MX2022015461A - Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos. - Google Patents
Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos.Info
- Publication number
- MX2022015461A MX2022015461A MX2022015461A MX2022015461A MX2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A MX 2022015461 A MX2022015461 A MX 2022015461A
- Authority
- MX
- Mexico
- Prior art keywords
- sample
- antibodies
- liquid chromatography
- free
- spectrometry
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000004949 mass spectrometry Methods 0.000 title 1
- 239000007788 liquid Substances 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 238000001802 infusion Methods 0.000 abstract 1
- 238000005040 ion trap Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000012421 spiking Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Se proporcionan métodos sin cromatografía líquida para cuantificar una proteína diana en una muestra. Una forma de realización proporciona un método sin cromatografía líquida para cuantificar anticuerpos diana en una muestra que incluye las etapas de agregar a la muestra un anticuerpo estándar interno etiquetado, digerir los anticuerpos en la muestra para producir péptidos, fraccionar los péptidos; y cuantificar los anticuerpos diana mediante el uso de un sistema MS2 de infusión directa que contiene una o más trampas de iones y dos o más filtros de masas cuadripolares y un ionizador de electropulverización, en donde el método no comprende cromatografía líquida.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063036679P | 2020-06-09 | 2020-06-09 | |
PCT/US2021/036170 WO2021252357A1 (en) | 2020-06-09 | 2021-06-07 | A high-throughput and mass-spectrometry-based method for quantitating antibodies |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2022015461A true MX2022015461A (es) | 2023-01-16 |
Family
ID=78817337
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2022015461A MX2022015461A (es) | 2020-06-09 | 2021-06-07 | Metodo basado en espectrometria de masas y de alto rendimiento para cuantificar anticuerpos. |
Country Status (11)
Country | Link |
---|---|
US (1) | US20210382067A1 (es) |
EP (1) | EP4162250A1 (es) |
JP (1) | JP2023536681A (es) |
KR (1) | KR20230020996A (es) |
CN (1) | CN115769056A (es) |
AU (1) | AU2021286463A1 (es) |
BR (1) | BR112022024978A2 (es) |
CA (1) | CA3179460A1 (es) |
IL (1) | IL298781A (es) |
MX (1) | MX2022015461A (es) |
WO (1) | WO2021252357A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20230077710A1 (en) * | 2021-09-08 | 2023-03-16 | Regeneron Pharmaceuticals, Inc. | HIGH-THROUGHPUT AND MASS-SPECTROMETRY-BASED METHOD FOR QUANTITATING ANTIBODIES AND OTHER Fc-CONTAINING PROTEINS |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2426499A1 (en) * | 2010-09-03 | 2012-03-07 | Bruker Daltonik GmbH | Immunosuppressant monitoring by MALDI mass spectrometry |
CA2833212C (en) * | 2011-05-12 | 2020-06-09 | Genentech, Inc. | Multiple reaction monitoring lc-ms/ms method to detect therapeutic antibodies in animal samples using framework signature peptides |
HRPK20130568B3 (hr) * | 2013-06-20 | 2016-01-29 | Genos D.O.O. | Postupak analize n-glikana vezanih na imunoglobulin g iz krvne plazme ljudi i njegova upotreba |
US10690676B2 (en) * | 2014-07-29 | 2020-06-23 | Mayo Roundation for Medical Education and Research | Quantifying monoclonal antibody therapeutics by LC-MS/MS |
GB2567793B (en) * | 2017-04-13 | 2023-03-22 | Micromass Ltd | A method of fragmenting and charge reducing biomolecules |
MX2021008558A (es) * | 2019-01-16 | 2021-08-19 | Regeneron Pharma | Metodo y sistema para la identificacion y cuantificacion de la fragmentacion de anticuerpos. |
-
2021
- 2021-06-07 KR KR1020227043147A patent/KR20230020996A/ko unknown
- 2021-06-07 JP JP2022575348A patent/JP2023536681A/ja active Pending
- 2021-06-07 IL IL298781A patent/IL298781A/en unknown
- 2021-06-07 MX MX2022015461A patent/MX2022015461A/es unknown
- 2021-06-07 AU AU2021286463A patent/AU2021286463A1/en active Pending
- 2021-06-07 BR BR112022024978A patent/BR112022024978A2/pt unknown
- 2021-06-07 CA CA3179460A patent/CA3179460A1/en active Pending
- 2021-06-07 WO PCT/US2021/036170 patent/WO2021252357A1/en unknown
- 2021-06-07 EP EP21821732.1A patent/EP4162250A1/en active Pending
- 2021-06-07 CN CN202180040944.XA patent/CN115769056A/zh active Pending
- 2021-06-08 US US17/341,746 patent/US20210382067A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
KR20230020996A (ko) | 2023-02-13 |
US20210382067A1 (en) | 2021-12-09 |
EP4162250A1 (en) | 2023-04-12 |
CA3179460A1 (en) | 2021-12-16 |
BR112022024978A2 (pt) | 2023-02-28 |
IL298781A (en) | 2023-02-01 |
JP2023536681A (ja) | 2023-08-29 |
WO2021252357A1 (en) | 2021-12-16 |
AU2021286463A1 (en) | 2022-12-22 |
CN115769056A (zh) | 2023-03-07 |
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