MX9606072A - Metodo para la deteccion de pseudomonas aeruginosa usando una reaccion de cadena de polimerasa. - Google Patents

Metodo para la deteccion de pseudomonas aeruginosa usando una reaccion de cadena de polimerasa.

Info

Publication number
MX9606072A
MX9606072A MX9606072A MX9606072A MX9606072A MX 9606072 A MX9606072 A MX 9606072A MX 9606072 A MX9606072 A MX 9606072A MX 9606072 A MX9606072 A MX 9606072A MX 9606072 A MX9606072 A MX 9606072A
Authority
MX
Mexico
Prior art keywords
oligonucleotide primer
sequence
detection
chain reaction
polymerase chain
Prior art date
Application number
MX9606072A
Other languages
English (en)
Other versions
MXPA96006072A (es
Inventor
Kevin Gary Korth
Sarah Elizabeth Heathcock
Linda Susan Huard
Original Assignee
Kimberly Clark Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/748,170 external-priority patent/US6048688A/en
Application filed by Kimberly Clark Co filed Critical Kimberly Clark Co
Publication of MXPA96006072A publication Critical patent/MXPA96006072A/es
Publication of MX9606072A publication Critical patent/MX9606072A/es

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

la presente invencion se refiere a un método para detectar la presencia de P. aeruginosa en una muestra de fluido. El método usa PCR para amplificar la expresion de un segmento de la secuencia de gen exotocin A. El método incluye el tratar capas de fragmentos de nucleotido con un primer imprimador de oligonucleotido y un segundo imprimador de oligonucleotido. Los imprimadores son suficientemente complementarios al fragmento para hibridizar una region teniendo de desde alrededor de 400 a 1200 pares de base. Deseablemente, el primer imprimador de oligonucleotido incluye la secuencia 5' - ACA ACG CCC TCA GCA TCA CCA - 3' y el segundo imprimador de oligonucleotido incluye la secuencia 5' - CGG GTC GAG CAG GCA CAA C Ñ- 3'.
MX9606072A 1995-12-15 1996-12-03 Metodo para la deteccion de pseudomonas aeruginosa usando una reaccion de cadena de polimerasa. MX9606072A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US869795P 1995-12-15 1995-12-15
US60/008,697 1995-12-15
US08748170 1996-11-12
US08/748,170 US6048688A (en) 1996-11-12 1996-11-12 Method for detection of Pseudomonas aeruginosa using polymerase chain reaction

Publications (2)

Publication Number Publication Date
MXPA96006072A MXPA96006072A (es) 1998-04-01
MX9606072A true MX9606072A (es) 1998-04-30

Family

ID=39427809

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9606072A MX9606072A (es) 1995-12-15 1996-12-03 Metodo para la deteccion de pseudomonas aeruginosa usando una reaccion de cadena de polimerasa.

Country Status (1)

Country Link
MX (1) MX9606072A (es)

Similar Documents

Publication Publication Date Title
KR0156290B1 (ko) 호열성 효소를 사용한 사슬 치환 증폭방법
DE69626111T2 (de) Universale primersequenz zur multiplex dna amplifikation
KR950704508A (ko) 비-포스포디에스테르 누클레오티드간 결합을 함유하는 합성 올리고누클레오티드의 서열화 방법(methods for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages)
AR085952A2 (es) Polinucleotidos cebadores
DE69729122D1 (de) KüNSTLICHE FEHLHYBRIDISIERUNG
SE9400522D0 (sv) Method and reagent for detecting specific nucleotide sequences
WO2002000928A3 (de) Diagnose von mit dem immunsystem assoziierten krankheiten durch bestimmung der cytosin-methylierung
AU2002360306A1 (en) Molecular detection systems utilizing reiterative oligonucleotide synthesis
ATE251674T1 (de) Verfahren, reagenz und salz zum nachweis und amplifikation von nukleinsäuresequenzen
KR930700680A (ko) Rna 레플리카제의 dna-의존성 rna 폴리머라제 활성을 이용한 핵산 증폭
EP0663447A3 (en) Method of detecting a specific polynucleotide
CA2239287A1 (en) A cascade nucleic acid amplification reaction
WO2001096612A3 (en) Method of identifying and quantifying specific fungi and bacteria
EP1212449A4 (en) AMPLIFICATION OF FLANKING SEQUENCES FROM UNKNOWN DNA
WO2003016565A3 (en) Dna sequence analysis
BR9808963A (pt) Taqman tm-pcr para a detecção de cepas de e. coli patogênicas
AU4391797A (en) The use of modular oligonucleotides as probes or primers in nucleic acid based assay
CA2318371A1 (en) Method for the detection of nucleic acid sequences
AU6975694A (en) Salmonella identification by the polymerase chain reaction
WO2001036669A3 (de) Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen
WO2002046454A3 (de) Diagnose von mit angiogenese assoziierten krankheiten durch bestimmung der methylierung von mit angiogenese assoziierten genen
WO2002006523A3 (en) Method for detecting pre-disposition to hepatotoxicity
DE69616102D1 (de) Amplikationsprimern und Nukleinsäuresonden zum Nachweis von Coccidioides immites
TW329476B (en) Method for detecting contaminant DNA in a protein product and kits used for the same purpose
KR930018037A (ko) 글루코세레브로시다제 유전자의 인트론 2중에서의 신규한 교셰(Gaucher)질환 돌연변이의 검출방법

Legal Events

Date Code Title Description
FG Grant or registration
MM Annulment or lapse due to non-payment of fees