MX2022002710A - Cromatografia de cationes que usa la concentracion de sal prevista del amortiguador de elucion. - Google Patents

Cromatografia de cationes que usa la concentracion de sal prevista del amortiguador de elucion.

Info

Publication number
MX2022002710A
MX2022002710A MX2022002710A MX2022002710A MX2022002710A MX 2022002710 A MX2022002710 A MX 2022002710A MX 2022002710 A MX2022002710 A MX 2022002710A MX 2022002710 A MX2022002710 A MX 2022002710A MX 2022002710 A MX2022002710 A MX 2022002710A
Authority
MX
Mexico
Prior art keywords
salt concentration
target
elution buffer
elution
chromatography
Prior art date
Application number
MX2022002710A
Other languages
English (en)
Inventor
Andreas Schaubmar
Felix Wittkopp
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2022002710A publication Critical patent/MX2022002710A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/64—Electrical detectors
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/30—Prediction of properties of chemical compounds, compositions or mixtures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/04—Processes using organic exchangers
    • B01J39/07—Processes using organic exchangers in the weakly acidic form
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/02—Column or bed processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/067—Preparation by reaction, e.g. derivatising the sample
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/10—Analysis or design of chemical reactions, syntheses or processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Computing Systems (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

La presente invención se refiere a un método de cromatografía para producir un volumen de elución objetivo (238) que comprende una primera y una segunda proteína objetivo (P1, P2). El método comprende: proporcionar (102) una columna de cromatografía de intercambio catiónico (230); aplicar (104) una solución de proteína (202) sobre la columna, en donde la solución de proteína comprende la primera proteína objetivo (P1), una segunda proteína objetivo y, opcionalmente, una o más proteínas adicionales (P3, P4, P5, P6); ingresar (106) un criterio de optimización; calcular (108) simulaciones de cromatografía para calcular una concentración de sal del amortiguador de elución (222) adaptada para proporcionar un volumen de elución objetivo que mejor coincida con el criterio de optimización; calcular (110) los bordes de agrupamiento del volumen de elución objetivo como una función de al menos la concentración de sal calculada y el criterio de optimización de entrada; aplicar (112) un amortiguador de elución (226) que tiene la concentración de sal calculada en la columna de cromatografía; llevar a cabo (114) la elución; y recolectar (116) el volumen de elución objetivo calculado.
MX2022002710A 2019-09-25 2020-09-24 Cromatografia de cationes que usa la concentracion de sal prevista del amortiguador de elucion. MX2022002710A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19199438 2019-09-25
PCT/EP2020/025433 WO2021058132A1 (en) 2019-09-25 2020-09-24 Cation chromatography using predicted elution buffer salt concentration

Publications (1)

Publication Number Publication Date
MX2022002710A true MX2022002710A (es) 2022-03-22

Family

ID=68066721

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022002710A MX2022002710A (es) 2019-09-25 2020-09-24 Cromatografia de cationes que usa la concentracion de sal prevista del amortiguador de elucion.

Country Status (13)

Country Link
US (1) US20220187256A1 (es)
EP (1) EP4018453A1 (es)
JP (1) JP2022547203A (es)
KR (1) KR20220069942A (es)
CN (1) CN114341634B (es)
AR (1) AR120043A1 (es)
AU (1) AU2020356149A1 (es)
BR (1) BR112022003710A2 (es)
CA (1) CA3148467A1 (es)
IL (1) IL291123A (es)
MX (1) MX2022002710A (es)
TW (1) TW202128264A (es)
WO (1) WO2021058132A1 (es)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2145873C1 (ru) * 1993-09-21 2000-02-27 Хемосол Инк. Способ очистки гемоглобина в коммерческом масштабе
TWI320788B (en) * 2005-05-25 2010-02-21 Hoffmann La Roche Method for the purification of antibodies
CA2632519A1 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
WO2008028974A1 (en) * 2006-09-08 2008-03-13 Novo Nordisk A/S Methods of optimizing chromatographic separation of polypeptides
CA2711375C (en) * 2008-01-18 2019-04-16 F. Hoffmann-La Roche Ag Purification of not-glycosylated polypeptides
IN2012DN00338A (es) * 2009-08-07 2015-05-22 Millipore Corp
CA2890979A1 (en) * 2012-11-15 2014-05-22 Genentech, Inc. Ionic strength-mediated ph gradient ion exchange chromatography
US9821249B2 (en) * 2015-09-08 2017-11-21 Orochem Technologies, Inc. Continuous process for separation of proteins
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
US10294452B2 (en) * 2016-11-22 2019-05-21 Dao-Yao He Lysis, extraction and purification of adeno-associated virus and adenovirus from host cells

Also Published As

Publication number Publication date
CA3148467A1 (en) 2021-04-01
US20220187256A1 (en) 2022-06-16
TW202128264A (zh) 2021-08-01
IL291123A (en) 2022-05-01
AU2020356149A1 (en) 2022-03-10
CN114341634A (zh) 2022-04-12
BR112022003710A2 (pt) 2022-05-24
JP2022547203A (ja) 2022-11-10
KR20220069942A (ko) 2022-05-27
EP4018453A1 (en) 2022-06-29
CN114341634B (zh) 2024-01-23
AR120043A1 (es) 2022-01-26
WO2021058132A1 (en) 2021-04-01

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