MX9301521A - Fragmentos de acido nucleico y metodo para incrementar el contenido de lisina treonina de las semillas de las plantas. - Google Patents

Fragmentos de acido nucleico y metodo para incrementar el contenido de lisina treonina de las semillas de las plantas.

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Publication number
MX9301521A
MX9301521A MX9301521A MX9301521A MX9301521A MX 9301521 A MX9301521 A MX 9301521A MX 9301521 A MX9301521 A MX 9301521A MX 9301521 A MX9301521 A MX 9301521A MX 9301521 A MX9301521 A MX 9301521A
Authority
MX
Mexico
Prior art keywords
lysine
nucleic acid
increasing
content
treonin
Prior art date
Application number
MX9301521A
Other languages
English (en)
Inventor
Saverio Carl Falco
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of MX9301521A publication Critical patent/MX9301521A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se describe una subfragmento de ácido nucléico único que codifica para la aspartoquinasa insensible a la lisina (AK), que es capaz de provocar una producción mayor de la normal de treonina, cuando se liga a un subframento de ácido nucléico que codifica para la sintetasa del ácido dihidrodipicolíco, insensible a la lisina (DHDPS), mayor de la producción normal de lisina. Una modalidad adicional también incluye un subframento que codifica para la cetoglutarato-reductasa de la lisina, antisentido. Se proporciona las secuencias de ácido nucléico y los genesquiméricos, así como los métodos para incrementar el contenido de lisina o de semillas de plantas vegetales.
MX9301521A 1992-03-19 1993-03-18 Fragmentos de acido nucleico y metodo para incrementar el contenido de lisina treonina de las semillas de las plantas. MX9301521A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US85541492A 1992-03-19 1992-03-19

Publications (1)

Publication Number Publication Date
MX9301521A true MX9301521A (es) 1993-09-01

Family

ID=25321215

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9301521A MX9301521A (es) 1992-03-19 1993-03-18 Fragmentos de acido nucleico y metodo para incrementar el contenido de lisina treonina de las semillas de las plantas.

Country Status (9)

Country Link
EP (2) EP0640141B1 (es)
JP (1) JPH07504821A (es)
AU (1) AU675933B2 (es)
CA (1) CA2132414C (es)
DE (2) DE69334163T2 (es)
DK (2) DK1394257T3 (es)
MX (1) MX9301521A (es)
WO (1) WO1993019190A1 (es)
ZA (1) ZA931978B (es)

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US7705215B1 (en) * 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
EP0663921B1 (en) * 1992-10-05 2002-05-08 North Carolina State University Method for increasing expression and reducing expression variability of foreign genes in plant cells
US5661012A (en) * 1992-11-10 1997-08-26 Ajinomoto Co., Inc. Method for the production of L-threonine by fermentation, using mutated DNA encoding aspartokinase III
AU6162294A (en) * 1993-01-13 1994-08-15 Pioneer Hi-Bred International, Inc. High lysine derivatives of alpha-hordothionin
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
PL314696A1 (en) * 1993-11-30 1996-09-16 Du Pont Chiaeric genes and method of increasing lysine content in soya, maize and rape plant seeds
JPH07155184A (ja) * 1993-12-08 1995-06-20 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法
IL113685A0 (en) * 1994-05-13 1995-08-31 Du Pont Nucleic acid fragments chimeric genes and methods for increasing the methionine content of the seeds of plants
EP0769061A1 (en) * 1994-07-08 1997-04-23 E.I. Du Pont De Nemours And Company Chimeric genes and method for increasing the threonine content of the seeds of plants
JPH11506007A (ja) * 1995-05-31 1999-06-02 パイオニア ハイ−ブレッド インターナショナル，インコーポレイテッド 種子中の必須アミノ酸の蓄積を増加させる方法
CA2222600A1 (en) * 1995-06-02 1996-12-05 Pioneer Hi-Bred International, Inc. High methionine derivatives of .alpha.-hordothionin
EP0828835A1 (en) * 1995-06-02 1998-03-18 Pioneer Hi-Bred International, Inc. HIGH THREONINE DERIVATIVES OF $g(a)-HORDOTHIONIN
JP4004065B2 (ja) * 1995-06-13 2007-11-07 味の素株式会社 発酵法によるｌ−リジンの製造法
US6891084B1 (en) 1996-04-26 2005-05-10 Ajinomoto Co., Inc. DNA encoding raffinose synthase from soybean
US6166292A (en) * 1996-04-26 2000-12-26 Ajinomoto Co., Inc. Raffinose synthetase gene, method of producing raffinose and transgenic plant
JP4088982B2 (ja) * 1996-10-15 2008-05-21 味の素株式会社 発酵法によるｌ−アミノ酸の製造法
AU7509096A (en) * 1996-11-18 1998-06-10 Avebe Transgenic plant or plants with a naturally high water content overproducing at least two amino acids of the aspartate family
HUP0002305A3 (en) * 1997-03-27 2002-09-30 Du Pont Chimeric genes and methods for increasing the lysine content of the seeds of plants
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CN100529072C (zh) * 2001-09-05 2009-08-19 孟山都技术公司 用于植物中基因表达的种子特异性7Sα启动子
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US7157281B2 (en) * 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
CN1954076B (zh) 2004-04-06 2013-11-27 梅坦诺米克斯有限公司 产生精细化学品的方法
WO2006069610A2 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
EP2096177A3 (en) 2004-12-17 2010-01-13 Metanomics GmbH Process for the production of lutein
EP2194140A2 (en) 2005-03-02 2010-06-09 Metanomics GmbH Process for the production of fine chemicals
EP2573188A2 (en) 2005-03-02 2013-03-27 Metanomics GmbH Process for the production of fine chemicals
WO2007041419A1 (en) * 2005-10-03 2007-04-12 Monsanto Technology Llc Transgenic plant seed with increased lysine
EP2090662A3 (en) 2006-04-05 2012-10-31 Metanomics GmbH Process for the production of a fine chemical
BRPI0810083B8 (pt) 2007-03-28 2020-10-06 Monsanto Technology Llc métodos para estabelecer onde uma planta de soja ou semente de soja deve ser cultivada, de melhoramento da planta de soja, para selecionar uma semente de soja, de distribuição de uma planta de soja, para isolar sementes de soja de maturidade precoce indeterminada, e para determinar se uma planta de soja tem um grupo de maturidade
WO2009035852A2 (en) 2007-09-11 2009-03-19 Monsanto Technology Llc Increased alpha-prime beta-conglycinin soybeans
US9090882B2 (en) 2008-06-30 2015-07-28 Monsanto Technology Llc Compositions and methods for enhanced amino acid levels in plants conferred by lysine and/or threonine feedback insensitive aspartate kinase proteins
CN103773745B (zh) 2012-10-18 2018-03-23 中粮生物化学（安徽）股份有限公司 天冬氨酸激酶iii突变体及其宿主细胞和应用
CN106086011B (zh) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法
CN113201514B (zh) 2020-10-16 2022-09-06 中国科学院天津工业生物技术研究所 具有天冬氨酸激酶活性的多肽及其在生产氨基酸中的应用

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US5258300A (en) * 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
DE3943117A1 (de) * 1989-12-27 1991-07-04 Forschungszentrum Juelich Gmbh Verfahren zur fermentativen herstellung von aminosaeure, insbesondere l-lysin, dafuer geeignete mikroorganismen und rekombinante dna
EP0485970A3 (en) * 1990-11-13 1992-07-01 Yeda Research And Development Company Limited Transgenic plants overproducing threonine and lysine
US7746705B2 (en) * 2007-12-10 2010-06-29 Spansion Llc Selective application of word line bias to minimize fringe effects in electromagnetic fields during erase of nonvolatile memory

Also Published As

Publication number Publication date
DE69334163T2 (de) 2008-04-30
CA2132414A1 (en) 1993-09-30
DE69332974D1 (de) 2003-06-18
DE69332974T2 (de) 2004-05-19
AU3923393A (en) 1993-10-21
DK1394257T3 (da) 2007-12-10
JPH07504821A (ja) 1995-06-01
CA2132414C (en) 2009-01-27
EP1394257A1 (en) 2004-03-03
EP0640141B1 (en) 2003-05-14
WO1993019190A1 (en) 1993-09-30
AU675933B2 (en) 1997-02-27
ZA931978B (en) 1994-09-19
EP1394257B1 (en) 2007-08-08
DE69334163D1 (de) 2007-09-20
EP0640141A1 (en) 1995-03-01
DK0640141T3 (da) 2003-07-21

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