MX2022009929A - Microorganismo que comprende una variante lyse y metodo de produccion de un l-aminoacido usando la misma. - Google Patents

Microorganismo que comprende una variante lyse y metodo de produccion de un l-aminoacido usando la misma.

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Publication number
MX2022009929A
MX2022009929A MX2022009929A MX2022009929A MX2022009929A MX 2022009929 A MX2022009929 A MX 2022009929A MX 2022009929 A MX2022009929 A MX 2022009929A MX 2022009929 A MX2022009929 A MX 2022009929A MX 2022009929 A MX2022009929 A MX 2022009929A
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MX
Mexico
Prior art keywords
lyse
variant
amino acid
microorganism
same
Prior art date
Application number
MX2022009929A
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English (en)
Inventor
Sang Min Park
Boram Lim
Hyun Won Bae
Jaewon Jang
Yong Uk Shin
Hyo Jeong Byun
Moo Young Jung
Han Hyoung Lee
Yunjung Choi
Jihyun Shim
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of MX2022009929A publication Critical patent/MX2022009929A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporcionan un microorganismo que comprende una variante LysE y un método para producir un L-aminoácido usando la misma. La variante LysE puede mejorar la capacidad de excreción y/o producción de un L-aminoácido, en comparación con una silvestre.
MX2022009929A 2020-02-13 2021-02-10 Microorganismo que comprende una variante lyse y metodo de produccion de un l-aminoacido usando la misma. MX2022009929A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020200017559A KR102139806B1 (ko) 2020-02-13 2020-02-13 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법
PCT/KR2021/001793 WO2021162459A1 (ko) 2020-02-13 2021-02-10 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법

Publications (1)

Publication Number Publication Date
MX2022009929A true MX2022009929A (es) 2022-09-09

Family

ID=71839381

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022009929A MX2022009929A (es) 2020-02-13 2021-02-10 Microorganismo que comprende una variante lyse y metodo de produccion de un l-aminoacido usando la misma.

Country Status (11)

Country Link
US (1) US20230072150A1 (es)
EP (1) EP4105229A1 (es)
JP (1) JP7422237B2 (es)
KR (1) KR102139806B1 (es)
CN (1) CN113544141B (es)
AR (1) AR121142A1 (es)
AU (1) AU2021221374B2 (es)
BR (1) BR112022015945A2 (es)
MX (1) MX2022009929A (es)
WO (1) WO2021162459A1 (es)
ZA (1) ZA202207846B (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102139806B1 (ko) * 2020-02-13 2020-07-30 씨제이제일제당 (주) 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법
KR102703188B1 (ko) 2020-09-03 2024-09-06 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
JP2023540518A (ja) 2020-09-03 2023-09-25 デサン・コーポレイション Ｌ－リジン生産能が向上したコリネバクテリウムグルタミクム変異株およびこれを用いたｌ－リジンの生産方法
KR102277031B1 (ko) * 2021-01-29 2021-07-13 씨제이제일제당 (주) 신규한 타입 ii 시트레이트 신타아제 변이체 및 이를 이용한 l-라이신 생산 방법
JP2024509290A (ja) 2021-03-09 2024-02-29 デサン・コーポレイション Ｌ－リジン生産能が向上したコリネバクテリウム・グルタミカム変異株およびこれを用いたｌ－リジンの生産方法
CA3211636A1 (en) 2021-03-09 2022-09-15 Daesang Corporation Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same
KR20220126610A (ko) 2021-03-09 2022-09-16 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102685495B1 (ko) 2021-03-09 2024-07-16 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
CN113201555A (zh) * 2021-04-01 2021-08-03 云南师范大学 一种含有eGFP标记和潮霉素抗性双元载体的构建方法
MX2023012821A (es) 2021-04-29 2024-01-25 Cj Cheiljedang Corp Variante de corynebacterium glutamicum que tiene capacidad mejorada de producción de l-lisina, y procedimiento para producir l-lisina utilizando la misma.
KR20220148694A (ko) 2021-04-29 2022-11-07 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
AU2021443607A1 (en) 2021-04-30 2023-11-16 Cj Cheiljedang Corporation Corynebacterium glutamicum variant with improved l-lysine production ability, and method for producing l-lysine using same
KR20220149376A (ko) 2021-04-30 2022-11-08 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102682180B1 (ko) 2021-04-30 2024-07-05 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102668767B1 (ko) 2021-04-30 2024-05-24 씨제이제일제당 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
MX2023012825A (es) 2021-04-30 2024-01-25 Cj Cheiljedang Corp Variante de corynebacterium glutamicum con capacidad mejorada de producción de l-lisina y procedimiento de producción de l-lisina mediante el uso de la misma.
KR102703209B1 (ko) 2021-10-14 2024-09-05 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR20230059451A (ko) * 2021-10-26 2023-05-03 씨제이제일제당 (주) LysE 변이체 및 이를 이용한 L-아르기닌 생산방법
KR20230084993A (ko) 2021-12-06 2023-06-13 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR20230136447A (ko) * 2022-03-18 2023-09-26 씨제이제일제당 (주) 거짓쌀도둑거저리 유래 아스파테이트 1-디카복실레이스의 변이체 및 이를 포함하는 미생물
KR20230137154A (ko) 2022-03-21 2023-10-04 대상 주식회사 코리네박테리움 발현용 신규 프로모터 및 이의 용도
KR20240142277A (ko) 2023-03-20 2024-09-30 대상 주식회사 L-라이신 생산능이 향상된 변이 미생물 및 이를 이용한 l-라이신의 생산 방법

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Also Published As

Publication number Publication date
US20230072150A1 (en) 2023-03-09
WO2021162459A1 (ko) 2021-08-19
AU2021221374B2 (en) 2023-05-18
JP2023512499A (ja) 2023-03-27
CN113544141A (zh) 2021-10-22
JP7422237B2 (ja) 2024-01-25
CN113544141B (zh) 2023-10-31
ZA202207846B (en) 2023-12-20
AU2021221374A1 (en) 2022-07-14
EP4105229A1 (en) 2022-12-21
KR102139806B1 (ko) 2020-07-30
AR121142A1 (es) 2022-04-20
BR112022015945A2 (pt) 2022-10-04

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