MX369986B - Metodos para identificar plantas de frijol tolerantes a sequia. - Google Patents

Metodos para identificar plantas de frijol tolerantes a sequia.

Info

Publication number
MX369986B
MX369986B MX2012006394A MX2012006394A MX369986B MX 369986 B MX369986 B MX 369986B MX 2012006394 A MX2012006394 A MX 2012006394A MX 2012006394 A MX2012006394 A MX 2012006394A MX 369986 B MX369986 B MX 369986B
Authority
MX
Mexico
Prior art keywords
plants
drought
bean
tolerant
molecular
Prior art date
Application number
MX2012006394A
Other languages
English (en)
Other versions
MX2012006394A (es
Inventor
Ruiz Medrano Roberto
Xoconostle Cázares Beatriz
Gómez Silva Lidia
Georgina Vargas López Aline
Toscano Morales Roberto
Original Assignee
Centro De Investig Y De Estudios Avanzados Del I P N Star
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro De Investig Y De Estudios Avanzados Del I P N Star filed Critical Centro De Investig Y De Estudios Avanzados Del I P N Star
Priority to MX2012006394A priority Critical patent/MX369986B/es
Priority to PCT/IB2013/054609 priority patent/WO2013182999A1/en
Publication of MX2012006394A publication Critical patent/MX2012006394A/es
Publication of MX369986B publication Critical patent/MX369986B/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/545—Phaseolus, e.g. kidney beans, scarlet runners or spotted beans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se refiere a métodos para identificar plantas de frijol tolerantes a sequía. Dicho método está basado en la evaluación molecular y fisiológica de plantas que son expuestas a estrés hídrico. Para la evaluación molecular se identifican y cuantifican los mRNAs de genes que se acumulan bajo estrés hídrico y para la evaluación fisiológica se midió la fotosíntesis. Para desarrollar la presente invención, se tomó como modelo de estudio la variedad de frijol Pinto Villa del cual por hibridación sustractiva supresiva se identificaron diversas secuencias nucleotídicas diferentes de 44 genes que se sobreexpresan en las plantas de frijol cuando éstas son expuestas a estrés hídrico, por lo que representa la utilidad de emplear dichas secuencias como marcadores moleculares útiles para identificar plantas de frijol tolerantes a sequía. Esta invención permite asistir la selección de plantas tolerantes a sequía con marcadores moleculares y parámetros fisiológicos en plántulas de frijol, sin tener que esperar a que ocurra todo su ciclo de vida, reduciendo sensiblemente el tiempo de identificación de las plantas tolerantes a sequía.
MX2012006394A 2012-06-04 2012-06-04 Metodos para identificar plantas de frijol tolerantes a sequia. MX369986B (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MX2012006394A MX369986B (es) 2012-06-04 2012-06-04 Metodos para identificar plantas de frijol tolerantes a sequia.
PCT/IB2013/054609 WO2013182999A1 (en) 2012-06-04 2013-06-04 Methods to identify drought-tolerant bean plants

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2012006394A MX369986B (es) 2012-06-04 2012-06-04 Metodos para identificar plantas de frijol tolerantes a sequia.

Publications (2)

Publication Number Publication Date
MX2012006394A MX2012006394A (es) 2013-12-17
MX369986B true MX369986B (es) 2018-11-13

Family

ID=48877290

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012006394A MX369986B (es) 2012-06-04 2012-06-04 Metodos para identificar plantas de frijol tolerantes a sequia.

Country Status (2)

Country Link
MX (1) MX369986B (es)
WO (1) WO2013182999A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108323275A (zh) * 2018-04-13 2018-07-27 成都大学 苦荞种子萌发抗旱胁迫装置及其胁迫方法
CN110656125A (zh) * 2019-09-23 2020-01-07 四川育良生物科技有限公司 一种耐干旱玉米的遗传转化方法
CN111955187B (zh) * 2020-05-29 2021-11-16 中国计量大学 利用相互嫁接体定量分析植物气孔关闭与根部基因型关系的方法
CN112980853A (zh) * 2021-03-11 2021-06-18 广西壮族自治区蚕业技术推广站 提高桑树抵御干旱胁迫的nced基因及其构建和用途
CN113151236B (zh) * 2021-04-20 2022-06-28 华中农业大学 玉米抗旱基因ZmcPGM2及其应用
CN114235882B (zh) * 2021-12-16 2024-03-15 河南省农业科学院芝麻研究中心 一种芝麻种质资源耐热性评价方法
CN114480702B (zh) * 2022-01-06 2024-01-16 青海大学 干旱胁迫下沙棘不同组织的荧光定量pcr内参基因及其筛选方法和应用
CN114839207A (zh) * 2022-04-12 2022-08-02 中国农业科学院棉花研究所 一种辅助鉴定棉花抗旱性的方法
CN117660523B (zh) * 2024-02-02 2024-04-16 河南大学三亚研究院 GhTSD7基因在提高植物干旱胁迫耐受性中的应用
CN118222760B (zh) * 2024-05-21 2024-08-23 浙江大学海南研究院 一种检测芥菜耐旱性状的InDel分子标记、引物组合和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101289514B (zh) 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 一种培育耐逆植物的方法及其专用dna片段
CN101508726B (zh) 2009-04-02 2011-10-19 中国农业大学 植物耐旱性相关蛋白及其编码基因与应用
CN101508728B (zh) 2009-04-02 2011-10-19 中国农业大学 一种植物耐旱性相关蛋白及其编码基因与应用

Also Published As

Publication number Publication date
MX2012006394A (es) 2013-12-17
WO2013182999A1 (en) 2013-12-12

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