MX354260B - Una tira de almohadilla individual para un ensayo de flujo lateral mejorado y un dispositivo de análisis que usa la misma. - Google Patents

Una tira de almohadilla individual para un ensayo de flujo lateral mejorado y un dispositivo de análisis que usa la misma.

Info

Publication number
MX354260B
MX354260B MX2014000830A MX2014000830A MX354260B MX 354260 B MX354260 B MX 354260B MX 2014000830 A MX2014000830 A MX 2014000830A MX 2014000830 A MX2014000830 A MX 2014000830A MX 354260 B MX354260 B MX 354260B
Authority
MX
Mexico
Prior art keywords
present
lateral flow
strip
same
flow assay
Prior art date
Application number
MX2014000830A
Other languages
English (en)
Other versions
MX2014000830A (es
Inventor
Hyeon Suk Kim
Tae-Hee Koo
Young Ho Choi
Original Assignee
Access Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Access Bio Inc filed Critical Access Bio Inc
Publication of MX2014000830A publication Critical patent/MX2014000830A/es
Publication of MX354260B publication Critical patent/MX354260B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/525—Multi-layer analytical elements
    • G01N33/526—Multi-layer analytical elements the element being adapted for a specific analyte
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/98—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving alcohol, e.g. ethanol in breath
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90209—Oxidoreductases (1.) acting on NADH or NADPH (1.6), e.g. those with a heme protein as acceptor (1.6.2) (general), Cytochrome-b5 reductase (1.6.2.2) or NADPH-cytochrome P450 reductase (1.6.2.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La presente invención se refiere a una tira para un ensayo de flujo lateral mejorado de una muestra biológica en un ensayo de cromatografía de flujo lateral y en un solo plano usando un dispositivo de análisis que contiene la misma. La tira de la presente invención consiste en una almohadilla individual, que puede mejorar el ensayo de flujo lateral proporcionando un procedimiento fácil y sencillo y una lectura visual clara. La tira de la presente invención consiste en una zona de aplicación de la muestra (muestra) y una zona de reactivo-resultante donde se deposita la mezcla de reacción (reactivo) que están en el mismo plano. Además, la presente invención proporciona un procedimiento cromatográfico en el que se separa la hemoglobina del analito por una cromatografía diferencial sobre la fase sólida. Cualquier interferencia de detección del resultado por la hemoglobina queda eliminada por la presente invención. La presente invención proporciona ventajas que incluyen un procedimiento fácil y sencillo con una respuesta rápida y clara. Una tira par un ensayo de enzima de flujo cromatográfico lateral para un analito preseleccionado en una muestra de sangre, caracterizada porque consiste en: una almohadilla única; y un sustrato depositado en forma seca, donde el analito comprende la enzima, donde la almohadilla única está pretratada con un tampón de lisis que comprende un detergente iónico o no-iónico, y donde la hemoglobina d ela muestra de sangre migra en la tira más rápido que el analito.
MX2014000830A 2011-07-22 2012-07-20 Una tira de almohadilla individual para un ensayo de flujo lateral mejorado y un dispositivo de análisis que usa la misma. MX354260B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161510762P 2011-07-22 2011-07-22
PCT/US2012/047625 WO2013016200A1 (en) 2011-07-22 2012-07-20 A single-pad strip for an improved lateral flow assay and a test device using the same

Publications (2)

Publication Number Publication Date
MX2014000830A MX2014000830A (es) 2014-07-09
MX354260B true MX354260B (es) 2018-02-21

Family

ID=47556024

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014000830A MX354260B (es) 2011-07-22 2012-07-20 Una tira de almohadilla individual para un ensayo de flujo lateral mejorado y un dispositivo de análisis que usa la misma.

Country Status (11)

Country Link
US (2) US9575056B2 (es)
EP (1) EP2734647B1 (es)
JP (1) JP6067701B2 (es)
KR (1) KR101751205B1 (es)
CN (2) CN108107211B (es)
BR (1) BR112014001544B1 (es)
CA (1) CA2841976C (es)
MX (1) MX354260B (es)
PH (1) PH12018500220A1 (es)
WO (1) WO2013016200A1 (es)
ZA (1) ZA201400481B (es)

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Also Published As

Publication number Publication date
JP2014525744A (ja) 2014-10-02
WO2013016200A1 (en) 2013-01-31
BR112014001544B1 (pt) 2020-10-06
EP2734647A4 (en) 2015-06-03
US9575056B2 (en) 2017-02-21
CN108107211B (zh) 2020-06-09
EP2734647B1 (en) 2018-10-17
PH12018500220B1 (en) 2018-10-15
MX2014000830A (es) 2014-07-09
PH12018500220A1 (en) 2018-10-15
CA2841976A1 (en) 2013-01-31
KR20140072023A (ko) 2014-06-12
EP2734647A1 (en) 2014-05-28
ZA201400481B (en) 2014-11-26
KR101751205B1 (ko) 2017-06-27
BR112014001544A2 (pt) 2017-06-13
CN103842523A (zh) 2014-06-04
US20130022969A1 (en) 2013-01-24
CA2841976C (en) 2021-05-11
US10048251B2 (en) 2018-08-14
US20170199177A1 (en) 2017-07-13
CN108107211A (zh) 2018-06-01
JP6067701B2 (ja) 2017-01-25

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