MX353959B - Metodo de floculacion. - Google Patents
Metodo de floculacion.Info
- Publication number
- MX353959B MX353959B MX2014007128A MX2014007128A MX353959B MX 353959 B MX353959 B MX 353959B MX 2014007128 A MX2014007128 A MX 2014007128A MX 2014007128 A MX2014007128 A MX 2014007128A MX 353959 B MX353959 B MX 353959B
- Authority
- MX
- Mexico
- Prior art keywords
- flocculation method
- polymers
- ionic
- cell culture
- mammalian cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000016615 flocculation Effects 0.000 title 1
- 238000005189 flocculation Methods 0.000 title 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 229920006317 cationic polymer Polymers 0.000 abstract 1
- 239000012930 cell culture fluid Substances 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 229920000831 ionic polymer Polymers 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 239000002736 nonionic surfactant Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
La presente invención se refiere a un método de cosecha de cultivo celular de mamífero, caracterizado porque comprende cultivar células de mamífero que expresan una proteína recombinante en un medio de cultivo celular por un tiempo predeterminado o hasta que se alcanza una densidad celular deseada y/o volumen celular empacado, agregar un polímero catiónico seleccionado de un polímero de cloruro de dialildimetilamonio o cloruro de polidalildimetilamonio y un polímero no iónico seleccionado de polietilenglicol y dextrano al medio de cultivo celular iniciando la floculación, mezclar el medio de cultivo celular durante la floculación, permitir que el floculante se sedimente par aun sedimento primario, y recuperar el sobrenadante primario clarificado.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161576303P | 2011-12-15 | 2011-12-15 | |
| PCT/US2012/069916 WO2013090820A1 (en) | 2011-12-15 | 2012-12-14 | Flocculation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014007128A MX2014007128A (es) | 2015-03-23 |
| MX353959B true MX353959B (es) | 2018-02-07 |
Family
ID=47559662
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014007128A MX353959B (es) | 2011-12-15 | 2012-12-14 | Metodo de floculacion. |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US9371554B2 (es) |
| EP (1) | EP2791319B1 (es) |
| JP (4) | JP2015504659A (es) |
| KR (5) | KR20220084192A (es) |
| CN (2) | CN104245922B (es) |
| AR (1) | AR089231A1 (es) |
| AU (4) | AU2012351926A1 (es) |
| BR (1) | BR112014014116A2 (es) |
| CA (1) | CA2859197C (es) |
| CL (1) | CL2014001564A1 (es) |
| EA (1) | EA201491144A1 (es) |
| HK (1) | HK1199057A1 (es) |
| IL (2) | IL233028B (es) |
| MX (1) | MX353959B (es) |
| SG (1) | SG11201403258VA (es) |
| TW (1) | TWI576144B (es) |
| WO (1) | WO2013090820A1 (es) |
| ZA (1) | ZA201404351B (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AR089231A1 (es) * | 2011-12-15 | 2014-08-06 | Amgen Inc | Metodo de floculacion |
| TW202246486A (zh) * | 2014-06-09 | 2022-12-01 | 美商健臻公司 | 種子罐培養法(seed train processes)及其用途 |
| FI20165282A (fi) | 2016-04-01 | 2017-10-02 | Kemira Oyj | Menetelmä ja järjestelmä koaguloinnin ja/tai flokkuloinnin optimoimiseksi veden käsittelyprosessissa |
| WO2017217930A1 (en) * | 2016-06-15 | 2017-12-21 | Agency For Science, Technology And Research | A method of enhancing the performance of chromatography methods for purification of proteins |
| WO2020044201A1 (en) * | 2018-08-30 | 2020-03-05 | Glaxosmithkline Intellectual Property Development Limited | Methods of detecting nucleic acid |
| HU231498B1 (hu) | 2019-04-04 | 2024-05-28 | Richter Gedeon Nyrt | Immunglobulinok affinitás kromatográfiájának fejlesztése kötést megelõzõ flokkulálás alkalmazásával |
| US11219843B2 (en) * | 2019-07-08 | 2022-01-11 | Nik Paya Karen Pharmed | Extraction of animal-derived pulmonary surfactants |
| CN111068367A (zh) * | 2019-12-17 | 2020-04-28 | 青岛宝和信生物科技有限公司 | 植物/中药提取液及水处理连续自动化絮凝及分离工艺 |
| GB2590721A (en) | 2019-12-31 | 2021-07-07 | Inst Jozef Stefan | Controlled aggregation of cells |
| EP4217366A1 (en) | 2020-09-22 | 2023-08-02 | Basf Se | Method for recovering a protein from a fermentation broth comprising a high degree of lysed cells |
| WO2024059235A2 (en) | 2022-09-16 | 2024-03-21 | Amgen Inc. | A method for harvesting products from perfusion cultures |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936694B1 (en) | 1982-05-06 | 2005-08-30 | Intermune, Inc. | Manufacture and expression of large structural genes |
| AU588819B2 (en) | 1984-10-29 | 1989-09-28 | Immunex Corporation | Cloning of human granulocyte-macrophage colony stimulating factor gene |
| US4968607A (en) | 1987-11-25 | 1990-11-06 | Immunex Corporation | Interleukin-1 receptors |
| US5075222A (en) | 1988-05-27 | 1991-12-24 | Synergen, Inc. | Interleukin-1 inhibitors |
| WO1990005183A1 (en) | 1988-10-31 | 1990-05-17 | Immunex Corporation | Interleukin-4 receptors |
| US5395760A (en) | 1989-09-05 | 1995-03-07 | Immunex Corporation | DNA encoding tumor necrosis factor-α and -β receptors |
| EP0939121B2 (de) | 1989-09-12 | 2007-12-26 | AHP Manufacturing B.V. | TFN-bindende Proteine |
| US6204363B1 (en) | 1989-10-16 | 2001-03-20 | Amgen Inc. | Stem cell factor |
| US5149792A (en) | 1989-12-19 | 1992-09-22 | Amgen Inc. | Platelet-derived growth factor B chain analogs |
| US5272064A (en) | 1989-12-19 | 1993-12-21 | Amgen Inc. | DNA molecules encoding platelet-derived growth factor B chain analogs and method for expression thereof |
| US5350683A (en) | 1990-06-05 | 1994-09-27 | Immunex Corporation | DNA encoding type II interleukin-1 receptors |
| AU651596B2 (en) | 1990-06-05 | 1994-07-28 | Immunex Corporation | Type II interleukin-1 receptors |
| EP0672141B1 (en) | 1992-10-23 | 2003-05-14 | Immunex Corporation | Methods of preparing soluble, oligomeric proteins |
| US5554512A (en) | 1993-05-24 | 1996-09-10 | Immunex Corporation | Ligands for flt3 receptors |
| US5552316A (en) * | 1993-06-04 | 1996-09-03 | Environmental Marketing Services, Ltd. | Clarifying E. coli fermentation broths with a combination of anionic and cationic flocculants |
| US5981713A (en) | 1994-10-13 | 1999-11-09 | Applied Research Systems Ars Holding N.V. | Antibodies to intereleukin-1 antagonists |
| ATE310813T1 (de) | 1995-06-29 | 2005-12-15 | Immunex Corp | Apoptosis induzierendes cytokin |
| US6613544B1 (en) | 1995-12-22 | 2003-09-02 | Amgen Inc. | Osteoprotegerin |
| US6096728A (en) | 1996-02-09 | 2000-08-01 | Amgen Inc. | Composition and method for treating inflammatory diseases |
| US6271349B1 (en) | 1996-12-23 | 2001-08-07 | Immunex Corporation | Receptor activator of NF-κB |
| US6235883B1 (en) | 1997-05-05 | 2001-05-22 | Abgenix, Inc. | Human monoclonal antibodies to epidermal growth factor receptor |
| US6337072B1 (en) | 1998-04-03 | 2002-01-08 | Hyseq, Inc. | Interleukin-1 receptor antagonist and recombinant production thereof |
| GB9919187D0 (en) | 1999-08-14 | 1999-10-20 | Ciba Spec Chem Water Treat Ltd | Flocculation of cell material |
| WO2001036637A1 (en) | 1999-11-17 | 2001-05-25 | Immunex Corporation | Receptor activator of nf-kappa b |
| EP1403274A1 (en) * | 2002-09-30 | 2004-03-31 | Meristem Therapeutics | Process for the purification of recombinant proteins from complex media and purified proteins obtained thereby |
| TW200801039A (en) * | 2005-09-15 | 2008-01-01 | Wyeth Corp | Separation methods |
| WO2008100578A2 (en) | 2007-02-14 | 2008-08-21 | Amgen Inc. | Method of isolating antibodies by precipitation |
| EP1985305A1 (en) * | 2007-04-24 | 2008-10-29 | Vivalis | Duck embryonic derived stem cell lines for the production of viral vaccines |
| CN101946003B (zh) * | 2008-02-14 | 2013-05-15 | 株式会社遗传科技 | 抗adam-15抗体及其应用 |
| CN102272146A (zh) * | 2009-01-13 | 2011-12-07 | 通用电气健康护理生物科学股份公司 | 用带负电的聚合物沉淀生物分子 |
| AR089231A1 (es) | 2011-12-15 | 2014-08-06 | Amgen Inc | Metodo de floculacion |
-
2012
- 2012-12-13 AR ARP120104705A patent/AR089231A1/es unknown
- 2012-12-14 EA EA201491144A patent/EA201491144A1/ru unknown
- 2012-12-14 CN CN201280069842.1A patent/CN104245922B/zh active Active
- 2012-12-14 KR KR1020227019427A patent/KR20220084192A/ko active Application Filing
- 2012-12-14 KR KR1020237042787A patent/KR20230172040A/ko active Application Filing
- 2012-12-14 BR BR112014014116A patent/BR112014014116A2/pt not_active Application Discontinuation
- 2012-12-14 EP EP12815893.8A patent/EP2791319B1/en active Active
- 2012-12-14 SG SG11201403258VA patent/SG11201403258VA/en unknown
- 2012-12-14 AU AU2012351926A patent/AU2012351926A1/en not_active Abandoned
- 2012-12-14 CN CN202110381379.5A patent/CN113150060A/zh active Pending
- 2012-12-14 US US14/365,496 patent/US9371554B2/en active Active
- 2012-12-14 WO PCT/US2012/069916 patent/WO2013090820A1/en active Application Filing
- 2012-12-14 TW TW101147676A patent/TWI576144B/zh active
- 2012-12-14 KR KR1020197035609A patent/KR20190137944A/ko not_active IP Right Cessation
- 2012-12-14 JP JP2014547521A patent/JP2015504659A/ja active Pending
- 2012-12-14 MX MX2014007128A patent/MX353959B/es active IP Right Grant
- 2012-12-14 CA CA2859197A patent/CA2859197C/en active Active
- 2012-12-14 KR KR1020217023322A patent/KR20210095723A/ko not_active Application Discontinuation
- 2012-12-14 KR KR1020147019193A patent/KR20140103320A/ko not_active Application Discontinuation
-
2014
- 2014-06-09 IL IL233028A patent/IL233028B/en active IP Right Grant
- 2014-06-12 ZA ZA2014/04351A patent/ZA201404351B/en unknown
- 2014-06-13 CL CL2014001564A patent/CL2014001564A1/es unknown
- 2014-12-09 HK HK14112366.5A patent/HK1199057A1/xx unknown
-
2016
- 2016-06-20 US US15/187,460 patent/US20160297850A1/en not_active Abandoned
-
2018
- 2018-06-22 AU AU2018204547A patent/AU2018204547B2/en active Active
- 2018-10-12 JP JP2018193329A patent/JP6998853B2/ja active Active
- 2018-12-12 IL IL263674A patent/IL263674A/en unknown
-
2020
- 2020-11-09 AU AU2020267147A patent/AU2020267147B2/en active Active
-
2021
- 2021-09-24 JP JP2021155652A patent/JP7274547B2/ja active Active
-
2023
- 2023-04-28 JP JP2023074144A patent/JP2023099117A/ja active Pending
- 2023-08-23 AU AU2023219874A patent/AU2023219874A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX353959B (es) | Metodo de floculacion. | |
| IL288509A (en) | Mammalian cell culture | |
| MX2021008143A (es) | Metodos para cosechar cultivos de celulas de mamifero. | |
| MX2019003427A (es) | Composiciones para cultivo celular y metodos para la produccion de polipeptidos. | |
| EA201171018A1 (ru) | Биореактор для культивирования клеток млекопитающих | |
| FR2974814B1 (fr) | Photobioreacteur en milieu ferme pour la culture de micro-organismes photosynthetiques | |
| MY186219A (en) | Production of proteins in glutamine-free cell culture media | |
| MX2013003681A (es) | Ácidos nucleicos manipulados y métodos de uso de los mismos. | |
| SG10201805291TA (en) | Production of heterodimeric proteins | |
| SG10201811616SA (en) | Cell culture media | |
| SG10201809452RA (en) | Cell culture compositions with antioxidants and methods for polypeptide production | |
| MX2015003700A (es) | Celda para electrolizar un liquido. | |
| EA201492185A1 (ru) | Способ получения рекомбинантной идуронат-2-сульфатазы | |
| IN2014CN02963A (es) | ||
| WO2011119986A3 (en) | Methods for culturing and analyzing cells | |
| IN2014DN09097A (es) | ||
| EP2820123A4 (en) | METHOD FOR CONTROLLING THE RECOVERY OF ENDOTHELIAL CELLS FROM HUMAN PLURIPOTENTIAL STEM CELLS WITH A TWO-DIMENSIONAL FEEDER-FREE DIFFERENTIATION | |
| EA201300884A8 (ru) | Способ получения нового антибактериального лекарственного средства на основе рекомбинантного полипептида | |
| EP2905342A4 (en) | METHOD FOR PREVENTING POLYPEPTIDE REDUCTION BY ADDITION OF AMINO ACID TO A LIQUID CULTURE MEDIUM | |
| EA201201620A1 (ru) | Способ получения рнк-вируса | |
| UA53819U (ru) | Технологический комплекс по производству молока | |
| UA64452U (ru) | Применение гена аполипопротеида Е (апоЕ) в качестве средства для профилактики кардиосклероза | |
| MX346721B (es) | Proceso mejorado para la obtención de galphiminas (a,b y e) con actividad ansiolitica a partir del cultivo in vitro de galphimia glauca. | |
| GB201105503D0 (en) | Microcarrier for the culture if stem cells, method of culturing stem cells and method of using said microcarrier |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |