BR112022024978A2 - Métodos livre de cromatografia líquida para quantificar anticorpos alvo em uma amostra e para quantificar um produto de droga de proteína em uma amostra biológica - Google Patents

Métodos livre de cromatografia líquida para quantificar anticorpos alvo em uma amostra e para quantificar um produto de droga de proteína em uma amostra biológica

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Publication number
BR112022024978A2
BR112022024978A2 BR112022024978A BR112022024978A BR112022024978A2 BR 112022024978 A2 BR112022024978 A2 BR 112022024978A2 BR 112022024978 A BR112022024978 A BR 112022024978A BR 112022024978 A BR112022024978 A BR 112022024978A BR 112022024978 A2 BR112022024978 A2 BR 112022024978A2
Authority
BR
Brazil
Prior art keywords
sample
quantify
liquid chromatography
target antibodies
chromatography methods
Prior art date
Application number
BR112022024978A
Other languages
English (en)
Inventor
Yan Yuetian
Wang Shunhai
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of BR112022024978A2 publication Critical patent/BR112022024978A2/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

MÉTODOS LIVRE DE CROMATOGRAFIA LÍQUIDA PARA QUANTIFICAR ANTICORPOS ALVO EM UMA AMOSTRA E PARA QUANTIFICAR UM PRODUTO DE DROGA DE PROTEÍNA EM UMA AMOSTRA BIOLÓGICA. Métodos livres de cromatografia líquida para quantificar uma proteína alvo em uma amostra são fornecidos. Uma modalidade fornece um método livre de cromatografia líquida para quantificar anticorpos alvo em uma amostra, incluindo as etapas de aumentar a amostra com um anticorpo padrão interno marcado, digerir os anticorpos na amostra para produzir peptídeos, fracionar os peptídeos; e quantificar os anticorpos alvo usando um sistema MS2 de infusão direta contendo uma ou mais armadilhas de íons e dois ou mais filtros de massa quadripolo e um ionizador de eletropulverização, em que o método é livre de cromatografia líquida.
BR112022024978A 2020-06-09 2021-06-07 Métodos livre de cromatografia líquida para quantificar anticorpos alvo em uma amostra e para quantificar um produto de droga de proteína em uma amostra biológica BR112022024978A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063036679P 2020-06-09 2020-06-09
PCT/US2021/036170 WO2021252357A1 (en) 2020-06-09 2021-06-07 A high-throughput and mass-spectrometry-based method for quantitating antibodies

Publications (1)

Publication Number Publication Date
BR112022024978A2 true BR112022024978A2 (pt) 2023-02-28

Family

ID=78817337

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022024978A BR112022024978A2 (pt) 2020-06-09 2021-06-07 Métodos livre de cromatografia líquida para quantificar anticorpos alvo em uma amostra e para quantificar um produto de droga de proteína em uma amostra biológica

Country Status (11)

Country Link
US (1) US20210382067A1 (pt)
EP (1) EP4162250A1 (pt)
JP (1) JP2023536681A (pt)
KR (1) KR20230020996A (pt)
CN (1) CN115769056A (pt)
AU (1) AU2021286463A1 (pt)
BR (1) BR112022024978A2 (pt)
CA (1) CA3179460A1 (pt)
IL (1) IL298781A (pt)
MX (1) MX2022015461A (pt)
WO (1) WO2021252357A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023039457A1 (en) * 2021-09-08 2023-03-16 Regeneron Pharmaceuticals, Inc. A high-throughput and mass-spectrometry-based method for quantitating antibodies and other fc-containing proteins

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2426499A1 (en) * 2010-09-03 2012-03-07 Bruker Daltonik GmbH Immunosuppressant monitoring by MALDI mass spectrometry
CA2833212C (en) * 2011-05-12 2020-06-09 Genentech, Inc. Multiple reaction monitoring lc-ms/ms method to detect therapeutic antibodies in animal samples using framework signature peptides
HRPK20130568B3 (hr) * 2013-06-20 2016-01-29 Genos D.O.O. Postupak analize n-glikana vezanih na imunoglobulin g iz krvne plazme ljudi i njegova upotreba
WO2016018978A1 (en) * 2014-07-29 2016-02-04 Mayo Foundation For Medical Education And Research Quantifying monoclonal antibody therapeutics by lc-ms/ms
GB2567793B (en) * 2017-04-13 2023-03-22 Micromass Ltd A method of fragmenting and charge reducing biomolecules
EA202191961A1 (ru) * 2019-01-16 2021-12-13 Ридженерон Фармасьютикалз, Инк. Способ и система идентификации и количественного определения фрагментации антител

Also Published As

Publication number Publication date
MX2022015461A (es) 2023-01-16
CN115769056A (zh) 2023-03-07
WO2021252357A1 (en) 2021-12-16
AU2021286463A1 (en) 2022-12-22
CA3179460A1 (en) 2021-12-16
IL298781A (en) 2023-02-01
KR20230020996A (ko) 2023-02-13
US20210382067A1 (en) 2021-12-09
EP4162250A1 (en) 2023-04-12
JP2023536681A (ja) 2023-08-29

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