MX2021014057A - Polipéptidos de sintasas de cannabinoides optimizados. - Google Patents

Polipéptidos de sintasas de cannabinoides optimizados.

Info

Publication number
MX2021014057A
MX2021014057A MX2021014057A MX2021014057A MX2021014057A MX 2021014057 A MX2021014057 A MX 2021014057A MX 2021014057 A MX2021014057 A MX 2021014057A MX 2021014057 A MX2021014057 A MX 2021014057A MX 2021014057 A MX2021014057 A MX 2021014057A
Authority
MX
Mexico
Prior art keywords
engineered variants
methods
optimized
cbdas
polypeptide
Prior art date
Application number
MX2021014057A
Other languages
English (en)
Inventor
Andrew Horwitz
Jeff Ubersax
Darren Platt
Jeff Wong
Original Assignee
Demetrix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Demetrix Inc filed Critical Demetrix Inc
Publication of MX2021014057A publication Critical patent/MX2021014057A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y121/00—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21)
    • C12Y121/03—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21) with oxygen as acceptor (1.21.3)
    • C12Y121/03008—Cannabidiolic acid synthase (1.21.3.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente descripción proporciona variantes manipuladas de un polipéptido de sintasa de ácido cannabidiólico (CBDAS) que comprende una secuencia de aminoácidos de SEC ID NO:3 con una o más sustituciones de aminoácidos, ácidos nucleicos que comprenden secuencias de nucleótidos que codifican las variantes manipuladas, métodos para producir células hospedadoras modificadas que comprenden los ácidos nucleicos, células hospedadoras modificadas que expresan las variantes manipuladas, métodos para producir cannabinoides o derivados de cannabinoides y métodos para tamizar variantes manipuladas del polipéptido de sintasa de ácido cannabidiólico (CBDAS).
MX2021014057A 2019-05-22 2020-05-19 Polipéptidos de sintasas de cannabinoides optimizados. MX2021014057A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962851560P 2019-05-22 2019-05-22
US201962906017P 2019-09-25 2019-09-25
US201962906551P 2019-09-26 2019-09-26
PCT/US2020/033555 WO2020236789A1 (en) 2019-05-22 2020-05-19 Optimized cannabinoid synthase polypeptides

Publications (1)

Publication Number Publication Date
MX2021014057A true MX2021014057A (es) 2022-02-21

Family

ID=70978679

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021014057A MX2021014057A (es) 2019-05-22 2020-05-19 Polipéptidos de sintasas de cannabinoides optimizados.

Country Status (11)

Country Link
US (1) US20220228130A1 (es)
EP (1) EP3972989A1 (es)
JP (1) JP2022534032A (es)
CN (1) CN114729337A (es)
AU (1) AU2020278665A1 (es)
BR (1) BR112021023218A2 (es)
CA (1) CA3140079A1 (es)
IL (1) IL288097A (es)
MX (1) MX2021014057A (es)
SG (1) SG11202111975UA (es)
WO (1) WO2020236789A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3931330A4 (en) 2019-02-25 2023-03-15 Ginkgo Bioworks, Inc. BIOSYNTHESIS OF CANNABINOIDS AND CANNABINOID PRECURSORS
CN110272900B (zh) * 2019-04-19 2024-03-26 中国人民解放军陆军军医大学 用于制备骨骼发育异常猪模型的sgRNA及其应用
WO2023288188A1 (en) * 2021-07-13 2023-01-19 Amyris, Inc. High efficency production of cannabigerolic acid and cannabidiolic acid
CN114657078B (zh) * 2022-01-27 2024-04-02 森瑞斯生物科技(深圳)有限公司 一种高产大麻二酚酸的酿酒酵母菌株构建方法和应用
CN114591923B (zh) * 2022-05-10 2022-08-30 森瑞斯生物科技(深圳)有限公司 大麻二酚酸合成酶突变体及其构建方法与应用
CN116622784B (zh) * 2023-02-14 2024-03-01 黑龙江八一农垦大学 一种大麻二酚酸合成酶的应用
CN116574700B (zh) * 2023-05-12 2023-11-14 黑龙江八一农垦大学 大麻二酚酸合成酶突变体及其应用
CN116904412B (zh) * 2023-07-25 2024-04-26 森瑞斯生物科技(深圳)有限公司 一种大麻二酚酸合成酶序列优化的酿酒酵母菌株构建方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7371558B2 (en) 2002-10-04 2008-05-13 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
AU2008334945C1 (en) 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
SG192545A1 (en) 2008-04-23 2013-08-30 Danisco Us Inc Isoprene synthase variants for improved microbial production of isoprene
ES2716231T3 (es) 2008-07-02 2019-06-11 Danisco Us Inc Procedimientos para producir isopreno
CA2902452C (en) * 2013-02-28 2018-09-04 Full Spectrum Laboratories Limited Chemical engineering processes and apparatus for the synthesis of compounds
KR101695848B1 (ko) * 2015-03-03 2017-01-13 한국과학기술원 진세노사이드 f2를 포함하는 비알코올성 간 질환 또는 인슐린 저항성의 예방 또는 치료용 조성물
AU2017250303B2 (en) * 2016-04-15 2019-09-26 Teewinot Technologies Limited Biosynthesis of cannabinoid prodrugs
EP3472142A1 (en) * 2016-06-16 2019-04-24 Teewinot Technologies Limited Methods for the manufacture of cannabinoid prodrugs, pharmaceutical formulations and their use
AU2018256863A1 (en) * 2017-04-27 2019-11-14 Regents Of The University Of California Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
AU2018301828A1 (en) * 2017-07-12 2020-01-30 Biomedican, Inc. Production of cannabinoids in yeast

Also Published As

Publication number Publication date
IL288097A (en) 2022-01-01
US20220228130A1 (en) 2022-07-21
AU2020278665A1 (en) 2021-12-02
EP3972989A1 (en) 2022-03-30
CA3140079A1 (en) 2020-11-26
CN114729337A (zh) 2022-07-08
WO2020236789A1 (en) 2020-11-26
JP2022534032A (ja) 2022-07-27
SG11202111975UA (en) 2021-11-29
BR112021023218A2 (pt) 2022-02-08

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