MX2021015698A - Integración dirigida de configuraciones aleatorias de ácidos nucleicos. - Google Patents
Integración dirigida de configuraciones aleatorias de ácidos nucleicos.Info
- Publication number
- MX2021015698A MX2021015698A MX2021015698A MX2021015698A MX2021015698A MX 2021015698 A MX2021015698 A MX 2021015698A MX 2021015698 A MX2021015698 A MX 2021015698A MX 2021015698 A MX2021015698 A MX 2021015698A MX 2021015698 A MX2021015698 A MX 2021015698A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acids
- directed integration
- random configurations
- express
- proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962866893P | 2019-06-26 | 2019-06-26 | |
| US202062991708P | 2020-03-19 | 2020-03-19 | |
| PCT/US2020/039753 WO2020264253A1 (en) | 2019-06-26 | 2020-06-26 | Randomized configuration targeted integration of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2021015698A true MX2021015698A (es) | 2022-03-17 |
Family
ID=71728890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2021015698A MX2021015698A (es) | 2019-06-26 | 2020-06-26 | Integración dirigida de configuraciones aleatorias de ácidos nucleicos. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11634836B2 (https=) |
| EP (1) | EP3990649A1 (https=) |
| JP (2) | JP7720787B2 (https=) |
| KR (1) | KR20220025806A (https=) |
| CN (1) | CN114080454A (https=) |
| AU (1) | AU2020308002A1 (https=) |
| CA (1) | CA3141233A1 (https=) |
| IL (1) | IL289146A (https=) |
| MX (1) | MX2021015698A (https=) |
| TW (1) | TWI891643B (https=) |
| WO (1) | WO2020264253A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021262798A1 (en) * | 2020-06-24 | 2021-12-30 | Genentech, Inc. | Targeted integration of nucleic acids |
| CN117305334B (zh) * | 2022-06-21 | 2024-11-08 | 深圳太力生物技术有限责任公司 | 靶向整合细胞及其制备方法、生产目标基因表达产物的方法 |
| JP2026502534A (ja) * | 2023-01-12 | 2026-01-23 | ジェネンテック， インコーポレイテッド | 核酸の標的組込みのためのコンビナトリアルベクターのクローニングおよびトランスフェクション戦略 |
| CN116466074B (zh) * | 2023-03-20 | 2026-02-24 | 吉林大学 | 基于特异性抗原fhh的肝片吸虫胶体金检测方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2096222C (en) | 1990-11-13 | 1998-12-29 | Stephen D. Lupton | Bifunctional selectable fusion genes |
| WO1994028143A1 (en) | 1993-05-21 | 1994-12-08 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US7126041B1 (en) * | 1999-12-10 | 2006-10-24 | North Carolina State Unversity | High efficiency gene targeting in plants |
| WO2008106985A1 (en) * | 2007-03-07 | 2008-09-12 | Aarhus Universitet | Pig model |
| FR2927089B1 (fr) * | 2008-02-05 | 2011-03-25 | Inst Nat De La Rech Agronomique Inra | Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia |
| WO2014085711A1 (en) | 2012-11-30 | 2014-06-05 | Larix Biosciences Llc | A novel cell line screening method |
| BR112015031639A2 (pt) * | 2013-06-19 | 2019-09-03 | Sigma Aldrich Co Llc | integração alvo |
| US9932607B2 (en) * | 2013-11-15 | 2018-04-03 | The Board Of Trustees Of The Leland Stanford Junior University | Site-specific integration of transgenes into human cells |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| KR102243243B1 (ko) | 2014-10-23 | 2021-04-22 | 리제너론 파마슈티칼스 인코포레이티드 | 신규한 cho 통합 부위 및 이의 용도 |
| EA201892137A1 (ru) | 2016-04-20 | 2019-03-29 | Регенерон Фармасьютикалз, Инк. | Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию |
| US11512144B2 (en) | 2016-04-20 | 2022-11-29 | Regeneran Pharmaceuticals, Inc. | Compositions and methods for making antibodies based on use of an expression-enhancing loci |
| IL275462B2 (en) * | 2017-12-22 | 2026-01-01 | Genentech Inc | Targeted integration of nucleic acids |
| AU2019403279A1 (en) * | 2018-12-21 | 2021-07-01 | Genentech, Inc. | Targeted integration of nucleic acids |
-
2020
- 2020-06-26 WO PCT/US2020/039753 patent/WO2020264253A1/en not_active Ceased
- 2020-06-26 CN CN202080047399.2A patent/CN114080454A/zh active Pending
- 2020-06-26 MX MX2021015698A patent/MX2021015698A/es unknown
- 2020-06-26 AU AU2020308002A patent/AU2020308002A1/en not_active Abandoned
- 2020-06-26 EP EP20743418.4A patent/EP3990649A1/en active Pending
- 2020-06-26 KR KR1020227000914A patent/KR20220025806A/ko active Pending
- 2020-06-26 CA CA3141233A patent/CA3141233A1/en active Pending
- 2020-06-26 JP JP2021576250A patent/JP7720787B2/ja active Active
- 2020-06-29 TW TW109121966A patent/TWI891643B/zh active
-
2021
- 2021-12-19 IL IL289146A patent/IL289146A/en unknown
- 2021-12-27 US US17/562,950 patent/US11634836B2/en active Active
-
2023
- 2023-04-03 US US18/129,976 patent/US20230279583A1/en active Pending
-
2025
- 2025-04-25 JP JP2025073331A patent/JP2025121945A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020264253A1 (en) | 2020-12-30 |
| JP2025121945A (ja) | 2025-08-20 |
| US20230279583A1 (en) | 2023-09-07 |
| KR20220025806A (ko) | 2022-03-03 |
| JP2022538073A (ja) | 2022-08-31 |
| IL289146A (en) | 2022-02-01 |
| AU2020308002A1 (en) | 2022-01-20 |
| TW202115252A (zh) | 2021-04-16 |
| JP7720787B2 (ja) | 2025-08-08 |
| US11634836B2 (en) | 2023-04-25 |
| CN114080454A (zh) | 2022-02-22 |
| US20220119985A1 (en) | 2022-04-21 |
| TWI891643B (zh) | 2025-08-01 |
| CA3141233A1 (en) | 2020-12-30 |
| EP3990649A1 (en) | 2022-05-04 |
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