MX2022009560A - Proceso de fabricacion de vectores virales adenoasociados. - Google Patents

Proceso de fabricacion de vectores virales adenoasociados.

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Publication number
MX2022009560A
MX2022009560A MX2022009560A MX2022009560A MX2022009560A MX 2022009560 A MX2022009560 A MX 2022009560A MX 2022009560 A MX2022009560 A MX 2022009560A MX 2022009560 A MX2022009560 A MX 2022009560A MX 2022009560 A MX2022009560 A MX 2022009560A
Authority
MX
Mexico
Prior art keywords
aav
nucleic acid
acid molecule
rep
making
Prior art date
Application number
MX2022009560A
Other languages
English (en)
Inventor
Ryan Cawood
Weiheng Su
Original Assignee
Oxford Genetics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2001486.6A external-priority patent/GB202001486D0/en
Priority claimed from GBGB2009241.7A external-priority patent/GB202009241D0/en
Priority claimed from GBGB2010835.3A external-priority patent/GB202010835D0/en
Priority claimed from GBGB2011437.7A external-priority patent/GB202011437D0/en
Application filed by Oxford Genetics Ltd filed Critical Oxford Genetics Ltd
Publication of MX2022009560A publication Critical patent/MX2022009560A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/861—Adenoviral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/864—Parvoviral vectors, e.g. parvovirus, densovirus
    • C12N15/8645—Adeno-associated virus
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
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    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
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    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Life Sciences & Earth Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La invención se refiere a una molécula de ácido nucleico que codifica al menos un polipéptido rep del AAV, en donde uno o más de los promotores p5, p19 y p40 de AAV se han modificado para reducir o eliminar la expresión de uno o más de los polipéptidos Rep, o la molécula de ácido nucleico no codifica polipéptidos Rep52 o Rep40 funcionales, o la molécula de ácido nucleico no codifica una secuencia inhibidora de adenovirus funcional. La invención también se refiere a un proceso para producir vectores AAV recombinantes mediante el uso de un sistema de 2 adenovirus, en donde todos los genes necesarios para la replicación y el empaquetamiento de AAV (es decir, una secuencia de rep del AAV de la invención, cap del AAV y el vector de transferencia de AAV que comprende un transgén) pueden estar codificados dentro de dos adenovirus.
MX2022009560A 2020-02-04 2021-02-03 Proceso de fabricacion de vectores virales adenoasociados. MX2022009560A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB2001486.6A GB202001486D0 (en) 2020-02-04 2020-02-04 Process for making adenoassociated viral vectors
GBGB2009241.7A GB202009241D0 (en) 2020-06-17 2020-06-17 Process for making adenoassociated virel vectors
GBGB2010835.3A GB202010835D0 (en) 2020-07-14 2020-07-14 Process for making adenoassociated viral vectors
GBGB2011437.7A GB202011437D0 (en) 2020-07-23 2020-07-23 Process for making adenoassociated viral vectors
PCT/GB2021/050235 WO2021156609A1 (en) 2020-02-04 2021-02-03 Process for making adenoassociated viral vectors

Publications (1)

Publication Number Publication Date
MX2022009560A true MX2022009560A (es) 2022-09-09

Family

ID=74592311

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022009560A MX2022009560A (es) 2020-02-04 2021-02-03 Proceso de fabricacion de vectores virales adenoasociados.

Country Status (11)

Country Link
US (1) US20230257770A1 (es)
EP (1) EP4100537A1 (es)
JP (1) JP2023513891A (es)
KR (1) KR20220137087A (es)
CN (1) CN115298316A (es)
AU (1) AU2021217799A1 (es)
BR (1) BR112022013581A2 (es)
CA (1) CA3162619A1 (es)
GB (1) GB2592751B (es)
MX (1) MX2022009560A (es)
WO (1) WO2021156609A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4112731A1 (en) * 2021-07-01 2023-01-04 Charité - Universitätsmedizin Berlin System for high-level raav production
CN114395574B (zh) * 2022-01-18 2023-08-11 长沙爱科博生物科技有限公司 一种猪流行性腹泻病毒融合蛋白及其编码基因与应用
WO2024046403A1 (zh) * 2022-08-31 2024-03-07 江苏金斯瑞蓬勃生物科技有限公司 能够提高腺相关病毒滴度的腺相关病毒结构质粒
WO2024121778A1 (en) * 2022-12-09 2024-06-13 Pfizer Inc. Improved rep genes for recombinant aav production
CN117247434B (zh) * 2023-11-10 2024-02-02 上海朗昇生物科技有限公司 衣壳修饰型病毒载体及其制备和用途
CN117778431A (zh) * 2023-12-04 2024-03-29 广州派真生物技术有限公司 一种用于重组腺相关病毒包装的质粒系统及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115391B1 (en) * 1999-10-01 2006-10-03 Genovo, Inc. Production of recombinant AAV using adenovirus comprising AAV rep/cap genes
IL260655B2 (en) * 2016-01-29 2023-03-01 Sirion Biotech Gmbh AAV-based conditional expression system
SG11201911572YA (en) 2017-07-25 2020-02-27 Oxford Genetics Ltd Adenoviral vectors
EP3456822A1 (en) * 2017-09-19 2019-03-20 CEVEC Pharmaceuticals GmbH Inducible aav rep genes
GB201800903D0 (en) * 2018-01-19 2018-03-07 Oxford Genetics Ltd Vectors

Also Published As

Publication number Publication date
CA3162619A1 (en) 2021-08-12
CN115298316A (zh) 2022-11-04
GB2592751A (en) 2021-09-08
US20230257770A1 (en) 2023-08-17
JP2023513891A (ja) 2023-04-04
WO2021156609A1 (en) 2021-08-12
BR112022013581A2 (pt) 2022-09-13
AU2021217799A1 (en) 2022-07-21
EP4100537A1 (en) 2022-12-14
GB202101508D0 (en) 2021-03-17
GB2592751B (en) 2024-03-27
KR20220137087A (ko) 2022-10-11

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