TWI891643B - 核酸之隨機化組態靶向整合 - Google Patents

核酸之隨機化組態靶向整合

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Publication number
TWI891643B
TWI891643B TW109121966A TW109121966A TWI891643B TW I891643 B TWI891643 B TW I891643B TW 109121966 A TW109121966 A TW 109121966A TW 109121966 A TW109121966 A TW 109121966A TW I891643 B TWI891643 B TW I891643B
Authority
TW
Taiwan
Prior art keywords
host cells
sois
certain embodiments
vector
sequence
Prior art date
Application number
TW109121966A
Other languages
English (en)
Chinese (zh)
Other versions
TW202115252A (zh
Inventor
夏藍 米沙海
布萊德利 Ｒ 斯內德克
Original Assignee
美商建南德克公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 美商建南德克公司 filed Critical 美商建南德克公司
Publication of TW202115252A publication Critical patent/TW202115252A/zh
Application granted granted Critical
Publication of TWI891643B publication Critical patent/TWI891643B/zh

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
TW109121966A 2019-06-26 2020-06-29 核酸之隨機化組態靶向整合 TWI891643B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962866893P 2019-06-26 2019-06-26
US62/866,893 2019-06-26
US202062991708P 2020-03-19 2020-03-19
US62/991,708 2020-03-19

Publications (2)

Publication Number Publication Date
TW202115252A TW202115252A (zh) 2021-04-16
TWI891643B true TWI891643B (zh) 2025-08-01

Family

ID=71728890

Family Applications (1)

Application Number Title Priority Date Filing Date
TW109121966A TWI891643B (zh) 2019-06-26 2020-06-29 核酸之隨機化組態靶向整合

Country Status (11)

Country Link
US (2) US11634836B2 (https=)
EP (1) EP3990649A1 (https=)
JP (2) JP7720787B2 (https=)
KR (1) KR20220025806A (https=)
CN (1) CN114080454A (https=)
AU (1) AU2020308002A1 (https=)
CA (1) CA3141233A1 (https=)
IL (1) IL289146A (https=)
MX (1) MX2021015698A (https=)
TW (1) TWI891643B (https=)
WO (1) WO2020264253A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021262798A1 (en) * 2020-06-24 2021-12-30 Genentech, Inc. Targeted integration of nucleic acids
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
JP2026502534A (ja) * 2023-01-12 2026-01-23 ジェネンテック， インコーポレイテッド 核酸の標的組込みのためのコンビナトリアルベクターのクローニングおよびトランスフェクション戦略
CN116466074B (zh) * 2023-03-20 2026-02-24 吉林大学 基于特异性抗原fhh的肝片吸虫胶体金检测方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201803986A (zh) * 2016-04-20 2018-02-01 再生元醫藥公司 基於使用增強表現基因座之製備抗體的組成物及方法
WO2019126634A2 (en) * 2017-12-22 2019-06-27 Genentech, Inc. Targeted integration of nucleic acids
WO2020132165A1 (en) * 2018-12-21 2020-06-25 Genentech, Inc. Targeted integration of nucleic acids

Family Cites Families (11)

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Publication number Priority date Publication date Assignee Title
CA2096222C (en) 1990-11-13 1998-12-29 Stephen D. Lupton Bifunctional selectable fusion genes
WO1994028143A1 (en) 1993-05-21 1994-12-08 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
US7126041B1 (en) * 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
WO2008106985A1 (en) * 2007-03-07 2008-09-12 Aarhus Universitet Pig model
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
WO2014085711A1 (en) 2012-11-30 2014-06-05 Larix Biosciences Llc A novel cell line screening method
BR112015031639A2 (pt) * 2013-06-19 2019-09-03 Sigma Aldrich Co Llc integração alvo
US9932607B2 (en) * 2013-11-15 2018-04-03 The Board Of Trustees Of The Leland Stanford Junior University Site-specific integration of transgenes into human cells
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
KR102243243B1 (ko) 2014-10-23 2021-04-22 리제너론 파마슈티칼스 인코포레이티드 신규한 cho 통합 부위 및 이의 용도
EA201892137A1 (ru) 2016-04-20 2019-03-29 Регенерон Фармасьютикалз, Инк. Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201803986A (zh) * 2016-04-20 2018-02-01 再生元醫藥公司 基於使用增強表現基因座之製備抗體的組成物及方法
WO2019126634A2 (en) * 2017-12-22 2019-06-27 Genentech, Inc. Targeted integration of nucleic acids
WO2020132165A1 (en) * 2018-12-21 2020-06-25 Genentech, Inc. Targeted integration of nucleic acids

Also Published As

Publication number Publication date
WO2020264253A1 (en) 2020-12-30
JP2025121945A (ja) 2025-08-20
US20230279583A1 (en) 2023-09-07
KR20220025806A (ko) 2022-03-03
JP2022538073A (ja) 2022-08-31
IL289146A (en) 2022-02-01
AU2020308002A1 (en) 2022-01-20
TW202115252A (zh) 2021-04-16
JP7720787B2 (ja) 2025-08-08
US11634836B2 (en) 2023-04-25
CN114080454A (zh) 2022-02-22
US20220119985A1 (en) 2022-04-21
MX2021015698A (es) 2022-03-17
CA3141233A1 (en) 2020-12-30
EP3990649A1 (en) 2022-05-04

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