828B1 NL2032828A NL2032828A NL2032828B1 NL 2032828 B1 NL2032828 B1 NL 2032828B1 NL 2032828 A NL2032828 A NL 2032828A NL 2032828 A NL2032828 A NL 2032828A NL 2032828 B1 NL2032828 B1 NL 2032828B1
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parts
bacteria
preparation
microbial inoculant
trichoderma
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NL2032828A
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English (en)
Inventor
Dong Wenyi
Zhao Jianzhong
Zhang Yufeng
Yang Yan
Jiang Lihua
Liu Yansheng
Hu Bin
Kong Fanmei
Zhang Xiaocun
Wang Xiangwen
Liu Enke
Original Assignee
Univ Shandong Agricultural
Inst Agricultural Resources & Environment Shandong Academy Agricultural Sciences
Qingdao Xingkai Environmental Tech Co Ltd
Inst Of Environment And Sustainable Development In Agriculture Chinese Academy Of Agricultural Scien
Shandong Agricultural Tech Promotion Center Shandong Agricultural And Rural Development Research Cen
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Priority to NL2032828A priority Critical patent/NL2032828B1/en
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Publication of NL2032828B1 publication Critical patent/NL2032828B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (1)

  1. CONCLUSIES
    1. Microbieel inoculant voor het composteren van mest van vee en pluimvee, met het kenmerk, dat het wordt bereid uit de volgende bestanddelen in massadelen: 2,0 tot 3,0 delen bacillus subtilis, 2,6 tot 3,7 delen azotobacter vinelandii, 1,8 tot 2,4 delen paeci- lomyces lilacinus, 3,3 tot 4,0 delen bacillus coagulans, 2,1 tot 3,1 delen trichoderma harzianum, 4,0 tot 4,2 delen streptomyces microflavus, 0,6 tot 0,8 delen trichoderma, 0,3 tot 0,4 delen or- ganofosforbacteriën, 0,3 tot 0,4 delen kaliumbacterién en 0,5 tot 1,0 onderdeel van rhizobium astragali.
    2. Microbieel inoculant voor het composteren van mest van vee en pluimvee volgens conclusie 1, met het kenmerk, dat de streptomyces microflavus hoofdzakelijk wordt gefermenteerd uit een vloeibaar kweekmedium van sojabonenkoekpoeder, glucose, een calciumhydro- chloride en een natriumchloride.
    3. Microbieel inoculant voor het composteren van mest van vee en pluimvee volgens conclusie 1 of 2, met het kenmerk, dat een berei- dingswijze de volgende stappen omvat:
    S1. stamcultuur: het enten van de bacillus subtilis, azotobacter vinelandii, paecilomyces lilacinus, bacillus coagulans, trichoder- ma harzianum, streptomyces microflavus, trichoderma, organofosfor bacteriën, kaliumbacteriën en rhizobium astragali respectievelijk in elk kweekmedium voor het kweken;
    S2. bereiding van bacteriële oplossing: het plaatsen van de ver- schillende bacteriestammen die in stap S1 zijn gekweekt in een me- dicijnfles van een zaadkweekmedium in een modus van inoculatie met schuin oppervlak, en het gebruiken van een vloeibaar kweekmedium voor afzonderlijk kweken, om een bacteriële oplossing van de bac- teriestam te verkrijgen;
    S3. menging van bacteriële oplossing: alle typen van de in stap S2 bereide bacteriële oplossing in een met zaden beladen fermentatie- tank doen, roeren en mengen, om een samengestelde zaadoplossing te verkrijgen;
    S4. bereiding van samengestelde bacteriële oplossing: het enten van alle zaadoplossing in stap S3 in een zaadtank die is beladen met een fermentatie kweekmedium voor fermentatie en kweken, om een samengestelde bacteriële oplossing te verkrijgen;
    85. bereiding van microbieel inoculant: het drogen en poeder- sproeien van de samengestelde bacteriële oplossing in stap S4, om een microbieel inoculant als eindproduct te verkrijgen; en
    S6. verpakking en opslag: het microbiële inoculant van het eind- product in de stap S5 toevoegen aan een vulmachine, het in een container verpakken met behulp van de vulmachine en het in een op- slag plaatsen.
NL2032828A 2022-08-23 2022-08-23 Microbial inoculant for composting livestock and poultry manure and preparation method thereof NL2032828B1 (en)

Priority Applications (1)

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NL2032828A NL2032828B1 (en) 2022-08-23 2022-08-23 Microbial inoculant for composting livestock and poultry manure and preparation method thereof

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NL2032828A NL2032828B1 (en) 2022-08-23 2022-08-23 Microbial inoculant for composting livestock and poultry manure and preparation method thereof

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NL2032828B1 true NL2032828B1 (en) 2024-03-04

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1137505A (zh) * 1995-06-05 1996-12-11 孙克用 多功能全价强力有机复合肥及其制法
WO2017184810A1 (en) * 2016-04-21 2017-10-26 Koch Agronomic Services, Llc Microbial inoculant compositions and uses thereof
CN108484267A (zh) * 2018-05-04 2018-09-04 东北农业大学 一种复合微生物生态有机肥及其制备方法和应用
CN111646837A (zh) * 2020-04-30 2020-09-11 湖北绿道农业发展有限公司 一种蔬菜生物有机肥、其制备方法及其施肥方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1137505A (zh) * 1995-06-05 1996-12-11 孙克用 多功能全价强力有机复合肥及其制法
WO2017184810A1 (en) * 2016-04-21 2017-10-26 Koch Agronomic Services, Llc Microbial inoculant compositions and uses thereof
CN108484267A (zh) * 2018-05-04 2018-09-04 东北农业大学 一种复合微生物生态有机肥及其制备方法和应用
CN111646837A (zh) * 2020-04-30 2020-09-11 湖北绿道农业发展有限公司 一种蔬菜生物有机肥、其制备方法及其施肥方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GREFF BABETT ET AL: "Influence of microbial inoculants on co-composting of lignocellulosic crop residues with farm animal manure: A review", JOURNAL OF ENVIRONMENTAL MANAGEMENT, vol. 302, 16 November 2021 (2021-11-16), AMSTERDAM, NL, pages 114088, XP093032716, ISSN: 0301-4797, DOI: 10.1016/j.jenvman.2021.114088 *
ZHOU CHENG ET AL: "A new strategy for co-composting dairy manure with rice straw: Addition of different inocula at three stages of composting", WASTE MANAGEMENT., vol. 40, 30 March 2015 (2015-03-30), US, pages 38 - 43, XP093032768, ISSN: 0956-053X, DOI: 10.1016/j.wasman.2015.03.016 *

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