MX369016B - Método de extracción de ácido desoxirribonucleico/ácido ribonucleico usando compuestos de amonio cuaternario sililados. - Google Patents

Método de extracción de ácido desoxirribonucleico/ácido ribonucleico usando compuestos de amonio cuaternario sililados.

Info

Publication number
MX369016B
MX369016B MX2015013638A MX2015013638A MX369016B MX 369016 B MX369016 B MX 369016B MX 2015013638 A MX2015013638 A MX 2015013638A MX 2015013638 A MX2015013638 A MX 2015013638A MX 369016 B MX369016 B MX 369016B
Authority
MX
Mexico
Prior art keywords
quaternary ammonium
dna
siqac
ammonium compounds
rna extraction
Prior art date
Application number
MX2015013638A
Other languages
English (en)
Other versions
MX2015013638A (es
Inventor
Benjamin David Cobb
Rogers Jan
Original Assignee
Arcis Biotechnology Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arcis Biotechnology Holdings Ltd filed Critical Arcis Biotechnology Holdings Ltd
Publication of MX2015013638A publication Critical patent/MX2015013638A/es
Publication of MX369016B publication Critical patent/MX369016B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente invención se refiere a un método para extraer ADN y/o ARN de una célula o cápside, el método Caracterizado porque comprende poner en contacto la célula o cápside con una composición acuosa que comprende un compuesto de amonio cuaternario que incluye un grupo funcional que contiene silicio; en donde la composición puesta en contacto con la célula o cápside es acuosa o secada por congelamiento; en donde el compuesto de amonio cuaternario que incluye un grupo funcional que contiene silicio es un compuesto de fórmula general (I) o una sal derivada del mismo en donde L es un grupo enlazante; cada uno de R1, R2, R3, R4, R5 y R6 se selecciona independientemente de H o un grupo alquilo, alquenilo, arilo o alcoxi opcionalmente sustituido; y n es 0 ó 1.
MX2015013638A 2013-03-25 2014-03-24 Método de extracción de ácido desoxirribonucleico/ácido ribonucleico usando compuestos de amonio cuaternario sililados. MX369016B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1305414.3A GB201305414D0 (en) 2013-03-25 2013-03-25 Method and composition
PCT/GB2014/050923 WO2014155078A1 (en) 2013-03-25 2014-03-24 Method of dna/rna extraction using silylated quaternary ammonium compounds (siqac)

Publications (2)

Publication Number Publication Date
MX2015013638A MX2015013638A (es) 2016-06-28
MX369016B true MX369016B (es) 2019-10-24

Family

ID=48326606

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015013638A MX369016B (es) 2013-03-25 2014-03-24 Método de extracción de ácido desoxirribonucleico/ácido ribonucleico usando compuestos de amonio cuaternario sililados.

Country Status (7)

Country Link
US (1) US10364427B2 (es)
EP (1) EP2978843B1 (es)
CN (1) CN105339496B (es)
BR (1) BR112015024465A2 (es)
GB (1) GB201305414D0 (es)
MX (1) MX369016B (es)
WO (1) WO2014155078A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201417372D0 (en) 2014-10-01 2014-11-12 Arcis Biotechnology Holdings Ltd Methods and kits
US10545152B2 (en) * 2015-07-30 2020-01-28 Arcis Biotechnology Holdings Limited Method and composition using a quaternary ammonium compound
GB201513492D0 (en) * 2015-07-30 2015-09-16 Arcis Biotechnology Holdings Ltd Method and composition
US20180355345A1 (en) * 2015-07-30 2018-12-13 Arcis Biotechnology Holdings Limited Method and composition
CN106148567B (zh) * 2016-07-08 2019-07-02 厦门出入境检验检疫局检验检疫技术中心 一种豇豆花叶病毒亚科病毒广谱检测试剂盒及其方法
CN106978484A (zh) * 2017-03-16 2017-07-25 北京博奥晶典生物技术有限公司 通过pcr添加剂解决等位基因扩增不平衡的方法
WO2018217852A1 (en) * 2017-05-23 2018-11-29 Gettysburg College Crispr based tool for characterizing bacterial serovar diversity
CN108559721A (zh) * 2018-05-15 2018-09-21 北京师范大学 一种净化空气的复合微生物菌剂及其应用
CN108410951B (zh) * 2018-05-24 2022-03-15 苏州先达基因科技有限公司 一种新的核酸提取试剂及其应用
GB202010371D0 (en) * 2020-07-06 2020-08-19 Agile Life Sciences Ltd Methods and uses relating to proteins
GB2596815A (en) * 2020-07-06 2022-01-12 Agile Life Sciences Ltd Kit and method

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
AU6230594A (en) * 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
DE10031236A1 (de) 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
US6852495B2 (en) * 2002-06-06 2005-02-08 Shimadzu Corporation Process of extracting nucleic acid and process of simultaneously carrying out extraction and purification of nucleic acid
EP1887863A2 (en) 2005-02-07 2008-02-20 SiShield Technologies Methods and compositions for biocidal treatments
US8501641B2 (en) 2007-10-01 2013-08-06 3M Innovative Properties Company Compositions comprising cationic fluorinated ether silanes, and related methods
JP4611446B1 (ja) 2010-01-19 2011-01-12 株式会社テラモト 表面多機能処理剤組成物
GB201003531D0 (en) * 2010-03-03 2010-04-21 Arcis Biotechnology Ltd Composition and method
ES2586037T3 (es) 2010-04-21 2016-10-11 Sabanci Universitesi Preparación de una composición de organosilano de amonio sustancialmente cuaternizado y solución acuosa autoestabilizante de la misma
GB201209229D0 (en) * 2012-05-25 2012-07-04 Epistem Ltd Nucleic acid extraction

Also Published As

Publication number Publication date
CN105339496A (zh) 2016-02-17
CN105339496B (zh) 2018-11-30
US10364427B2 (en) 2019-07-30
US20160040156A1 (en) 2016-02-11
EP2978843A1 (en) 2016-02-03
GB201305414D0 (en) 2013-05-08
BR112015024465A2 (pt) 2017-07-18
EP2978843B1 (en) 2018-10-31
WO2014155078A1 (en) 2014-10-02
MX2015013638A (es) 2016-06-28

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