MXPA06014461A - Electroporador que tiene un miembro hueco alargado. - Google Patents

Electroporador que tiene un miembro hueco alargado.

Info

Publication number
MXPA06014461A
MXPA06014461A MXPA06014461A MXPA06014461A MXPA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A MX PA06014461 A MXPA06014461 A MX PA06014461A
Authority
MX
Mexico
Prior art keywords
hollow member
elongated hollow
electroporation
electroporator
elongated
Prior art date
Application number
MXPA06014461A
Other languages
English (en)
Inventor
-Keun Jun Chan
Keun-Chang Cho
Chan-Ii Chung
Young-Shik Shin
Jeong-Ah Kim
Neon-Cheol Jung
Original Assignee
Digital Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Digital Biotechnology Co Ltd filed Critical Digital Biotechnology Co Ltd
Publication of MXPA06014461A publication Critical patent/MXPA06014461A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un aparato de electroporacion que contiene miembro hueco alargado para poder proporcionar un campo electrico uniforme durante la electroporacion, donde especificamente, la electroporacion se lleva a cabo al aplicar impulsos electricos a traves de un par de electrodos desde ambas partes extremas del miembro hueco alargado, despues de que el miembro hueco se carga con el especimen de fluido que incluye celulas y material que puede inyectarse en las celulas.
MXPA06014461A 2004-06-12 2005-06-13 Electroporador que tiene un miembro hueco alargado. MXPA06014461A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20040043312 2004-06-12
KR20040088245 2004-11-02
PCT/KR2005/001792 WO2006001614A1 (en) 2004-06-12 2005-06-13 Electroporator having an elongated hollow member

Publications (1)

Publication Number Publication Date
MXPA06014461A true MXPA06014461A (es) 2007-05-23

Family

ID=35781995

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA06014461A MXPA06014461A (es) 2004-06-12 2005-06-13 Electroporador que tiene un miembro hueco alargado.

Country Status (13)

Country Link
US (4) US8932850B2 (es)
EP (1) EP1766004B1 (es)
JP (2) JP4519915B2 (es)
KR (1) KR100853102B1 (es)
CN (1) CN103060191B (es)
AU (1) AU2005257583A1 (es)
BR (1) BRPI0512002A (es)
CA (1) CA2570557C (es)
IL (1) IL179384A0 (es)
MX (1) MXPA06014461A (es)
NO (1) NO20070170L (es)
RU (1) RU2006143560A (es)
WO (1) WO2006001614A1 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
AU2005257583A1 (en) 2004-06-12 2006-01-05 Digital Bio Technology Co., Ltd. Electroporator having an elongated hollow member
KR101084528B1 (ko) 2008-04-15 2011-11-18 인비트로겐 싱가포르 피티이. 엘티디. 전기천공 장치용 파이펫 팁
US9017991B2 (en) * 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
CN105396220A (zh) * 2010-09-29 2016-03-16 哈佛学院院长等 用纳米线的分子递送
KR101492598B1 (ko) 2010-10-18 2015-02-16 라이프 테크놀로지스 홀딩스 프리베이트 리미티드 전기천공 장치용 파이펫 팁
US9382510B2 (en) 2011-08-25 2016-07-05 Jian Chen Methods and devices for electroporation
JP6269968B2 (ja) 2012-12-12 2018-01-31 国立大学法人豊橋技術科学大学 外来物質導入装置および外来物質導入細胞の製造方法
KR101492599B1 (ko) 2014-05-07 2015-02-16 라이프 테크놀로지스 홀딩스 프리베이트 리미티드 전기천공 장치용 파이펫 팁
WO2016003485A1 (en) 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
WO2016112315A2 (en) 2015-01-09 2016-07-14 President And Fellows Of Harvard College Nanowire arrays for neurotechnology and other applications
ES2880473T5 (es) 2015-01-30 2024-05-09 Univ California Suministro de proteínas en células hematopoyéticas primarias
US20190136224A1 (en) * 2016-05-31 2019-05-09 Massachusetts Institute Of Technology Hydrodynamically Controlled Electric Fields for High Throughput Transformation & High Throughput Parallel Transformation Platform
CN107058095B (zh) * 2017-02-09 2020-07-28 清华大学 手持式电穿孔装置
HRP20220615T1 (hr) 2017-06-30 2022-06-24 Inscripta, Inc. Postupci, moduli, instrumenti i sustavi za automatiziranu obradu stanica
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
EP3687621A4 (en) * 2017-09-30 2021-08-11 Inscripta, Inc. CONTINUOUS FLOW ELECTROPORATION INSTRUMENTATION
JP2019118291A (ja) * 2017-12-28 2019-07-22 株式会社ベックス エレクトロポレーション用チャンバー及びチャンバーホルダー
EP3775159A4 (en) 2018-03-29 2022-01-19 Inscripta, Inc. AUTOMATED REGULATION OF CELL GROWTH RATES FOR INDUCTION AND TRANSFORMATION
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
EP4070802A1 (en) 2018-06-30 2022-10-12 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
JP7393011B2 (ja) * 2018-12-20 2023-12-06 国立大学法人豊橋技術科学大学 電気穿孔装置及び外来物質導入細胞の製造方法
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
US10920189B2 (en) 2019-06-21 2021-02-16 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
KR20220133257A (ko) 2020-01-27 2022-10-04 인스크립타 인코포레이티드 전기천공 모듈 및 기구
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CN113322181A (zh) * 2021-06-10 2021-08-31 上海大学 一种细胞裂解装置及基于细胞裂解装置的细胞纯化和/或细胞裂解的方法
EP4279085A1 (en) 2022-05-20 2023-11-22 Mnemo Therapeutics Compositions and methods for treating a refractory or relapsed cancer or a chronic infectious disease
WO2024059618A2 (en) 2022-09-13 2024-03-21 Arsenal Biosciences, Inc. Immune cells having co-expressed tgfbr shrnas
WO2024059824A2 (en) 2022-09-16 2024-03-21 Arsenal Biosciences, Inc. Immune cells with combination gene perturbations

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3017399A (en) 1958-05-16 1962-01-16 Escambia Chem Corp Production of vinyl chloride polymers
US4099548A (en) 1976-08-25 1978-07-11 Oxford Laboratories Inc. Hand-held pipette for repetitively dispensing precise volumes of liquid
JPS6349070A (ja) * 1986-08-19 1988-03-01 Shimadzu Corp 送液型細胞融合チヤンバ
JPS63160574A (ja) * 1986-12-24 1988-07-04 Norin Suisan Gijutsu Joho Kyokai 微粒子制御装置
GB8807271D0 (en) 1988-03-26 1988-04-27 Preece A W Cell fusion apparatus
US5007995A (en) * 1989-05-11 1991-04-16 Olympus Optical Co., Ltd. Device for electrofusion of cells
US6001617A (en) 1989-06-07 1999-12-14 Queen's University At Kingston Electroporation device and method of use
US5993434A (en) * 1993-04-01 1999-11-30 Genetronics, Inc. Method of treatment using electroporation mediated delivery of drugs and genes
JPH06349070A (ja) 1993-06-07 1994-12-22 Nec Corp 光ディスクの再生方法
US6074605A (en) 1995-03-10 2000-06-13 Entremed, Inc. Flow electroporation chamber and method
CN1195997A (zh) * 1995-03-10 1998-10-14 恩特雷麦德有限公司 流式电穿孔室和方法
DE19725190A1 (de) 1997-06-14 1998-12-17 Innova Gmbh Vorrichtungen mit integrierten Elektroden aus elektrisch leitfähigen Kunststoffen
US6241701B1 (en) 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
SE9704076D0 (sv) * 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
US7456012B2 (en) * 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
US6293749B1 (en) 1997-11-21 2001-09-25 Asm America, Inc. Substrate transfer system for semiconductor processing equipment
US6287776B1 (en) 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
JPH11290058A (ja) 1998-04-10 1999-10-26 Unique Medical Imada:Kk 遺伝子導入用電極装置及び遺伝子導入装置
EP0961321B1 (en) 1998-05-29 2008-03-05 Kyocera Corporation High-frequency module
GB9812783D0 (en) 1998-06-12 1998-08-12 Cenes Ltd High throuoghput screen
WO2000034434A1 (en) 1998-12-07 2000-06-15 Acacia Biosciences, Inc. Multi-channel electrode arrays
US6627421B1 (en) 1999-04-13 2003-09-30 Imarx Therapeutics, Inc. Methods and systems for applying multi-mode energy to biological samples
GB9908681D0 (en) * 1999-04-16 1999-06-09 Central Research Lab Ltd Apparatus for, and method of, introducing a substance into an object
US6677114B1 (en) * 1999-04-20 2004-01-13 Target Discovery, Inc. Polypeptide fingerprinting methods and bioinformatics database system
WO2001014855A1 (en) 1999-08-20 2001-03-01 Charles William Robertson Liquid photometer using surface tension to contain sample
US20020090649A1 (en) 1999-12-15 2002-07-11 Tony Chan High density column and row addressable electrode arrays
US7270730B2 (en) 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
SE0003841D0 (sv) * 2000-10-20 2000-10-20 Daniel Chiu A method and apparatus for penetration of lipid bilayer membranes
US6818403B2 (en) * 2000-11-08 2004-11-16 Surface Logix, Inc. Method of monitoring haptotaxis
AU2002354569A1 (en) 2001-07-12 2003-01-29 Merck And Co., Inc. Electrical field stimulation of eukaryotic cells
US7018819B2 (en) * 2001-11-30 2006-03-28 Cellectricon Ab Method and apparatus for manipulation of cells and cell-like structures focused electric fields in microfludic systems and use thereof
AU2003206415A1 (en) 2002-01-07 2003-07-24 Uab Research Foundation Electroporation cuvette-pipette tips, multi-well cuvette arrays, and electrode template apparatus adapted for automation and uses thereof
DE10208188B4 (de) 2002-02-20 2006-05-24 Amaxa Gmbh Behälter mit zumindest einer Elektrode
US20040115784A1 (en) * 2002-09-30 2004-06-17 Maxcyte, Inc. Apparatus and method for streaming electroporation
US7384781B2 (en) 2003-04-30 2008-06-10 Moyle William R Sensors for biomolecular detection and cell classification
US20050118705A1 (en) * 2003-11-07 2005-06-02 Rabbitt Richard D. Electrical detectors for microanalysis
PL1577378T3 (pl) 2004-03-15 2010-02-26 Lonza Cologne Gmbh Zbiornik i urządzenie do wytwarzania pól elektrycznych w pojedynczych pomieszczeniach reakcyjnych
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
AU2005257583A1 (en) 2004-06-12 2006-01-05 Digital Bio Technology Co., Ltd. Electroporator having an elongated hollow member
JP4828933B2 (ja) 2005-12-22 2011-11-30 中央精機株式会社 微小物質移送方法
JP4721984B2 (ja) 2006-08-23 2011-07-13 カヤバ工業株式会社 鉄道車両用キャリパブレーキ装置
US7682256B2 (en) 2006-09-13 2010-03-23 Kop-Flex Inc. Flange design for filament wound composite shaft
SI22368A (sl) 2006-10-25 2008-04-30 Univerza V Ljubljani Fakulteta Za Elektrotehniko Konicasta komora z vgrajenimi elektrodami za elektroporacijo manjsega volumna, za elektrofuzijo in gensko transfekcijo
ES2534579T3 (es) 2007-02-23 2015-04-24 Lonza Cologne Gmbh Dispositivo y procedimiento para estabilizar el flujo a través de una cámara
KR101084528B1 (ko) 2008-04-15 2011-11-18 인비트로겐 싱가포르 피티이. 엘티디. 전기천공 장치용 파이펫 팁

Also Published As

Publication number Publication date
EP1766004A1 (en) 2007-03-28
NO20070170L (no) 2007-03-12
EP1766004B1 (en) 2016-08-31
US8932850B2 (en) 2015-01-13
IL179384A0 (en) 2007-03-08
CA2570557C (en) 2011-01-25
WO2006001614A1 (en) 2006-01-05
JP2008502356A (ja) 2008-01-31
US20190040350A1 (en) 2019-02-07
US20220154128A1 (en) 2022-05-19
BRPI0512002A (pt) 2008-01-22
EP1766004A4 (en) 2009-05-06
KR100853102B1 (ko) 2008-08-21
RU2006143560A (ru) 2008-07-20
AU2005257583A1 (en) 2006-01-05
KR20070064556A (ko) 2007-06-21
JP2010178746A (ja) 2010-08-19
US20150093813A1 (en) 2015-04-02
CN103060191B (zh) 2015-03-11
CN103060191A (zh) 2013-04-24
CA2570557A1 (en) 2006-01-05
US11130936B2 (en) 2021-09-28
US9890355B2 (en) 2018-02-13
US20070275454A1 (en) 2007-11-29
JP4519915B2 (ja) 2010-08-04

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