MX2021008558A - Metodo y sistema para la identificacion y cuantificacion de la fragmentacion de anticuerpos. - Google Patents
Metodo y sistema para la identificacion y cuantificacion de la fragmentacion de anticuerpos.Info
- Publication number
- MX2021008558A MX2021008558A MX2021008558A MX2021008558A MX2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A MX 2021008558 A MX2021008558 A MX 2021008558A
- Authority
- MX
- Mexico
- Prior art keywords
- identifying
- fragments
- antibody
- mixed
- molecular weight
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
- G01N30/724—Nebulising, aerosol formation or ionisation
- G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Métodos para identificar y cuantificar fragmentos de anticuerpo e identificar el sitio de fragmentación de un anticuerpo en una muestra que comprende las etapas de cargar una muestra que incluye fragmentos del anticuerpo en un sistema cromatográfico que tiene una fase estacionaria (110) de exclusión por tamaño de modo mixto, lavar dicha fase estacionaria de exclusión por tamaño de modo mixto mediante el uso de una fase móvil para proporcionar un eluyente que comprende dichos fragmentos, determinar el peso molecular de dichos fragmentos mediante el uso de un espectrómetro de masas (120), y correlacionar dicho peso molecular con estándares proteicos conocidos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962793004P | 2019-01-16 | 2019-01-16 | |
PCT/US2020/013648 WO2020150328A1 (en) | 2019-01-16 | 2020-01-15 | Method and system of identifying and quantifying antibody fragmentation |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2021008558A true MX2021008558A (es) | 2021-08-19 |
Family
ID=69529055
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2021008558A MX2021008558A (es) | 2019-01-16 | 2020-01-15 | Metodo y sistema para la identificacion y cuantificacion de la fragmentacion de anticuerpos. |
Country Status (13)
Country | Link |
---|---|
US (2) | US11486864B2 (es) |
EP (1) | EP3911947A1 (es) |
JP (1) | JP2022517338A (es) |
KR (1) | KR20210116502A (es) |
CN (1) | CN113227785A (es) |
AU (1) | AU2020208360A1 (es) |
BR (1) | BR112021011194A2 (es) |
CA (1) | CA3123052A1 (es) |
EA (1) | EA202191961A1 (es) |
IL (1) | IL284637A (es) |
MX (1) | MX2021008558A (es) |
SG (1) | SG11202106370WA (es) |
WO (1) | WO2020150328A1 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11385239B2 (en) * | 2018-10-31 | 2022-07-12 | Regeneran Pharmaceuticals, Inc. | Method and system of identifying and quantifying a protein |
AU2021286463A1 (en) * | 2020-06-09 | 2022-12-22 | Regeneron Pharmaceuticals, Inc. | A high-throughput and mass-spectrometry-based method for quantitating antibodies |
US20230018713A1 (en) * | 2021-07-13 | 2023-01-19 | Regeneron Pharmaceuticals, Inc. | Characterization of proteins by anion-exchange chromatography mass spectrometry (aex-ms) |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2493817A1 (en) * | 2002-08-06 | 2004-02-12 | The John Hopkins University | Use of biomarkers for detecting ovarian cancer |
WO2007038523A2 (en) * | 2005-09-27 | 2007-04-05 | Center For Applied Proteomics And Molecular Medicine | Method of isolating analytes from a sample |
MX2015006868A (es) * | 2012-11-30 | 2015-10-05 | Novartis Ag | Metodos para la elaboracion de conjugados a partir de proteinas que contienen disulfuro. |
US11092574B2 (en) | 2013-12-24 | 2021-08-17 | Waters Technologies Corporation | Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides |
WO2016018978A1 (en) | 2014-07-29 | 2016-02-04 | Mayo Foundation For Medical Education And Research | Quantifying monoclonal antibody therapeutics by lc-ms/ms |
WO2016149088A1 (en) * | 2015-03-13 | 2016-09-22 | Bristol-Myers Squibb Company | Use of alkaline washes during chromatography to remove impurities |
CA3013340A1 (en) * | 2016-02-04 | 2017-08-10 | Oncobiologics, Inc. | Methods for identifying and analyzing amino acid sequences of proteins |
MX2019000935A (es) * | 2016-08-16 | 2019-07-04 | Regeneron Pharma | Metodos para cuantificar anticuerpos individuales de una mezcla. |
US11385239B2 (en) * | 2018-10-31 | 2022-07-12 | Regeneran Pharmaceuticals, Inc. | Method and system of identifying and quantifying a protein |
-
2020
- 2020-01-15 EA EA202191961A patent/EA202191961A1/ru unknown
- 2020-01-15 CN CN202080007050.6A patent/CN113227785A/zh active Pending
- 2020-01-15 WO PCT/US2020/013648 patent/WO2020150328A1/en unknown
- 2020-01-15 KR KR1020217024445A patent/KR20210116502A/ko unknown
- 2020-01-15 AU AU2020208360A patent/AU2020208360A1/en active Pending
- 2020-01-15 US US16/743,296 patent/US11486864B2/en active Active
- 2020-01-15 EP EP20704735.8A patent/EP3911947A1/en active Pending
- 2020-01-15 BR BR112021011194-8A patent/BR112021011194A2/pt not_active Application Discontinuation
- 2020-01-15 SG SG11202106370WA patent/SG11202106370WA/en unknown
- 2020-01-15 JP JP2021539900A patent/JP2022517338A/ja active Pending
- 2020-01-15 MX MX2021008558A patent/MX2021008558A/es unknown
- 2020-01-15 CA CA3123052A patent/CA3123052A1/en active Pending
-
2021
- 2021-07-06 IL IL284637A patent/IL284637A/en unknown
-
2022
- 2022-09-23 US US17/951,614 patent/US12000811B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
BR112021011194A2 (pt) | 2021-08-31 |
KR20210116502A (ko) | 2021-09-27 |
US20200225199A1 (en) | 2020-07-16 |
US20230314388A1 (en) | 2023-10-05 |
US12000811B2 (en) | 2024-06-04 |
US11486864B2 (en) | 2022-11-01 |
CA3123052A1 (en) | 2020-07-23 |
EP3911947A1 (en) | 2021-11-24 |
CN113227785A (zh) | 2021-08-06 |
IL284637A (en) | 2021-08-31 |
AU2020208360A1 (en) | 2021-07-01 |
EA202191961A1 (ru) | 2021-12-13 |
JP2022517338A (ja) | 2022-03-08 |
SG11202106370WA (en) | 2021-07-29 |
WO2020150328A1 (en) | 2020-07-23 |
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