MX2022015461A - Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos. - Google Patents

Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos.

Info

Publication number
MX2022015461A
MX2022015461A MX2022015461A MX2022015461A MX2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A
Authority
MX
Mexico
Prior art keywords
sample
antibodies
liquid chromatography
free
spectrometry
Prior art date
Application number
MX2022015461A
Other languages
English (en)
Inventor
Shunhai Wang
Yuetian Yan
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2022015461A publication Critical patent/MX2022015461A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se proporcionan métodos sin cromatografía líquida para cuantificar una proteína diana en una muestra. Una forma de realización proporciona un método sin cromatografía líquida para cuantificar anticuerpos diana en una muestra que incluye las etapas de agregar a la muestra un anticuerpo estándar interno etiquetado, digerir los anticuerpos en la muestra para producir péptidos, fraccionar los péptidos; y cuantificar los anticuerpos diana mediante el uso de un sistema MS2 de infusión directa que contiene una o más trampas de iones y dos o más filtros de masas cuadripolares y un ionizador de electropulverización, en donde el método no comprende cromatografía líquida.
MX2022015461A 2020-06-09 2021-06-07 Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos. MX2022015461A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063036679P 2020-06-09 2020-06-09
PCT/US2021/036170 WO2021252357A1 (en) 2020-06-09 2021-06-07 A high-throughput and mass-spectrometry-based method for quantitating antibodies

Publications (1)

Publication Number Publication Date
MX2022015461A true MX2022015461A (es) 2023-01-16

Family

ID=78817337

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022015461A MX2022015461A (es) 2020-06-09 2021-06-07 Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos.

Country Status (11)

Country Link
US (1) US20210382067A1 (es)
EP (1) EP4162250A1 (es)
JP (1) JP2023536681A (es)
KR (1) KR20230020996A (es)
CN (1) CN115769056A (es)
AU (1) AU2021286463A1 (es)
BR (1) BR112022024978A2 (es)
CA (1) CA3179460A1 (es)
IL (1) IL298781A (es)
MX (1) MX2022015461A (es)
WO (1) WO2021252357A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR127006A1 (es) * 2021-09-08 2023-12-06 Regeneron Pharma UN MÉTODO BASADO EN ESPECTROMETRÍA DE MASAS Y DE ALTO RENDIMIENTO PARA CUANTIFICAR ANTICUERPOS Y OTRAS PROTEÍNAS QUE CONTIENEN Fc

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2426499A1 (en) * 2010-09-03 2012-03-07 Bruker Daltonik GmbH Immunosuppressant monitoring by MALDI mass spectrometry
JP5987053B2 (ja) * 2011-05-12 2016-09-06 ジェネンテック， インコーポレイテッド フレームワークシグネチャーペプチドを用いて動物サンプルにおける治療抗体を検出するための多重反応モニタリングｌｃ−ｍｓ／ｍｓ法
HRPK20130568B3 (hr) * 2013-06-20 2016-01-29 Genos D.O.O. Postupak analize n-glikana vezanih na imunoglobulin g iz krvne plazme ljudi i njegova upotreba
WO2016018978A1 (en) * 2014-07-29 2016-02-04 Mayo Foundation For Medical Education And Research Quantifying monoclonal antibody therapeutics by lc-ms/ms
GB2567793B (en) * 2017-04-13 2023-03-22 Micromass Ltd A method of fragmenting and charge reducing biomolecules
JP2022517338A (ja) * 2019-01-16 2022-03-08 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 抗体の断片化を特定及び定量する方法及びシステム

Also Published As

Publication number Publication date
US20210382067A1 (en) 2021-12-09
AU2021286463A1 (en) 2022-12-22
IL298781A (en) 2023-02-01
KR20230020996A (ko) 2023-02-13
CA3179460A1 (en) 2021-12-16
WO2021252357A1 (en) 2021-12-16
JP2023536681A (ja) 2023-08-29
EP4162250A1 (en) 2023-04-12
CN115769056A (zh) 2023-03-07
BR112022024978A2 (pt) 2023-02-28

Similar Documents

Publication Publication Date Title
Wöhlbrand et al. Proteomic tools for environmental microbiology—a roadmap from sample preparation to protein identification and quantification
US20020182649A1 (en) Methods for protein identification, characterization and sequencing by tandem mass spectrometry
DE602005023278D1 (de) Elektronentransferdissoziation zur biopolymer-sequenzanalyse
CN108604525B (zh) 用于靶向ms方法中的自适应保留时间的前哨信号
US20160041184A1 (en) Identification and monitoring of monoclonal immunoglobulins by molecular mass
Sun et al. Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for quantitative parallel reaction monitoring of peptide abundance and single-shot proteomic analysis of a human cell line
Loo et al. Proteomics in molecular diagnosis: typing of amyloidosis
Zhu et al. Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE‐ESI‐MS/MS system
KR20210153102A (ko) 숙주세포 단백질의 확인
MX2022015461A (es) Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos.
US20200249241A1 (en) Native Microfluidic CE-MS Analysis of Antibody Charge Heterogeneity
Chiu et al. Desalting paper spray mass spectrometry (DPS-MS) for rapid detection of glycans and glycoconjugates
Kim et al. Mapping protein receptor–ligand interactions via in vivo chemical crosslinking, affinity purification, and differential mass spectrometry
CN110687297A (zh) 基于稳定同位素半胱氨酸代谢标记的棕榈酰化修饰蛋白质定量分析方法
Bensaddek et al. Quantitative proteomic analysis of the human nucleolus
Schönberger et al. Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers
Segura et al. Ultrafast analysis of peptides by laser diode thermal desorption–triple quadrupole mass spectrometry
JP2022517414A (ja) 無傷のタンパク質レベルでのＬＣ－ＭＳに基づくＨｂＡ１ｃ測定の自動化試料ワークフロー
Shimazaki et al. Combined method of immunoaffinity membrane within tubes and MALDI-TOF MS for capturing and analyzing amyloid beta
AR127006A1 (es) UN MÉTODO BASADO EN ESPECTROMETRÍA DE MASAS Y DE ALTO RENDIMIENTO PARA CUANTIFICAR ANTICUERPOS Y OTRAS PROTEÍNAS QUE CONTIENEN Fc
Schoenherr et al. A dataset describing a suite of novel antibody reagents for the RAS signaling network
Bergquist et al. Future aspects of psychoneuroimmunology–lymphocyte peptides reflecting psychiatric disorders studied by mass spectrometry
Vaezzadeh et al. Imaging mass spectrometry using peptide isoelectric focusing
Bennett DOSCATs: Double Standards in Quantitative Proteomics
Wu et al. Localizing Isomerized Residue Sites in Peptides with Tandem Mass Spectrometry