MX365437B - Metodo para seleccionar una linea celular permisiva para replicar virus aviares. - Google Patents

Metodo para seleccionar una linea celular permisiva para replicar virus aviares.

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Publication number
MX365437B
MX365437B MX2016001041A MX2016001041A MX365437B MX 365437 B MX365437 B MX 365437B MX 2016001041 A MX2016001041 A MX 2016001041A MX 2016001041 A MX2016001041 A MX 2016001041A MX 365437 B MX365437 B MX 365437B
Authority
MX
Mexico
Prior art keywords
cell line
culturing
days
avian
selecting
Prior art date
Application number
MX2016001041A
Other languages
English (en)
Other versions
MX2016001041A (es
Inventor
François VAUTHEROT Jean-
Pain Bertrand
DENESVRE Caroline
Fragnet - Trapp Laetitia
Original Assignee
Agronomique Inst Nat Rech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech filed Critical Agronomique Inst Nat Rech
Publication of MX2016001041A publication Critical patent/MX2016001041A/es
Publication of MX365437B publication Critical patent/MX365437B/es

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/245—Herpetoviridae, e.g. herpes simplex virus
    • A61K39/255—Marek's disease virus
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5156—Animal cells expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5254—Virus avirulent or attenuated
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16311—Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
    • C12N2710/16334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16311—Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
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    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16311—Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
    • C12N2710/16351—Methods of production or purification of viral material
    • C12N2710/16352—Methods of production or purification of viral material relating to complementin g cells and packaging systems for producing virus or viral particles
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    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/10011—Birnaviridae
    • C12N2720/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/10011—Birnaviridae
    • C12N2720/10051—Methods of production or purification of viral material
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    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/10011—Birnaviridae
    • C12N2720/10051—Methods of production or purification of viral material
    • C12N2720/10052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Rheumatology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a un método para obtener una línea de células aviares no transformadas que permite la replicación de virus aviares in vitro, que comprende por lo menos los siguientes pasos: a) cultivar células madre embrionarias aviares en presencia de un estroma durante por lo menos 3 días; b) cultivar durante por lo menos 2 días en un medio con una concentración baja de suero; c) cultivar durante por lo menos 2 días en un medio de concentración baja de suero que comprende entre 1 y 10 mM de hexametilen bisacetamida (HMBA); d) cultivar durante por lo menos 10 días en un medio de concentración baja de suero; y e) cultivar o congelar una línea celular aviar permitiendo la replicación de virus aviares; la invención también se refiere a la línea celular resultante y al uso de la misma en preparaciones vacunales.
MX2016001041A 2013-07-25 2014-07-24 Metodo para seleccionar una linea celular permisiva para replicar virus aviares. MX365437B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1357346A FR3008992A1 (fr) 2013-07-25 2013-07-25 Procede de selection d'une lignee cellulaire permissive pour la replication de virus aviaires
PCT/EP2014/065946 WO2015011238A1 (fr) 2013-07-25 2014-07-24 Procede de sélection d'une lignee cellulaire permissive pour la replication de virus aviaires

Publications (2)

Publication Number Publication Date
MX2016001041A MX2016001041A (es) 2016-08-12
MX365437B true MX365437B (es) 2019-06-03

Family

ID=49876756

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016001041A MX365437B (es) 2013-07-25 2014-07-24 Metodo para seleccionar una linea celular permisiva para replicar virus aviares.

Country Status (11)

Country Link
US (1) US10053672B2 (es)
EP (1) EP3024927B1 (es)
CN (1) CN105408471B (es)
AU (1) AU2014295022B2 (es)
BR (1) BR112016001462A2 (es)
CA (1) CA2918845C (es)
ES (1) ES2662344T3 (es)
FR (1) FR3008992A1 (es)
MX (1) MX365437B (es)
NZ (1) NZ716434A (es)
WO (1) WO2015011238A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118956738B (zh) * 2024-09-12 2025-06-10 广州百赛生物医药科技有限公司 一种麻鸭胚胎成纤维细胞系及其构建方法与应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2253746T3 (es) 1995-07-03 2006-06-01 Akzo Nobel N.V. Vacuna contra la coccidiosis aviar.
US5827738A (en) * 1995-10-27 1998-10-27 Board Of Trustees Operating Michigan State University Sustainable chick cell line infected with Marek's disease virus
US5672485A (en) * 1996-08-13 1997-09-30 Regents Of The University Of Minnesota Immortalized cell lines for virus growth
FR2836924B1 (fr) * 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
FR2898908A1 (fr) * 2006-03-24 2007-09-28 Agronomique Inst Nat Rech Procede de preparation de cellules aviaires differenciees et genes impliques dans le maintien de la pluripotence
FR2898909A1 (fr) 2006-03-24 2007-09-28 Agronomique Inst Nat Rech Combinaison de marqueurs de cellules aviaires
EP1985305A1 (en) * 2007-04-24 2008-10-29 Vivalis Duck embryonic derived stem cell lines for the production of viral vaccines
CN103157107A (zh) * 2011-12-14 2013-06-19 普莱柯生物工程股份有限公司 用连续传代细胞系生产鸡马立克氏病疫苗及方法

Also Published As

Publication number Publication date
EP3024927A1 (fr) 2016-06-01
AU2014295022A1 (en) 2016-02-18
MX2016001041A (es) 2016-08-12
NZ716434A (en) 2022-01-28
CA2918845C (fr) 2021-11-02
US10053672B2 (en) 2018-08-21
EP3024927B1 (fr) 2017-12-27
US20160152956A1 (en) 2016-06-02
CA2918845A1 (fr) 2015-01-29
ES2662344T3 (es) 2018-04-06
FR3008992A1 (fr) 2015-01-30
CN105408471B (zh) 2019-01-18
AU2014295022B2 (en) 2020-05-28
WO2015011238A1 (fr) 2015-01-29
CN105408471A (zh) 2016-03-16
BR112016001462A2 (pt) 2017-08-29

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